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1.
J Physiol ; 601(9): 1583-1595, 2023 05.
Article in English | MEDLINE | ID: mdl-36479972

ABSTRACT

Ion channels of the degenerin (DEG)/epithelial Na+ channel (ENaC) family serve diverse functions ranging from mechanosensation over Na+ reabsorption to H+ sensing and neurotransmission. However, several diverse DEG/ENaCs interact with neuropeptides; some are directly activated, whereas others are modulated by neuropeptides. Two questions arise: does this interaction have a common structural basis and does it have an ancient origin? Current evidence suggests that RFamide neuropeptides activate the FMRFamide-activated Na+ channels (FaNaCs) of invertebrates via binding to a pocket at the external face of their large extracellular domain. It is likely that RFamides might activate DEG/ENaCs from the freshwater polyp Hydra (the HyNaCs) via binding to a similar pocket, although there is not yet any experimental evidence. In contrast, RFamide neuropeptides modulate acid-sensing ion channels (ASICs) from vertebrates via binding to a central cavity enclosed by ß-sheets of the extracellular domain. Dynorphin opioid peptides, for their part, bind to the acidic pocket of ASICs, which might be evolutionarily related to the peptide binding pocket of FaNaCs, but instead of opening the channels they work as antagonists to stabilize its closed state. Moreover, peptides interacting with DEG/ENaCs from animals of different phyla, although having similar sequences, are evolutionarily unrelated to each other. Collectively, it appears that despite a seemingly similar interaction with similar peptides, the interaction of DEG/ENaCs with neuropeptides has diverse structural bases and many origins.


Subject(s)
Cnidaria , Neuropeptides , Animals , Degenerin Sodium Channels/metabolism , Cnidaria/metabolism , Neuropeptides/metabolism , Peptides , Acid Sensing Ion Channels/metabolism , Ions/metabolism , Mammals/metabolism , Epithelial Sodium Channels/metabolism
2.
Am J Hypertens ; 34(11): 1227-1235, 2021 11 20.
Article in English | MEDLINE | ID: mdl-34161569

ABSTRACT

BACKGROUND: Pressure-induced constriction (PIC) is inherent to small arteries and arterioles, in which intraluminal pressure-induced vascular smooth muscle cell stretch elicits vasoconstriction. Degenerin (Deg) proteins, such as beta-epithelial Na+ channel (ßENaC), have been studied in the PIC response because they are evolutionarily linked to known mechanosensors. While loss of Deg function phenotypes are plentiful, a gain-of-function phenotype has not been studied. The aim of this study was to determine if expression of exogenous ßENaC in the isolated middle cerebral artery (MCA) enhances the PIC response. METHODS: Isolated MCA segments from female mice (24 weeks, n = 5) were transfected with enhanced green fluorescent protein-ßENaC (EGFP-ßENaC) or with EGFP alone, incubated overnight at 37 °C, then studied in a pressure myograph. RESULTS: Mechanical/morphological properties and vasoconstrictor responses to KCl and phenylephrine were identical in EGFP-ßENaC and EGFP MCAs. In contrast, PIC responses were greater in EGFP-ßENaC segments with ~2-fold greater peak myogenic tone. CONCLUSIONS: These data confirm previous findings that ßENaC is critical in the PIC response. These data provide proof-of-concept that upregulating ßENaC can enhance PIC responses and lay the foundation to test the hypothesis that inflammation-mediated downregulation of ßENaC contributes to cerebrovascular dysfunction.


Subject(s)
Epithelial Sodium Channels , Middle Cerebral Artery , Animals , Constriction , Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/genetics , Female , Mice , Muscle, Smooth, Vascular/metabolism , Phenylephrine/pharmacology , Sodium/metabolism , Vasoconstriction , Vasoconstrictor Agents/pharmacology
3.
Int J Mol Sci ; 22(9)2021 May 01.
Article in English | MEDLINE | ID: mdl-34062742

