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1.
J Obstet Gynaecol ; 40(2): 222-227, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31390918

ABSTRACT

Our study assessed the safety and clinical outcomes of hysteropexy with a single-incision mesh associated with a modified culdoplasty, for the surgical management or prevention of enterocele, in women with pelvic organ prolapse (POP). We carried out a 1-year prospective single-cohort study, including 51 women with symptomatic, multi-compartmental POP. Anatomical outcome was assessed with a POP-Q system and the subjective outcomes were assessed using ICSQ-SF and PGI-I. One-year follow-up data were available for 48 out of 51 patients. The POP-Q cure rate was 91%, 83% of patients were satisfied or very satisfied (PGI-I ≤ 2). No major complications occurred; the most common minor complications were mesh erosion (6%) and pelvic pain (8%). Lower urinary tract dysfunctions arose in 16% of the patients. Anatomical prolapse recurrence (POP-Q stage ≥2) in anterior or apical compartments occurred in four patients (8%). No case of de novo prolapse occurred in the posterior compartment. None of the patients required further surgery for recurrent prolapse. This standardised procedure provided satisfactory 'restitutio ad integrum' of the vaginal anatomy and symptom relief.Impact statementWhat is already known on this subject? The post-surgical evidence of de novo prolapse in untreated compartments is well-known, especially in prosthetic surgery. The insertion of polypropylene mesh causes a vigorous support, consequently the forces on the pelvic floor are transmitted to the least consolidated vaginal compartment. A lack of simultaneous repair of all the segments involved in the POP increases the risk of surgical recurrence even in those areas that did not appear to be pre-operatively affected by the uterine descensus.What the results of this study add? Our prospective study showed that hysteropexy with a single-incision vaginal support system plus a modified culdoplasty was able to prevent the enterocele and the occurrence of prolapse in the posterior compartment, by closing the Douglas pouch and restoring the connection of the rectovaginal septum with the apical support.What the implications are of these findings for clinical practice and/or further research? This study may be relevant for clinicians in selecting the technique for pelvic floor surgery, and it may be of interest for researchers investigating the reasons for de novo occurrence of posterior segment prolapse.


Subject(s)
Culdoscopy/methods , Hernia/prevention & control , Hysteroscopy/methods , Pelvic Organ Prolapse/surgery , Plastic Surgery Procedures/methods , Postoperative Complications/prevention & control , Aged , Female , Humans , Middle Aged , Patient Satisfaction , Prospective Studies , Recurrence , Suburethral Slings , Treatment Outcome , Vagina/surgery
2.
Biomed Res Int ; 2019: 8047924, 2019.
Article in English | MEDLINE | ID: mdl-31236413

ABSTRACT

BACKGROUND: In gynecological surgery, one particular area of concern after hysterectomy is the risk of developing an enterocele or vaginal apical prolapse. The aims of this study were to evaluate the safety and efficacy of prophylactic McCall culdoplasty (MC) performed during mini-total laparoscopic hysterectomy (mini-TLH), as well as to compare the differences in apical support, total vaginal length (TVL), and sexual function at one and two years postoperatively. METHODS: Data were retrospectively reviewed for all women who underwent mini-TLH and mini-TLH + MC at a tertiary care center between August 2012 and January 2016 were from the hospital database. There were 18 women who underwent mini-TLH + MC and were considered as the study group, while 20 women who were treated with mini-TLH were considered as the control group. The primary outcome measures were the differences in apical support and TVL and impact on sexual function. RESULTS: After mini-TLH + MC, the apical vaginal support difference was improved by 2.2 cm. The mean difference in C point was 1.03 cm in the mini-TLH group, which was not significant at two years after the operation. The vaginal vault descent at two years after operation was more prominent in the mini-TLH group than the mini-TLH + MC groups. Apical support changes at two years after the operation were more prominent in the mini-TLH group (0.5 ± 0.6 cm) than the mini-TLH + MC group (1.9 ± 1.2 cm). CONCLUSION: Prophylactic MC by a vaginal approach during mini-TLH is safe, satisfactory, and efficient for apical support without severe morbidity.


