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1.
Sci Rep ; 10(1): 6752, 2020 04 21.
Article in English | MEDLINE | ID: mdl-32317688

ABSTRACT

Few reports describe oxysterols in healthy children or in children with liver disease. We aimed to determine whether developmental changes in urinary and serum oxysterols occur during childhood, and to assess whether oxysterols might be biomarkers for pediatric liver disease. Healthy children enrolled as subjects (36 and 35 for urine and serum analysis, respectively) included neonates, infants, preschoolers, and school-age children, studied along with 14 healthy adults and 8 children with liver disease. We quantitated 7 oxysterols including 4ß-, 20(S)-, 22(S)-, 22(R)-, 24(S)-, 25-, and 27-hydroxycholesterol using liquid chromatography/electrospray ionization-tandem mass spectrometry. Urinary total oxysterols were significantly greater in neonates than in infants (P < 0.05), preschoolers (P < 0.001), school-age children (P < 0.001), or adults (P < 0.001), declining with age. Serum total oxysterols in neonates were significantly lower than in infants (P < 0.05), preschoolers (P < 0.001), school-age children (P < 0.05), or adults (P < 0.01). Compared with healthy children, total oxysterols and 24(S)-hydroxycholesterol in liver disease were significantly increased in both urine (P < 0.001 and P < 0.001, respectively) and serum (P < 0.001 and P < 0.05, respectively). Oxysterols in liver disease, particularly 24(S)-hydroxycholesterol, were greater in urine than serum. Oxysterols change developmentally and might serve as a biomarker for pediatric liver disease. To our knowledge, this is the first such report.


Subject(s)
Biliary Atresia/diagnosis , Choledochal Cyst/diagnosis , Cholestasis, Intrahepatic/diagnosis , Hepatitis, Autoimmune/diagnosis , Liver Failure, Acute/diagnosis , Oxysterols , Adolescent , Adult , Age Factors , Biliary Atresia/blood , Biliary Atresia/pathology , Biliary Atresia/urine , Biomarkers/blood , Biomarkers/urine , Case-Control Studies , Child , Child, Preschool , Choledochal Cyst/blood , Choledochal Cyst/pathology , Choledochal Cyst/urine , Cholestasis, Intrahepatic/blood , Cholestasis, Intrahepatic/pathology , Cholestasis, Intrahepatic/urine , Female , Hepatitis, Autoimmune/blood , Hepatitis, Autoimmune/pathology , Hepatitis, Autoimmune/urine , Humans , Infant , Infant, Newborn , Liver/metabolism , Liver/pathology , Liver Failure, Acute/blood , Liver Failure, Acute/pathology , Liver Failure, Acute/urine , Male , Middle Aged , Oxysterols/blood , Oxysterols/urine , Spectrometry, Mass, Electrospray Ionization
2.
Clin Chim Acta ; 497: 67-75, 2019 Oct.
Article in English | MEDLINE | ID: mdl-31276634

ABSTRACT

BACKGROUND: Asymptomatic hypercholanemia of pregnancy (AHP) is a controversial hypercholanemia, which is difficult to distinguish from intrahepatic cholestasis of pregnancy (ICP). Our aim is to elucidate the characteristics of urinary bile acid (BA) profiling of women with AHP and to find potential biomarkers for the diagnosis and differential diagnosis of AHP. METHODS: We developed a pseudo-targeted approach to perform metabolomics analysis of bile acids (BAs) using ultra-high performance liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Urinary BAs profiles were compared among AHP women (n = 20), ICP patients (n = 33) and normal controls (n = 35). RESULTS: The profiling of urinary BAs was significantly different among the AHP, ICP and control groups. Compared to the control group, the AHP group had higher levels of four possible sulfated BAs and trihydroxy BAs, including the species of muricholic acid (MCA), cholic acid (CA) and six possible BAs, whereas, 20 possible sulfated BAs, taurochenodeoxycholic acid (TCDCA), tetrahydrocannabinolic acid (THCA), and seven possible BAs were significantly lower in the AHP group than those in the ICP group. Based on the receiver operating characteristic (ROC) analysis, glycocholic acid (GCA) combined with T-ω-MCA were found to be the potential combination biomarker for the diagnosis (area under the curve was 0.960) of AHP, and mono-S, Gtri-S-2 combined with TLCA-S were found to be the potential combination biomarker for the differential diagnosis (area under the curve was 0.990) of AHP and ICP. CONCLUSIONS: The metabolisms of urinary Bas were altered in the AHP group compared with the ICP group and the control group. Urinary BA profiling analysis can serve as an effective tool for the diagnosis of AHP and the differential diagnosis of AHP and ICP.


