Surface-Engineered Contact Lens as an Advanced Theranostic Platform for Modulation and Detection of Viral Infection.

We have demonstrated an entirely new concept of a wearable theranostic device in the form of a contact lens (theranostic lens) with a dual-functional hybrid surface to modulate and detect a pathogenic attack, using a the corneal HSV serotype-1 (HSV-1) model. The theranostic lenses were constructed using a facile layer-by-layer surface engineering technique, keeping the theranostic lenses with good surface wettability, optically transparency, and nontoxic toward human corneal epithelial cells. The theranostic lenses were used to capture and concentrate inflammatory cytokines such as interleukin-1α (IL-1α), which is upregulated during HSV-1 reactivation, for sensitive, noninvasive diagnostics. The theranostic lens also incorporated an antiviral coating to serve as a first line of defense to protect patients against disease. Our strategy tackles major problems in tear diagnostics that are mainly associated with the sampling of a relatively small volume of fluid and the low concentration of biomarkers. The theranostic lenses show effective anti-HSV-1 activity and good analytical performance for the detection of IL-1α, with a limit of detection of 1.43 pg mL(-1) and a wide linear range covering the clinically relevant region. This work offers a new paradigm for "wearable" noninvasive healthcare devices combining "diagnosis" and "protection" against disease, while supporting patient compliance. We believe that this approach holds immense promise as a next-generation point-of-care and decentralized diagnostic/theranostic platform for a range of biomarkers.

Some secrets are revealed: parasitic keratitis amoebae as vectors of the scarcely described pandoraviruses to humans.

In this article, the results of a long effort to derive valuable phylogenetic data about an extraordinary spore-like infectious particle (endocytobiont) within host amoebae (Acanthamoeba sp.) recently isolated from the contact lens and the inflamed eye of a patient with keratitis are presented. The development of these endocytobionts has already been demonstrated with electron microscopic photo sequences, leading to a relevant model of its development presented here. The molecular biological investigation following the discovery of two other Pandoravirus species within aquatic sediments in 2013 led to the taxonomic affiliation of our endocytobiont with the genus Pandoravirus. A range of endocytobionts (intracellular biofilms) have been found in recent years, among which are several viruses which obligatorily proliferate within free-living amoebae. In human medicine, foreign objects which are placed in or on humans cause problems with microorganisms in biofilms. Contact lenses are especially important, because they are known as a source of a rapid formation of biofilm. These were the first Pandoraviruses described, and because this is additionally the first documented association with humans, we have clearly demonstrated how easily such (viral) endocytobionts can be transferred to humans. This case counts as an example of parasites acting as vectors of phylogenetically different microorganisms especially when living sympatric within their biocoenosis of biofilms. As the third part of the "Pandoravirus trilogy", it finally reveals the phylogenetic nature of these "extraordinary endocytobionts" within Acanthamoebae.

Herpes Simplex epithelial keratitis associated with daily disposable contact lens wear.

PURPOSE: To report a case of epithelial Herpes Simplex keratitis in a patient wearing daily disposable contact lenses. METHOD: Case report. RESULTS: A 30-year-old female contact lens wearer presented to the emergency clinic with a painful, red left eye associated with an acute reduction of vision over 48 h. On examination, confluent dendritic ulcers were present on the cornea. Neither pertinent ocular nor medical history was obtained to explain such a dramatic clinical presentation. CONCLUSION: Contact lens wear was the only risk factor identified, perhaps resulting in deviation of the immune response at the ocular surface, with consequent extensive dendritic ulceration.

The disinfection of contact lenses contaminated with adenovirus.

PURPOSE: We compared the efficacy of different contact lens disinfection systems to eliminate adenovirus. METHODS: Laboratory study evaluating the elimination of adenoviral ocular isolates by contact lens disinfection systems. Hard (gas permeable) and soft contact lenses were contaminated with adenovirus serotypes 8 and 19, and then they were disinfected with chemical, hydrogen peroxide, and heat sterilization systems. The survival of the adenovirus was determined by the shell vial technique. RESULTS: Adenovirus survived chemical and hydrogen peroxide disinfection but not heat sterilization. CONCLUSION: Because heat sterilization is not readily available to sterilize adenovirus contaminated contact lenses, it may be prudent for patients with adenoviral keratoconjunctivitis to dispose of unclean contact lenses.