Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 1.127
Filter
1.
FASEB J ; 38(10): e23665, 2024 May 31.
Article in English | MEDLINE | ID: mdl-38780085

ABSTRACT

Chlorogenic acid improves diabetic symptoms, including inflammation, via the modulation of the gut microbiota. However, the mechanism by which the microbiota is regulated by chlorogenic acid remains unknown. In this study, we firstly explored the effects of chlorogenic acid on diabetic symptoms, colonic inflammation, microbiota composition, and microRNA (miRNA) expression in db/db mice. The results showed that chlorogenic acid decreased body weight, improved glucose tolerance and intestinal inflammation, altered gut microbiota composition, and upregulated the expression level of five miRNAs, including miRNA-668-3p, miRNA-467d-5p, miRNA-129-1-3p, miRNA-770-3p, and miRNA-666-5p in the colonic content. Interestingly, the levels of these five miRNAs were positively correlated with the abundance of Lactobacillus johnsonii. We then found that miRNA-129-1-3p and miRNA-666-5p promoted the growth of L. johnsonii. Importantly, miRNA-129-1-3p mimicked the effects of chlorogenic acid on diabetic symptoms and colonic inflammation in db/db mice. Furthermore, L. johnsonii exerted beneficial effects on db/db mice similar to those of chlorogenic acid. In conclusion, chlorogenic acid regulated the gut microbiota composition via affecting miRNA expression and ameliorated intestinal inflammation via the miRNA-microbe axis in db/db mice.


Subject(s)
Chlorogenic Acid , Gastrointestinal Microbiome , Inflammation , MicroRNAs , Animals , Chlorogenic Acid/pharmacology , MicroRNAs/genetics , MicroRNAs/metabolism , Mice , Gastrointestinal Microbiome/drug effects , Male , Inflammation/drug therapy , Inflammation/metabolism , Mice, Inbred C57BL , Diabetes Mellitus, Experimental/drug therapy , Diabetes Mellitus, Experimental/metabolism
2.
ACS Appl Mater Interfaces ; 16(22): 28209-28221, 2024 Jun 05.
Article in English | MEDLINE | ID: mdl-38778020

ABSTRACT

Diabetic chronic wounds are notoriously difficult to heal as a result of their susceptibility to infection. To address this issue, we constructed an innovated and adaptable solution in the form of injectable chitosan (CS) hydrogel, denoted as CCOD, with enhanced antibacterial and anti-inflammatory properties. This hydrogel is created through a Schiff base reaction that combines chitosan-grafted chlorogenic acid (CS-CGA) and oxidized hyaluronic acid (OHA) with deferoxamine (DFO) as a model drug. The combination of CS and CGA has demonstrated excellent antibacterial and anti-inflammatory properties, while grafting played a pivotal role in making these positive effects stable. These unique features make it possible to customize injectable hydrogel and fit any wound shape, allowing for more effective and personalized treatment of complex bacterial infections. Furthermore, the hydrogel system is not only effective against inflammation and bacterial infections but also possesses antioxidant and angiogenic abilities, making it an ideal solution for the repair of chronic wounds that have been previously thought of as unmanageable.


Subject(s)
Anti-Bacterial Agents , Anti-Inflammatory Agents , Chitosan , Chlorogenic Acid , Deferoxamine , Hyaluronic Acid , Hydrogels , Wound Healing , Chitosan/chemistry , Chitosan/pharmacology , Hyaluronic Acid/chemistry , Hyaluronic Acid/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Deferoxamine/chemistry , Deferoxamine/pharmacology , Wound Healing/drug effects , Hydrogels/chemistry , Hydrogels/pharmacology , Animals , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacology , Chlorogenic Acid/administration & dosage , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/administration & dosage , Mice , Humans , Oxidation-Reduction , Angiogenesis Inducing Agents/pharmacology , Angiogenesis Inducing Agents/chemistry , Angiogenesis Inducing Agents/administration & dosage , Angiogenesis Inducing Agents/therapeutic use , Neovascularization, Physiologic/drug effects , Staphylococcus aureus/drug effects , Angiogenesis
3.
Reprod Domest Anim ; 59(5): e14596, 2024 May.
Article in English | MEDLINE | ID: mdl-38757656

ABSTRACT

Chlorogenic acid (CGA) is an effective phenolic antioxidant that can scavenge hydroxyl radicals and superoxide anions. Herein, the protective effects and mechanisms leading to CGA-induced porcine parthenogenetic activation (PA) in early-stage embryos were investigated. Our results showed that 50 µM CGA treatment during the in vitro culture (IVC) period significantly increased the cleavage and blastocyst formation rates and improved the blastocyst quality of porcine early-stage embryos derived from PAs. Then, genes related to zygotic genome activation (ZGA) were identified and investigated, revealing that CGA can promote ZGA in porcine PA early-stage embryos. Further analysis revealed that CGA treatment during the IVC period decreased the abundance of reactive oxygen species (ROS), increased the abundance of glutathione and enhanced the activity of catalase and superoxide dismutase in porcine PA early-stage embryos. Mitochondrial function analysis revealed that CGA increased mitochondrial membrane potential and ATP levels and upregulated the mitochondrial homeostasis-related gene NRF-1 in porcine PA early-stage embryos. In summary, our results suggest that CGA treatment during the IVC period helps porcine PA early-stage embryos by regulating oxidative stress and improving mitochondrial function.


