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1.
Nucleic Acids Res ; 45(16): 9388-9397, 2017 Sep 19.
Article in English | MEDLINE | ID: mdl-28934466

ABSTRACT

Sister chromatid cohesion, mediated by cohesin complex and established by the acetyltransferases Esco1 and Esco2, is essential for faithful chromosome segregation. Mutations in Esco2 cause Roberts syndrome, a developmental disease characterized by severe prenatal retardation as well as limb and facial abnormalities. However, its exact roles during oocyte meiosis have not clearly defined. Here, we report that Esco2 localizes to the chromosomes during oocyte meiotic maturation. Depletion of Esco2 by morpholino microinjection leads to the precocious polar body extrusion, the escape of metaphase I arrest induced by nocodazole treatment and the loss of BubR1 from kinetochores, indicative of inactivated SAC. Furthermore, depletion of Esco2 causes a severely impaired spindle assembly and chromosome alignment, accompanied by the remarkably elevated incidence of defective kinetochore-microtubule attachments which consequently lead to the generation of aneuploid eggs. Notably, we find that the involvement of Esco2 in SAC and kinetochore functions is mediated by its binding to histone H4 and acetylation of H4K16 both in vivo and in vitro. Thus, our data assign a novel meiotic function to Esco2 beyond its role in the cohesion establishment during mouse oocyte meiosis.


Subject(s)
Acetyltransferases/metabolism , Histones/metabolism , Kinetochores/metabolism , M Phase Cell Cycle Checkpoints/genetics , Meiosis/genetics , Oocytes/enzymology , Acetylation , Acetyltransferases/physiology , Aneuploidy , Animals , Chromosomes, Mammalian/enzymology , Female , Histones/chemistry , Lysine/metabolism , Mice, Inbred ICR , Spindle Apparatus/metabolism
2.
Methods ; 38(1): 52-9, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16343931

ABSTRACT

The centromere or kinetochore functions in both chromosome movement and in regulation of progression through mitosis. It appears likely that the signaling pathways involved are keenly dependent on solid phase cytoskeletal and karyoskeletal scaffolds that may mediate important physical signals such as tension. Understanding these pathways will be greatly aided by reconstructing the signaling in lysed cell models. Here we present approaches to the in vitro study of signaling pathways in mitotic cells, particularly those involved in protein phosphorylation changes at kinetochores that may control cell cycle progression in M phase.


Subject(s)
Kinetochores/enzymology , Phosphoric Monoester Hydrolases/metabolism , Phosphotransferases/metabolism , Animals , Cell Line , Centromere/chemistry , Centromere/enzymology , Chromosomes, Mammalian/enzymology , Cytogenetics/methods , Electrophoresis, Polyacrylamide Gel , Kinetochores/chemistry , Microscopy, Fluorescence , Mitosis/physiology , Models, Biological , Phosphorylation , Potoroidae , Signal Transduction
3.
Arch Inst Pasteur Tunis ; 78(1-4): 33-40, 2001.
Article in French | MEDLINE | ID: mdl-14658237

ABSTRACT

The Robertsonian phenomenon in house mice (Mus musculus domesticus) from Tunisia consists in the presence of only one 22-chromosome Robertsonian race (22Rb) carrying the maximum number of fusions observed until now. The 22Rb populations exclusively occupy urban centers in the Eastern-Central region of Tunisia where standard population with 40-all acrocentric chromosomes (40Std) occur in surrounding neighborhoods and rural environments. In addition to the habitat partition, allozyme and mitochondrial DNA analyses showed that the 22Rb populations were genetically differentiated from the 40Std ones. This differentiation mostly stemmed from an important decrease in genetic variability in the 22Rb populations from the Sahel towns. The extent of morphological, ecological and genetical divergence observed between these chromosomal races in Tunisia is in agreement with the predictions of the chromosomal speciation model of White which advocates that karyotypic differentiation between taxa can lead to their reproductive isolation and independent evolution. Such a process is verified if the Rb process in Tunisia results from local differentiation which is supported by both the genetic and morphological data. However, the hypothesis of an origin by introduction of these mice from another region of Tunisia or from another country cannot be totally dismissed. In this study, an allozymic analysis of mice (22Rb and 40Std) from the geographically distant city of Kairouan was performed. Results showed that 22Rb and 40Std mice from Kairouan shared the same high degree of variability, and were not genetically differentiated. This contrasts with the results registered in the two chromosomal races in the Sahel towns. Such data argue in favor of a local differentiation of the Robertsonian process in Tunisia and suggest that the decrease in variability of the structural nuclear genes in the Sahel 22Rb populations can be related to an introduction from Kairouan into a Sahel locality resulting in a founder effect or followed by a severe bottleneck prior to its dispersion throughout the Sahel region.


Subject(s)
Chromosome Aberrations/veterinary , Chromosomes, Mammalian/genetics , Mice/genetics , Animals , Chromosomes, Mammalian/enzymology , Cytogenetic Analysis , DNA, Mitochondrial/genetics , Environment , Founder Effect , Genetic Variation/genetics , Genetics, Population , Homozygote , Karyotyping , Models, Genetic , Phylogeny , Translocation, Genetic/genetics , Tunisia/epidemiology , Urban Population
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