ABSTRACT
Si bien la dentina y la pulpa tienen marcadas diferencias en su composición y estructura, ambas están tan íntimamente ligadas por su origen embriológico, que cualquier cosa que afecte a la dentina lo hará sobre la pulpa y viceversa. El ejemplo más claro en ese sentido está dado por el líquido intersticial. Este líquido, semejante al plasma pero con menos proteínas, constituye una continuidad entre ambos tejidos y sus efectos hidrodinámicos son muy importantes, tanto en los estados fisiológicos como en los patológicos
Subject(s)
Humans , Dentin/anatomy & histology , Dentin/physiopathology , Dentin/chemistry , Dentin Permeability , Dentin, Secondary/physiology , Dentin/ultrastructure , Dentinal Fluid/physiology , Composite Resins/chemistry , Smear LayerABSTRACT
Si bien la dentina y la pulpa tienen marcadas diferencias en su composición y estructura, ambas están tan íntimamente ligadas por su origen embriológico, que cualquier cosa que afecte a la dentina lo hará sobre la pulpa y viceversa. El ejemplo más claro en ese sentido está dado por el líquido intersticial. Este líquido, semejante al plasma pero con menos proteínas, constituye una continuidad entre ambos tejidos y sus efectos hidrodinámicos son muy importantes, tanto en los estados fisiológicos como en los patológicos(AU)
Subject(s)
Humans , Dentin/anatomy & histology , Dentin/physiopathology , Dentin/chemistry , Dentin, Secondary/physiology , Dentinal Fluid/physiology , Smear Layer , Dentin/ultrastructure , Dentin Permeability , Composite Resins/chemistryABSTRACT
INTRODUCTION: The aim of this study was to analyze the contribution of nonresident progenitor/stem cells and hematopoietic cells to reparative dentinogenesis. METHODS: Parabiosis was established between C57BL/6-TgN(ACTbEGFP)10sb/J transgenic mice (GFP+) and C57BL/6 wild-type mice (GFP-) to ensure blood cross-circulation between animals. Reparative dentinogenesis was stimulated by pulp exposures and capping on the first maxillary molar in the GFP- mice. Histologic sections of injured molars from GFP- mice were analyzed by epifluorescence microscopy to examine the contributions of GFP+ cells (nonresident progenitor cells and hematopoietic cells originating from GFP+ mice) to reparative dentinogenesis. RESULTS: GFP+ cells were detected in close association with reparative dentin formed at the site of pulp exposure in the maxillary first molars of the GFP- mice. CONCLUSIONS: The present study suggests the participation of the nonresident progenitor cells and hematopoietic cells in reparative dentinogenesis.
Subject(s)
Dentinogenesis/physiology , Hematopoietic Stem Cells/physiology , Parabiosis/methods , Stem Cells/physiology , Acid Phosphatase/analysis , Aluminum Compounds/therapeutic use , Animals , Biomarkers/analysis , Calcium Compounds/therapeutic use , Composite Resins/chemistry , Cross Circulation/methods , Dental Materials/chemistry , Dental Pulp Capping/methods , Dental Pulp Exposure/pathology , Dental Pulp Exposure/therapy , Dentin, Secondary/physiology , Drug Combinations , Flow Cytometry , Fluorescent Dyes , Green Fluorescent Proteins , Isoenzymes/analysis , Mice , Mice, Inbred C57BL , Mice, Transgenic , Microscopy, Fluorescence , Models, Animal , Molar/pathology , Molar/physiopathology , Odontoblasts/pathology , Oxides/therapeutic use , Pulp Capping and Pulpectomy Agents/therapeutic use , Resin Cements/chemistry , Silicates/therapeutic use , Silicon Dioxide/chemistry , Tartrate-Resistant Acid Phosphatase , Zirconium/chemistryABSTRACT
OBJECTIVES: To analyse the influence of the degree of dentine mineralization on the pulp chamber temperature increase during composite light-activation. METHODS: Dentine discs (2mm thick) obtained from recently extracted teeth or those with extensive dentine sclerosis were analysed by FT-IR spectrometry in order to choose the two discs with the greatest difference in the degree of mineralization. A model tooth was set up with the dentine discs between a molar with the pulp chamber exposed and a crown with a standardized class II cavity. A K-type thermocouple was introduced into the molar root until it came into contact with the dentine discs and the cavity was filled with P60 resin composite. The temperature rise was measured for 120s after light-activation began: Standard (S) 600 mW/cm(2)/40s; Ramp (R) 0-->800 mW/cm(2)/10s+800 mW/cm(2)/10s; Boost (B) 85 0mW/cm(2)/10s and LED (L) 1.300 mW/cm(2)/40s (n=10). The same protocol was repeated after grinding the dentine discs to 1.0 and 0.5mm thickness. RESULTS: The temperature increase was significantly higher in dentine with high degree of mineralization (p<0.05). With respect to the dentine thickness, the following result was found: 2mm<1mm<0.5mm (p<0.05). The light-activation mode also presented significant difference as follows: S>R=L>B (p<0.05). CONCLUSIONS: The higher the degree of dentine mineralization the greater the increase in pulp chamber temperature. The temperature increase was influenced by the light-polymerization mode and dentine thickness.
