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1.
Curr Drug Targets ; 21(1): 96-102, 2020.
Article in English | MEDLINE | ID: mdl-31400265

ABSTRACT

Cyclin-dependent kinase inhibitors (CDKIs) are important cell cycle regulators. The CDKI family is composed of the INK4 family and the CIP/KIP family. p19INK4d belongs to the INK4 gene family and is involved in a series of normal physiological activities and the pathogenesis of diseases. Many factors play regulatory roles in the p19INK4d gene expression at the transcriptional and posttranscriptional levels. p19INK4d not only regulates the cell cycle but also plays regulatory roles in apoptosis, DNA damage repair, cell differentiation of hematopoietic cells, and cellular senescence. In this review, the regulatory network of the p19INK4d gene expression and its biological functions are summarized, which provides a basis for further study of p19INK4d as a drug target for disease treatment.


Subject(s)
Cyclin-Dependent Kinase Inhibitor p19/physiology , Animals , Apoptosis , Cell Cycle , Cell Differentiation , Cellular Senescence , Cyclin-Dependent Kinase Inhibitor p19/biosynthesis , Cyclin-Dependent Kinase Inhibitor p19/genetics , Cyclin-Dependent Kinase Inhibitor p19/metabolism , DNA Repair , Gene Expression Regulation , Humans , Mice
2.
Cancer Res ; 69(7): 3060-8, 2009 Apr 01.
Article in English | MEDLINE | ID: mdl-19318552

ABSTRACT

NOTCH signaling is deregulated in the majority of T-cell acute lymphoblastic leukemias (T-ALL) as a result of activating mutations in NOTCH1. Gamma secretase inhibitors (GSI) block proteolytic activation of NOTCH receptors and may provide a targeted therapy for T-ALL. We have investigated the mechanisms of GSI sensitivity across a panel of T-ALL cell lines, yielding an approach for patient stratification based on pathway activity and also providing a rational combination strategy for enhanced response to GSI. Whereas the NOTCH1 mutation status does not serve as a predictor of GSI sensitivity, a gene expression signature of NOTCH pathway activity does correlate with response, and may be useful in the selection of patients more likely to respond to GSI. Furthermore, inhibition of the NOTCH pathway activity signature correlates with the induction of the cyclin-dependent kinase inhibitors CDKN2D (p19(INK4d)) and CDKN1B (p27(Kip1)), leading to derepression of RB and subsequent exit from the cell cycle. Consistent with this evidence of cell cycle exit, short-term exposure of GSI resulted in sustained molecular and phenotypic effects after withdrawal of the compound. Combination treatment with GSI and a small molecule inhibitor of CDK4 produced synergistic growth inhibition, providing evidence that GSI engagement of the CDK4/RB pathway is an important mechanism of GSI action and supports further investigation of this combination for improved efficacy in treating T-ALL.


Subject(s)
Amyloid Precursor Protein Secretases/antagonists & inhibitors , Cyclic S-Oxides/pharmacology , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/drug therapy , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/metabolism , Protease Inhibitors/pharmacology , Receptor, Notch1/antagonists & inhibitors , Retinoblastoma Protein/metabolism , Thiadiazoles/pharmacology , Cell Line, Tumor , Cyclin-Dependent Kinase 4/antagonists & inhibitors , Cyclin-Dependent Kinase Inhibitor p19/biosynthesis , Cyclin-Dependent Kinase Inhibitor p27 , G1 Phase/drug effects , G1 Phase/genetics , Gene Expression Profiling , Humans , Intracellular Signaling Peptides and Proteins/metabolism , Phosphorylation , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/pathology , Receptor, Notch1/genetics , Receptor, Notch1/metabolism , S Phase/drug effects , S Phase/genetics , Signal Transduction/drug effects , Transcription, Genetic , Transfection
3.
J Immunol ; 180(12): 8118-25, 2008 Jun 15.
Article in English | MEDLINE | ID: mdl-18523276

