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1.
Mikrochim Acta ; 191(7): 365, 2024 06 04.
Article in English | MEDLINE | ID: mdl-38831060

ABSTRACT

Copper-cobalt bimetallic nitrogen-doped carbon-based nanoenzymatic materials (CuCo@NC) were synthesized using a one-step pyrolysis process. A three-channel colorimetric sensor array was constructed for the detection of seven antioxidants, including cysteine (Cys), uric acid (UA), tea polyphenols (TP), lysine (Lys), ascorbic acid (AA), glutathione (GSH), and dopamine (DA). CuCo@NC with peroxidase activity was used to catalyze the oxidation of TMB by H2O2 at three different ratios of metal sites. The ability of various antioxidants to reduce the oxidation products of TMB (ox TMB) varied, leading to distinct absorbance changes. Linear discriminant analysis (LDA) results showed that the sensor array was capable of detecting seven antioxidants in buffer and serum samples. It could successfully discriminate antioxidants with a minimum concentration of 10 nM. Thus, multifunctional sensor arrays based on CuCo@NC bimetallic nanoenzymes not only offer a promising strategy for identifying various antioxidants but also expand their applications in medical diagnostics and environmental analysis of food.


Subject(s)
Antioxidants , Carbon , Colorimetry , Copper , Nitrogen , Nitrogen/chemistry , Colorimetry/methods , Carbon/chemistry , Antioxidants/chemistry , Antioxidants/analysis , Copper/chemistry , Cobalt/chemistry , Hydrogen Peroxide/chemistry , Humans , Catalysis , Limit of Detection , Glutathione/chemistry , Glutathione/blood , Dopamine/blood , Dopamine/analysis , Dopamine/chemistry , Benzidines/chemistry , Polyphenols/chemistry , Polyphenols/analysis , Ascorbic Acid/chemistry , Ascorbic Acid/blood , Ascorbic Acid/analysis , Oxidation-Reduction , Uric Acid/blood , Uric Acid/chemistry , Uric Acid/analysis , Cysteine/chemistry , Cysteine/blood
2.
Sci Rep ; 14(1): 13299, 2024 06 10.
Article in English | MEDLINE | ID: mdl-38858410

ABSTRACT

Radiation therapy and phototherapy are commonly used cancer treatments that offer advantages such as a low risk of adverse effects and the ability to target cancer cells while sparing healthy tissue. A promising strategy for cancer treatment involves using nanoparticles (NPs) in combination with radiation and photothermal therapy to target cancer cells and improve treatment efficacy. The synthesis of gold NPs (AuNPs) for use in biomedical applications has traditionally involved toxic reducing agents. Here we harnessed dopamine (DA)-conjugated alginate (Alg) for the facile and green synthesis of Au NPs (Au@Alg-DA NPs). Alg-DA conjugate reduced Au ions, simultaneously stabilized the resulting AuNPs, and prevented aggregation, resulting in particles with a narrow size distribution and improved stability. Injectable Au@Alg-DA NPs significantly promoted ROS generation in 4T1 breast cancer cells when exposed to X-rays. In addition, their administration raised the temperature under a light excitation of 808 nm, thus helping to destroy cancer cells more effectively. Importantly, no substantial cytotoxicity was detected in our Au@Alg-DA NPs. Taken together, our work provides a promising route to obtain an injectable combined radio enhancer and photothermally active nanosystem for further potential clinic translation.


Subject(s)
Alginates , Breast Neoplasms , Gold , Metal Nanoparticles , Gold/chemistry , Metal Nanoparticles/chemistry , Metal Nanoparticles/therapeutic use , Alginates/chemistry , Breast Neoplasms/radiotherapy , Breast Neoplasms/drug therapy , Breast Neoplasms/pathology , Female , Cell Line, Tumor , Animals , Mice , Photothermal Therapy/methods , Phototherapy/methods , Humans , Reactive Oxygen Species/metabolism , Dopamine/chemistry , Cell Survival/drug effects , Cell Survival/radiation effects
3.
ACS Appl Bio Mater ; 7(6): 3915-3931, 2024 Jun 17.
Article in English | MEDLINE | ID: mdl-38836645

ABSTRACT

One of the crucial requirements of quantum dots for biological applications is their surface modification for very specific and enhanced biological recognition and uptake. Toward this end, we present the green synthesis of bright, red-emitting carbon quantum dots derived from mango leaf extract (mQDs). These mQDs are conjugated electrostatically with dopamine to form mQDs-dopamine (mQDs:DOPA) bioconjugates. Bright-red fluorescence of mQDs was used for bioimaging and uptake in cancerous and noncancerous cell lines, tissues, and in vivo models like zebrafish. mQDs exhibited the highest uptake in brain tissue compared to the heart, kidney, and liver. mQD:DOPA conjugates killed breast cancer cells and increased uptake in epithelial RPE-1 cells and zebrafish. Additionally, mQDs:DOPA promoted neuronal differentiation of SH-SY5Y cells to differentiated neurons. Both mQDs and mQDs:DOPA exhibited the potential for higher collective cell migrations, implicating their future potential as next-generation tools for advanced biological and biomedical applications.