Subject(s)
Chlorogenic Acid , Embryo Culture Techniques , Embryonic Development , Mitochondria , Oxidative Stress , Parthenogenesis , Reactive Oxygen Species , Animals , Oxidative Stress/drug effects , Parthenogenesis/drug effects , Mitochondria/drug effects , Embryo Culture Techniques/veterinary , Chlorogenic Acid/pharmacology , Embryonic Development/drug effects , Reactive Oxygen Species/metabolism , Blastocyst/drug effects , Swine , Membrane Potential, Mitochondrial/drug effects , Antioxidants/pharmacology , Female , Glutathione/metabolism
4.
Int J Mol Sci ; 25(10)2024 May 10.
Article in English | MEDLINE | ID: mdl-38791228

ABSTRACT

Chemotherapeutic drugs are indispensable in cancer treatment, but their effectiveness is often lessened because of non-selective toxicity to healthy tissues, which triggers inflammatory pathways that are harmful to vital organs. In addition, tumors' resistance to drugs causes failures in treatment. Chlorogenic acid (5-caffeoylquinic acid, CGA), found in plants and vegetables, is promising in anticancer mechanisms. In vitro and animal studies have indicated that CGA can overcome resistance to conventional chemotherapeutics and alleviate chemotherapy-induced toxicity by scavenging free radicals effectively. This review is a summary of current information about CGA, including its natural sources, biosynthesis, metabolism, toxicology, role in combatting chemoresistance, and protective effects against chemotherapy-induced toxicity. It also emphasizes the potential of CGA as a pharmacological adjuvant in cancer treatment with drugs such as 5-fluorouracil, cisplatin, oxaliplatin, doxorubicin, regorafenib, and radiotherapy. By analyzing more than 140 papers from PubMed, Google Scholar, and SciFinder, we hope to find the therapeutic potential of CGA in improving cancer therapy.


Subject(s)
Chlorogenic Acid , Drug Resistance, Neoplasm , Neoplasms , Humans , Chlorogenic Acid/pharmacology , Chlorogenic Acid/therapeutic use , Neoplasms/drug therapy , Neoplasms/metabolism , Drug Resistance, Neoplasm/drug effects , Animals , Antineoplastic Agents/therapeutic use , Antineoplastic Agents/pharmacology
5.
J Agric Food Chem ; 72(21): 12270-12280, 2024 May 29.
Article in English | MEDLINE | ID: mdl-38743450

ABSTRACT

Allergenicity of soybean 7S protein (7S) troubles many people around the world. However, many processing methods for lowering allergenicity is invalid. Interaction of 7S with phenolic acids, such as chlorogenic acid (CHA), to structurally modify 7S may lower the allergenicity. Hence, the effects of covalent (C-I, periodate oxidation method) and noncovalent interactions (NC-I) of 7S with CHA in different concentrations (0.3, 0.5, and 1.0 mM) on lowering 7S allergenicity were investigated in this study. The results demonstrated that C-I led to higher binding efficiency (C-0.3:28.51 ± 2.13%) than NC-I (N-0.3:22.66 ± 1.75%). The C-I decreased the α-helix content (C-1:21.06%), while the NC-I increased the random coil content (N-1:24.39%). The covalent 7S-CHA complexes of different concentrations had lower IgE binding capacity (C-0.3:37.38 ± 0.61; C-0.5:34.89 ± 0.80; C-1:35.69 ± 0.61%) compared with that of natural 7S (100%), while the noncovalent 7S-CHA complexes showed concentration-dependent inhibition of IgE binding capacity (N-0.3:57.89 ± 1.23; N-0.5:46.91 ± 1.57; N-1:40.79 ± 0.22%). Both interactions produced binding to known linear epitopes. This study provides the theoretical basis for the CHA application in soybean products to lower soybean allergenicity.


