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1.
J Immunol ; 204(6): 1582-1591, 2020 03 15.
Article in English | MEDLINE | ID: mdl-32015010

ABSTRACT

NK cells play an important role in antiviral resistance. The integrin α2, which dimerizes with integrin ß1, distinguishes NK cells from innate lymphoid cells 1 and other leukocytes. Despite its use as an NK cell marker, little is known about the role of α2ß1 in NK cell biology. In this study, we show that in mice α2ß1 deficiency does not alter the balance of NK cell/ innate lymphoid cell 1 generation and slightly decreases the number of NK cells in the bone marrow and spleen without affecting NK cell maturation. NK cells deficient in α2ß1 had no impairment at entering or distributing within the draining lymph node of ectromelia virus (ECTV)-infected mice or at becoming effectors but proliferated poorly in response to ECTV and did not increase in numbers following infection with mouse CMV (MCMV). Still, α2ß1-deficient NK cells efficiently protected from lethal mousepox and controlled MCMV titers in the spleen. Thus, α2ß1 is required for optimal NK cell proliferation but is dispensable for protection against ECTV and MCMV, two well-established models of viral infection in which NK cells are known to be important.


Subject(s)
Ectromelia, Infectious/immunology , Herpesviridae Infections/immunology , Integrin alpha2beta1/metabolism , Killer Cells, Natural/immunology , Animals , Cell Count , Cell Proliferation , Disease Models, Animal , Ectromelia virus/immunology , Ectromelia, Infectious/blood , Ectromelia, Infectious/virology , Female , Herpesviridae Infections/blood , Herpesviridae Infections/virology , Humans , Immunity, Innate , Integrin alpha2beta1/immunology , Killer Cells, Natural/metabolism , Male , Mice , Muromegalovirus/immunology , Virus Replication/immunology
2.
Proc Natl Acad Sci U S A ; 106(35): 14984-9, 2009 Sep 01.
Article in English | MEDLINE | ID: mdl-19706459

ABSTRACT

A remarkable feature of the adaptive immune system is the speed at which small numbers of antigen-specific lymphocytes can mediate a successful immune response. Rapid expansion of T and B lymphocyte clones that have receptors specific for a particular antigen is one of the primary means by which a swift response is generated. Although much of this clonal expansion is caused by the division of antigen-specific cells, here we demonstrate an additional mechanism by which the pool of effector T cells against a viral infection can quickly enlarge. Our data show that virus-specific CD8+ cytotoxic T lymphocytes (CTL) can transfer their T cell receptors (TCR) to recipient CTL of an unrelated specificity that, as a consequence, gain the antigen specificity of the donor T cell. This process occurs within minutes via membrane exchange and results in the recipient CTL acquiring the ability to recognize and eliminate cells targeted by the donor TCR, while still retaining the antigen specificity of its own TCR. Such receptor sharing allows rapid, proliferation-independent expansion of virus-specific T cell clones of low frequency and plays a highly significant antiviral role that can protect the host from an otherwise lethal infection.


Subject(s)
Ectromelia virus/immunology , Ectromelia, Infectious/immunology , Receptors, Antigen, T-Cell/immunology , T-Lymphocytes, Cytotoxic/immunology , Animals , Cell Membrane/immunology , Cells, Cultured , Coculture Techniques , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/metabolism , Ectromelia, Infectious/blood , Epitopes/immunology , Female , Mice , Mice, Inbred C57BL , Mice, Knockout , Viral Load
3.
Comp Med ; 59(2): 180-6, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19389311

ABSTRACT

An outbreak of mousepox in a research institution was caused by Ectromelia-contaminated mouse serum that had been used for bone marrow cell culture and the cells subsequently injected into the footpads of mice. The disease initially was diagnosed by identification of gross and microscopic lesions typical for Ectromelia infection, including foci of necrosis in the liver and spleen and eosinophilic intracytoplasmic inclusion bodies in the skin. The source of infection was determined by PCR analysis to be serum obtained from a commercial vendor. To determine whether viral growth in tissue culture was required to induce viral infection, 36 mice (BALB/cJ, C57BL/6J) were experimentally exposed intraperitoneally, intradermally (footpad), or intranasally to contaminated serum or bone marrow cell cultures using the contaminated serum in the culture medium. Mice were euthanized when clinical signs developed or after 12 wk. Necropsy, PCR of spleen, and serum ELISA were performed on all mice. Mice injected with cell cultures and their cage contacts developed mousepox, antibodies to Ectromelia, and lesions, whereas mice injected with serum without cells did not. Mouse antibody production, a tool commonly used to screen biologic materials for viral contamination, failed to detect active Ectromelia contamination in mouse serum.


Subject(s)
Ectromelia virus/metabolism , Ectromelia, Infectious , Housing, Animal , Rodent Diseases , Animals , Bone Marrow Cells/cytology , Bone Marrow Cells/virology , Bone Marrow Transplantation , Cells, Cultured/virology , Disease Outbreaks , Ectromelia, Infectious/blood , Ectromelia, Infectious/diagnosis , Ectromelia, Infectious/epidemiology , Female , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Random Allocation , Rodent Diseases/blood , Rodent Diseases/diagnosis , Rodent Diseases/epidemiology , Vaccination
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