DNA injury induced by 5-aminouracil and caffeine in G2 checkpoints path of higher plant cells

This work evaluated the qualitative and quantitative cellular changes induced by treatment with 5-aminouracil (5-AU) and a combination of 5-AU and caffeine in plant cells in relation to DNA damage, repaired damage, and residual damage. As biological material, Allium cepa L. root tips were used, grown in filtered water, in darkness, with aeration at constant temperature of 25ºC ± 0.5. Cell populations were synchronized using 5 mM caffeine in order to study the effects of 5-AU and caffeine/5-AU combined treatment on the DNA content and their incidence in the entrance to mitosis. The results showed a delay in the G2 period due t

Evaluación del rol de los DNA-tests en el diagnóstico de la patología del cuello uterino / Evaluation of the role of DNA tsting in the diagnosis of uterine cervical cancer

This study aimed to analyze the role of DNA tsting in cervical cancer. In 1995, the International Agency for Research on Cancer defined the association between infections with human papilloma virus (HPV) and cervical cancer (CC), sustained in numerous epidemiologic and experimental evidences. However, even if the infection develops as a necessary condition to generate a CC in almost the 100 por ciento of the cases, it would not be sufficient enough without the support of other associated factors to break out the tumoral growth. Thus, human papilloma viruses are receiving increasing attention for their role in the pathogenesis of ulterine cervix cancer, and therefore efforts should be dedicated to avoid this infection as a mean to prevent CC, development of an effective vaccine. We examined a group of 111 females in order to evaluate the results obtained with human papilloma virus DNA testing aimed to generate indications to improve the managements condition of cost-effectiveness

Estudios bioquímicos y caracterización molecular de pacientes portadores de ß talasemia en Córdoba, Argentina / Biochemical studies and molecular characterization of ß-thalassemia carriers in Córdoba, Argentina

Las ß talasemias constituyen un grupo heterogéneo de anemias microcíticas, causadas por una producción reducida (ß+) o ausencia (ߺ) de síntesis de cadenas de globina ß. La diferente expresividad clínica de estas patologías resulta de la combinación de ambas posibilidades o de cada una de ellas con el gen normal. Dado que este país ha recibido distintas corrientes migratorias, y que

Aplicaciones diagnósticas de secuencias repetitivas de ADN / Diagnostics applications of repetitive DNA sequences

Se discute el potencial, las ventajas y las diferentes áreas de aplicaciones diagnósticas para varias categorías de secuencias repetitivas de ADN. Dado que todos los eucariotas se caracterizan por la redundancia genómica, estas técnicas sensibles, rápidas y comparativamente simples han revolucionado el campo del diagnóstico clínico experimental (AU)

Sarcoma de células claras (melanoma maligno de partes blandas): estudio clínico-patológico; inmunohistoquímico y de la ploidía nuclear, a propósito de un caso / Clear cell sarcoma (soft tissue malignant melanoma): a clinic, pathological, immunohistochemical and nuclear ploidy study, apropos of case

Se presenta un caso de melanoma maligno de partes blandas (talón) estudiando los criterios histológicos e inmunohistoquímicos para su certeza diagnóstica, así como la determinación de la ploidia nuclear. Se establecen las pautas de estadificación y tratamiento. Se señala su mal pronóstico y su alto porcentaje de recurrencias locales y metástasis a distancia