Subject(s)
Bile Acids and Salts/metabolism , Bile Acids and Salts/urine , Cholestasis, Intrahepatic/metabolism , Cholestasis, Intrahepatic/urine , Metabolomics , Pregnancy Complications/metabolism , Pregnancy Complications/urine , Case-Control Studies , Cholestasis, Intrahepatic/diagnosis , Chromatography, High Pressure Liquid , Female , Humans , Mass Spectrometry , Multivariate Analysis , Pregnancy , Pregnancy Complications/diagnosis , Time Factors
3.
Clin Chim Acta ; 471: 292-297, 2017 Aug.
Article in English | MEDLINE | ID: mdl-28669684

ABSTRACT

BACKGROUND: Intrahepatic cholestasis of pregnancy (ICP), a pregnancy-related liver disease, leads to complications for both mothers and fetuses. Metabolomic approach has been applied to maternal-fetal medicine. The global metabolomic alterations that are specific in ICP as yet have not been investigated. METHODS: Based on high performance liquid chromatography/hybrid quadrupole time-of-flight (HPLC/Q-TOF) mass spectrometry, the untargeted metabolomics was used to analyze the changes of urinary metabolites between ICP group and the control group. RESULTS: One hundred nine variables in positive model and 119 variables in negative model were significantly different (p<0.05) between the ICP group and the control group, with the VIP (variable importance in the project) score>1 by the orthogonal partial least squares discriminant analysis (OPLS-DA). 14 metabolites in positive model and 18 metabolites in negative model were selected and identified based on HMDB (human metabolome database). Most of these metabolites were involved in bile acids biosynthesis and metabolism, hormone metabolism and lipid metabolism. A metabolite panel (MG (22:5), LysoPE (22:5), l-homocysteine sulfonic acid, glycocholic acid and chenodeoxycholic acid 3-sulfate) was contrusted by the binary logistic regression analysis with high diagnostic accuracy for ICP. The area under the receiver operating characteristic curve was 0.988 with the sensitivity of 90.0% and specificity of 93.3%. CONCLUSIONS: Urinary metabolites allow for the discrimination of ICP from the controls by orthogonal partial least squares discriminant analysis. Therefore, these findings may provide deep insights for the etiopathogenesis of ICP. Moreover, the maternal urinary metabolite panel has the potential to be used as non-invasive biomarkers for the diagnosis of ICP.


Subject(s)
Cholestasis, Intrahepatic/urine , Pregnancy Complications/urine , Adult , Case-Control Studies , Cholestasis, Intrahepatic/diagnosis , Cholestasis, Intrahepatic/metabolism , Chromatography, High Pressure Liquid , Female , Humans , Mass Spectrometry , Metabolome , Multivariate Analysis , Pregnancy , Pregnancy Complications/diagnosis , Pregnancy Complications/metabolism
4.
J Lipid Res ; 58(3): 607-614, 2017 03.
Article in English | MEDLINE | ID: mdl-28073941

ABSTRACT

Tetrahydroxy bile acids (THBAs) are hydrophilic and are present at minimal or undetectable levels in healthy human adults, but are present at high levels in bile salt export pump (abcb11)-knockout mice. The roles of THBAs in human cholestatic diseases are unclear. We aimed to investigate the presence of THBAs in patients with infantile intrahepatic cholestasis and its correlation with outcome. Urinary bile acids (BAs) were analyzed by GC-MS. Data were compared between good (n = 21) (disease-free before 1 year old) and poor prognosis groups (n = 19). Good prognosis patients had a higher urinary THBA proportion than poor prognosis patients [25.89% (3.45-76.73%) vs. 1.93% (0.05-48.90%)]. A urinary THBA proportion >7.23% predicted good prognosis with high sensitivity (95.24%), specificity (84.21%), and area under the curve (0.91) (P < 0.0001). A THBA proportion 7.23% was an independent factor for decreased transplant-free survival (hazard ratio = 7.16, confidence interval: 1.24-41.31, P = 0.028). Patients with a confirmed ABCB11 or tight junction protein 2 gene mutation (n = 7) had a minimally detectable THBA proportion (0.23-2.99% of total BAs). Three patients with an ATP8B1 mutation had an elevated THBA proportion (7.51-37.26%). In conclusion, in addition to disease entity as a major determinant of outcome, a high THBA level was associated with good outcome in the infantile intrahepatic cholestasis patients.


Subject(s)
ATP-Binding Cassette Transporters/genetics , Adenosine Triphosphatases/genetics , Bile Acids and Salts/urine , Cholestasis, Intrahepatic/urine , Zonula Occludens-2 Protein/genetics , ATP Binding Cassette Transporter, Subfamily B, Member 11 , Bile Acids and Salts/genetics , Cholestasis, Intrahepatic/genetics , Cholestasis, Intrahepatic/pathology , Female , Humans , Infant , Male , Mutation , Prognosis
5.
Sci Rep ; 6: 31535, 2016 08 18.
Article in English | MEDLINE | ID: mdl-27534581