ABSTRACT

Acid-sensing ion channels (ASICs) are mainly proton-gated cation channels that are activated by pH drops and nonproton ligands. They are part of the degenerin/epithelial sodium channel superfamily due to their sodium permeability. Predominantly expressed in the central nervous system, ASICs are involved in synaptic plasticity, learning/memory, and fear conditioning. These channels have also been implicated in multiple disease conditions, including ischemic brain injury, multiple sclerosis, Alzheimer's disease, and drug addiction. Recent research has illustrated the involvement of ASICs in mechanosensation. Mechanosensation is a form of signal transduction in which mechanical forces are converted into neuronal signals. Specific mechanosensitive functions have been elucidated in functional ASIC1a, ASIC1b, ASIC2a, and ASIC3. The implications of mechanosensation in ASICs indicate their subsequent involvement in functions such as maintaining blood pressure, modulating the gastrointestinal function, and bladder micturition, and contributing to nociception. The underlying mechanism of ASIC mechanosensation is the tether-gate model, which uses a gating-spring mechanism to activate ASIC responses. Further understanding of the mechanism of ASICs will help in treatments for ASIC-related pathologies. Along with the well-known chemosensitive functions of ASICs, emerging evidence has revealed that mechanosensitive functions of ASICs are important for maintaining homeostasis and contribute to various disease conditions.


Subject(s)
Acid Sensing Ion Channels/genetics , Degenerin Sodium Channels/genetics , Mechanotransduction, Cellular/genetics , Neurons/metabolism , Acid Sensing Ion Channels/metabolism , Animals , Degenerin Sodium Channels/metabolism , Humans , Multiple Sclerosis/genetics , Multiple Sclerosis/pathology , Neuronal Plasticity , Sodium/metabolism
4.
PLoS Genet ; 17(2): e1009066, 2021 02.
Article in English | MEDLINE | ID: mdl-33571185

ABSTRACT

Intracellular Ca2+ level is under strict regulation through calcium channels and storage pools including the endoplasmic reticulum (ER). Mutations in certain ion channel subunits, which cause mis-regulated Ca2+ influx, induce the excitotoxic necrosis of neurons. In the nematode Caenorhabditis elegans, dominant mutations in the DEG/ENaC sodium channel subunit MEC-4 induce six mechanosensory (touch) neurons to undergo excitotoxic necrosis. These necrotic neurons are subsequently engulfed and digested by neighboring hypodermal cells. We previously reported that necrotic touch neurons actively expose phosphatidylserine (PS), an "eat-me" signal, to attract engulfing cells. However, the upstream signal that triggers PS externalization remained elusive. Here we report that a robust and transient increase of cytoplasmic Ca2+ level occurs prior to the exposure of PS on necrotic touch neurons. Inhibiting the release of Ca2+ from the ER, either pharmacologically or genetically, specifically impairs PS exposure on necrotic but not apoptotic cells. On the contrary, inhibiting the reuptake of cytoplasmic Ca2+ into the ER induces ectopic necrosis and PS exposure. Remarkably, PS exposure occurs independently of other necrosis events. Furthermore, unlike in mutants of DEG/ENaC channels, in dominant mutants of deg-3 and trp-4, which encode Ca2+ channels, PS exposure on necrotic neurons does not rely on the ER Ca2+ pool. Our findings indicate that high levels of cytoplasmic Ca2+ are necessary and sufficient for PS exposure. They further reveal two Ca2+-dependent, necrosis-specific pathways that promote PS exposure, a "two-step" pathway initiated by a modest influx of Ca2+ and further boosted by the release of Ca2+ from the ER, and another, ER-independent, pathway. Moreover, we found that ANOH-1, the worm homolog of mammalian phospholipid scramblase TMEM16F, is necessary for efficient PS exposure in thapsgargin-treated worms and trp-4 mutants, like in mec-4 mutants. We propose that both the ER-mediated and ER-independent Ca2+ pathways promote PS externalization through activating ANOH-1.


