ABSTRACT
OBJECTIVE: To study the secondary metabolites of mangrove endophytic fungus BL321. METHODS: The compounds were isolated by chromatographic technique. The structures were identified by comprehensive physico-chemical properties and spectral methods. RESULTS: Five compounds were isolated and identified as 3,4a-dimethyl-2-oxo-2,4,4a,5,6,7-hexahydronaphtho[2,3-b]furan-5-carboxylic acid(1), cytochalasin C(2), cytochalasin D(3), 19,20-epoxycytochalasin C(4), ergosterol(5). CONCLUSION: Compound 1 is isolated from nature for the first time. Further more, several kinds of strong bioactive compounds were islolate from this fungus indicate that it may develop to be medical source microorganism.
Subject(s)
Cytochalasins/isolation & purification , Ergosterol/isolation & purification , Fungi/chemistry , Furans/isolation & purification , Naphthalenes/isolation & purification , Rhizophoraceae/microbiology , China , Cytochalasin D/chemistry , Cytochalasin D/isolation & purification , Cytochalasins/chemistry , Ergosterol/chemistry , Fungi/metabolism , Furans/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Naphthalenes/chemistryABSTRACT
Chemical investigation of the wood-decayed fungus Xylaria sp. BCC 9653 has led to the isolation of a new methyl aminobenzoate (1) together with eleven known compounds. The structures were established by analysis of spectroscopic data. Cytochalasin D (2), one of the known metabolites, exhibited potent cytotoxicity against African green monkey kidney fibroblast (Vero) cells with an IC(50) value of 0.19 microM.
Subject(s)
Antiviral Agents/pharmacology , Cytochalasin D/pharmacology , Fibroblasts/drug effects , Kidney/drug effects , Wood , Xylariales/chemistry , para-Aminobenzoates , 4-Aminobenzoic Acid/chemistry , 4-Aminobenzoic Acid/isolation & purification , 4-Aminobenzoic Acid/pharmacology , Animals , Antiviral Agents/chemistry , Antiviral Agents/isolation & purification , Cells, Cultured , Chlorocebus aethiops , Cytochalasin D/chemistry , Cytochalasin D/isolation & purification , Fibroblasts/cytology , Inhibitory Concentration 50 , Kidney/cytology , Kidney/metabolism , Molecular Structure , Spectrum Analysis , Vero Cells/drug effectsABSTRACT
Cytochalasin D which shows marked cytotoxic effects on multi-tumor cells was newly isolated at high content(5.28 mg/g, dry weight) from Fungus Hypocrella bambusae(B.et Br.) Sacc. Its structure was elucidated by spectroscopic methods. Two-dimensional NMR techniques were applied to make complete assignment for the 1H- and 13C-NMR chemical shifts of this compound.