ABSTRACT

Intrahepatic cholestasis of pregnancy (ICP), a pregnancy-related liver disease, leads to complications for both mother and fetus. Circulating microRNAs (miRNAs) have emerged as candidate biomarkers for many diseases. So far, the circulating miRNAs profiling of ICP has not been investigated. To assess the urinary miRNAs as non-invasive biomarkers for ICP, a differential miRNA profiling was initially analyzed by individual quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assay in urinary samples from a screening set including 10 ICP and 10 healthy pregnancies. The selected candidate miRNAs were then validated by a validation set with 40 ICP and 50 healthy pregnancies using individual qRT-PCR assay. Compared with the expression in urine of healthy pregnant women, the expression levels of hsa-miR-151-3p and hsa-miR-300 were significantly down-regulated, whereas hsa-miR-671-3p and hsa-miR-369-5p were significantly up-regulated in urine from ICP patients (p < 0.05 and false discovery rate < 0.05). A binary logistic regression model was constructed using the four miRNAs. The area under the receiver operating characteristic curve was 0.913 (95% confidence interval = 0.847 to 0.980; sensitivity = 82.9%, specificity = 87.0%). Therefore, urinary microRNA profiling detection in ICP is feasible and maternal urinary miRNAs have the potential to be non-invasive biomarkers for the diagnosis of ICP.


Subject(s)
Cholestasis, Intrahepatic/diagnosis , Cholestasis, Intrahepatic/urine , MicroRNAs/urine , Pregnancy Complications/diagnosis , Pregnancy Complications/urine , Real-Time Polymerase Chain Reaction/methods , Reverse Transcriptase Polymerase Chain Reaction/methods , Adult , Biomarkers/urine , Female , Humans , Pregnancy
6.
Clin Chim Acta ; 413(15-16): 1301-4, 2012 Aug 16.
Article in English | MEDLINE | ID: mdl-22525741

ABSTRACT

BACKGROUND: Benign recurrent intrahepatic cholestasis type 1 (BRIC-1), a rare autosomal recessive disorder characterized by recurrent episodes of jaundice and pruritus, is caused by mutations in the ATP8B1 gene. Rifampicin has been reported to be an effective treatment of jaundice and pruritus in patients with BRIC. Proposed mechanisms of effect for rifampicin include enhancement of multidrug-resistance protein 2 expression, activation of the enzymes of uridine diphosphate glucuronosyltransferase 1A1 and cytochrome P450 3A4, and stimulation of 6α-hydroxylation of bile acids. METHODS: To confirm the diagnosis of BRIC-1 and demonstrate the effect of rifampicin treatment on bile acid metabolism, we analyzed the patient's ATP8B1 gene and bile acids in urine. RESULTS: We detected 2 heterozygous mutations in the ATP8B1 gene, and increasing amounts of unusual bile acids such as 1ß-hydroxylated cholic acid, 2ß-hydroxylated cholic acid, 4ß-hydroxylated cholic acid, 6α-hydroxylated cholic acid, and hyocholic acid in urine during rifampicin treatment. CONCLUSIONS: We diagnosed a jaundiced pediatric patient with BRIC-1 caused by 2 novel mutations (1226delA/2210delA) in the ATP8B1 gene. Rifampicin was effective in treating cholestasis. Results of urinary bile acid analyses during rifampicin treatment in this patient, suggested that rifampicin might stimulate 1ß-, 2ß-, and 4ß-hydroxylation of bile acids in addition to 6α-hydroxylation.


Subject(s)
Adenosine Triphosphatases/genetics , Cholestasis, Intrahepatic/drug therapy , Cholestasis, Intrahepatic/urine , Rifampin/therapeutic use , Bile Acids and Salts , Child , Cholestasis/drug therapy , Cholestasis/genetics , Cholestasis, Intrahepatic/genetics , Cholic Acid/metabolism , Cholic Acid/urine , Cholic Acids/metabolism , Cholic Acids/urine , Female , Humans , Hydroxylation , Mutation , Rifampin/pharmacology
8.
Dig Liver Dis ; 41(9): 683-9, 2009 Sep.
Article in English | MEDLINE | ID: mdl-19185551