Subject(s)
Carbon , Cell Differentiation , Dopamine , Quantum Dots , Zebrafish , Quantum Dots/chemistry , Humans , Carbon/chemistry , Carbon/pharmacology , Dopamine/metabolism , Dopamine/chemistry , Animals , Cell Differentiation/drug effects , Neurons/drug effects , Neurons/metabolism , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Biocompatible Materials/chemical synthesis , Particle Size , Materials Testing , Antineoplastic Agents/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/chemical synthesis , Optical Imaging , Cell Survival/drug effects , Cell Line, Tumor
4.
Carbohydr Polym ; 337: 122146, 2024 Aug 01.
Article in English | MEDLINE | ID: mdl-38710570

ABSTRACT

Diabetic wounds remain a global challenge due to disordered wound healing led by inflammation, infection, oxidative stress, and delayed proliferation. Therefore, an ideal wound dressing for diabetic wounds not only needs tissue adhesiveness, injectability, and self-healing properties but also needs a full regulation of the microenvironment. In this work, adhesive wound dressings (HA-DA/PRP) with injectability were fabricated by combining platelet rich plasma (PRP) and dopamine-modified-hyaluronic acid (HA-DA). The engineered wound dressings exhibited tissue adhesiveness, rapid self-healing, and shape adaptability, thereby enhancing stability and adaptability to irregular wounds. The in vitro experiments demonstrated that HA-DA/PRP adhesives significantly promoted fibroblast proliferation and migration, attributed to the loaded PRP. The adhesives showed antibacterial properties against both gram-positive and negative bacteria. Moreover, in vitro experiments confirmed that HA-DA/PRP adhesives effectively mitigated oxidative stress and inflammation. Finally, HA-DA/PRP accelerated the healing of diabetic wounds by inhibiting bacterial growth, promoting granulation tissue regeneration, accelerating neovascularization, facilitating collagen deposition, and modulating inflammation through inducing M1 to M2 polarization, in an in vivo model of infected diabetic wounds. Overall, HA-DA/PRP adhesives with the ability to comprehensively regulate the microenvironment in diabetic wounds may provide a novel approach to expedite the diabetic wounds healing in clinic.


Subject(s)
Anti-Bacterial Agents , Diabetes Mellitus, Experimental , Hyaluronic Acid , Hydrogels , Platelet-Rich Plasma , Wound Healing , Hyaluronic Acid/chemistry , Wound Healing/drug effects , Hydrogels/chemistry , Hydrogels/pharmacology , Animals , Platelet-Rich Plasma/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Diabetes Mellitus, Experimental/drug therapy , Mice , Rats , Bandages , Male , Cell Proliferation/drug effects , Humans , Rats, Sprague-Dawley , Oxidative Stress/drug effects , Dopamine/chemistry , Fibroblasts/drug effects , Adhesives/chemistry , Adhesives/pharmacology
5.
Langmuir ; 40(22): 11635-11641, 2024 Jun 04.
Article in English | MEDLINE | ID: mdl-38775800

ABSTRACT

The presence of abnormal dopamine (DA) levels may cause serious neurological disorders, therefore, the quantitative analysis of DA and its related research are of great significance for ensuring health. Herein, the bovine serum albumin (BSA) template method has been proposed for the preparation of catalytically high-performance ruthenium dioxide/multiwalled carbon nanotube (RuO2/MWCNT) nanocomposites. The incorporation of MWCNTs has improved the active surface area and conductivity while effectively preventing the aggregation of RuO2 nanoparticles. The outstanding electrocatalytic performance of RuO2/MWCNTs has promoted the electro-oxidation of DA at neutral pH. The electrochemical sensing platform based on RuO2/MWCNTs has demonstrated a wide linear range (0.5 to 111.1 µM), low detection limit (0.167 µM), excellent selectivity, long-term stability, and good reproducibility for DA detection. The satisfactory recovery range of 94.7% to 103% exhibited by the proposed sensing podium in serum samples signifies its potential for analytical applications. The aforementioned results reveal that RuO2/MWCNT nanostructures hold promising aptitude in the electrochemical sensor to detect DA in real samples, further offering broad prospects in clinical and medical diagnosis.