Subject(s)
Caenorhabditis elegans/metabolism , Calcium/metabolism , Endoplasmic Reticulum/metabolism , Neurons/metabolism , Phosphatidylserines/metabolism , Animals , Animals, Genetically Modified , Apoptosis/genetics , Caenorhabditis elegans/drug effects , Caenorhabditis elegans/genetics , Caenorhabditis elegans Proteins/genetics , Caenorhabditis elegans Proteins/metabolism , Cytoplasm/metabolism , Dantrolene/pharmacology , Degenerin Sodium Channels/genetics , Degenerin Sodium Channels/metabolism , Endoplasmic Reticulum/drug effects , Endoplasmic Reticulum/genetics , Enzyme Inhibitors/pharmacology , Membrane Proteins/genetics , Membrane Proteins/metabolism , Muscle Relaxants, Central/pharmacology , Necrosis/genetics , Necrosis/metabolism , Neurons/drug effects , Neurons/pathology , Phospholipid Transfer Proteins/genetics , Phospholipid Transfer Proteins/metabolism , Sodium Channels/genetics , Sodium Channels/metabolism , TRPC Cation Channels/genetics , TRPC Cation Channels/metabolism , Thapsigargin/pharmacology
5.
J Biol Chem ; 294(44): 16320-16336, 2019 11 01.
Article in English | MEDLINE | ID: mdl-31527080

ABSTRACT

Acid-sensitive ion channels belonging to the degenerin/epithelial sodium channel (DEG/ENaC) family activate in response to extracellular protons and are considered unique to deuterostomes. However, sensitivity to pH/protons is more widespread, where, for example, human ENaC Na+ leak channels are potentiated and mouse BASIC and Caenorhabditis elegans ACD-1 Na+ leak channels are blocked by extracellular protons. For many DEG/ENaC channels, extracellular Ca2+ ions modulate gating, and in some cases, the binding of protons and Ca2+ is interdependent. Here, we functionally characterize a DEG/ENaC channel from the early-diverging animal Trichoplax adhaerens, TadNaC6, that conducts Na+-selective leak currents in vitro sensitive to blockade by both extracellular protons and Ca2+ We determine that proton block is enhanced in low external Ca2+ concentration, whereas calcium block is enhanced in low external proton concentration, indicative of competitive binding of these two ligands to extracellular sites of the channel protein. TadNaC6 lacks most determinant residues for proton and Ca2+ sensitivity in other DEG/ENaC channels, and a mutation of one conserved residue (S353A) associated with Ca2+ block in rodent BASIC channels instead affected proton sensitivity, all indicative of independent evolution of H+ and Ca2+ sensitivity. Strikingly, TadNaC6 was potently activated by the general DEG/ENaC channel blocker amiloride, a rare feature only reported for the acid-activated channel ASIC3. The sequence and structural divergence of TadNaC6, coupled with its noncanonical functional features, provide unique opportunities for probing the proton, Ca2+, and amiloride regulation of DEG/ENaC channels and insight into the possible core-gating features of ancestral ion channels.


Subject(s)
Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Placozoa/metabolism , Animals , CHO Cells , Calcium/metabolism , Cricetulus , Hydrogen-Ion Concentration , Ion Channel Gating/physiology , Ion Transport , Ions/metabolism , Protons , Receptors, Calcium-Sensing/metabolism , Sodium/metabolism , Sodium Channels/metabolism
6.
Channels (Austin) ; 12(1): 262-275, 2018.
Article in English | MEDLINE | ID: mdl-30001175

ABSTRACT

Degenerin/Epithelial Sodium Channels (DEG/ENaCs) are a large family of animal-specific non-voltage gated ion channels, with enriched expression in neuronal and epithelial tissues. While neuronal DEG/ENaCs were originally characterized as sensory receptor channels, recent studies indicate that several DEG/ENaC family members are also expressed throughout the central nervous system. Human genome-wide association studies have linked DEG/ENaC-coding genes with several neurologic and psychiatric disorders, including epilepsy and panic disorder. In addition, studies in rodent models further indicate that DEG/ENaC activity in the brain contributes to many behaviors, including those related to anxiety and long-term memory. Although the exact neurophysiological functions of DEG/ENaCs remain mostly unknown, several key studies now suggest that multiple family members might exert their neuronal function via the direct modulation of synaptic processes. Here, we review and discuss recent findings on the synaptic functions of DEG/ENaCs in both vertebrate and invertebrate species, and propose models for their possible roles in synaptic physiology.