Subject(s)
Antigens, Plant , Chlorogenic Acid , Glycine max , Immunoglobulin E , Soybean Proteins , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacology , Glycine max/chemistry , Glycine max/immunology , Immunoglobulin E/immunology , Soybean Proteins/chemistry , Soybean Proteins/immunology , Antigens, Plant/chemistry , Antigens, Plant/immunology , Humans , Food Hypersensitivity/immunology , Allergens/chemistry , Allergens/immunology , Protein Binding , Seed Storage Proteins/chemistry , Seed Storage Proteins/immunology
6.
Food Chem ; 452: 139551, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38723572

ABSTRACT

This study explored the mechanism of interaction between chlorogenic acid (CA) and protein fibrils (PF) as well as the effects of varying the CA/PF concentration ratio on antibacterial activity. Analysis of various parameters, such as ζ-potential, thioflavin T fluorescence intensity, surface hydrophobicity, and free sulfhydryl groups, revealed that the interaction between PF and CA altered the structure of PF. Fluorescence analysis revealed that hydrogen bonding and hydrophobic interactions were the primary interaction forces causing conformational rearrangement, resulting in a shorter, more flexible, and thicker fibril structure, as observed through transmission electron microscopy. Fourier-transform infrared spectroscopy, small-angle X-ray scattering, and X-ray diffraction analyses revealed that the characteristic fibril structure was destroyed when the CA/PF ratio exceeded 0.05. Notably, the CA-PF complexes inhibited the growth of Escherichia coli and Staphylococcus aureus and also exhibited antioxidant activity. Overall, this study expands the application prospects of CA and PF in the food industry.


Subject(s)
Anti-Bacterial Agents , Chlorogenic Acid , Escherichia coli , Soybean Proteins , Staphylococcus aureus , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacology , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & development , Escherichia coli/drug effects , Escherichia coli/growth & development , Soybean Proteins/chemistry , Soybean Proteins/pharmacology , Hydrophobic and Hydrophilic Interactions , Glycine max/chemistry , Glycine max/growth & development
7.
J Agric Food Chem ; 72(20): 11759-11772, 2024 May 22.
Article in English | MEDLINE | ID: mdl-38738668

ABSTRACT

This study aimed to investigate alterations in gut microbiota and metabolites mediated by wheat-resistant starch and its repair of gut barrier dysfunction induced by a high-fat diet (HFD). Structural data revealed that chlorogenic acid (CA)/linoleic acid (LA) functioned through noncovalent interactions to form a more ordered structure and fortify antidigestibility in wheat starch (WS)-CA/LA complexes; the resistant starch (RS) contents of WS-CA, WS-LA, and WS-CA-LA complexes were 23.40 ± 1.56%, 21.25 ± 1.87%, and 35.47 ± 2.16%, respectively. Dietary intervention with WS-CA/LA complexes effectively suppressed detrimental alterations in colon tissue morphology induced by HFD and repaired the gut barrier in ZO-1 and MUC-2 levels. WS-CA/LA complexes could augment gut barrier-promoting microbes including Parabacteroides, Bacteroides, and Muribaculum, accompanied by an increase in short-chain fatty acids (SCFAs) and elevated expression of SCFA receptors. Moreover, WS-CA/LA complexes modulated secondary bile acid metabolism by decreasing taurochenodeoxycholic, cholic, and deoxycholic acids, leading to the activation of bile acid receptors. Collectively, this study offered guiding significance in the manufacture of functional diets for a weak gut barrier.


Subject(s)
Chlorogenic Acid , Diet, High-Fat , Gastrointestinal Microbiome , Linoleic Acid , Mice, Inbred C57BL , Starch , Triticum , Chlorogenic Acid/metabolism , Chlorogenic Acid/pharmacology , Chlorogenic Acid/administration & dosage , Chlorogenic Acid/chemistry , Diet, High-Fat/adverse effects , Triticum/chemistry , Triticum/metabolism , Gastrointestinal Microbiome/drug effects , Animals , Male , Mice , Starch/metabolism , Starch/chemistry , Linoleic Acid/metabolism , Linoleic Acid/chemistry , Bacteria/classification , Bacteria/metabolism , Bacteria/genetics , Bacteria/drug effects , Bacteria/isolation & purification , Intestinal Mucosa/metabolism , Intestinal Mucosa/drug effects , Humans , Fatty Acids, Volatile/metabolism , Resistant Starch/metabolism
8.
Nutrients ; 16(7)2024 Mar 23.
Article in English | MEDLINE | ID: mdl-38612964

