ABSTRACT
La Actinomicosis Periapical es una lesión asociada con infecciones producidas por especies de Actinomyces y se ha considerado un factor en la perpetuación de las radiolucencias periapicales después de los tratamientos endodóncicos. El objetivo de esta revisión es dar a conocer la frecuencia de especies de Actinomices en la Actinomicosis Periapical...
Periapical Actinomycosis is a periapical lesion associated with infections caused by Actinomyces species and has been considered a contributing factor in the perpetuation of periapical radiolucencies after root canals. The objective of this review is to present frequency of Actinomyces species in Periapical Actinomycosis...
Subject(s)
Humans , Male , Female , Actinomycosis/physiopathology , Gram-Positive Rods/growth & development , Dental Pulp Diseases/diagnosis , Dental Pulp Diseases/virology , Soft Tissue Infections , Actinomyces , Bacteriology , Dental Pulp Cavity , Endodontics , Infectious Disease MedicineABSTRACT
INTRODUCTION: Bacterial viruses (phages) have been used successfully in the treatment of animal and human bacterial infections. This study examined the potential use of phage therapy against Pseudomonas aeruginosa strain PA14 biofilms in a root canal model. METHODS: Part 1: The 24-hour and 96-hour PA14 biofilms grown in microplates were treated with phages identified as possessing potential biofilm-degrading activities, and the post-treatment bacterial biomass was quantified by using crystal violet staining. Part 2: The 24-hour and 96-hour PA14 biofilms grown in prepared root canals of extracted human mandibular incisors were treated with phages identified with potential biofilm-degrading activities. Post-treatment intracanal samples were taken by using paper points and round burs to assess phage and bacterial counts. RESULTS: Part 1: We identified 2 phages (JBD4 and JBD44a) with putative biofilm-degrading activities. Treatment of PA14 biofilms with these phages produced a significant reduction in the mean percentage of biomass in 24-hour (P< .05) and 96-hour (P= .08) biofilms. Part 2: In 24-hour and 96-hour PA14 biofilms in a root canal model, no significant difference was found in the number of colony-forming units after phage treatment (P> .05). CONCLUSIONS: Phage application significantly reduced the biomass of 24-hour and 96-hour PA14 biofilms grown on microplates but did not produce significant reduction of 24-hour or 96-hour PA14 biofilms grown in the extracted tooth model.