ABSTRACT

BACKGROUND: Neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) is a novel inborn error of metabolism due to dysfunction of citrin protein, and much more information about this new disease is still needed for its clinical management. AIMS: To investigate in detail the clinical and laboratory features of NICCD. PATIENTS: 13 NICCD subjects in mainland of China diagnosed in our department since 2006. METHODS: The anthropometric parameters of the patients at birth were compared with controls, representative biochemical changes and metabolome findings were investigated cross-sectionally, and mutations in the causative gene SLC25A13 were analyzed by protocols established previously. RESULTS: The patients showed reduced birth weight, length and ponderal index. Main clinical manifestations consisted of jaundice, hepato/hepatosplenomegaly and steatohepatosis on ultrasonography. Biochemical analysis revealed intrahepatic cholestasis, delayed switch of AFP to albumin, and elevated triglyceride, total cholesterol and LDL-cholesterol together with reduced HDL-cholesterol. Metabolome findings included co-existence of markers for galactosemia and tyrosinemia in urine, and elevated Cit, Met, Thr, Tyr, Lys, Arg and Orn in blood. Mutations of 851-854del, IVS6+5G>A, 1638-1660dup, A541D, IVS16ins3kb, R319X and G333D were detected in the gene SLC25A13. CONCLUSIONS: The diagnosis of NICCD cannot be established based just on the numerous but non-specific clinical manifestations and biochemical changes. The relatively specific metabolome features provide valuable tools for its screening and diagnosis, while SLC25A13 mutation analysis should be taken as one of the reliable tools for the definitive diagnosis. The body proportionality at birth, steatohepatosis on ultrasonography, delayed switch of AFP to albumin, dyslipidemia pattern, urinary metabolome features and the novel mutation G333D expanded the clinical spectrum of NICCD.


Subject(s)
Calcium-Binding Proteins/deficiency , Cholestasis, Intrahepatic/etiology , Metabolism, Inborn Errors/complications , Mitochondrial Membrane Transport Proteins/genetics , Organic Anion Transporters/deficiency , Case-Control Studies , China , Cholestasis, Intrahepatic/epidemiology , Cholestasis, Intrahepatic/genetics , Cholestasis, Intrahepatic/urine , Cross-Sectional Studies , Female , Fetal Growth Retardation/epidemiology , Humans , Infant , Infant, Newborn , Male , Metabolism, Inborn Errors/urine , Mutation
9.
Toxicol Pathol ; 36(6): 818-26, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18776161

ABSTRACT

Urinary metabolic fingerprinting with Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) was performed to monitor metabolic changes in an alpha-naphthylisothiocyanate (ANIT)-induced rat model of intrahepatic cholestasis and to investigate the relationships among metabolic changes, histopathology, and blood chemistry. ANIT was administered orally as a single dose of 100 mg/kg. Urine samples were collected predose (-31 to -24 hours) and postdose at 0-7, 7-24, 24-31, 31-48, 48-55, 55-72, and 72-96 hours, and serum samples were collected on days 1, 2, and 4 postdose. Increased levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and total bilirubin were seen on day 2. The negative ion profiles for urine samples collected after 7-24, 24-31, 31-48, and 48-55 hours differed from the predose profile based on principal component analysis. Onset of recovery was observed after 24-31 hours, when the urinary composition reverted toward the predose position. In conclusion, it is possible to monitor the progression of and recovery from drug-induced hepatotoxicity by urinary metabolic fingerprinting with FT-ICR MS and to search for potential biomarkers involved in intrahepatic cholestasis.


Subject(s)
1-Naphthylisothiocyanate/toxicity , Biomarkers/urine , Cholestasis, Intrahepatic/chemically induced , 1-Naphthylisothiocyanate/administration & dosage , Alanine Transaminase/blood , Alkaline Phosphatase/blood , Animals , Aspartate Aminotransferases/blood , Bilirubin/blood , Biomarkers/chemistry , Cholestasis, Intrahepatic/blood , Cholestasis, Intrahepatic/pathology , Cholestasis, Intrahepatic/urine , Cyclotrons , Fourier Analysis , Liver/pathology , Male , Mass Spectrometry , Noxae/administration & dosage , Noxae/toxicity , Principal Component Analysis , Rats
10.
Hepatology ; 47(2): 544-51, 2008 Feb.
Article in English | MEDLINE | ID: mdl-17968976

ABSTRACT

UNLABELLED: Intrahepatic cholestasis of pregnancy (ICP) is characterized by pruritus, elevated bile acids, and, specifically, elevated disulphated progesterone metabolites. We aimed to study changes in these parameters during treatment with dexamethasone or ursodeoxycholic acid (UDCA) in 40 out of 130 women included in the Swedish ICP intervention trial (26 randomized to placebo or UDCA, 14 randomized to dexamethasone). Serum bile acid profiles and urinary steroid hormone metabolites were analyzed using isotope-dilution gas chromatography-mass spectrometry and electrospray-mass spectrometry. We found that all patients displayed ICP-typical serum bile acid profiles with >50% cholic acid at baseline but almost 80% UDCA upon treatment with this bile acid. In UDCA-treated patients, relative amounts of disulphated progesterone metabolites in urine decreased by 34%, 48% (P < 0.05), and 55% (P < 0.05) after 1, 2, and 3 weeks of treatment, respectively, which was significantly correlated to improvements of pruritus scores but not to serum bile acid levels. In contrast, in patients randomized to dexamethasone or placebo, no changes in steroid metabolites or pruritus scores were observed. CONCLUSION: UDCA treatment in ICP decreased urinary excretion of disulphated progesterone metabolites, suggesting that amelioration of pruritus is connected to stimulation of hepatobiliary excretion of progesterone disulphates.