Subject(s)
Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Animals , Humans , Synaptic Transmission
7.
Pflugers Arch ; 470(7): 1087-1102, 2018 07.
Article in English | MEDLINE | ID: mdl-29589117

ABSTRACT

The bile acid-sensitive ion channel (BASIC) is a member of the ENaC/degenerin family of ion channels. It is activated by bile acids and inhibited by extracellular Ca2+. The aim of this study was to explore the molecular mechanisms mediating these effects. The modulation of BASIC function by extracellular Ca2+ and tauro-deoxycholic acid (t-DCA) was studied in Xenopus laevis oocytes heterologously expressing human BASIC using the two-electrode voltage-clamp and outside-out patch-clamp techniques. Substitution of aspartate D444 to alanine or cysteine in the degenerin region of BASIC, a region known to be critically involved in channel gating, resulted in a substantial reduction of BASIC Ca2+ sensitivity. Moreover, mutating D444 or the neighboring alanine (A443) to cysteine significantly reduced the t-DCA-mediated BASIC stimulation. A combined molecular docking/simulation approach demonstrated that t-DCA may temporarily form hydrogen bonds with several amino acid residues including D444 in the outer vestibule of the BASIC pore or in the inter-subunit space. By these interactions, t-DCA may stabilize the open state of the channel. Indeed, single-channel recordings provided evidence that t-DCA activates BASIC by stabilizing the open state of the channel, whereas extracellular Ca2+ inhibits BASIC by stabilizing its closed state. In conclusion, our results highlight the potential role of the degenerin region as a critical regulatory site involved in the functional interaction of Ca2+ and t-DCA with BASIC.


Subject(s)
Bile Acids and Salts/metabolism , Calcium/metabolism , Degenerin Sodium Channels/metabolism , Amino Acid Sequence , Animals , Bile/metabolism , Humans , Ion Channel Gating/physiology , Molecular Docking Simulation/methods , Oocytes/metabolism , Xenopus laevis/metabolism
8.
Biophys J ; 114(6): 1321-1335, 2018 03 27.
Article in English | MEDLINE | ID: mdl-29590590

ABSTRACT

The bile acid-sensitive ion channel is activated by amphiphilic substances such as bile acids or artificial detergents via membrane alterations; however, the mechanism of membrane sensitivity of the bile acid-sensitive ion channel is not known. It has also not been systematically investigated whether other members of the degenerin/epithelial Na+ channel (DEG/ENaC) gene family are affected by amphiphilic compounds. Here, we show that DEG/ENaCs ASIC1a, ASIC3, ENaC, and the purinergic receptor P2X2 are modulated by a large number of different, structurally unrelated amphiphilic substances, namely the detergents N-lauroylsarcosine, Triton X-100, and ß-octylglucoside; the fenamate flufenamic acid; the antipsychotic drug chlorpromazine; the natural phenol resveratrol; the chili pepper compound capsaicin; the loop diuretic furosemide; and the antiarrythmic agent verapamil. We determined the modification of membrane properties using large-angle x-ray diffraction experiments on model lipid bilayers, revealing that the amphiphilic compounds are positioned in a characteristic fashion either in the lipid tail group region or in the lipid head group region, demonstrating that they perturbed the membrane structure. Collectively, our results show that DEG/ENaCs and structurally related P2X receptors are modulated by diverse amphiphilic molecules. Furthermore, they suggest alterations of membrane properties by amphiphilic compounds as a mechanism contributing to modulation.


Subject(s)
Cell Membrane/drug effects , Cell Membrane/metabolism , Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Hydrophobic and Hydrophilic Interactions , Animals , Rats
9.
Pflugers Arch ; 467(1): 39-48, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25053538

ABSTRACT

Mechanosensory neurons, whose activity is controlled by mechanical force, underlie the senses of touch, hearing, and proprioception, yet despite their importance, the molecular basis of mechanotransduction is poorly understood. Genetic studies in Caenorhabditis elegans have provided a useful approach for identifying potential components of mechanotransduction complexes that might be conserved in more complex organisms. This review describes the mechanosensory systems of C. elegans, including the sensory neurons and circuitry involved in body touch, nose touch, and proprioception. In addition, the roles of genes encoding known and potential mechanosensory receptors, including members of the broadly conserved transient receptor potential (TRP) and degerin/epithelial Na(+) channel (DEG/ENaC) channel families, are discussed.


