Phylogenetic diversity and metabolic potential of microbiome of natural healing clay from Chamliyal (J&K).

Clay therapy for skin disease treatment is an ancient practice popular worldwide as a cheap alternative to pharmaceutical products. Effectiveness of clay against skin problems has been linked to its mineral composition and to microbial activity. The clay-water paste of a holy shrine Chamliyal in the Jammu region of J&K, India is used as an ointment to treat different skin disorders particularly psoriasis. Using the 16 SrDNA amplicon pyrosequencing and whole-metagenome direct shotgun Illumina sequencing, microbial phylogeny and potential metabolic functions were catalogued for Chamliyal's clay. Microbial diversity profile of the Chamliyal's clay is similar to other medicinal clays, particularly Dead Sea; there is some uniqueness as well. Although Proteobacteria, Actinomycetes and Firmicutes are common inhabitants of all the clay types, sulphur- and iron-reducing bacteria like Deferribacterales are particular to clays used for skin healing. In the present study it is proposed that healing properties of clay may be due to the microbes and microbial genes associated with metabolism of minerals like iron and sulphur, that lead to mineral acquisition in the Chamliyal's clay.

Disparities in correlating microstructural to nanostructural preservation of dinosaur femoral bones.

Osteohistological researches on dinosaurs are well documented, but descriptions of direct correlations between the bone microstructure and corresponding nanostructure are currently lacking. By applying correlative microscopy, we aimed to verify that well-preserved osteohistological features correlate with pristine fossil bone nanostructures from the femoral bones of Koreanosaurus boseongensis. The quality of nanostructural preservation was evaluated based on the preferred orientation level of apatite crystals obtained from selected area electron diffraction (SAED) patterns and by measuring the "arcs" from the {100} and {002} diffraction rings. Unlike our expectations, our results revealed that well-preserved microstructures do not guarantee pristine nanostructures and vice versa. Structural preservation of bone from macro- to nanoscale primarily depends on original bioapatite density, and subsequent taphonomical factors such as effects from burial, pressure, influx of external elements and the rate of diagenetic alteration of apatite crystals. Our findings suggest that the efficient application of SAED analysis opens the opportunity for comprehensive nanostructural investigations of bone.

The effect of natural and organophilic palygorskite on skin wound healing in rats

In view of growing interest in natural treatments, clays would appear to be a good alternative for speeding up the healing process during the treatment of wounds. Of the various clays, palygorskite, a clay from the Brazilian State of Piauí, composed of silicon and aluminum, has shown itself to be pharmaceutically useful as a healing agent. The aim of this article is to evaluate the effect on the healing of wounds of Piauí palygorskite, both in its natural state and when organophilic, by way of comparative analysis of macroscopic and histological tests on skin wounds in adult male and female two-month-old Wistar rats. To this end, a circular trichotomy of the dorsal cornus of the rats was carried out to confirm the effects of treatments involving 0.9% saline solution, collagenase, natural palygorskite, organophilic palygorskite with cetyltrimethylammonium chloride, and organophilic palygorskite with alkyldimethylbenzylammonium chloride. The testing of all the clays involved microbiological evaluation using the depth of plaque and surface striation methods, along with post-treatment macroscopic analysis of skin wounds by way of organoleptics, pachymetry and histological analysis. Microbiological evaluation revealed the need for sterilization of the clay prior to incorporation in the pharmaceutical form. Macroscopic analysis suggests that healing of the wounded area occurred, and histological analysis showed the beneficial effect of the topical use of clay material. Our data suggest that palygorskite may be more powerful than other healing agents, although, on completing treatment, all the animals studied showed the same degree of tissue repair.

Devido ao crescente interesse da população pelos tratamentos naturais, as argilas representam uma boa alternativa para a aceleração da cicatrização durante o tratamento de feridas. Dentre as argilas, a paligorsquita, uma argila piauiense, devido à sua composição com silício e alumínio, demonstra certa aplicabilidade farmacêutica como agente cicatrizante. O objetivo deste artigo é avaliar a ação cicatrizante da paligorsquita piauiense em sua forma natural e organofilizadas por meio da análise comparativa da macroscopia e dos exames histológicos em feridas cutâneas de ratos machos e fêmeas Wistar adultos com 2 meses de idade. Para tanto, foram realizadas tricotomia circular no corno dorsal dos ratos para verificar os efeitos dos tratamentos realizados com solução salina 0,9%, colagenase, paligorsquita natural, paligorsquita organofilizada por cloreto de cetiltrimetilamônio e paligorsquita organofilizada por cloreto de alquildimetilbenzilamônio. Para todas as argilas testadas foi feita a avaliação microbiológica pelo método de plaqueamento em profundidade e do método de estrias em superfície, bem como foi realizada após tratamento, a análise macroscópica das feridas cutâneas por meio organoléptico, medição em paquímetro e análise histológica. Por meio da avaliação microbiológica foi detectada a necessidade de esterilização da argila para posterior incorporação na forma farmacêutica. A análise macroscópica sugere que houve cicatrização da área lesionada, bem como a análise histológica demonstrou efeito benéfico após o uso tópico do material argiloso. Nossos dados sugerem que a paligorsquita pode exercer um maior efeito cicatrizante em relação aos demais tratamentos, embora após o término deste tratamento, todos os animais analisados apresentaram a mesma reparação tecidual.