Subject(s)
Bile Acids and Salts/blood , Cholestasis, Intrahepatic/drug therapy , Dexamethasone/therapeutic use , Pregnancy Complications/drug therapy , Progesterone/urine , Ursodeoxycholic Acid/therapeutic use , Adult , Cholestasis, Intrahepatic/blood , Cholestasis, Intrahepatic/urine , Female , Gestational Age , Humans , Placebos , Pregnancy , Pregnancy Complications/blood , Pregnancy Complications/urine , Pruritus/etiology , Pruritus/pathology , Spectrometry, Mass, Electrospray Ionization , Steroids/blood , Steroids/urine
11.
J Perinat Med ; 35(6): 486-91, 2007.
Article in English | MEDLINE | ID: mdl-18052835

ABSTRACT

AIMS: To determine sensitivity and specificity of urine bile acid sulfate (UBAS) and non-sulfated urine bile acid (UBA) assays for detection of intrahepatic cholestasis of pregnancy (ICP). METHODS: Urine was collected prospectively from healthy and pruritic patients evaluated for ICP. Results were expressed as a ratio to urinary creatinine. RESULTS: Of 20 patients evaluated for ICP, 14 had confirmed ICP by serum testing and six had pruritus only. UBAS results were higher with ICP (P<0.001) and increased with gestational age in healthy controls. Adjusted for gestational age, the multiples of the median (MoM) were still higher (2.64+/-1.11, 1.05+/-0.54, P<0.001). The sensitivity was 100% and specificity 83% at 1.5 MoM in pruritic patients. UBA results were higher with ICP (23.0+/-9.8, 12.8+/-7.4, P<0.001), with sensitivity of 100% and specificity of 50% at 10.2 micromol/g cretinine. CONCLUSION: Urinary bile acids can detect or exclude ICP with serum abnormalities. Urine bile acid sulfates have higher specificity than non-sulfated urine bile acids at equivalent sensitivities, but co-detection of progestin sulfates is suspected.


Subject(s)
Bile Acids and Salts/urine , Cholestasis, Intrahepatic/diagnosis , Pregnancy Complications/diagnosis , Adult , Case-Control Studies , Cholestasis, Intrahepatic/urine , Female , Humans , Pregnancy , Pregnancy Complications/urine , Sensitivity and Specificity
12.
Clin Endocrinol (Oxf) ; 66(4): 518-23, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17371469

ABSTRACT

OBJECTIVE: To analyse aspects of mineral metabolism, bone mineral density (BMD), bone remodelling activity and serum IGF-1 levels in children with chronic cholestatic disease (CCLD). PATIENTS AND MEASUREMENTS: A total of 13 children with chronic cholestatic liver disease (CCLD; mean age 7.2 +/- 4.8 years) and 22 control subjects (mean age 7.6 +/- 4.5 years) were studied. Serum osteocalcin, bone alkaline phosphatase (BAP), 25-hydroxyvitamin D, PTH and IGF-1 levels and urinary deoxypyridinoline were determined. BMD was measured by dual-energy X-ray absorptiometry in the lumbar spine, total hip and whole body. Lumbar spine areal BMD was converted mathematically to apparent volumetric BMD (aBMD) and corrected for the bone age of the patient. RESULTS: Z-score of lumbar spine BMD was lower in CCLD patients than in controls and the difference was maintained when BMD was expressed as aBMD (control = 0.107 +/- 0.02 vs. CCLD = 0.092 +/- 0.02 g/cm(3), P < 0.05) and after conversion for bone age. All participants showed normal 25-hydroxyvitamin D levels, with no significant differences in serum levels of 25-hydroxyvitamin D and PTH between groups. IGF-1 levels were significantly lower in the CCLD group (control = 19.6 +/- 16.8 vs. CCLD = 6.4 +/- 7.6 nmol/l, P < 0.05) and a positive correlation was observed between whole body BMD and IGF-1 in this group. CONCLUSIONS: These results indicate that CCLD limits bone mass gain in children. A reduction in hepatic IGF-1 production might be responsible, at least in part, for the low bone mass of these patients.


Subject(s)
Bone Development/physiology , Cholestasis, Intrahepatic/physiopathology , Absorptiometry, Photon , Adolescent , Alkaline Phosphatase/blood , Amino Acids/urine , Biomarkers/blood , Biomarkers/urine , Body Height , Bone Density/physiology , Bone Remodeling , Case-Control Studies , Child , Child, Preschool , Cholestasis, Intrahepatic/blood , Cholestasis, Intrahepatic/urine , Female , Humans , Infant , Insulin-Like Growth Factor I/analysis , Linear Models , Lumbar Vertebrae , Male , Osteocalcin/blood , Parathyroid Hormone/blood , Vitamin D/analogs & derivatives , Vitamin D/blood
13.
Hepatology ; 43(4): 715-22, 2006 Apr.
Article in English | MEDLINE | ID: mdl-16557543