ABSTRACT

Chlorogenic acid (CGA) is a type of polyphenol compound found in rich concentrations in many plants such as green coffee beans. As an active natural substance, CGA exerts diverse therapeutic effects in response to a variety of pathological challenges, particularly conditions associated with chronic metabolic diseases and age-related disorders. It shows multidimensional functions, including neuroprotection for neurodegenerative disorders and diabetic peripheral neuropathy, anti-inflammation, anti-oxidation, anti-pathogens, mitigation of cardiovascular disorders, skin diseases, diabetes mellitus, liver and kidney injuries, and anti-tumor activities. Mechanistically, its integrative functions act through the modulation of anti-inflammation/oxidation and metabolic homeostasis. It can thwart inflammatory constituents at multiple levels such as curtailing NF-kB pathways to neutralize primitive inflammatory factors, hindering inflammatory propagation, and alleviating inflammation-related tissue injury. It concurrently raises pivotal antioxidants by activating the Nrf2 pathway, thus scavenging excessive cellular free radicals. It elevates AMPK pathways for the maintenance and restoration of metabolic homeostasis of glucose and lipids. Additionally, CGA shows functions of neuromodulation by targeting neuroreceptors and ion channels. In this review, we systematically recapitulate CGA's pharmacological activities, medicinal properties, and mechanistic actions as a potential therapeutic agent. Further studies for defining its specific targeting molecules, improving its bioavailability, and validating its clinical efficacy are required to corroborate the therapeutic effects of CGA.


Subject(s)
Chlorogenic Acid , Polyphenols , Chlorogenic Acid/pharmacology , Chlorogenic Acid/therapeutic use , Homeostasis , Antioxidants/pharmacology , Antioxidants/therapeutic use , Biological Availability
9.
J Mater Chem B ; 12(19): 4717-4723, 2024 May 15.
Article in English | MEDLINE | ID: mdl-38655651

ABSTRACT

Metal-organic frameworks (MOFs) possess a variety of interesting features related to their composition and structure that make them excellent candidates to be used in agriculture. However, few studies have reported their use as delivery agents of agrochemicals. In this work, the natural polyphenol chlorogenic acid (CGA) was entrapped via simple impregnation in the titanium aminoterephthalate MOF, MIL-125-NH2. A combination of experimental and computational techniques was used to understand and quantify the encapsulated CGA in MIL-125-NH2. Subsequently, CGA delivery studies were carried out in water at different pHs, showing a fast release of CGA during the first 2 h (17.3 ± 0.3% at pH = 6.5). In vivo studies were also performed against larvae of mealworm (Tenebrio molitor), evidencing the long-lasting insecticidal activity of CGA@MIL-125-NH2. This report demonstrates the potential of MOFs in the efficient release of agrochemicals, and paves the way to their study against in vivo models.


Subject(s)
Chlorogenic Acid , Insecticides , Metal-Organic Frameworks , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacology , Metal-Organic Frameworks/chemistry , Metal-Organic Frameworks/pharmacology , Insecticides/chemistry , Insecticides/pharmacology , Animals , Tenebrio/chemistry , Tenebrio/drug effects , Larva/drug effects
10.
Int J Mol Sci ; 25(8)2024 Apr 09.
Article in English | MEDLINE | ID: mdl-38673738

ABSTRACT

The high content of bioactive compounds in Aronia melanocarpa fruit offers health benefits. In this study, the anti-atherosclerotic effect of Aronia extracts was assessed. The impact on the level of adhesion molecules and the inflammatory response of human umbilical vein endothelial cells (HUVECs) was shown in relation to the chemical composition and the stage of ripening of the fruits. Samples were collected between May (green, unripe) and October (red, overripe) on two farms in Poland, which differed in climate. The content of chlorogenic acids, anthocyanins, and carbohydrates in the extracts was determined using HPLC-DAD/RI. The surface expression of ICAM-1 and VCAM-1 in HUVECs was determined by flow cytometry. The mRNA levels of VCAM-1, ICAM-1, IL-6, and MCP-1 were assessed using the quantitative real-time PCR method. The farms' geographical location was associated with the quantity of active compounds in berries and their anti-atherosclerotic properties. Confirmed activity for green fruits was linked to their high chlorogenic acid content.


Subject(s)
Atherosclerosis , Fruit , Human Umbilical Vein Endothelial Cells , Photinia , Plant Extracts , Photinia/chemistry , Humans , Plant Extracts/pharmacology , Plant Extracts/chemistry , Fruit/chemistry , Atherosclerosis/drug therapy , Vascular Cell Adhesion Molecule-1/genetics , Vascular Cell Adhesion Molecule-1/metabolism , Intercellular Adhesion Molecule-1/metabolism , Intercellular Adhesion Molecule-1/genetics , Anthocyanins/pharmacology , Anthocyanins/chemistry , Chemokine CCL2/genetics , Chemokine CCL2/metabolism , Chlorogenic Acid/pharmacology , Chlorogenic Acid/chemistry , Interleukin-6/metabolism , Interleukin-6/genetics
11.
J Control Release ; 369: 420-443, 2024 May.
Article in English | MEDLINE | ID: mdl-38575075