Subject(s)
Body Temperature/physiology , Composite Resins/radiation effects , Dental Materials/radiation effects , Dental Pulp Cavity/physiology , Dentin, Secondary/physiology , Dentin/physiology , Tooth Calcification/physiology , Composite Resins/chemistry , Curing Lights, Dental , Dental Cavity Preparation , Dental Materials/chemistry , Dental Pulp/physiopathology , Dental Pulp Exposure/physiopathology , Dentin/anatomy & histology , Dentin, Secondary/anatomy & histology , Humans , Radiation Dosage , Spectroscopy, Fourier Transform Infrared , Thermometers , Time FactorsABSTRACT
A pesar del progreso logrado en el campo de la biología pulpar, la técnica y la filosofía sobre el recubrimiento pulpar ha generado inmensas controversias. Los clínicos conocen bien los éxitos logrados cuando se trabaja sobre el conducto radicular, pero no están muy convencidos de los éxitos de los recubrimientos pulpares durante actos de operatoria dental. Investigaciones recientes han demostrado que exposiciones pulpares pueden curar mediante la formación de dentina adicional y así separar la pulpa del medio externo. Es sabido, hoy en día, que el pronóstico de un recubrimiento pulpar es un tema a tratar en la operatoria dental. Los elementos que pueden producir un recubrimiento pulpar satisfactorio son discutivos en conjutno con los llamados factores de crecimiento. El propósito de crear nueva dentina es primariamente motivado por la necesidad clínica de restaurar este tejido mineralizado. La dentina rodea y protege el centro vital de la pieza dentaria que reside en la pulpa dental. Este tejido se encuentra mineralizado en un 80 por ciento y provee, en combinación con el esmalte, la mayor estructura del total de la masa dentaria. Al igual que el esmalte, la dentina es un tejido avascular pero, a diferencia de este, las células que sintetiza la dentina (los odontoblastos) pueden permanecer vitales a través de la vida adulta y volver a generar dentina adicional. Recientes investigaciones han sugerido que si los odontoblastos ubicados en la matriz preodontoblástica se pierden, es posible inducir la diferenciación de nuevas células odontoblásticas formadoras del complejo dentinopulpar utilizando las denominadas proteínas morfogenéticas. Desarrollos satisfactorios de procesos de ingeniería molecular que puedan regenerar dentina pueden tener aplicaciones clínicas como un agente para recubrimiento pulpar potenciado como desensibilizante, debido al sellado de los túbulos dentinarios (AU)
Subject(s)
Growth Substances/classification , Growth Substances/physiology , Growth Substances/therapeutic use , Dentistry, Operative/trends , Bone Morphogenetic Proteins/physiology , Odontoblasts/physiology , Dentinogenesis/physiology , Amelogenesis/physiology , Dental Pulp Capping/methods , Dentin, Secondary/physiology , Transforming Growth Factors/physiology , Transforming Growth Factors/classification , Transforming Growth Factor beta/physiology , Transforming Growth Factor alpha/physiology , Tooth Remineralization , Calcium Hydroxide/chemistry , Edetic Acid/chemistry , Dentin/anatomy & histology , Dentin/ultrastructure , Dentin/drug effectsABSTRACT
The permeability of the dentin of the pulp chamber floor of lower molars with separate roots was studied, after instrumentation of the root canals by manual or ultrasonic techniques. The dentinal permeability was evaluated by the degree of penetration of copper ions in the tissue and quantified by methods used in morphometry. None of the combinations of irrigating solution/instrumentation technique caused an increase in the permeability of dentinal tissue in the pulp chamber region, probably because the dentin is reparative dentin, which is more amorphous and less tubular than primary dentin.