ABSTRACT

Increased gastrointestinal permeability has been demonstrated in several liver diseases. It may facilitate the absorption of gut-derived endotoxin-stimulating Kupffer cells to release proinflammatory cytokines or other potentially hepatotoxic compounds. We examined gastrointestinal permeability, plasma levels of anti-lipopolysacharides (anti-LPS), and four proinflammatory cytokines in 20 patients with intrahepatic cholestasis of pregnancy (ICP) compared with 22 normal pregnant and 29 non-pregnant women. Urinary excretion of sucrose and the urinary lactulose/mannitol (L/M) ratio after a standard oral load were used to assess gastrointestinal permeability. Anti-LPS (IgA, IgM, and IgG) were measured in peripheral blood by Human EndoCAb test kit; TNF-alpha, IL-1beta, IL-6, and IL-10 by Quantikine HS human immunoassays. Sucrose urinary excretion was similar in the three groups, indicating normal gastric permeability. The urinary L/M ratio was significantly higher in ICP than in the other groups [median (interquartile range): 0.018% (0.011-0.023) in ICP, 0.012% (0.009-0.016) in normal pregnancies, and 0.009% (0.008-0.012) in non-pregnant women, P < .01]. No significant differences were found in anti-LPS or cytokines plasma levels except slightly higher levels of IL-6 in ICP patients than in non-pregnant women (P < .05). Four of five women with abnormal urinary L/M ratio during ICP continued to show abnormalities in tests up to 2 years after delivery. In conclusion, an increased intestinal permeability was detected in ICP patients during and after pregnancy. A "leaky gut" may participate in the pathogenesis of ICP by enhancing the absorption of bacterial endotoxin and the enterohepatic circulation of cholestatic metabolites of sex hormones and bile salts.


Subject(s)
Cholestasis, Intrahepatic/etiology , Intestinal Mucosa/metabolism , Pregnancy Complications/etiology , Adult , Antibodies/blood , Case-Control Studies , Cholestasis, Intrahepatic/blood , Cholestasis, Intrahepatic/immunology , Cholestasis, Intrahepatic/urine , Cytokines/blood , Cytokines/metabolism , Female , Humans , Interleukin-6/blood , Lactulose/urine , Lipopolysaccharides/immunology , Lipopolysaccharides/pharmacology , Mannitol/urine , Monocytes/drug effects , Monocytes/metabolism , Permeability , Pregnancy , Pregnancy Complications/blood , Pregnancy Complications/immunology , Pregnancy Complications/urine , Pregnancy Outcome
14.
Med Sci Monit ; 9(3): MT21-31, 2003 Mar.
Article in English | MEDLINE | ID: mdl-12640349

ABSTRACT

BACKGROUND: It is well known that urine becomes the major route for bile acid excretion in liver diseases and thus we examined bile acid profile in urine obtained from normal children and children having chronic liver diseases using electrospray tandem mass spectrometry (ES/MS/MS). MATERIAL/METHODS: Bile acid were extracted from 5 ml of urine obtained from five healthy children or from twenty patients with various liver diseases including patients with unknown chronic liver diseases, Zellweger syndrome, peroxisomal bifunctional protein deficiency disease, tyrosinema type 1, biliary atresia, and patients with progressive familial intrahepatic cholestasis (PFIC) of undetermined type. Identification and quantification of bile acids were achieved in 5 minutes using electrospray tandem mass spectrometry (ES/MS/MS). RESULTS: Urinary bile acid excretion increased in liver diseases an average of 100 times as compared to control values. There was a specific profile for different liver disease which confirms the pathology of the disease and could be used for its diagnosis. The results also show that the ions used for the diagnosis of oxo-steroid reductase deficiency disease were present in other chronic liver diseases suggesting that these atypical bile acids may not be a result of an inborn error of bile acid metabolism. CONCLUSIONS: The urinary bile acid profile obtained in this study by ES/MS/MS can be of use for the diagnosis of certain chronic liver diseases.


Subject(s)
Bile Acids and Salts/urine , Cholestasis/urine , Metabolism, Inborn Errors/urine , Spectrometry, Mass, Electrospray Ionization/methods , Biliary Atresia/urine , Case-Control Studies , Child , Child, Preschool , Cholestasis, Intrahepatic/genetics , Cholestasis, Intrahepatic/urine , Chronic Disease , Humans , Liver Diseases/diagnosis , Liver Diseases/urine , Peroxisomal Disorders/urine , Reference Values , Syndrome , Tyrosinemias/urine
15.
J Paediatr Child Health ; 37(5): 516-9, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11885722