Subject(s)
Culdoscopy/methods , Hysterectomy, Vaginal/adverse effects , Postoperative Complications/prevention & control , Vagina/surgery , Female , Humans , Laparoscopy/adverse effects , Middle Aged , Postoperative Complications/physiopathology , Uterine Prolapse/etiology , Uterine Prolapse/physiopathology , Vagina/physiopathology
3.
Eur J Obstet Gynecol Reprod Biol ; 223: 113-118, 2018 Apr.
Article in English | MEDLINE | ID: mdl-29518641

ABSTRACT

OBJECTIVES: The aim of this study was to evaluate the effectiveness of modified McCall culdoplasty or Shull suspension in preventing vaginal vault prolapse after vaginal hysterectomy and the long-term impact on quality of life and sexual function. STUDY DESIGN: Retrospective analysis in 414 patients underwent vaginal hysterectomy for pelvic organ prolapse (POP) and vaginal suspension through modified McCall culdoplasty (group A) or Shull suspension (group B) was evaluated. Clinical features and concomitant surgical procedures were assessed. Surgical data and perioperative and postoperative complications have also been analyzed. Clinical characteristics, urinary symptoms, POP-Q score classification, Quality of Life and Sexual Function were evaluated at baseline and at median follow up with P-QoL, ICIQ-UI-SF, PISQ-12, FSFI, FSDS questionnaires. RESULTS: The median follow up was 8.9 year (5.1-14.2 years). 200 women in group A and 214 in group B were evaluated. Vaginal vault prolapse occurred in 2 patients in group A and in 2 patients in group B. POP-Q score for all compartments showed a significant (p < 0.001) decrease for both groups without significant differences between the 2 groups. The total vaginal length (TVL) was reduced in greater proportion in McCall group (p < 0.001). P-QoL and ICIQ-UI-SF questionnaires documented an improvement for both groups (p < 0.001). The number of patients who regularly practice sexual activity increased in both groups, but patients in group B experienced a better quality of sexual life evaluated with PISQ-12 and FSFI. CONCLUSIONS: Both surgical techniques showed effectiveness and safety in preventing vaginal vault prolapse in women who underwent vaginal hysterectomy, with a significant improvement in quality of life and sexuality. Shull technique demonstrated greater improvement in sexual function.


Subject(s)
Hysterectomy, Vaginal/methods , Pelvic Organ Prolapse/prevention & control , Pelvic Organ Prolapse/surgery , Quality of Life , Aged , Culdoscopy/methods , Female , Humans , Intraoperative Complications/epidemiology , Middle Aged , Postoperative Complications/epidemiology , Retrospective Studies , Sexual Behavior , Surveys and Questionnaires , Treatment Outcome , Vagina/pathology , Vagina/surgery
4.
Int Urogynecol J ; 28(1): 65-71, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27048368

ABSTRACT

INTRODUCTION AND HYPOTHESIS: Uterosacral ligament suspension at the time of primary prolapse repair represents a well-established surgical option. Our aim was to compare the effectiveness, complications rate, and functional results of modified McCall culdoplasty and Shull suspension. METHODS: Patients who underwent vaginal hysterectomy and cuff suspension for pelvic organ prolapse were retrospectively analyzed. McCall culdoplasty (group A) or Shull suspension (group B) were performed according to surgeon choice based on age and sexual activity. Perioperative data, objective, and subjective cure rate were noted. RESULTS: A total of 339 patients (215 in group A and 124 in group B) completed follow-up. Operating time and blood loss were slightly higher in group B. The complications rate was similar in the two groups. Anatomical outcomes in terms of recurrence and reoperation rate did not show any statistically significant differences. POP-Q items analysis revealed only a different total vaginal length between groups (8 mm longer in group B). Functional outcomes were similar in the two groups as was patient satisfaction. CONCLUSION: Both uterosacral ligament suspension procedures were shown to be safe and effective. There were no clinically significant differences with regard to surgical data, complications, anatomical, functional, and subjective outcomes between modified McCall culdoplasty and Shull suspension.