ABSTRACT

Wound healing involves distinct phases, including hemostasis, inflammation, proliferation, and remodeling, which is a complex and dynamic process. Conventional preparations often fail to meet multiple demands and provide prompt information about wound status. Here, a pH/ROS dual-responsive hydrogel (OHA-PP@Z-CA@EGF) was constructed based on oxidized hyaluronic acid (OHA), phenylboronic acid-grafted ε-polylysine (PP), chlorogenic acid (CA)-loaded ZIF-8 (Z-CA), and epidermal growth factor (EGF), which possesses intrinsic antibacterial, antioxidant, and angiogenic capacities. Due to the Schiff base and Phenylboronate ester bonds, the hydrogel exhibited excellent mechanical properties, strong adhesion, good biodegradability, high biocompatibility, stable rheological properties, and self-healing ability. Moreover, introducing Z-CA as an initiator and nanofiller led to the additional cross-linking of hydrogel through coordination bonds, which further improved the mechanical properties and antioxidant capabilities. Bleeding models of liver and tail amputations demonstrated rapid hemostatic properties of the hydrogel. Besides, the hydrogel regulated macrophage phenotypes via the NF-κB/JAK-STAT pathways, relieved oxidative stress, promoted cell migration and angiogenesis, and accelerated diabetic wound healing. The hydrogel also enabled real-time monitoring of the wound healing stages by colorimetric detection. This multifunctional hydrogel opens new avenues for the treatment and management of full-thickness diabetic wounds.


Subject(s)
Chlorogenic Acid , Hydrogels , Macrophages , Nanocomposites , Wound Healing , Wound Healing/drug effects , Animals , Chlorogenic Acid/administration & dosage , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacology , Hydrogels/chemistry , Nanocomposites/chemistry , Nanocomposites/administration & dosage , RAW 264.7 Cells , Mice , Macrophages/drug effects , Antioxidants/pharmacology , Antioxidants/chemistry , Antioxidants/administration & dosage , Male , Phenotype , Rats, Sprague-Dawley , Polylysine/chemistry , Hyaluronic Acid/chemistry
12.
Nano Lett ; 24(17): 5154-5164, 2024 May 01.
Article in English | MEDLINE | ID: mdl-38602357

ABSTRACT

Developing novel strategies for defeating osteoporosis has become a world-wide challenge with the aging of the population. In this work, novel supramolecular nanoagonists (NAs), constructed from alkaloids and phenolic acids, emerge as a carrier-free nanotherapy for efficacious osteoporosis treatment. These precision nanoagonists are formed through the self-assembly of berberine (BER) and chlorogenic acid (CGA), utilizing noncovalent electrostatic, π-π, and hydrophobic interactions. This assembly results in a 100% drug loading capacity and stable nanostructure. Furthermore, the resulting weights and proportions of CGA and BER within the NAs are meticulously controlled with strong consistency when the CGA/BER assembly feed ratio is altered from 1:1 to 1:4. As anticipated, our NAs themselves could passively target osteoporotic bone tissues following prolonged blood circulation, modulate Wnt signaling, regulate osteogenic differentiation, and ameliorate bone loss in ovariectomy-induced osteoporotic mice. We hope this work will open a new strategy to design efficient herbal-derived Wnt NAs for dealing with intractable osteoporosis.


Subject(s)
Berberine , Chlorogenic Acid , Osteoporosis , Osteoporosis/drug therapy , Animals , Mice , Berberine/pharmacology , Berberine/therapeutic use , Berberine/chemistry , Berberine/administration & dosage , Berberine/pharmacokinetics , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacology , Chlorogenic Acid/therapeutic use , Chlorogenic Acid/administration & dosage , Female , Humans , Osteogenesis/drug effects , Bone and Bones/drug effects , Bone and Bones/pathology , Nanostructures/chemistry , Nanostructures/therapeutic use
13.
J Food Sci ; 89(5): 3048-3063, 2024 May.
Article in English | MEDLINE | ID: mdl-38563092

ABSTRACT

Although the benefits of sugarcane polyphenol (SP) are well documented, its function in preventing photoaging has not yet been investigated. This study aimed to investigate the protective effects of SP in preventing ultraviolet (UV)-B-induced skin photoaging in Balb/c mice, as well as the underlying mechanism. Chlorogenic acid was determined to be the primary component of SP by using high-performance liquid chromatography-mass spectrometry. SP and chlorogenic acid were orally administrated to mice for 56 days, and UV-B radiation exposure was administered 14 days after SP and chlorogenic acid administration and lasted 42 days to cause photoaging. SP and chlorogenic acid administrations significantly alleviated the UV-B-induced mouse skin photoaging, as indicated by the decrease in epidermal thickness, increase in the collagen (COL) volume fraction, and elevation in type 1 and type 3 COL contents. Notably, both SP and chlorogenic acid effectively reversed the overexpression of matrix metalloproteinase induced by UV-B exposure in the mouse skin. Furthermore, SP and chlorogenic acid reduced the expression of receptor for advanced glycosylation end products in the mice; amplified the activities of antioxidant enzymes superoxide dismutase and catalase; reduced malondialdehyde levels; and decreased inflammatory cytokines interleukin 1ß, interleukin 6, and tumor necrosis factor α levels. SP could be a prospective dietary supplement for anti-photoaging applications due to its antioxidant, anti-inflammatory, and anti-glycosylation attributes, and chlorogenic acid might play a major role in these effects. PRACTICAL APPLICATION: This study can provide a scientific basis for the practical application of sugarcane polyphenols. We expect that sugarcane polyphenols can be used in food and beverage products to provide flavor while combating skin aging.