ABSTRACT

The aim of this study was to evaluate the effects of bile acid treatment and to obtain further information about the pathway of bile acid biosynthesis in a patient with 3beta-hydroxy-delta5-C27-steroid dehydrogenase/isomerase (3beta-HSD) deficiency by gas chromatography-mass spectrometry. Results showed that at 2 months of age, 3beta-hydroxy-5-cholen-24-oic acid (3.0 micromol/mmol Cr, 7.9%) was detected in the urine in essentially the same relative amount as 3beta,7alpha-dihydroxy- and 3beta,7alpha,12alpha-trihydroxy-5-cholen-24-oic acids (3.7 micromol/mmol Cr, 9.8%) during ursodeoxycholic acid treatment combined with prednisolone. As a result, diagnosis was delayed until 18 months of age. One month later with substitution of chenodeoxycholic acid treatment, urinary 3beta,7alpha-dihydroxy- and 3beta,7alpha,12alpha-trihydroxy-5-cholen-24-oic acids decreased significantly, and subsequent improvement of liver dysfunction was accelerated. Chenodeoxycholic acid treatment is useful in 3beta-HSD deficiency. However, in the diagnosis of this disease in early life, it should be noted that the acidic pathway may be the major route for bile acid biosynthesis in the neonatal period. Diagnosis of 3beta-HSD deficiency may have been delayed by administration of ursodeoxycholic acid, resulting in prolonged diagnostic investigation in this child with cholestasis. Further, use of prednisolone may have been contraindicated.


Subject(s)
3-Hydroxysteroid Dehydrogenases/deficiency , Bile Acids and Salts/biosynthesis , Cholestasis, Intrahepatic/enzymology , Bile Acids and Salts/therapeutic use , Cholestasis, Intrahepatic/drug therapy , Cholestasis, Intrahepatic/urine , Humans , Infant , Male , Treatment Outcome
16.
Rev. chil. obstet. ginecol ; 66(4): 306-311, 2001. tab
Article in Spanish | LILACS | ID: lil-310334

ABSTRACT

La colestasia gravídica es una enfermedad de causa desconocida, caracterizada por prurito y anormalidades funcionales hepáticas. Aparece durante la segunda mitad del embarazo y desaparece después del parto. La incidencia en Chile alcanza rangos de 12 a 22 por ciento. La muerte fetal es un evento obstétrico repentino que no se puede predecir por el test convencionales y ultrasonografía (26). De los métodos recientes el nivel de ácidos biliares urinarios parece correlacionarse con el cuadro clínico. Se realizó un estudio prospectivo, caso control, doble ciego en pacientes atendidas en nuestra unidad perinatal durante el transcurso del año 2000 que ingresaron con este diagnóstico. Los datos serán analizados mediante X² cuadrado y se considerará significativo un p > = 0,05. Un total de 5067 partos en el año 2000, 168 cursaron con una colestasia intrahepática del embarazo. El grupo de estudio correspondió a 74 pacientes. Se observa una alta tasa de inducción del parto (41,89 por ciento) una no despreciable tasa de cesárea (33,78 por ciento). Existe un aumento significativo del parto pretérmino y del Apgar menor a 7 al minuto, ambas significativas. No se observa ninguna diferencia significativa en el resultado perinatal de acuerdo al índice de ácidos biliares en orina/creatinemia. El rol de los ácidos biliares en el diagnóstico de la colestasia es poco claro, no parece existir a la luz de los conocimientos actuales un papel en la determinación de ácidos biliares en orina para estimar el riesgo perinatal que ese embarazo conlleva


Subject(s)
Humans , Female , Pregnancy , Adult , Bile Acids and Salts/urine , Cholestasis, Intrahepatic/complications , Pregnancy Complications , Cholestasis, Intrahepatic/diagnosis , Cholestasis, Intrahepatic/urine , Creatinine , Labor, Induced , Pregnancy Complications , Pregnancy, High-Risk , Prospective Studies
18.
Acta Paediatr ; 88(10): 1078-82, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10565453

ABSTRACT

Urinary bile acids and bile alcohols were examined in six infants aged between 1 and 6 mo who had intrahepatic cholestasis. Following extraction, hydrolysis and solvolysis, cholanoids were analysed by gas-liquid chromatography and gas-liquid chromatography-mass spectrometry. The relative ratio of the urinary excretion of bile alcohols to bile acids was very low (0.07-0.22) in three patients with mild to severe cholestasis, whereas the urinary excretion of bile alcohols was 2-4 times greater than that of the total bile acids in three patients with slight cholestasis. The urinary bile alcohol spectrum in infants appears to be quite different from that in adults. Although the major bile alcohol was 27-nor-5beta-cholestane-3alpha,7alpha,12alpha,24 ,25-pentol, comprising more than 50% of total urinary bile alcohols in healthy adults, it accounted for only 35% of total urinary bile alcohols in our patients. In addition, bile alcohols carrying chenodeoxycholic acid type nucleus were detected in our patients by comparison of the retention times and mass spectra with those of authentic standards. The presence of 5beta-cholestane-3alpha,7alpha,24,25-tetrol confirmed for the first time in this study may represent an alternative pathway for chenodeoxycholic acid biosynthesis via a "25-hydroxylation pathway" in early life.