Subject(s)
Biosensing Techniques , Dopamine , Electrochemical Techniques , Nanotubes, Carbon , Ruthenium Compounds , Serum Albumin, Bovine , Nanotubes, Carbon/chemistry , Dopamine/blood , Dopamine/analysis , Dopamine/chemistry , Humans , Biosensing Techniques/methods , Serum Albumin, Bovine/chemistry , Electrochemical Techniques/methods , Ruthenium Compounds/chemistry , Animals , Cattle , Limit of Detection
6.
Langmuir ; 40(20): 10718-10725, 2024 May 21.
Article in English | MEDLINE | ID: mdl-38728259

ABSTRACT

For accurate in vivo detection, nonspecific adsorption of biomacromolecules such as proteins and cells is a severe issue. The adsorption leads to electrode passivation, significantly compromising both the sensitivity and precision of sensing. Meanwhile, common antibiofouling modifications, such as polymer coatings, still grapple with issues related to biocompatibility, electrode passivation, and miniaturization. Herein, we propose a composite antibiofouling coating strategy based on zwitterionic metal-organic frameworks (Z-MOFs) and a combination of acrylamide hydrogels. On a well-designed TiO2/Z-MOF/hydrogel photoelectrode, we achieve highly sensitive and selective detection of dopamine in complex biological environments. The hydrogel's three-dimensional porous structure combined with unique microporous architecture of Z-MOF ensures effective sieving of interfering macromolecules while preserving efficient small molecules and electron transport. This innovative approach paves the way for constructing miniature, in vivo antibiofouling sensors for molecule monitoring in living organisms with complicated chemical environments.


Subject(s)
Biosensing Techniques , Dopamine , Hydrogels , Titanium , Hydrogels/chemistry , Dopamine/analysis , Dopamine/chemistry , Biosensing Techniques/methods , Titanium/chemistry , Biofouling/prevention & control , Electrochemical Techniques/methods , Photochemical Processes , Metal-Organic Frameworks/chemistry , Biocompatible Materials/chemistry , Electrodes
7.
Biosens Bioelectron ; 258: 116370, 2024 Aug 15.
Article in English | MEDLINE | ID: mdl-38744115

ABSTRACT

Protein phosphorylation is a significant post-translational modification that plays a decisive role in the occurrence and development of diseases. However, the rapid and accurate identification of phosphoproteins remains challenging. Herein, a high-throughput sensor array has been constructed based on a magnetic bimetallic nanozyme (Fe3O4@ZNP@UiO-66) for the identification and discrimination of phosphoproteins. Attributing to the formation of Fe-Zr bimetallic dual active centers, the as-prepared Fe3O4@ZNP@UiO-66 exhibits enhanced peroxidase-mimicking catalytic activity, which promotes the electron transfer from Zr center to Fe(II)/Fe(III). The catalytic activity of Fe3O4@ZNP@UiO-66 can be selectively inhibited by phosphoproteins due to the strong interaction between phosphate groups and Zr centers, as well as the ultra-robust antifouling capability of zwitterionic dopamine nanoparticle (ZNP). Considering the diverse binding affinities between various proteins with the nanozyme, the catalytic activity of Fe3O4@ZNP@UiO-66 can be changed to various degree, leading to the different absorption responses at 420 nm in the hydrogen peroxide (H2O2) - 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) system. By simply extracting different absorbance intensities at various time points, a sensor array based on reaction kinetics for the discrimination of phosphoproteins from other proteins is constructed through linear discriminant analysis (LDA). Besides, the quantitative determination of phosphoproteins and identification of protein mixtures have been realized. Further, based on the differential level of phosphoproteins in cells, the differentiation of cancer cells from normal cells can also be implemented by utilizing the proposed sensor array, showing great potential in disease diagnosis.


Subject(s)
Biosensing Techniques , Hydrogen Peroxide , Neoplasms , Phosphoproteins , Zirconium , Biosensing Techniques/methods , Humans , Phosphoproteins/chemistry , Phosphoproteins/metabolism , Hydrogen Peroxide/chemistry , Zirconium/chemistry , Peroxidase/chemistry , Dopamine/chemistry , Limit of Detection , Biomimetic Materials/chemistry , Catalysis
8.
Sensors (Basel) ; 24(9)2024 Apr 27.
Article in English | MEDLINE | ID: mdl-38732893

ABSTRACT

An abnormal level of dopamine (DA), a kind of neurotransmitter, correlates with a series of diseases, including Parkinson's disease, Willis-Ekbom disease, attention deficit hyperactivity disorder, and schizophrenia. Hence, it is imperative to achieve a precise, rapid detection method in clinical medicine. In this study, we synthesized nanocomposite carbon aerogels (CAs) doped with iron and iron carbide, based on algae residue-derived biomass materials, using Fe(NO3)3 as the iron source. The modified glassy carbon electrode (GCE) for DA detection, denoted as CAs-Fe/GCE, was prepared through surface modification with this composite material. X-ray photoelectron spectroscopy and X-ray diffraction characterization confirmed the successful doping of iron into the as-prepared CAs. Additionally, the electrochemical behavior of DA on the modified electrode surface was investigated and the results demonstrate that the addition of the CAs-Fe promoted the electron transfer rate, thereby enhancing their sensing performance. The fabricated electrochemical DA biosensor exhibits an accurate detection of DA in the concentration within the range of 0.01~200 µM, with a detection limit of 0.0033 µM. Furthermore, the proposed biosensor is validated in real samples, showing its high applicability for the detection of DA in beverages.


