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Vet Microbiol ; 91(2-3): 197-204, 2003 Feb 02.
Article in English | MEDLINE | ID: mdl-12458168

ABSTRACT

The purpose of this study was the comparison of the diagnostic sensitivity between buffy coat (BC), peripheral blood (PB), lymph node (LN), bone marrow (BM) and short-term culture (P-D) cytology that has been based on the detection of Ehrlichia canis morulae, in the acute phase of canine monocytic ehrlichiosis (CME). Their cellular localization, total numbers and microscopic differentials were also investigated. The highest sensitivities were achieved after evaluating 1000 oil immersion fields (OIFs) in BC (66%) and an equal number in LN (60.9%) smears, separately or together (74%). The morulae were more often detected into lymphocytes than monocytes. The highest total number of morulae (n=143) were found in P-D smears. Finally, to avoid false positive diagnoses, platelets, lymphocytic azurophilic granules, lymphoglandular bodies and phagocytosed nuclear material should not be confused with the morulae.


Subject(s)
Dog Diseases/microbiology , Ehrlichia canis/isolation & purification , Ehrlichiosis/veterinary , Animals , Antibodies, Bacterial/blood , Bone Marrow/microbiology , Bone Marrow/pathology , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Dog Diseases/diagnosis , Dog Diseases/pathology , Dogs , Ehrlichia canis/cytology , Ehrlichia canis/genetics , Ehrlichiosis/blood , Ehrlichiosis/microbiology , Ehrlichiosis/pathology , Female , Fluorescent Antibody Technique, Indirect/veterinary , Lymph Nodes/microbiology , Lymph Nodes/pathology , Male , Morula/cytology , Polymerase Chain Reaction/veterinary , Sensitivity and Specificity
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