Subject(s)
Bile Acids and Salts/urine , Cholestanols/urine , Cholestasis, Intrahepatic/urine , Adult , Age Factors , Cholestasis, Intrahepatic/diagnosis , Chromatography, Gas , Female , Gas Chromatography-Mass Spectrometry , Humans , Infant , Infant, Newborn , Liver Function Tests , Male , Reference Values , Sensitivity and Specificity
19.
J Hepatol ; 27(2): 346-57, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9288610

ABSTRACT

BACKGROUND/AIMS AND METHODS: The etiology of intrahepatic cholestasis of pregnancy (JCP) is unknown. We have performed comprehensive chromatographic and mass spectrometric analyses of progesterone metabolites and bile acids in serum and urine of six patients in order to characterize changes that might be of importance for the development of the disease. RESULTS: Conjugated bile acids were increased in serum and urine of patients with ICP while the levels of unconjugated bile acids were similar in healthy pregnancies and ICP. Unconjugated and conjugated 7 alpha, 12 alpha-dihydroxy-3-oxo-4-cholenoic acid was excreted in urine both in healthy pregnancies and in ICP, possibly indicating a rate limitation of 3-oxo-delta 4-steroid 5 beta-reductase in pregnancy. The serum levels and urinary excretion of total sulfated progesterone metabolites were increased in ICP while the glucuronides were unchanged or low. Confirming previous results, the fraction of metabolites with 3 alpha-hydroxy-5 alpha(H) configuration was increased. The urinary excretion of 5 alpha-pregnane-3 alpha, 20 alpha-diol 3-sulfate, 20-N-acetylglucosaminide was greatly increased in ICP, as was that of 3 alpha-hydroxy-5 alpha-androstane-17 beta-carboxylic acid, assumed to be a progesterone metabolite. CONCLUSIONS: The combined results of this and previous studies are compatible with a primary change in the reductive metabolism of progesterone in ICP, resulting in increased formation of metabolites with a 3 alpha-hydroxy-5 alpha(H) configuration and a larger fraction of sulfates. There also seems to be a selective defect in the biliary secretion of sulfated metabolites, particularly disulfates.


Subject(s)
Bile Acids and Salts/blood , Bile Acids and Salts/urine , Cholestasis, Intrahepatic/blood , Cholestasis, Intrahepatic/urine , Pregnancy Complications/metabolism , Progesterone/metabolism , Chromatography, Gas , Female , Gas Chromatography-Mass Spectrometry , Humans , Pregnancy , Reference Values , Spectrometry, Mass, Fast Atom Bombardment
20.
Hepatology ; 20(4 Pt 1): 804-12, 1994 Oct.
Article in English | MEDLINE | ID: mdl-7927219

ABSTRACT

The significance of cysteinyl leukotrienes was investigated in patients with liver diseases by measurements of leukotriene E4 and N-acetyl-leukotriene E4 in urine. A marked increase of renal cysteinyl leukotriene excretion was observed in patients with cirrhosis without and with ascites, intrahepatic cholestasis, and obstructive jaundice as compared with healthy subjects (leukotriene E4: means 82, 264, 221 and 142 versus 40 nmol/mol creatinine, respectively; N-acetyl-leukotriene E4: means 25, 64, 61 and 47 versus 13 nmol/mol creatinine, respectively). The urinary concentration of leukotriene E4 was positively correlated with the one of N-acetyl-leukotriene E4 (r = 0.81, p < 0.001). In patients with cirrhosis, the excretion of cysteinyl leukotrienes was strongly increased in patients in Child-Turcotte stage C as compared with those in Child-Turcotte stages A and B. In patients with intrahepatic cholestasis and in those with obstructive jaundice, the excretion of leukotriene E4 plus N-acetyl-leukotriene E4 was positively correlated with total serum bilirubin. In patients with cirrhosis and in those with obstructive jaundice, the cysteinyl leukotrienes in urine were negatively correlated with creatinine clearance. The elevated renal excretion of cysteinyl leukotrienes decreased after biliary drainage in patients with obstructive jaundice. These data support the concept that increased urinary excretion of cysteinyl leukotrienes in patients with cirrhosis is due to a reduced functional liver mass and that in patients with cholestasis it is mainly due to an impaired elimination into the biliary tract that results in a diversion to renal excretion.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Leukotriene E4/urine , Liver Diseases/urine , Adult , Aged , Bilirubin/blood , Cholestasis/surgery , Cholestasis/urine , Cholestasis, Intrahepatic/urine , Chromatography, High Pressure Liquid , Creatinine/urine , Cysteine , Drainage , Female , Humans , Leukotriene E4/analogs & derivatives , Liver Cirrhosis/urine , Liver Diseases/blood , Male , Middle Aged , Radioimmunoassay , Regression Analysis
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