Subject(s)
Biosensing Techniques , Carbon , Dopamine , Electrochemical Techniques , Electrodes , Iron , Biosensing Techniques/methods , Biosensing Techniques/instrumentation , Dopamine/analysis , Dopamine/chemistry , Carbon/chemistry , Iron/chemistry , Electrochemical Techniques/methods , Gels/chemistry , Limit of Detection , Photoelectron Spectroscopy , Nanocomposites/chemistry
9.
Sensors (Basel) ; 24(9)2024 May 05.
Article in English | MEDLINE | ID: mdl-38733043

ABSTRACT

In this paper, a novel aptamer-modified nitrogen-doped graphene microelectrode (Apt-Au-N-RGOF) was fabricated and used to specifically identify and detect dopamine (DA). During the synthetic process, gold nanoparticles were loaded onto the active sites of nitrogen-doped graphene fibers. Then, aptamers were modified on the microelectrode depending on Au-S bonds to prepare Apt-Au-N-RGOF. The prepared microelectrode can specifically identify DA, avoiding interference with other molecules and improving its selectivity. Compared with the N-RGOF microelectrode, the Apt-Au-N-RGOF microelectrode exhibited higher sensitivity, a lower detection limit (0.5 µM), and a wider linear range (1~100 µM) and could be applied in electrochemical analysis fields.


Subject(s)
Aptamers, Nucleotide , Dopamine , Electrochemical Techniques , Gold , Graphite , Metal Nanoparticles , Microelectrodes , Graphite/chemistry , Dopamine/analysis , Dopamine/chemistry , Aptamers, Nucleotide/chemistry , Gold/chemistry , Electrochemical Techniques/methods , Metal Nanoparticles/chemistry , Biosensing Techniques/methods , Limit of Detection , Nitrogen/chemistry
10.
Int J Pharm ; 658: 124205, 2024 Jun 10.
Article in English | MEDLINE | ID: mdl-38734278

ABSTRACT

The current wound healing process faces numerous challenges such as bacterial infection, inflammation and oxidative stress. However, wound dressings used to promote wound healing, are not well suited to meet the clinical needs. Hyaluronic acid (HA) not only has excellent water absorption and good biocompatibility but facilitates cell function and tissue regeneration. Dopamine, on the other hand, increases the overall viscosity of the hydrogel and possesses antioxidant property. Furthermore, chitosan exhibits outstanding performance in antimicrobial, anti-inflammatory and antioxidant activities. Basic fibroblast growth factor (bFGF) is conducive to cell proliferation and migration, vascular regeneration and wound healing. Hence, we designed an all-in-one hydrogel patch containing dopamine and chitosan framed by hyaluronic acid (HDC) with sprayed gelatin methacryloyl (GelMA) microspheres loaded with bFGF (HDC-bFGF). The hydrogel patch exhibits excellent adhesive, anti-inflammatory, antioxidant and antibacterial properties. In vitro experiments, the HDC-bFGF hydrogel patch not only showed significant inhibitory effect on RAW cell inflammation and Staphylococcus aureus (S. aureus) growth but also effectively scavenged free radicals, in addition to promoting the migration of 3 T3 cells. In the mice acute infected wound model, the HDC-bFGF hydrogel patch adhered to the wound surface greatly accelerated the healing process via its anti-inflammatory and antioxidant activities, bacterial inhibition and pro-vascularization effects. Therefore, the multifunctional HDC-bFGF hydrogel patch holds great promise for clinical application.


Subject(s)
Anti-Bacterial Agents , Anti-Inflammatory Agents , Antioxidants , Chitosan , Fibroblast Growth Factor 2 , Gelatin , Hydrogels , Methacrylates , Microspheres , Staphylococcus aureus , Wound Healing , Animals , Wound Healing/drug effects , Mice , Fibroblast Growth Factor 2/administration & dosage , Fibroblast Growth Factor 2/chemistry , Fibroblast Growth Factor 2/pharmacology , Gelatin/chemistry , Staphylococcus aureus/drug effects , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Hydrogels/chemistry , Hydrogels/administration & dosage , Chitosan/chemistry , Chitosan/administration & dosage , Antioxidants/administration & dosage , Antioxidants/pharmacology , Antioxidants/chemistry , Methacrylates/chemistry , Methacrylates/administration & dosage , Anti-Inflammatory Agents/administration & dosage , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/chemistry , Male , Dopamine/administration & dosage , Dopamine/chemistry , Dopamine/pharmacology , Hyaluronic Acid/chemistry , Hyaluronic Acid/administration & dosage , Hyaluronic Acid/pharmacology , RAW 264.7 Cells , Cell Movement/drug effects , Wound Infection/drug therapy
11.
Luminescence ; 39(5): e4760, 2024 May.
Article in English | MEDLINE | ID: mdl-38738510

