ABSTRACT
We have developed a non-isotopic TR-FIA for Cyproterone acetate and Cyproterone in plasma. Synthesis of the biotinylated tracers, biotinylated Cyproterone acetate, and Cyproterone, as well as the preparation of anti-Cyproterone acetate and anti-Cyproterone antisera are reported. The specificity of anti-Cyproterone acetate antiserum resulting from the coupling of link bridge (link bridge between steroid and BSA), on the 3-position on the steroid skeleton, allowed to carry out the Cyproterone acetate assay directly on extracted plasma (without chromatography). On the other hand Cyproterone assays require a purification step, including extraction plus chromatography, because the plasma Cyproterone acetate concentrations in Cyproterone acetate-treated women are 200 times higher than for Cyproterone. Theses plasma TR-FIA of Cyproterone acetate and Cyproterone presented the advantage of needing only small doses of radioactivity for recovery controls, and better practicability related to the only existing RIA described to date.
Subject(s)
Blood Chemical Analysis/methods , Cyproterone Acetate/blood , Cyproterone/blood , Fluoroimmunoassay/methods , Acne Vulgaris/blood , Acne Vulgaris/drug therapy , Animals , Antibody Specificity , Blood Chemical Analysis/standards , Blood Chemical Analysis/statistics & numerical data , Cyproterone/immunology , Cyproterone Acetate/immunology , Cyproterone Acetate/therapeutic use , Female , Fluoroimmunoassay/standards , Fluoroimmunoassay/statistics & numerical data , Hirsutism/blood , Hirsutism/drug therapy , Humans , Rabbits , Reference Standards , Sensitivity and SpecificitySubject(s)
Antibodies/analysis , Cyproterone/analogs & derivatives , Iliac Vein , Thrombosis/chemically induced , Adult , Androgen Antagonists/immunology , Cyproterone/adverse effects , Cyproterone/immunology , Cyproterone Acetate , Drug Combinations/adverse effects , Ethinyl Estradiol/adverse effects , Female , HumansABSTRACT
A sensitive and specific radioimmunoassay for cyproterone acetate in serum has been developed. Antiserum was raised in rabbits by immunization with a conjugate of cyproterone acetate-11 alpha-hemisuccinate and bovine serum albumin. Radioactive tracers with high specific activities were synthesized by coupling the hemisuccinate with [3H]-tyrosine methyl ester of [125I]-iodohistamine. Competition studies with substances structurally related to cyproterone acetate have revealed that the structures of ring A and B are mainly responsible for binding to the antibody.