Subject(s)
Caenorhabditis elegans Proteins/metabolism , Caenorhabditis elegans/physiology , Mechanoreceptors/physiology , Mechanotransduction, Cellular/physiology , Proprioception/physiology , Touch/physiology , Animals , Degenerin Sodium Channels/metabolism , Models, Biological , Transient Receptor Potential Channels/metabolism
10.
Curr Biol ; 24(24): 2920-5, 2014 Dec 15.
Article in English | MEDLINE | ID: mdl-25454784

ABSTRACT

The Drosophila gene pickpocket (ppk) encodes an ion channel subunit of the degenerin/epithelial sodium channel (DEG/ENaC) family. PPK is specifically expressed in nociceptive, class IV multidendritic (md) neurons and is functionally required for mechanical nociception responses. In this study, in a genome-wide genetic screen for other ion channel subunits required for mechanical nociception, we identify a gene that we name balboa (also known as CG8546, ppk26). Interestingly, the balboa locus encodes a DEG/ENaC ion channel subunit highly similar in amino acid sequence to PPK. Moreover, laser-capture isolation of RNA from larval neurons and microarray analyses reveal that balboa is also highly enriched in nociceptive neurons. The requirement for Balboa and PPK in mechanical nociception behaviors and their specific expression in larval nociceptors led us to hypothesize that these DEG/ENaC subunits form an ion channel complex in vivo. In nociceptive neurons, Balboa::GFP proteins distribute uniformly throughout dendrites but remarkably localize to discrete foci when ectopically expressed in other neuron subtypes (where PPK is not expressed). Indeed, ectopically coexpressing ppk transforms this punctate Balboa::GFP expression pattern to the uniform distribution observed in its native cell type. Furthermore, ppk-RNAi in class IV neurons alters the broad Balboa::GFP pattern to a punctate distribution. Interestingly, this interaction is mutually codependent as balboa-RNAi eliminates Venus::PPK from the sensory dendrites of nociceptors. Finally, using a GFP-reconstitution approach in transgenic larvae, we directly detect in vivo physical interactions among PPK and Balboa subunits. Combined, our results indicate a critical mechanical nociception function for heteromeric PPK and Balboa channels in vivo.


Subject(s)
Drosophila Proteins/genetics , Drosophila melanogaster/physiology , Epithelial Sodium Channels/genetics , Nociception , Sodium Channels/genetics , Animals , Animals, Genetically Modified/genetics , Animals, Genetically Modified/growth & development , Animals, Genetically Modified/physiology , Degenerin Sodium Channels/genetics , Degenerin Sodium Channels/metabolism , Dendrites/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/genetics , Drosophila melanogaster/growth & development , Epithelial Sodium Channels/metabolism , Larva/physiology , Oligonucleotide Array Sequence Analysis , Sequence Analysis, Protein , Sodium Channels/metabolism
11.
Cell Rep ; 9(4): 1446-58, 2014 Nov 20.
Article in English | MEDLINE | ID: mdl-25456135

ABSTRACT

A major gap in our understanding of sensation is how a single sensory neuron can differentially respond to a multitude of different stimuli (polymodality), such as propio- or nocisensation. The prevailing hypothesis is that different stimuli are transduced through ion channels with diverse properties and subunit composition. In a screen for ion channel genes expressed in polymodal nociceptive neurons, we identified Ppk26, a member of the trimeric degenerin/epithelial sodium channel (DEG/ENaC) family, as being necessary for proper locomotion behavior in Drosophila larvae in a mutually dependent fashion with coexpressed Ppk1, another member of the same family. Mutants lacking Ppk1 and Ppk26 were defective in mechanical, but not thermal, nociception behavior. Mutants of Piezo, a channel involved in mechanical nociception in the same neurons, did not show a defect in locomotion, suggesting distinct molecular machinery for mediating locomotor feedback and mechanical nociception.