Subject(s)
Anti-Inflammatory Agents , Antioxidants , Chlorogenic Acid , Mice, Inbred BALB C , Polyphenols , Saccharum , Skin Aging , Skin , Ultraviolet Rays , Animals , Skin Aging/drug effects , Skin Aging/radiation effects , Polyphenols/pharmacology , Mice , Ultraviolet Rays/adverse effects , Antioxidants/pharmacology , Saccharum/chemistry , Skin/radiation effects , Skin/drug effects , Skin/metabolism , Chlorogenic Acid/pharmacology , Glycosylation/drug effects , Anti-Inflammatory Agents/pharmacology , Female , Plant Extracts/pharmacology , Malondialdehyde/metabolism , Superoxide Dismutase/metabolism
14.
Fitoterapia ; 175: 105956, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38604261

ABSTRACT

ATP citrate lyase (ACLY) is a key enzyme in glucolipid metabolism, and abnormally high expression of ACLY occurs in many diseases, including cancers, dyslipidemia and cardiovascular diseases. ACLY inhibitors are prospective treatments for these diseases. However, the scaffolds of ACLY inhibitors are insufficient with weak activity. The discovery of inhibitors with structural novelty and high activity continues to be a research hotpot. Acanthopanax senticosus (Rupr. & Maxim.) Harms is used for cardiovascular disease treatment, from which no ACLY inhibitors have ever been found. In this work, we discovered three novel ACLY inhibitors, and the most potent one was isochlorogenic acid C (ICC) with an IC50 value of 0.14 ± 0.04 µM. We found dicaffeoylquinic acids with ortho-dihydroxyphenyl groups were important features for inhibition by studying ten phenolic acids. We further investigated interactions between the highly active compound ICC and ACLY. Thermal shift assay revealed that ICC could directly bind to ACLY and improve its stability in the heating process. Enzymatic kinetic studies indicated ICC was a noncompetitive inhibitor of ACLY. Our work discovered novel ACLY inhibitors, provided valuable structure-activity patterns and deepened knowledge on the interactions between this targe tand its inhibitors.


Subject(s)
ATP Citrate (pro-S)-Lyase , Eleutherococcus , Eleutherococcus/chemistry , Molecular Structure , ATP Citrate (pro-S)-Lyase/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Enzyme Inhibitors/isolation & purification , Enzyme Inhibitors/chemistry , Chlorogenic Acid/pharmacology , Chlorogenic Acid/isolation & purification , Chlorogenic Acid/chemistry , Phytochemicals/pharmacology , Phytochemicals/isolation & purification , Phytochemicals/chemistry , Quinic Acid/analogs & derivatives , Quinic Acid/pharmacology , Quinic Acid/isolation & purification , Quinic Acid/chemistry , Hydroxybenzoates/pharmacology , Hydroxybenzoates/isolation & purification , Hydroxybenzoates/chemistry , Structure-Activity Relationship
15.
Fitoterapia ; 175: 105950, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38599338

ABSTRACT

The inhibitory effect against 5-α reductase of the ethyl acetate (EA) extract from Physalis angulata was evaluated in vitro using mouse prostate homogenates, and the suppression of benign prostatic hyperplasia (BPH) was assessed in a mouse model of testosterone-induced BPH. The EA extract exhibited a potentially inhibitory effect on 5-α reductase with an IC50 of 197 µg/ml. In BPH mice, the EA extract at a dose of 12 mg/kg was comparable to finasteride 5 mg/kg in suppressing BPH in terms of reducing absolute enlarged prostate weight (p < 0.05 vs. BPH group) and mitigating the hypertrophy of glandular elements and prostate connective tissue. Identification of chemical ingredients in the EA extract by UPLC-QTOF-MS revealed 37 substances belonging chiefly to flavonoids and physalins. Further quantification of the EA extract by HPLC-PDA methods revealed that chlorogenic acid, and rutin were the main components. Molecular docking studies of chlorogenic acid and rutin on 5-α reductase showed their high affinity to the enzyme with binding energies of -9.3 and - 9.2 kcal/mol, respectively compared with finasteride (- 10.3 kcal/mol). Additionally, chlorogenic acid inhibited 5-α reductase with an IC50 of 12.07 µM while rutin did not. The presence of chlorogenic acid in the EA extract may explain the inhibitory effects of the EA extract on 5-α reductase, and thus the suppression of BPH.