ABSTRACT

The present communication reports on the synthesis of a novel methyl-pyridone azo fluorescent tag (MPAFT) were proven through 1H (NMR), FT-IR, UV-vis, and high-resolution mass spectrometry. The quantum chemical parameters of MPAFT were evaluated using density functional theory (DFT) analysis. It was further investigated for its latent fingerprint (LFPs) in various surfaces and anticounterfeiting applications. By exposing Level I-Level III, ridge features to UV light with a wavelength of 365 nm, a bioimaging investigation has also demonstrated the potential of MPAFT's emission behaviour. The cyclic voltammetry (CV) and linear sweep voltammetry (LSV) at MPAFT/MGCE (modified glassy carbon electrode) were used to explore the electrochemical sensitivity and reliable detection of dopamine (DA) in neutral PBS (pH 7) electrolyte solution, and the results show good sensitivity and detection. The lower detection limit for LSV was 0.81 µM under optimum conditions.


Subject(s)
Dopamine , Electrochemical Techniques , Fluorescent Dyes , Pyrazoles , Pyridones , Pyridones/chemistry , Dopamine/analysis , Dopamine/chemistry , Fluorescent Dyes/chemistry , Fluorescent Dyes/chemical synthesis , Pyrazoles/chemistry , Humans , Molecular Structure , Density Functional Theory , Optical Imaging , Photochemical Processes
12.
Int J Biol Macromol ; 270(Pt 2): 132417, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38759857

ABSTRACT

The inflammatory response plays a critical role in standard tissue repair processes, wherein active modulation of macrophage polarization is necessary for wound healing. Dopamine, a mussel-inspired bioactive material, is widely involved in wound healing, neural/bone/myocardial regeneration, and more. Recent studies indicated that dopamine-modified biomaterials can potentially alter macrophages polarization towards a pro-healing phenotype, thereby enhancing tissue regeneration. Nevertheless the immunoregulatory activity of dopamine on macrophage polarization remains unclear. This study introduces a novel interpenetrating hydrogel to bridge this research gap. The hydrogel, combining varying concentrations of oxidized dopamine with hyaluronic acid hydrogel, allows precise regulation of mechanical properties, antioxidant bioactivity, and biocompatibility. Surprisingly, both in vivo and in vitro outcomes demonstrated that dopamine concentration modulates macrophage polarization, but not linearly. Lower concentration (2 mg/mL) potentially decrease inflammation and facilitate M2 type macrophage polarization. In contrast, higher concentration (10 mg/mL) exhibited a pro-inflammatory tendency in the late stages of implantation. RNA-seq analysis revealed that lower dopamine concentrations induced the M1/M2 transition of macrophages by modulating the NF-κB signaling pathway. Collectively, this research offers valuable insights into the immunoregulation effects of dopamine-integrated biomaterials in tissue repair and regeneration.


Subject(s)
Dopamine , Hyaluronic Acid , Hydrogels , Macrophages , Hyaluronic Acid/chemistry , Hyaluronic Acid/pharmacology , Hydrogels/chemistry , Hydrogels/pharmacology , Animals , Dopamine/pharmacology , Dopamine/chemistry , Macrophages/drug effects , Macrophages/metabolism , Mice , RAW 264.7 Cells , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Macrophage Activation/drug effects , NF-kappa B/metabolism
13.
Talanta ; 276: 126247, 2024 Aug 15.
Article in English | MEDLINE | ID: mdl-38759358

ABSTRACT

This work presents a significant investigation involving both electrochemical experiment and quantum chemical simulation approaches. The objective was to characterize the electrochemical detection of dopamine (DA). The detection was carried out using a modified carbon paste electrode (CPE) incorporating bentonite (Bent) and l-cysteine (CySH) (named as CySH/Bent/CPE). To understand and explain the oxidation mechanism of DA on the CySH/Bent modified electrode surface, the coupling of the two approaches were exploited. The CySH/Bent/CPE showed excellent electroactivity toward DA such as good sensibility, selectivity, stability, and regenerative ability. The developed sensor shows a dynamic linear range from 0.8 to 80 µM with a limit of detection and quantification of 0.5 µM and 1.5 µM, respectively. During the quantitative analysis of DA in presence of ascorbic acid (AA) and uric acid (UA) the electrochemical oxidation signals of AA, DA, and UA distinctly appear as three separate peaks. The potential differences between the peaks are 190 mv, 150 mv, and 340 mV for the AA-DA, DA-UA, and AA-UA oxidation pairs, respectively. These observations stem from square wave voltammetry (SWV) studies, along with the corresponding redox peak potential separations. The developed sensor is simple and accurate to monitor DA in human serum samples. On the other hand, CySH acts as an electrocatalyst on the CySH/Bent/CPE surface by increasing its active electron transfer sites, as suggested by the quantum chemical modeling with analytical results of Fukui. Furthermore, the voltammetric results obtained agree well with the theoretical calculations.


