ABSTRACT
Detailed knowledge of the anatomy of human placenta vessels is clinically essential and requires the use of many different anatomical and histological techniques. One of the interesting methods of visualising vessels is the corrosion technique. It enables spatial visualisation of the vascular network of the analysed organ. The authors present a developed, own method of preparing the corrosive preparations from human placenta. They underline the advantages and disadvantages of this technique. They describe solutions aimed at reducing the costs of the process. They show that corrosion technology enables relatively fast and inexpensive visualisation of arterial and venous vessels of the human placenta.
Subject(s)
Corrosion Casting/methods , Corrosion Casting/standards , Placenta/blood supply , Adult , Arteries/anatomy & histology , Female , Gestational Age , Humans , Infant, Newborn , Male , Maternal Age , Parity , Pregnancy , Umbilical Cord/anatomy & histology , Veins/anatomy & histologyABSTRACT
PURPOSE: To develop and optimize a system for treating cerebral berry aneurysm using a new polymer based liquid embolic agent. MATERIALS AND METHODS: Using a variety of human corrosion casting aneurysm replicas placed in a circuit of pulsatile flowing blood, techniques were developed to optimize aneurysm filling and recreate good blood flow dynamics. As part of this effort, both experienced physicians and lay personnel were asked to treat these replicas to discover common failure modes. Additional surgically created aneurysms in dog carotid arteries were treated by experienced physicians. RESULTS: Physicians experienced in the use of microcatheters and endovascular techniques learned to use the device effectively after, on average, five aneurysm treatments. In the dog aneurysms, all were treated to complete filling.
Subject(s)
Catheterization/methods , Corrosion Casting/methods , Cyanoacrylates/administration & dosage , Intracranial Aneurysm/surgery , Tissue Adhesives/administration & dosage , Animals , Catheterization/standards , Clinical Laboratory Techniques/methods , Clinical Laboratory Techniques/standards , Corrosion Casting/standards , Disease Models, Animal , Dogs , Humans , Intracranial Aneurysm/classification , Intracranial Aneurysm/pathology , Sheep , SwineABSTRACT
Scanning electron microscopy (SEM) of vascular corrosion casts (microvascular corrosion casting/SEM method) is a standard method, which allows three-dimensional visualization with good resolution of the normal and abnormal microvessels, including the capillaries of various organs and tissues. SEM of vascular corrosion casts can obtain qualitative as well as quantitative informations important to anatomists, pathologists and clinicians. Considering these, the history, the advantages and the main steps of this technique including general morphological characteristics of vascular casts observed in SEM are reviewed in this paper. Corrosion casts done by the author representing the microvascular organization of the rat liver and kidney observed in SEM are, also, presented.
Subject(s)
Corrosion Casting/methods , Corrosion Casting/standards , Microcirculation/ultrastructure , Microscopy, Electron, Scanning/methods , Animals , Caustics/chemistry , Caustics/pharmacology , Humans , Polymers/chemistry , Polymers/metabolismABSTRACT
Las proporciones anatómicas por corroción obtenidas utilizando solución de celulcido en acetoma, permiten conseguir una reproducción exacta de la forma, cituación, calibre, trayecto y modo de distribución de los vasos arteriales y venosos inyectados, hasta en sus más finos ramúsculos, con una objetividad dificilmente alcanzada de otro modo