Subject(s)
5-alpha Reductase Inhibitors , Molecular Docking Simulation , Physalis , Plant Extracts , Prostatic Hyperplasia , Animals , Prostatic Hyperplasia/drug therapy , Male , Plant Extracts/pharmacology , Plant Extracts/chemistry , Mice , Physalis/chemistry , 5-alpha Reductase Inhibitors/pharmacology , 5-alpha Reductase Inhibitors/isolation & purification , Phytochemicals/pharmacology , Phytochemicals/isolation & purification , Molecular Structure , Chlorogenic Acid/pharmacology , Chlorogenic Acid/isolation & purification , Prostate/drug effects , Disease Models, Animal
16.
Food Funct ; 15(9): 4741-4762, 2024 May 07.
Article in English | MEDLINE | ID: mdl-38629635

ABSTRACT

In the contemporary era, heightened emphasis on health and safety has emerged as a paramount concern among individuals with food. The concepts of "natural" and "green" have progressively asserted dominance in the food consumption market. Consequently, through continuous exploration and development, an escalating array of natural bioactive ingredients is finding application in both nutrition delivery and the broader food industry. Chlorogenic acid (CGA), a polyphenolic compound widely distributed in various plants in nature, has garnered significant attention. Abundant research underscores CGA's robust biological activity, showcasing notable preventive and therapeutic efficacy across diverse diseases. This article commences with a comprehensive overview, summarizing the dietary sources and primary biological activities of CGA. These encompass antioxidant, anti-inflammatory, antibacterial, anti-cancer, and neuroprotective activities. Next, a comprehensive overview of the current research on nutrient delivery systems incorporating CGA is provided. This exploration encompasses nanoparticle, liposome, hydrogel, and emulsion delivery systems. Additionally, the article explores the latest applications of CGA in the food industry. Serving as a cutting-edge theoretical foundation, this paper contributes to the design and development of CGA in the realms of nutrition delivery and the food industry. Finally, the article presents informed speculations and considerations for the future development of CGA.


Subject(s)
Chlorogenic Acid , Food Industry , Chlorogenic Acid/chemistry , Chlorogenic Acid/pharmacology , Humans , Animals , Antioxidants/pharmacology , Antioxidants/chemistry , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/administration & dosage , Anti-Inflammatory Agents/chemistry , Drug Delivery Systems/methods
17.
Food Funct ; 15(10): 5224-5237, 2024 May 20.
Article in English | MEDLINE | ID: mdl-38623646

ABSTRACT

Dietary interventions are receiving increasing attention for maintaining host health and diminishing disease risk. This study endeavored to elucidate the intervention effect of chlorogenic acid coupled with extruded rice starch (CGA-ES) in mitigating lipid metabolism disorders induced by a high-fat diet (HFD) in rats. First, a significant increase in resistant starch (RS) and a decrease in the predicted glycemic index (pGI) were observed in CGA-ES owing to the formation of an ordered structure (Dm, single helix, and V-type crystalline structure) and partly released CGA. Compared to a physical mixture of starch and chlorogenic acid (CGA + S), CGA-ES showed a more potent effect in alleviating lipid metabolism disorders, manifesting as reduced levels of blood glucose, serum total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), alanine transaminase (ALT) and alkaline phosphatase (AKP), as well as body weight. It is correlated with an improvement in the gut microecology, featuring bacteria known for cholesterol reduction and butyrate production (Butyricicoccus, Bifidobacterium, Fusicatenibacter, Turicibacter, and Enterorhabdus), along with bile acid, butyrate and PG (PG (17:0/16:0) and PG (18:1/16:0)). The RS fraction of CGA-ES was found to be the main contributor. These findings would provide evidence for future studies to regulate lipid metabolism disorders, and even obesity using CGA-ES.


Subject(s)
Chlorogenic Acid , Gastrointestinal Microbiome , Lipid Metabolism , Obesity , Oryza , Rats, Sprague-Dawley , Starch , Animals , Oryza/chemistry , Rats , Male , Chlorogenic Acid/pharmacology , Lipid Metabolism/drug effects , Starch/metabolism , Starch/chemistry , Obesity/metabolism , Gastrointestinal Microbiome/drug effects , Diet, High-Fat , Blood Glucose/metabolism , Cholesterol/blood , Cholesterol/metabolism , Triglycerides/metabolism , Triglycerides/blood
18.
Cells ; 13(6)2024 Mar 19.
Article in English | MEDLINE | ID: mdl-38534386