Subject(s)
Behavior, Animal , Degenerin Sodium Channels/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/physiology , Epithelial Sodium Channels/metabolism , Locomotion , Sodium Channels/metabolism , Animals , Cell Membrane/metabolism , Dendrites/metabolism , Mutation/genetics , Nociception , Protein Binding , Protein Subunits/metabolism , Temperature
12.
FASEB J ; 27(12): 5034-45, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24018065

ABSTRACT

Epithelial Na(+) channel (ENaC)/degenerin family members are involved in mechanosensation, blood pressure control, pain sensation, and the expression of fear. Several of these channel types display a form of desensitization that allows the channel to limit Na(+) influx during prolonged stimulation. We used site-directed mutagenesis and chemical modification, functional analysis, and molecular dynamics simulations to investigate the role of the lower palm domain of the acid-sensing ion channel 1, a member of the ENaC/degenerin family. The lower palm domains of this trimeric channel are arranged around a central vestibule, at ∼20 Šabove the plasma membrane and are covalently linked to the transmembrane channel parts. We show that the lower palm domains approach one another during desensitization. Residues in the palm co-determine the pH dependence of desensitization, its kinetics, and the stability of the desensitized state. Mutations of palm residues impair desensitization by preventing the closing movement of the palm. Overexpression of desensitization-impaired channel mutants in central neurons allowed--in contrast to overexpression of wild type--a sustained signaling response to rapid pH fluctuations. We identify and describe here the function of an important regulatory domain that most likely has a conserved role in ENaC/degenerin channels.


Subject(s)
Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Ion Channel Gating , Amino Acid Sequence , Animals , Degenerin Sodium Channels/chemistry , Degenerin Sodium Channels/genetics , Epithelial Sodium Channels/chemistry , Epithelial Sodium Channels/genetics , Hydrogen-Ion Concentration , Molecular Dynamics Simulation , Molecular Sequence Data , Point Mutation , Protein Structure, Tertiary , Rats , Rats, Sprague-Dawley , Xenopus
13.
Curr Mol Pharmacol ; 6(1): 44-9, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23547934

ABSTRACT

The epithelial sodium channel/degenerin (ENaC/deg) family of ion channels is formed by a large number of genes with variable tissue expression patterns and physiological roles. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. ENaC is formed by three homologous subunits, α, ß and γ, and a fourth subunit (δ) has been found in human and monkeys that can substitute α to form functional channels. The best-characterized role of ENaC is to serve as a rate-limiting step in transepithelial sodium reabsorption in the distal part of the kidney tubule and other tight epithelia. However, ENaC subunits are also found in the peripheral and central nervous system, where their functional roles are only beginning to be understood. In this review, we mainly focus on the putative pathophysiological roles of ENaC channels in the central nervous system and their potential value as drug targets in neurodegenerative disorders and the central control of blood pressure.


Subject(s)
Brain/metabolism , Epithelial Sodium Channels/metabolism , Acid Sensing Ion Channels/metabolism , Animals , Central Nervous System/metabolism , Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/chemistry , Epithelial Sodium Channels/genetics , Humans , Protein Subunits/chemistry , Protein Subunits/genetics , Protein Subunits/metabolism
14.
J Biol Chem ; 288(13): 9418-27, 2013 Mar 29.
Article in English | MEDLINE | ID: mdl-23404498