Subject(s)
Culdoscopy/methods , Hysterectomy, Vaginal/methods , Pelvic Organ Prolapse/surgery , Plastic Surgery Procedures/methods , Vagina/surgery , Aged , Blood Loss, Surgical/statistics & numerical data , Female , Follow-Up Studies , Humans , Middle Aged , Operative Time , Retrospective Studies , Treatment Outcome
6.
Minerva Ginecol ; 68(4): 418-22, 2016 Aug.
Article in English | MEDLINE | ID: mdl-25900769

ABSTRACT

BACKGROUND: Culdotomy is a practically exclusive appanage of gynaecological surgeons. Today the transvaginal access to pelvis and abdominal cavity is also utilized for non-gynecological surgery. METHODS: The authors report the complications and compliance of 326 culdotomies performed in the Simple Maternity Unit of Asiago Hospital (Asiago, Vicenza, Italy). The casuistry includes 18 adnexetomies, 90 ovarian cysts and fibroids enucleations and/or resections, 23 paraovarian cysts removals, 59 myomectomies, 120 sterilizations, 11 salpingectomies, 1 adesiolysis, 2 ovarian biopsies and 1 abscess drainage. RESULTS: Couldtomy showed to be a procedure with good results in terms of 18 grade 1 and 1 grade IV (5.8%) of Clavien-Dindo grading system for classification of surgical complications, the incidence of conversions in laparotomies, laparoscopies and vaginal hysterectomy (3.1%), the average hospital postoperatory admission (25 hours), low cost of culdotomy compared to laparotomic and laparoscopic surgery; absence of dispareunia and/or other discomforts at long term. CONCLUSIONS: Culdotomy is a simple, proper and alternative route, especially when morcellation is unavoidable.


Subject(s)
Culdoscopy/methods , Genital Diseases, Female/surgery , Morcellation/methods , Postoperative Complications/epidemiology , Culdoscopy/adverse effects , Culdoscopy/economics , Female , Genital Diseases, Female/pathology , Humans , Hysterectomy, Vaginal/methods , Italy , Laparoscopy/economics , Laparoscopy/methods , Laparotomy/economics , Laparotomy/methods
7.
Eur J Gynaecol Oncol ; 36(3): 335-8, 2015.
Article in English | MEDLINE | ID: mdl-26189265

ABSTRACT

PURPOSE OF INVESTIGATION: To report and explore the effectiveness of the endoscopic surgery combining cisplatin, etoposide, and bleomycin (PEB) chemotherapy in treating vaginal yolk-sac tumor. CASE REPORT: The clinical case of vaginal yolk-sac tumor in a young girl was analyzed. Hysteroscopy equipment was used to inspect and remove the majority of the tumor tissue, followed by six cycles of PEB chemotherapy. Hysteroscopy equipment was used again to exam the vagina and remove of the tumor pedicle for biopsy, which revealed no tumor cells after two cycles of chemotherapy. The patient remained disease free on follow up for a year. CONCLUSION: The use hysteroscopy equipment to exam vagina can diagnose early vaginal yolk-sac tumor and cytoreductive surgery which can preserve fertility can then be performed. Cytoreductive surgery may also be able to reduce the cycles of the chemotherapy to reduce the side effects and the risks of chemotherapy.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Culdoscopy/methods , Endodermal Sinus Tumor/therapy , Vagina/surgery , Vaginal Neoplasms/therapy , Bleomycin/therapeutic use , Cisplatin/therapeutic use , Endodermal Sinus Tumor/pathology , Etoposide/therapeutic use , Female , Humans , Infant , Vagina/pathology , Vaginal Neoplasms/pathology
8.
Clin Obstet Gynecol ; 55(2): 387-94, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22510619

ABSTRACT

The declining death rate from ectopic pregnancy over the last 30 years has resulted not from improvements in operative technique, anesthesia, or blood banking but primarily due to more rapid diagnosis that allows for earlier treatment. Algorithms have been developed to eliminate delays in ordering the various diagnostic tests in the appropriate order. Diagnostic tests include serum human chorionic gonadotropin and progesterone levels, culdocentesis, dilatation and curettage, transvaginal ultrasound, and laparoscopy.


Subject(s)
Pregnancy, Ectopic/diagnosis , Algorithms , Chorionic Gonadotropin/blood , Culdoscopy/methods , Dilatation and Curettage , Female , Humans , Laparoscopy , Paracentesis , Pregnancy , Progesterone/blood , Ultrasonography , Uterus/diagnostic imaging
9.
JSLS ; 16(3): 413-20, 2012.
Article in English | MEDLINE | ID: mdl-23318067

