ABSTRACT
Long-term, low-level, BrdUrd infusion identifies two subpopulations of GM-CFCc with quite dissimilar sensitivities to 313 nm light. The responses of these two GM-CFCc subpopulations to hydroxyurea indicate that both are rapidly proliferating at the time of the assay. However, the absolute UV-light sensitivity of the S-phase components and the effects of increasing BrdUrd concentration indicate that the two GM-CFCc subpopulations passed through the previous cell cycle at widely disparate rates. Further, those GM-CFCc originating from a parental cell with a slow turnover are associated with a lower buoyant density than those GM-CFCc that have been in rapid cycle for at least two generations. These results indicate that the resistance to 313 nm-light irradiation, shown by S-phase cells in the first cell cycle of the BrdUrd labeling, may provide evidence of the proliferative history of the cell being assayed.
Subject(s)
Bromodeoxyuridine/pharmacology , Colony-Forming Units Assay/classification , Animals , Bromodeoxycytidine/pharmacology , Cell Cycle , Centrifugation, Density Gradient , Female , Hydroxyurea/pharmacology , Interphase , Kinetics , Light , MiceABSTRACT
Although 12-hours postmortem murine bone marrow cells exhibit extensive degeneration, these cells when infused into irradiated mice produce erythrocytic, granulocytic, megakarocytic and mixed colonies in their spleen. These observations clearly demonstrate the presence of pluripotent hemopoietic stem cells and their proliferative capability in 12-hours postmortem, murine bone marrow. These observations suggest that cadaveric bone marrow transplantation may be possible in patients with hematologic disorders.
