1.
Bioorg Med Chem Lett
; 12(11): 1485-8, 2002 Jun 03.
Article
in English
| MEDLINE
| ID: mdl-12031325
ABSTRACT
For monitoring low-density lipoprotein receptors (LDLr) in tumors and in livers of patients with familial hypercholesterolemia (FH) treated with gene therapy, a series of tricarbocyanine cholesteryl laurates were synthesized with the cholesteryl laurate moiety serving as the lipid-chelating anchor for low-density lipoprotein (LDL). One of these conjugates, TCL17, was successfully used to label LDL to give a new NIRF, TCL17-LDL. Ex vivo biological studies on an LDLr overexpressing tumor model, human hepatoblastoma G(2) (HepG(2)), confirmed that this NIRF were internalized selectively by the tumor and detected with high sensitivity by a low-temperature 3-D redox scanner.
Subject(s)
Cholesterol Esters/metabolism , Cholesterol, LDL/metabolism , Fluorescent Dyes/metabolism , Hepatoblastoma/metabolism , Hyperlipoproteinemia Type II/metabolism , Laurates/metabolism , Liver Neoplasms/metabolism , Receptors, LDL/metabolism , Animals , Carbocyanines/chemical synthesis , Carbocyanines/chemistry , Carbocyanines/pharmacokinetics , Cholesterol Esters/chemistry , Cholesterol Esters/pharmacokinetics , Cholesterol, LDL/chemical synthesis , Cholesterol, LDL/chemistry , Cholesterol, LDL/pharmacokinetics , Fluorescent Dyes/chemical synthesis , Fluorescent Dyes/chemistry , Fluorescent Dyes/pharmacokinetics , Genetic Therapy , Hepatoblastoma/drug therapy , Humans , Hyperlipoproteinemia Type II/therapy , Imaging, Three-Dimensional , Laurates/chemical synthesis , Laurates/chemistry , Laurates/pharmacokinetics , Liver Neoplasms/therapy , Mice , Spectroscopy, Near-Infrared
2.
Med. interna (Caracas)
; 4(1/2): 3-7, ene.-jun. 1988.
Article
in Spanish
| LILACS
| ID: lil-97925