ABSTRACT
Data are given on the study of functional correlations of the pairs of organs (kidneys-liver and kidneys-lungs) that participate in provision of the functional system of secretion. The correlation of these pairs of organs contribution to the total secretion of metabolites as renal insufficiency grows is characterized by the S-shaped dependence, i.e. by a compensatory increase of the conjoined functions at the early stages and its depression at the graver stages of renal insufficiency. This approach makes it possible to determine the character, terms and significance of the participation of various organs in provision of metabolic homeostasis maintenance long before its failure and to reveal the main links in the pathogenesis of functional disorders of secretion.
Subject(s)
Tuberculosis, Renal/physiopathology , Chlormerodrin/metabolism , Homeostasis , Humans , Kidney/metabolism , Kidney Failure, Chronic/etiology , Liver/metabolism , Lung/metabolism , Mercury Radioisotopes , Tuberculosis, Renal/metabolismABSTRACT
Subcellular distribution of chlormerodrin-203Hg in the rat kidney has been studied. Two hours after i.v. injection of the radiopharmaceutical, kidney tissue homogenate was submitted to differential centrifugation to obtain cell organelles. Radioactivity distribution in relation to total radioactivity of kidney homogenate obtained in nine repeated experiments was as it follows (+/- SD): nuclei 3.5% (+/- 0.5); mitochondria 6.7% (+/- 0.6); microsomes 9.3% (+/- 0.8) and cytosol 38% (+/- 3.9). Specific activities expressed as counts/min/mg of protein were 915 (+/- 13%), 1232 (+/- 14%) and 1097 (+/- 14%) for mitochondria, microsomes and cytosol, respectively. Specific activity of the nuclei was 1445 (+/- 16%) counts/min/mg DNA. These results demonstrate that this imaging agent penetrates into the kidney cells where it remains mostly in the cytosol. A fraction of chlormerodrin-203Hg binds to microsomes, mitochondria and nuclei.
Subject(s)
Chlormerodrin/metabolism , Kidney/metabolism , Mercury Radioisotopes , Animals , Cell Nucleus/metabolism , Cytosol/metabolism , Microsomes/metabolism , Mitochondria/metabolism , Rats , Rats, Inbred StrainsSubject(s)
Chlormerodrin/metabolism , Hippurates/metabolism , Kidney/metabolism , Pentetic Acid/metabolism , Humans , KineticsABSTRACT
Serial macroautoradiograms were obtained to determine the distribution of 203Hg-chlormerodrin, 203Hg-nigrate and 99mTc-DMSA in kidney. In the study, normal rats were used and these three radiopharmaceuticals were injected intravenously. Initial images of 203Hg-chlormerodrin showed the accumulation in the outer cortex, but no significant radioactivity in the medullary. On the other hand, delayed images revealed radioactivity shifting in concentration from the outer cortex to the inner cortex. Distribution pattern of 203Hg-nitrate was similar to that of 203Hg-chlormerodrin. In contrast to 203Hg-chlormerodrin and 203Hg-nitrate, 99mTc-DMSA was retained in the outer cortex without temporal changes in the distribution.