ABSTRACT

OBJECTIVE: To evaluate the efficacy and safety of culdotomy as a surgical approach to access the peritoneal cavity and discuss its implications for natural orifice transluminal endoscopic surgery (NOTES). METHODS: A retrospective chart review of women undergoing culdotomy for tubal sterilization (N 219) between January 1995 and December 2005 was performed. The Accordion Grading System was used for the severity of complications. RESULTS: No intraoperative complications were noted. Postoperative complications occurred in 7 patients (3.2%): 6 infections (grade 2) and 1 case of hemorrhage (grade 3). Conversion to laparoscopy was necessary in 10 patients (2.2%) due to anatomical constraints or pelvic adhesions; however, culdotomy with entry into the abdominal cavity was nevertheless successful in all 10 cases. The difference in the proportion with a history of pelvic surgery between the conversion and nonconversion groups was not statistically significant (P = .068). Patients with BMI ≥30 had a higher conversion rate compared to patients with BMI <30 (11.4% versus 1.5%, P = .011). Tubal sterilization via culdotomy was successfully performed in all 11 women with no prior vaginal deliveries. CONCLUSION: Culdotomy appears to be a safe surgical approach to access the peritoneal cavity and is associated with a low complication rate. These data support the feasibility and safety of utilizing the cul-de-sac as an access portal for natural orifice transluminal endoscopic surgery.


Subject(s)
Culdoscopy/methods , Natural Orifice Endoscopic Surgery/standards , Sterilization, Tubal/methods , Adult , Culdoscopy/standards , Female , Follow-Up Studies , Humans , Middle Aged , Reproducibility of Results , Retrospective Studies , Treatment Outcome , Vagina , Young Adult
10.
Semin Reprod Med ; 29(2): 95-100, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21437823

ABSTRACT

Transvaginal hydrolaparoscopy (THL) is a modification of culdoscopy that can be used to evaluate the posterior uterus, pelvic sidewalls, and adnexae. Diagnostic THL can be done in the office under local anesthesia. Combined with diagnostic hysteroscopy and chromotubation, it can replace hysterosalpingography (HSG) as the first-line diagnostic test for the infertile woman. Studies have shown high patient tolerability with less pain reported postprocedure than with HSG. THL has been shown to have a high concordance with HSG for tubal patency, but THL diagnosed more intrauterine abnormalities as well as finding adhesions and endometriosis not visible with HSG. In addition, salpingoscopy may be performed during THL to assess the tubal lumen. THL also has a high concordance rate with laparoscopy when a complete evaluation is accomplished during THL. Complications of THL are uncommon and minor. Finally, operative procedures such as ovarian drilling, coagulation of endometriosis, lysis of adhesions, treatment of ovarian cysts, and salpingostomy may be performed via THL.


Subject(s)
Culdoscopy/methods , Culdoscopy/adverse effects , Culdoscopy/instrumentation , Female , Humans , Hysterosalpingography , Hysteroscopy , Infertility, Female/diagnosis , Infertility, Female/surgery , Laparoscopy , Ovary/surgery , Polycystic Ovary Syndrome/surgery , Prognosis , Risk Factors
11.
Surg Innov ; 18(2): 114-8, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21307011

ABSTRACT

When transvaginal removal of ovarian cysts is performed successfully, the procedure compares favorably with laparoscopy in terms of invasiveness. However, the approach into peritoneal cavity has been laborious. The objective was to evaluate feasibility of an ultrasound-guided culdotomy using a newly developed umbrella needle. New culdotomy was performed on 36 patients with ovarian cysts. Each cyst was directly punctured by the needle from vagina under ultrasound guidance. The vaginal walls on both sides of the needle were incised with an electric scalpel. Through the wound, cyst was exteriorized and enucleated. Preoperative characteristics of patients, outcome, operating time, blood loss, complications, and cyst histology were analyzed. Culdotomy was performed successfully in all cases. Operating time was less than 10 minutes and blood loss was less than 10 mL. There were no culdotomy-associated complications. Culdotomy assisted by ultrasound imaging and an umbrella needle is a simple, safe, and reliable method for vaginal ovarian cystectomy.