Subject(s)
Bentonite , Carbon , Cysteine , Dopamine , Electrochemical Techniques , Electrodes , Dopamine/blood , Dopamine/analysis , Dopamine/chemistry , Cysteine/chemistry , Cysteine/analysis , Cysteine/blood , Carbon/chemistry , Bentonite/chemistry , Electrochemical Techniques/methods , Quantum Theory , Oxidation-Reduction , Limit of Detection , Humans , Uric Acid/blood , Uric Acid/chemistry , Uric Acid/analysis
14.
Biosens Bioelectron ; 260: 116433, 2024 Sep 15.
Article in English | MEDLINE | ID: mdl-38820721

ABSTRACT

The limitations of solvent residues, unmanageable film growth regions, and substandard performance impede the extensive utilization of metal-organic framework (MOF) films for biosensing devices. Here, we report a strategy for ion design in gas-phase synthesized flexible MOF porous film to attain universal regulation of biosensing performances. The key fabrication process involves atomic layer deposition of induced layer coupled with lithography-assisted patterning and area-selective gas-phase synthesis of MOF film within a chemical vapor deposition system. Sensing platforms are subsequently formed to achieve specific detection of H2O2, dopamine, and glucose molecules by respectively implanting Co, Fe, and Ni ions into the network structure of MOF films. Furthermore, we showcase a practical device constructed from Co ions-implanted ZIF-4 film to accomplish real-time surveillance of H2O2 concentration at mouse wound. This study specifically elucidates the electronic structure and coordination mode of ion design in MOF film, and the obtained knowledge aids in tuning the electrochemical property of MOF film for advantageous sensing devices.


Subject(s)
Biosensing Techniques , Dopamine , Electrochemical Techniques , Hydrogen Peroxide , Metal-Organic Frameworks , Biosensing Techniques/methods , Metal-Organic Frameworks/chemistry , Hydrogen Peroxide/analysis , Hydrogen Peroxide/chemistry , Electrochemical Techniques/methods , Animals , Mice , Dopamine/analysis , Dopamine/chemistry , Glucose/analysis , Glucose/isolation & purification , Glucose/chemistry , Cobalt/chemistry , Nickel/chemistry , Ions/chemistry
15.
Int J Pharm ; 659: 124255, 2024 Jun 25.
Article in English | MEDLINE | ID: mdl-38782151

ABSTRACT

With the aim to find an alternative vehicle to the most used thermosensitive hydrogels for efficient nanotechnology-based nose-to-brain delivery approach for Parkinson's disease (PD) treatment, in this work we evaluated the Dopamine (DA) and the antioxidant grape seed-derived pro-anthocyanidins (Grape Seed Extract, GSE) co-loaded solid lipid nanoparticles (SLNs) put in slight viscous dispersions (SVDs). These SVDs were prepared by dispersion in water at low concentrations of mucoadhesive polymers to which SLN pellets were added. For the purpose, we investigated two polymeric blends, namely Poloxamer/Carbopol (PF-127/Carb) and oxidized alginate/Hydroxypropylmethyl cellulose (AlgOX/HPMC). Rheological studies showed that the two fluids possess Newtonian behaviour with a viscosity slightly higher that water. The pH values of the SVDs were mainly within the normal range of nasal fluid as well as almost no osmotic effect was associated to both SVDs. All the SVDs were capable to provide DA permeation through nasal porcine mucosa. Moreover, it was found that PF-127/Carb blend possesses penetration enhancer capability better than the Alg OX/HPMC combination. Flow cytometry studies demonstrated the uptake of viscous liquids incorporating fluorescent SLNs by human nasal RPMI 2650 cell in time-dependent manner. In conclusion, the SVD formulations may be considered promising alternatives to thermosensitive hydrogels strategy. Moreover, in a broader perspective, such SVD formulations may be also hopeful for treating various neurological diseases beyond PD treatment.