ABSTRACT

Semen handling and cryopreservation induce oxidative stress that should be minimized. In this study, human semen was supplemented during cryopreservation with formulations of handmade liposomes and chlorogenic acid (CGA), an antioxidant compound. Zwitterionic (ZL), anionic (AL), and cationic (CL) liposomes were synthesized and characterized. Three aliquots of swim-up-selected sperm were incubated with ZL, AL, and CL (1:10,000), respectively. The percentages of sperm with progressive motility, high mitochondrial membrane potential (MMP; JC-1), double-stranded DNA (dsDNA acridine orange), and acrosome integrity (Pisum sativum agglutinin) were assessed. Then, human semen was frozen using both 1:10,000 ZL and CGA as follows: freezing medium/empty ZL (EL), freezing medium/empty ZL/CGA in the medium (CGA + EL), freezing medium/CGA loaded ZL (CGA), freezing medium (CTR). The same sperm endpoints were evaluated. ZL were the most tolerated and used for semen cryopreservation protocols. All the supplemented samples showed better endpoints versus CTR (p < 0.001). In particular, spermatozoa from the CGA and CGA + EL A samples showed increased motility, dsDNA, and acrosome integrity versus CTR and EL (p < 0.001; motility EL vs. CGA + EL p < 0.05). ZL and CGA can improve post-thaw sperm quality, acting on both cold shock effect management and oxidative stress. These findings open new perspectives on human and animal reproduction.


Subject(s)
Fertility Preservation , Semen Preservation , Animals , Humans , Male , Freezing , Chlorogenic Acid/pharmacology , Liposomes , Cryoprotective Agents/pharmacology , Semen Preservation/methods , Seeds , Spermatozoa , Cryopreservation/methods , Dietary Supplements
19.
Food Chem ; 446: 138866, 2024 Jul 15.
Article in English | MEDLINE | ID: mdl-38430769

ABSTRACT

Fresh-cut potatoes are prone to surface browning and physiological degradation. Chlorogenic acid (CGA), a natural phenolic antioxidant, has demonstrated preservative properties in various postharvest products. However, the underlying mechanisms of its application on maintaining quality remain unclear. Therefore, the effect of exogenous CGA treatment on quality deterioration of potato slices and the mechanisms involved were investigated. Results revealed CGA treatment retarded the browning coloration, suppressed microbial growth and inhibited the declines in starch, and ascorbic acid contents in potato slices. Meanwhile, the treatment activated the phenylpropanoid pathway but decreased the activities of phenolic decomposition-related enzymes such as polyphenol oxidase (PPO) and tyrosinase and downregulated StPPO expression. Moreover, the treated slices exhibited reduced accumulation of reactive oxygen species and increased activity of antioxidant enzymes. Additionally, they displayed enhanced 2,2-diphenyl-1-picrylhydrazyl radicals scavenging capacity and higher ATP levels. Therefore, these findings indicated that CGA treatment was effective for quality maintenance and antioxidant capacity enhancement in fresh-cut potatoes, thereby providing potential strategies for the preservation and processing of fresh-cut produce.


Subject(s)
Antioxidants , Solanum tuberosum , Antioxidants/metabolism , Chlorogenic Acid/pharmacology , Chlorogenic Acid/metabolism , Solanum tuberosum/metabolism , Phenols/metabolism , Ascorbic Acid/metabolism , Catechol Oxidase/metabolism
20.
Aging (Albany NY) ; 16(5): 4832-4840, 2024 Mar 09.
Article in English | MEDLINE | ID: mdl-38461437

ABSTRACT

Osteoporosis is a usual bone disease in aging populations, principally in postmenopausal women. Anti-resorptive and anabolic drugs have been applied to prevent and cure osteoporosis and are associated to a different of adverse effects. Du-Zhong is usually applied in Traditional Chinese Medicine to strengthen bone, regulate bone metabolism, and treat osteoporosis. Chlorogenic acid is a major polyphenol in Du-Zhong. In the current study, chlorogenic acid was found to enhance osteoblast proliferation and differentiation. Chlorogenic acid also inhibits the RANKL-induced osteoclastogenesis. Notably, ovariectomy significantly decreased bone volume and mechanical properties in the ovariectomized (OVX) rats. Administration of chlorogenic acid antagonized OVX-induced bone loss. Taken together, chlorogenic acid seems to be a hopeful molecule for the development of novel anti-osteoporosis treatment.


Subject(s)
Osteoclasts , Osteoporosis , Humans , Rats , Female , Animals , Chlorogenic Acid/pharmacology , Chlorogenic Acid/therapeutic use , Chlorogenic Acid/metabolism , Osteogenesis , Osteoporosis/metabolism , Osteoblasts/metabolism , Cell Differentiation
SELECTION OF CITATIONS
SEARCH DETAIL
...