Subject(s)
Culdoscopy/methods , Minimally Invasive Surgical Procedures/methods , Needles , Ovarian Cysts/surgery , Ultrasonography, Interventional , Adult , Cohort Studies , Equipment Design , Feasibility Studies , Female , Follow-Up Studies , Humans , Middle Aged , Minimally Invasive Surgical Procedures/instrumentation , Ovarian Cysts/diagnostic imaging , Ovariectomy/instrumentation , Ovariectomy/methods , Surgical Instruments , Treatment Outcome , Vagina/surgery , Young Adult
12.
J Minim Invasive Gynecol ; 17(6): 754-9, 2010.
Article in English | MEDLINE | ID: mdl-20656566

ABSTRACT

STUDY OBJECTIVE: To evaluate the use of a technique consisting of culdocentesis followed by saline solution-enhanced pelvic ultrasonography in cases suspect for ectopic pregnancy in which an accurate diagnosis could not be made using routine transvaginal ultrasound. DESIGN: Retrospective clinical study (Canadian Task Force classification III). SETTING: Academic medical center. PATIENTS: Twenty patients with an initial diagnosis of pregnancy of unknown location. INTERVENTIONS: In 20 patients with symptoms of early pregnancy and serum quantitative human chorionic gonadotropin concentration, ectopic pregnancy could not be confirmed or ruled out. Transvaginal ultrasound-guided culdocentesis was performed, and 300 to 400 mL of normal saline solution was injected into the posterior cul-de-sac and pelvis. Transvaginal ultrasound was repeated with particular attention to the floating fallopian tubes MEASUREMENTS AND MAIN RESULTS: Using this technique, a tubal pregnancy was visualized in 15 of 20 patients, and ectopic pregnancy was ruled out in 5 patients. In all patients, appropriate management was provided according to the final diagnosis, and consisted of either methotrexate, laparoscopic salpingostomy or salpingectomy, or expectant management in patients with abnormal intrauterine pregnancies. CONCLUSION: Ultrasound-guided culdocentesis followed by saline solution-enhanced pelvic ultrasound can be considered as a diagnostic tool in patients with suspected ectopic pregnancy in whom other methods fail to demonstrate this diagnosis.


Subject(s)
Culdoscopy/methods , Pregnancy, Ectopic/diagnosis , Ultrasonography/methods , Adolescent , Adult , Female , Humans , Pregnancy , Pregnancy, Ectopic/diagnostic imaging , Retrospective Studies , Sodium Chloride
13.
Ultrasound Obstet Gynecol ; 31(3): 342-5, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18307175

ABSTRACT

OBJECTIVES: To evaluate the feasibility and utility of performing ultrasound-guided culdotomy using a renal balloon dilator catheter for transvaginal ovarian cystectomies. METHODS: Culdotomy using transvaginal sonography and a Nephromax balloon dilator catheter was performed in 16 patients for the vaginal removal of benign ovarian cysts located in the cul-de-sac. Each ovarian cyst was punctured under transvaginal ultrasound guidance and the punctured site on the vaginal wall was enlarged with a dilator. The cyst was then enucleated through this vaginal wound. Preoperative characteristics of the patients, outcome, operating time, blood loss and complications of each culdotomy, and the histology of the cysts, were recorded and examined. RESULTS: We used this method on 14 patients with unilateral ovarian cysts and two with bilateral cysts. Culdotomy was performed successfully in 15 cases (94%). The mean +/- SD operating time for culdotomy was 22 +/- 11 min, and blood loss during the procedure was less than 10 mL in all cases. There were no complications including rectal injury or febrile morbidity. CONCLUSIONS: Culdotomy assisted by ultrasound imaging and a dilator is a safe, reliable and effective method for removal of benign ovarian cysts via a vaginal approach.


Subject(s)
Catheterization/instrumentation , Culdoscopy/methods , Ovarian Cysts/diagnostic imaging , Ovarian Cysts/surgery , Ovary/surgery , Adult , Feasibility Studies , Female , Humans , Ovary/diagnostic imaging , Treatment Outcome , Ultrasonography , Vagina
14.
Obstet Gynecol ; 109(6): 1375-80, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17540810