Subject(s)
Administration, Intranasal , Dopamine , Grape Seed Extract , Nanoparticles , Nasal Mucosa , Nanoparticles/chemistry , Grape Seed Extract/chemistry , Grape Seed Extract/administration & dosage , Animals , Viscosity , Swine , Dopamine/administration & dosage , Dopamine/chemistry , Nasal Mucosa/metabolism , Nasal Mucosa/drug effects , Humans , Poloxamer/chemistry , Drug Carriers/chemistry , Rheology , Polymers/chemistry , Lipids/chemistry , Liposomes
16.
Int J Biol Macromol ; 271(Pt 1): 132615, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38795900

ABSTRACT

A series of intricate and dynamic physiological healing processes are involved in the healing of skin wounds. Herein, a multifunctional hydrogel is firstly designed and constructed by L-arginine-grafted O-carboxymethyl chitosan (CMCA), catechol-modified oxidized hyaluronic acid (DOHA), and dopamine nanoparticles (pDA-NPs). pDA-NPs were loaded in hydrogel for inherently powerful antimicrobial properties and could be as a cross-linking agent to construct hydrogels. Raffinose (Raf) was further incorporated to obtain CMCA-DOHA-pDA2@Raf hydrogel for its function of modulating epidermal differentiation. The hydrogel has good physicochemical properties and could promote cell proliferation and migration, which shows superior hemostatic capabilities in animal models of hemorrhage. The hydrogel significantly promoted wound healing on rat skin defect models by upregulating VEGF and CD31 and decreasing IL-6 and TNF-α, stimulating neovascularization and collagen deposition in epithelial structures. This multifunctional hydrogel implies the potential to be a dynamic wound dressing.


Subject(s)
Chitosan , Dopamine , Hydrogels , Nanoparticles , Raffinose , Wound Healing , Wound Healing/drug effects , Animals , Hydrogels/chemistry , Hydrogels/pharmacology , Nanoparticles/chemistry , Dopamine/chemistry , Dopamine/pharmacology , Rats , Chitosan/chemistry , Chitosan/analogs & derivatives , Chitosan/pharmacology , Raffinose/chemistry , Raffinose/pharmacology , Cell Proliferation/drug effects , Hyaluronic Acid/chemistry , Hyaluronic Acid/pharmacology , Humans , Male , Cross-Linking Reagents/chemistry , Rats, Sprague-Dawley , Skin/drug effects , Cell Movement/drug effects
17.
Biosens Bioelectron ; 257: 116332, 2024 Aug 01.
Article in English | MEDLINE | ID: mdl-38677016

ABSTRACT

In situ detection of dopamine (DA) at single-cell level is critical for exploring neurotransmitter-related biological processes and diseases. However, the low content of DA and a variety of distractors with similar oxidation potentials as DA in cells brought great challenges. Here, a sensitive and specific electrochemical nanosensor was proposed for in situ detection of DA in single living cells based on nanodiamond (ND) and molecularly imprinted polymer (MIP)-functionalized carbon fiber nanoelectrode (ND/MIP/CFNE). Due to its excellent electrocatalytic property, ND was modified to the surface of CFNE based on amide bonding. Compared with bare CFNE, ND-modified CFNE can enhance oxidation currents of DA by about 4-fold, improving signal-to-noise ratio and detection sensitivity. MIP was further electropolymerized on the surface of nanoelectrodes to achieve specific capture and recognition of DA, which could avoid the interference of complex matrix and analogs in cells. Taking advantage of the precise positioning capability of a single-cell analyzer and micromanipulator, ND/MIP/CFNE could be precisely inserted into different locations of single cells and monitor oxidation signal of DA. The concentration of DA in the cytoplasm of single pheochromocytoma (PC12) cell was measured to be about 0.4 µM, providing a sensitive and powerful method for single-cell detection. Furthermore, the nanoelectrodes can monitor the fluctuation of intracellular DA under drug stimulation, providing new ideas and methods for new drug development and efficacy evaluation.


Subject(s)
Biosensing Techniques , Dopamine , Electrochemical Techniques , Molecularly Imprinted Polymers , Single-Cell Analysis , Dopamine/analysis , Dopamine/chemistry , Biosensing Techniques/instrumentation , Biosensing Techniques/methods , PC12 Cells , Electrochemical Techniques/methods , Molecularly Imprinted Polymers/chemistry , Animals , Rats , Nanodiamonds/chemistry , Electrodes , Carbon Fiber/chemistry , Molecular Imprinting/methods , Limit of Detection , Polymers/chemistry
18.
Anal Chem ; 96(15): 6037-6044, 2024 Apr 16.
Article in English | MEDLINE | ID: mdl-38560885

ABSTRACT

Dopamine (DA), an essential neurotransmitter, is closely associated with various neurological disorders, whose real-time dynamic monitoring is significant for evaluating the physiological activities of neurons. Electrochemical sensing methods are commonly used to determine DA, but they mostly rely on the redox reaction of its o-phenolic hydroxyl group, which makes it difficult to distinguish it from substances with this group. Here, we design a biomimetic nanozyme inspired by the coordination structure of the copper-based active site of dopamine ß-hydroxylase, which was successfully synthesized via a urea-mediated MOF pyrolysis reconstruction strategy. Experimental studies and theoretical calculations revealed that the nanozyme with Cu-N3 coordination could hydroxylate the carbon atom of the DA ß-site at a suitable potential and that the active sites of this Cu-N3 structure have the lowest binding energy for the DA ß-site. With this property, the new oxidation peak achieves the specific detection of DA rather than the traditional electrochemical signal of o-phenol hydroxyl redox, which would effectively differentiate it from neurotransmitters, such as norepinephrine and epinephrine. The sensor exhibited good monitoring capability in DA concentrations from 0.05 to 16.7 µM, and its limit of detection was 0.03 µM. Finally, the sensor enables the monitoring of DA released from living cells and can be used to quantitatively analyze the effect of polystyrene microplastics on the amount of DA released. The research provides a method for highly specific monitoring of DA and technical support for initial screening for neurocytotoxicity of pollutants.