ABSTRACT

The voltage-gated K(+) (Kv) channel blocker 4-aminopyridine (4-AP) is used to target symptoms of the neuroinflammatory disease multiple sclerosis (MS). By blocking Kv channels, 4-AP facilitates action potential conduction and neurotransmitter release in presynaptic neurons, lessening the effects of demyelination. Because they conduct inward Na(+) and Ca(2+) currents that contribute to axonal degeneration in response to inflammatory conditions, acid-sensing ion channels (ASICs) contribute to the pathology of MS. Consequently, ASICs are emerging as disease-modifying targets in MS. Surprisingly, as first demonstrated here, 4-AP inhibits neuronal degenerin/epithelial Na(+) (Deg/ENaC) channels, including ASIC and BLINaC. This effect is specific for 4-AP compared with its heterocyclic base, pyridine, and the related derivative, 4-methylpyridine; and akin to the actions of 4-AP on the structurally unrelated Kv channels, dose- and voltage-dependent. 4-AP has differential actions on distinct ASICs, strongly inhibiting ASIC1a channels expressed in central neurons but being without effect on ASIC3, which is enriched in peripheral sensory neurons. The voltage dependence of the 4-AP block and the single binding site for this inhibitor are consistent with 4-AP binding in the pore of Deg/ENaC channels as it does Kv channels, suggesting a similar mechanism of inhibition in these two classes of channels. These findings argue that effects on both Kv and Deg/ENaC channels should be considered when evaluating the actions of 4-AP. Importantly, the current results are consistent with 4-AP influencing the symptoms of MS as well as the course of the disease because of inhibitory actions on Kv and ASIC channels, respectively.


Subject(s)
4-Aminopyridine/pharmacology , Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Multiple Sclerosis/metabolism , Neurons/metabolism , Animals , Animals, Newborn , CHO Cells , Cricetinae , Drosophila melanogaster , Hippocampus/metabolism , Inhibitory Concentration 50 , Mice , Mice, Inbred C57BL , Neurodegenerative Diseases/metabolism , Patch-Clamp Techniques , Potassium Channel Blockers/pharmacology , Potassium Channels/metabolism , Rats , Rats, Sprague-Dawley
15.
J Neurosci ; 33(3): 936-49, 2013 Jan 16.
Article in English | MEDLINE | ID: mdl-23325233

ABSTRACT

Neuronal DEG/ENaC (degenerin and epithelial Na(+) channel) Na(+) channels have been implicated in touch sensation. For example, MEC-4 is expressed in touch neurons in Caenorhabditis elegans and mediates gentle-touch response. Similarly, homologous mammalian ASIC2 and ASIC3 are expressed in sensory neurons and produce touch phenotypes when knocked out in mice. Here, we show that novel DEG/ENaC subunits DELM-1 and DELM-2 (degenerin-like channel mechanosensory linked-1 and degenerin-like channel mechanosensory linked-2) are expressed in glia associated with touch neurons in C. elegans and that their knock-out causes defects in mechanosensory behaviors related to nose touch and foraging, which are mediated by OLQ and IL1 sensory neurons. Cell-specific rescue supports that DELM-1 and DELM-2 are required cell-autonomously in glia to orchestrate mechanosensory behaviors. Electron microscopy reveals that in delm-1 knock-outs, OLQ and IL1 sensory neurons and associated glia are structurally normal. Furthermore, we show that knock-out of DELM-1 and DELM-2 does not disrupt the expression or cellular localization of TRPA-1, a TRP channel needed in OLQ and IL1 neurons for touch behaviors. Rather, rescue of the delm-1 nose-touch-insensitive phenotype by expression of a K(+) channel in socket glia and of a cationic channel in OLQ neurons suggests that DELM channels set basal neuronal excitability. Together, our data show that DELM-1 and DELM-2 are expressed in glia associated with touch neurons where they are not needed for neuronal structural integrity or cellular distribution of neuronal sensory channels, but rather for their function.


Subject(s)
Caenorhabditis elegans Proteins/metabolism , Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Neuroglia/metabolism , Touch Perception/physiology , Touch/physiology , Animals , Caenorhabditis elegans/genetics , Caenorhabditis elegans/metabolism , Caenorhabditis elegans Proteins/genetics , Degenerin Sodium Channels/genetics , Epithelial Sodium Channels/genetics , Mechanotransduction, Cellular/physiology , Neurons/metabolism
16.
Br J Pharmacol ; 168(7): 1584-96, 2013 Apr.
Article in English | MEDLINE | ID: mdl-22928819