Avaliação de alguns atributos físicos de um latossolo vermelho-amarelo sob diferentes coberturas vegetais / Evaluation of some physical attributes of an oxisol under different crop cover

Os atributos do solo são dinâmicos e estão sujeitos a alterações de acordo com o uso e manejo adotado, sendo sua determinação importante para melhor utilização agrícola dos solos. Desta forma, objetivou-se avaliar o comportamento de alguns atributos físicos (composição granulométrica, argila dispersa em água e grau de floculação) de um Latossolo Vermelho-Amarelo sobre diferentes usos, a saber: cultivado com duas espécies de leguminosas e sob vegetação espontânea, em área anteriormente cultivada com horticultura, por aproximadamente 10 anos, sob intensa mecanização agrícola e aplicação de corretivos e fertilizantes. O experimento foi instalado no delineamento em blocos casualizados, sendo conduzido em esquema fatorial 2 x 3, sendo duas profundidades (0 a 20 e 20 a 40 cm) e três coberturas vegetais (duas leguminosas e uma vegetação espontânea) com 4 repetições cada. Os dados das avaliações foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Observou-se que a agitação lenta proporciona uma melhor dispersão física quando comparada com a agitação rápida, demonstrando maior acurácia e precisão. Os valores de argila dispersa em água são maiores na profundidade de 20-40 cm. O solo cultivado com mucuna preta, na profundidade de 20-40 cm, apresenta um maior valor de argila dispersa em água em relação às demais coberturas vegetais e o solo sob vegetação espontânea maior grau de floculação nesta profundidade.

Soil attributes are dynamic and are subject to change according to the use and management adopted, is important for determining its best use agricultural soil. Thus, it was aimed to evaluate some physical attributes (particle size, water dispersible clay and flocculation) of an Oxisol on different uses, namely: cultivated with two species of legumes and natural vegetation, in an area previously cultivated with vegetables, for about 10 years, under intense agricultural mechanization and application of lime and fertilizer. The experiment was conducted in a randomized block design, being conducted in factorial 2 x 3, two depths (0 to 20 and 20 to 40 cm) and three cover crops, with four replications. Data were submitted to analysis of variance and averages compared by Tukey test at 5% probability. It was Avaliação de alguns atributos... SANTOS, L. N. S. et al. Biosci. J., Uberlândia, v. 26, n. 6, p. 940-947, Nov./Dec. 2010 946 observed that the slow agitation provides better physical dispersion when compared with the fast agitation, showing greater accuracy and precision. The values of clay dispersed in water are larger at a depth of 20-40 cm. The soil under Mucuna pruriens L., in depth of 20-40 cm, has a higher value of water dispersible clay in water compared with to other cover crops and the soil under natural vegetation greater degree of flocculation this depth

A geographic information system (GIS) analysis for trace metal assessment of sediments in the Gulf of Paria, Trinidad

The Gulf of Paria is a semi-enclosed shallow basin with increasing coastal development activities along Trinidad's west coast. Sediments present a host for trace metal pollutants from overlying waters, therefore determination of their content is critical in evaluating and detecting sources of marine pollution. This paper presents a Geographic Information System (GIS) analysis of geochemical assessment for trace metals in coastal sediments of the Gulf of Paria. This GIS approach facilitates interpretation of the spatial relationships among key environmental processes. The GIS development involves the integration of spatial and attribute data pertaining to bathymetry, current systems, topography, rivers, land use/land cover and coastal sediments. It employs spatial interpolation and retrieval operations to analyze the total trace metal concentrations of aluminum, copper and lead in the sediments and the clay-enriched sediments, to determine whether they are related to sediment type or are affected by the discharge from anthropogenic sources. Spatial distribution modeling of element concentrations are produced to indicate contamination plumes from possible anthropogenic sources such as rivers entering the Gulf of Paria, and to reveal potential hot spots and dispersion patterns. A direct spatial correlation between clay-enriched sediments and high concentrations of aluminum and lead is detected, however regions of high concentrations of copper and lead indicate a relationship to anthropogenic sources. The effectiveness of GIS for visualization, spatial query and overlay of geochemical analysis is demonstrated.