Subject(s)
Dopamine , Mixed Function Oxygenases , Dopamine/chemistry , Phenol , Biomimetics , Copper , Plastics , Pyrolysis , Electrodes , Neurotransmitter Agents , Electrochemical Techniques/methods
19.
J Org Chem ; 89(9): 5977-5987, 2024 May 03.
Article in English | MEDLINE | ID: mdl-38557022

ABSTRACT

Mellpaladines A-C (1-3) and dopargimine (4) are dopamine-derived guanidine alkaloids isolated from a specimen of Palauan Didemnidae tunicate as possible modulators of neuronal receptors. In this study, we isolated the dopargimine derivative 1-carboxydopargimine (5), three additional mellpaladines D-F (6-8), and serotodopalgimine (9), along with a dimer of serotonin, 5,5'-dihydroxy-4,4'-bistryptamine (10). The structures of these compounds were determined based on spectrometric and spectroscopic analyses. Compound 4 and its congeners dopargine (11), nordopargimine (15), and 2-(6,7-dimethoxy-3,4-dihydroisoquinolin-1-yl)ethan-1-amine (16) were synthetically prepared for biological evaluations. The biological activities of all isolated compounds were evaluated in comparison with those of 1-4 using a mouse behavioral assay upon intracerebroventricular injection, revealing key functional groups in the dopargimines and mellpaladines for in vivo behavioral toxicity. Interestingly, these alkaloids also emerged during a screen of our marine natural product library aimed at identifying antiviral activities against dengue virus, SARS-CoV-2, and vesicular stomatitis Indiana virus (VSV) pseudotyped with Ebola virus glycoprotein (VSV-ZGP).


Subject(s)
Alkaloids , Dopamine , Urochordata , Animals , Alkaloids/chemistry , Alkaloids/pharmacology , Alkaloids/isolation & purification , Alkaloids/chemical synthesis , Urochordata/chemistry , Mice , Dopamine/chemistry , Dopamine/pharmacology , Molecular Structure , Guanidine/chemistry , Guanidine/pharmacology , Antiviral Agents/pharmacology , Antiviral Agents/chemistry , Antiviral Agents/isolation & purification , Antiviral Agents/chemical synthesis , Guanidines/chemistry , Guanidines/pharmacology , Guanidines/isolation & purification , SARS-CoV-2/drug effects , Humans
20.
Talanta ; 274: 126003, 2024 Jul 01.
Article in English | MEDLINE | ID: mdl-38569374

ABSTRACT

Antibiotics in aquatic environments raise health concerns. Therefore, the rapid, on-site, and accurate detection of antibiotic residues is crucial for protecting the environment and human health. Herein, a dumbbell-shaped iron (Fe3+)-dopamine coordination nanozyme (Fe-DCzyme) was developed via an iron-driven self-assembly strategy. It exhibited excellent peroxidase-like activity, which can be quenched by adding l-cysteine to prevent Fe3+/Fe2+ electron transfer but restored by adding norfloxacin. Given the 'On-Off-On' effect of peroxidase-like activity, Fe-DCzyme was used as a colourimetric sensor for norfloxacin detection, and showed a wide linear range from 0.05 to 6.00 µM (R2 = 0.9950) and LOD of 27.0 nM. A portable smartphone-assisted detection platform using Fe-DCzyme was also designed to convert norfloxacin-induced color changes into RGB values as well as to realise the rapid, on-site and quantitative detection of norfloxacin. A good linear relation (0.10-6.00 µM) and high sensitivity (LOD = 79.3 nM) were achieved for the smartphone-assisted Fe-DCzyme detection platform. Its application was verified using norfloxacin spiking methods with satisfactory recoveries (92.66%-119.65%). Therefore, the portable smartphone-assisted Fe-DCzyme detection platform with low cost and easy operation can be used for the rapid, on-site and visual quantitative detection of antibiotic residues in water samples.


Subject(s)
Colorimetry , Dopamine , Iron , Norfloxacin , Smartphone , Norfloxacin/analysis , Norfloxacin/chemistry , Iron/chemistry , Dopamine/analysis , Dopamine/chemistry , Colorimetry/methods , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/chemistry , Water Pollutants, Chemical/analysis , Limit of Detection , Nanostructures/chemistry
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