ABSTRACT

OBJECTIVE: To compare tubal anastomosis by robotic system compared with outpatient minilaparotomy. METHODS: In this retrospective case-control study, women were identified by current procedural terminology code for tubal anastomosis. We included all cases of tubal anastomosis for reversal of a prior tubal ligation by either outpatient minilaparotomy or robotic system technique. Cases performed by laparoscopy without aid of the robot were excluded. Comparisons were based on Fisher's exact, chi(2), and Wilcoxon rank sum tests. RESULTS: There were 26 cases of tubal anastomosis performed with the robot and 41 cases performed by outpatient minilaparotomy. The two groups were comparable in age, body mass index, and parity. Anesthesia time for the robotic technique (median with interquartile range) was 283 (267-290) minutes compared with 205 (170-230) minutes with outpatient minilaparotomy (P<.001). Surgical times for the robot and minilaparotomy were 229 (205-252) minutes and 181 (154-202) minutes respectively (P=.001). Hospitalization times, pregnancy, and ectopic pregnancy rates were not significantly different. The robotic technique was more costly. The median difference in costs of the procedures was $1,446 (95% confidence interval $1,112-1,812) (P<.001). The time to return to work was significantly shorter in the robotic system group by approximately 1 week (P=.013). CONCLUSION: Robotic surgery for tubal anastomosis was successfully accomplished without conversion to laparotomy. The robotic technique for tubal anastomosis required significantly prolonged surgical and anesthesia times over outpatient minilaparotomy (P

Subject(s)
Ambulatory Surgical Procedures/methods , Laparoscopy/methods , Laparotomy/instrumentation , Robotics/methods , Sterilization Reversal/methods , Adult , Ambulatory Surgical Procedures/economics , Ambulatory Surgical Procedures/instrumentation , Anastomosis, Surgical/instrumentation , Anastomosis, Surgical/methods , Anesthesia/methods , Case-Control Studies , Chi-Square Distribution , Culdoscopy/methods , Female , Humans , Laparoscopy/economics , Laparotomy/economics , Laparotomy/methods , Length of Stay , Middle Aged , Retrospective Studies , Robotics/economics , Statistics, Nonparametric , Sterilization Reversal/economics , Sterilization Reversal/instrumentation , Time Factors , Treatment Outcome
15.
Rev. chil. obstet. ginecol ; 72(2): 96-98, 2007.
Article in Spanish | LILACS | ID: lil-627357

ABSTRACT

ANTECEDENTES: La culdotomía como vía de acceso para realizar esterilización tubaria aparece como una alternativa simple y segura. OBJETIVO: Evaluar esta técnica en mujeres que solicitan esterilización tubaria. MÉTODO: Esterilización tubaria por culdotomía en 75 multíparas. RESULTADOS: El tiempo quirúrgico promedio fue 27,2 minutos. No hubo complicaciones durante la cirugía. En 5 casos (6,6%) la culdotomía se convirtió a laparotomía porque no se identificó una o ambas trompas. CONCLUSIÓN: Técnica segura, de bajo costo y con buena satisfacción de usuaria.


BACKGROUND: Culdotomy as access way for tubal sterilization appear like a simple and safe alternative. OBJECTIVE: To evaluate this technique in women who demand for tubal sterilization. METHOD: Tubal sterilization by culdotomy in 75 multiparous women. RESULTS: The average surgical time was 27.2 minutes. There were no complications during the surgery. In 5 cases (6.6%) the culdotomy was converted to laparotomy because one or both tubes were not identified. CONCLUSION: Culdotomy is a safe technique, of low cost and with good user satisfaction.


Subject(s)
Humans , Female , Adult , Sterilization, Tubal/methods , Culdoscopy/methods , Ambulatory Surgical Procedures , Treatment Outcome , Operative Time , Laparotomy
16.
J Vet Med A Physiol Pathol Clin Med ; 52(2): 94-8, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15737179

ABSTRACT

This study was conducted to establish a new approach for in vivo culture of in vitro produced embryos in the bovine oviduct by transvaginal endoscopy. Embryos were in vitro matured, fertilized and cultured for 1-4 days and assigned to groups consisting of 10-30 embryos. Embryos were transferred unilaterally into oviducts of 24 heifers by the means of transvaginal endoscopy. After 3-6 days of in vivo incubation embryos were re-collected. Experiment I aimed to evaluate the capability of embryos to migrate to the uterus. The uterine horns of four animals were flushed first, followed by a combined flushing of both oviducts and uterine horns resulting in collection rates of 31 and 34%, respectively. In experiment II, the transfer of embryos into the oviduct close to ovulation (day 1-2--experiment IIA) or at a more advanced cyclic stage (day 3--experiment IIB) succeeded in the collection of 46 and 34% of the transferred complexes, of which 13 and 37% showed the blastocyst stage. This is the first report of successful recovery of transferable blastocysts by transvaginal endoscopy after tubal in vivo culture in the homologous species of originally in vitro produced embryos.