ABSTRACT

BACKGROUND AND PURPOSE: Chondrocytes exist within cartilage and serve to maintain the extracellular matrix. It has been postulated that osteoarthritic (OA) chondrocytes lose the ability to regulate their volume, affecting extracellular matrix production. In previous studies, we identified expression of epithelial sodium channels (ENaC) in human chondrocytes, but their function remained unknown. Although ENaC typically has Na(+) transport roles, it is also involved in the cell volume regulation of rat hepatocytes. ENaC is a member of the degenerin (Deg) family, and ENaC/Deg-like channels have a low conductance and high sensitivity to benzamil. In this study, we investigated whether canine chondrocytes express functional ENaC/Deg-like ion channels and, if so, what their function may be. EXPERIMENTAL APPROACH: Canine chondrocytes were harvested from dogs killed for unassociated welfare reasons. We used immunohistochemistry and patch-clamp electrophysiology to investigate ENaC expression and video microscopy to analyse the effects of pharmacological inhibition of ENaC/Deg on cell volume regulation. KEY RESULTS: Immunofluorescence showed that canine chondrocytes expressed ENaC protein. Single-channel recordings demonstrated expression of a benzamil-sensitive Na(+) conductance (9 pS), and whole-cell experiments show this to be approximately 1.5 nS per cell with high selectivity for Na(+) . Benzamil hyperpolarized chondrocytes by approximately 8 mV with a pD2 8.4. Chondrocyte regulatory volume decrease (RVI) was inhibited by benzamil (pD2 7.5) but persisted when extracellular Na(+) ions were replaced by Li(+) . CONCLUSION AND IMPLICATIONS: Our data suggest that benzamil inhibits RVI by reducing the influx of Na(+) ions through ENaC/Deg-like ion channels and present ENaC/Deg as a possible target for pharmacological modulation of chondrocyte volume.


Subject(s)
Amiloride/analogs & derivatives , Chondrocytes/drug effects , Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Amiloride/pharmacology , Animals , Cell Size/drug effects , Chondrocytes/cytology , Chondrocytes/metabolism , Dogs , Fluorescent Antibody Technique , In Vitro Techniques , Patch-Clamp Techniques
17.
J Gen Physiol ; 140(4): 391-402, 2012 Oct.
Article in English | MEDLINE | ID: mdl-23008433

ABSTRACT

Degenerin/epithelial Na(+) channels (DEG/ENaCs) are Na(+) channels that are blocked by the diuretic amiloride. In general, they are impermeable for Ca(2+) or have a very low permeability for Ca(2+). We describe here, however, that a DEG/ENaC from the cnidarian Hydra magnipapillata, the Hydra Na(+) channel (HyNaC), is highly permeable for Ca(2+) (P(Ca)/P(Na) = 3.8). HyNaC is directly gated by Hydra neuropeptides, and in Xenopus laevis oocytes expressing HyNaCs, RFamides elicit currents with biphasic kinetics, with a fast transient component and a slower sustained component. Although it was previously reported that the sustained component is unselective for monovalent cations, the selectivity of the transient component had remained unknown. Here, we show that the transient current component arises from secondary activation of the Ca(2+)-activated Cl(-) channel (CaCC) of Xenopus oocytes. Inhibiting the activation of the CaCC leads to a simple on-off response of peptide-activated currents with no apparent desensitization. In addition, we identify a conserved ring of negative charges at the outer entrance of the HyNaC pore that is crucial for the high Ca(2+) permeability, presumably by attracting divalent cations to the pore. At more positive membrane potentials, the binding of Ca(2+) to the ring of negative charges increasingly blocks HyNaC currents. Thus, HyNaC is the first member of the DEG/ENaC gene family with a high Ca(2+) permeability.


Subject(s)
Calcium/metabolism , Degenerin Sodium Channels/metabolism , Epithelial Sodium Channels/metabolism , Ion Channel Gating , Animals , Cell Membrane Permeability , Chloride Channels/antagonists & inhibitors , Chlorides/metabolism , Degenerin Sodium Channels/chemistry , Epithelial Sodium Channels/chemistry , Hydra , Membrane Potentials , Neuropeptides/metabolism , Nitrobenzoates/pharmacology , Protein Structure, Tertiary , Sodium Channel Agonists/metabolism , Static Electricity , Xenopus
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