ABSTRACT
Commensal lactobacilli that produce hydrogen peroxide (H(2)O(2)) inhibit Neisseria gonorrhoeae in vitro, and clinical data suggest that they are associated with a reduced risk of gonorrhea. We precolonized mice with Lactobacillus crispatus and then challenged them with N. gonorrhoeae, to measure the effects of H(2)O(2)-producing lactobacilli on gonococcal infection. We found no difference in the duration of infection or the number of gonococci recovered from untreated mice and mice colonized with L. crispatus. A gonococcal catalase mutant and a catalase, cytochrome C peroxidase mutant exhibited greater susceptibility to L. crispatus in vitro than did wild-type bacteria; however, recovery of these mutants from mice was not affected by L. crispatus. We also found no evidence that utilization of lactobacillus-produced lactate by N. gonorrhoeae balances the detrimental effects of H(2)O(2) during infection. We conclude that the association between lactobacilli and gonococci is complex and may be subject to factors that have not been reproduced in vitro.
Subject(s)
Gonorrhea/microbiology , Hydrogen Peroxide/metabolism , Hydrogen Peroxide/pharmacology , Lactobacillus/metabolism , Neisseria gonorrhoeae/genetics , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Catalase/drug effects , Catalase/genetics , Catalase/metabolism , Cytochrome-c Peroxidase/deficiency , Cytochrome-c Peroxidase/genetics , Cytochrome-c Peroxidase/metabolism , Female , Genetic Predisposition to Disease , Gonorrhea/drug therapy , Gonorrhea/enzymology , Humans , Hydrogen-Ion Concentration , Kinetics , Lactobacillus/growth & development , Neisseria gonorrhoeae/drug effects , Neisseria gonorrhoeae/growth & development , Vagina/physiopathologyABSTRACT
It has been found that yeast mutants deficient in cytosolic superoxide dismutase CuZnSOD are hypersensitive to ferrous iron. In contrast mutants that are deficient in catalases and cytochrome c peroxidase do not differ from the standard strain in this respect. These findings suggest that iron toxicity may depend on the redox status of the cell. They also shed light on the role of superoxide dismutases in preventing the toxic effects of oxygen.