ABSTRACT

Inflammatory bowel disease is a chronic inflammatory response of the gastrointestinal tract mediated in part by an aberrant response to intestinal microflora. Expression of IL-23 subunits p40 and p19 within cells of the innate immune system plays a central role in the development of lower bowel inflammation in response inflammatory challenge. The NF-kappaB subunit c-Rel can regulate expression of IL-12/23 subunits suggesting that it could have a critical role in mediating the development of chronic inflammation within the lower bowel. In this study, we have analyzed the role of c-Rel within the innate immune system in the development of lower bowel inflammation, in two well-studied models of murine colitis. We have found that the absence of c-Rel significantly impaired the ability of Helicobacter hepaticus to induce colitis upon infection of RAG-2-deficient mice, and ameliorated the ability of CD4(+)CD45RB(high) T cells to induce disease upon adoptive transfer into RAG-deficient mice. The absence of c-Rel interfered with the expression of IL-12/23 subunits both in cultured primary macrophages and within the colon. Thus, c-Rel plays a critical role in regulating the innate inflammatory response to microflora within the lower bowel, likely through its ability to modulate expression of IL-12/23 family members.


Subject(s)
Colitis/immunology , Colitis/metabolism , Immunity, Innate , Proto-Oncogene Proteins c-rel/physiology , T-Lymphocyte Subsets/immunology , Animals , Chronic Disease , Colitis/microbiology , Cyclin-Dependent Kinase Inhibitor p19/biosynthesis , Cytokines/biosynthesis , Cytokines/deficiency , Cytokines/physiology , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Helicobacter Infections/genetics , Helicobacter Infections/immunology , Helicobacter Infections/microbiology , Helicobacter hepaticus/immunology , Immunity, Innate/genetics , Inflammation Mediators/metabolism , Inflammation Mediators/physiology , Interleukin-10/biosynthesis , Interleukin-10/deficiency , Interleukin-12/biosynthesis , Interleukin-12/deficiency , Interleukin-23/biosynthesis , Interleukin-23/deficiency , Mice , Mice, Knockout , Multigene Family/immunology , Proto-Oncogene Proteins c-rel/deficiency , Proto-Oncogene Proteins c-rel/genetics , Receptors, G-Protein-Coupled/biosynthesis , Up-Regulation/genetics , Up-Regulation/immunology
4.
Oncogene ; 27(12): 1677-86, 2008 Mar 13.
Article in English | MEDLINE | ID: mdl-17873901

ABSTRACT

FOXO (Forkhead box O) transcription factors are involved in cell-cycle arrest or apoptosis induction by transcripting cell-cycle inhibitor p27(KIP1) or apoptosis-related genes, respectively. Akt/protein kinase B promotes cell proliferation and suppresses apoptosis, in part, by phosphorylating FOXOs. Phosphorylated FOXOs could not exhibit transcriptional activity because of their nuclear export. Here we show that p15(INK4b) and p19(INK4d) transcription is associated with FOXO-mediated G1 cell-cycle arrest. Inhibition of Akt signaling by PI3K inhibitors, a PDK1 inhibitor, or dominant-negative Akt transfection increased expression of p15(INK4b) and p19(INK4d) but not p16(INK4a) and p18(INK4c). Ectopic expression of wild type or active FOXO but not inactive form also increased p15(INK4b) and p19(INK4d) levels. FOXOs bound to promoter regions and induced transcription of these genes. No increase in the G1-arrested cell population, mediated by PI3K inhibitor LY294002, was observed in INK4b-/- or INK4d-/- murine embryonic fibroblasts. In summary, FOXOs are involved in G1 arrest caused by Akt inactivation via p15(INK4b) and p19(INK4d) transcription.


Subject(s)
Cyclin-Dependent Kinase Inhibitor p15/genetics , Cyclin-Dependent Kinase Inhibitor p19/genetics , Forkhead Transcription Factors/physiology , Gene Expression Regulation/physiology , Animals , Cell Line , Chromones/pharmacology , Cyclin-Dependent Kinase Inhibitor p15/biosynthesis , Cyclin-Dependent Kinase Inhibitor p19/biosynthesis , Enzyme Inhibitors/pharmacology , G1 Phase/drug effects , G1 Phase/physiology , Gene Expression Regulation/drug effects , Humans , Mice , Mice, Knockout , Morpholines/pharmacology , Phosphoinositide-3 Kinase Inhibitors , Proto-Oncogene Proteins c-akt/antagonists & inhibitors , Up-Regulation/drug effects , Up-Regulation/physiology
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