Subject(s)
Cattle/embryology , Culdoscopy/veterinary , Embryo Transfer/instrumentation , Fallopian Tubes/physiology , Tissue and Organ Harvesting/veterinary , Animals , Culdoscopy/methods , Embryo Culture Techniques/methods , Embryo Culture Techniques/veterinary , Female , Fertilization in Vitro/methods , Fertilization in Vitro/veterinary , Ovulation , Vagina
17.
Fertil Steril ; 78(3): 625-7, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12215344

ABSTRACT

OBJECTIVE: To describe a technique for inserting an endoscope through the posterior vaginal fornix under direct vision using an optical cannula. DESIGN: Prospective case study. SETTING: University Department of Obstetrics & Gynecology. PATIENT(S): Patients with infertility referred for investigation in secondary care. INTERVENTION(S): Insertion of culdoscope using an optical cannula. MAIN OUTCOME MEASURE(S): Successful introduction of the culdoscope. RESULT(S): Sixteen of 20 patients had successful introduction of the optical cannula. There were no reported complications. CONCLUSION(S): Insertion of a culdoscope into the cul-de-sac can be done under visual control and this may reduce the risks of complications associated with blind insertion using a modified Veress needle.


Subject(s)
Culdoscopy/methods , Infertility, Female/diagnosis , Adult , Anovulation , Body Weight , Equipment Design , Female , Humans , Optics and Photonics
18.
J Laparoendosc Adv Surg Tech A ; 12(4): 269-71, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12269495

ABSTRACT

BACKGROUND AND PURPOSE: Oophorectomy during vaginal hysterectomy may be difficult or impossible when the ovaries lie high in the pelvis or when adhesions are present. A new technique of culdolaparoscopic oophorectomy during vaginal hysterectomy is described. PATIENTS AND METHODS: After the uterus is removed, a 12-mm cannula is introduced into the culde-sac, and a pneumoperitoneum is created. A 10-mm telescope is inserted through the vaginal port, and, under culdoscopic surveillance, two or three 3-mm abdominal ports are placed. The 10-mm telescope is removed, and a minilaparoscope is introduced through one of the abdominal cannulas. Oophorectomy is performed with a coagulator and scissors introduced via the vaginal port, and the ovaries are extracted through the vaginal cannula. Eight patients with pelvic adhesions found at the time of vaginal hysterectomy underwent culdolaparoscopic oophorectomy. RESULTS: The procedure lasted between 28 minutes and 45 minutes, including adhesiolysis, removal of an enlarged ovary, and a liver biopsy. No complications occurred in any of the patients. CONCLUSION: Culdolaparoscopic oophorectomy is a simple minimal-access surgical technique for removing the ovaries when these are not easily accessible at vaginal hysterectomy.


Subject(s)
Culdoscopy/methods , Hysterectomy, Vaginal , Laparoscopy/methods , Ovariectomy/methods , Female , Humans , Tissue Adhesions/surgery
19.
Hum Reprod ; 17(7): 1684-7, 2002 Jul.
Article in English | MEDLINE | ID: mdl-12093824

ABSTRACT

Transvaginal hydrolaparoscopy (THL) is a new culdoscopic technique for exploration of the pelvic cavity that takes advantage of micro-endoscopic technology and uses aquaflotation for inspection of the tubo-ovarian structures. In infertility patients, THL is systematically combined with mini-hysteroscopy, chromopertubation, fimbrioscopy and, when indicated, salpingoscopy. Mini-hysteroscopy in combination with the chromopertubation test allows accurate assessment of the uterine cavity and tubal patency. The transvaginal access combined with the aquaflotation during THL facilitates detailed inspection of the tubo-ovarian structures and detection of subtle pelvic disease. This combined transvaginal endoscopic approach allows complete evaluation of the reproductive tract. THL is better tolerated than hysterosalpingography, less invasive than standard laparoscopy, and can be used safely as a first line investigation of the female partner in a one-stop infertility clinic.


Subject(s)
Culdoscopy/methods , Outpatients , Cost-Benefit Analysis , Culdoscopy/economics , Culdoscopy/standards , Endoscopy , Fallopian Tubes/pathology , Female , Humans , Hysteroscopy , Ovary/pathology , Patient Acceptance of Health Care , Safety
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