Development and characterisation of charcoal briquettes from water hyacinth (Eichhornia crassipes)-molasses blend.

Charcoal briquettes are inexpensive solid fuels made from carbonized biomass. The potential of converting water hyacinth (Eichhornia crassipes) charcoal into briquettes with molasses as binder was investigated in this study. Dried water hyacinth was carbonized at a temperature between 350°C to 500°C in a fabricated fine biomass carbonizer. A solution containing 80% by weight molasses was used in the production of briquettes having different charcoal/molasses ratios of 40:60, 30:70, and 20:80. Each briquette was characterized in terms of bulk density, calorific value, compressive strength, proximate analysis and micro-structure by Scanning Electron Microscopy. Charcoal briquettes were tested for their flammable characteristics through their burning rates and ignition time. Altering the molasses to charcoal ratio affected the quality and characteristics of the briquettes. Volatile combustible matter and fixed carbon increased with increasing amount of binder while ash content decreased. The 30:70 charcoal/molasses ratio produced the highest calorific value (16.6 MJ/kg) and compressive strength (19.1 kg/cm2). The results have shown the potential of converting water hyacinth into an alternative fuel source.

Toxicity and bioaccumulation potential of Cr (VI) and Hg (II) on differential concentration by Eichhornia crassipes in hydroponic culture.

In this work, the phytoremediation of Cr (VI) and Hg (II) ion from water by an aquatic plant Eichhornia crassipes has been studied. Plants were cultured in a double distillated water with modified Hoagland's nutrient solution at pH 6.8 supplemented with 0, 0.75, 1.50, 2.50, and 4 mg Cr/L as potassium dichromate (K(2)Cr(2)O(7)) and 0, 5, 10, 15, and 20 mg Hg/L as mercuric chloride (HgCl(2)). They were separately harvested after 3, 6 and 9 days. Plants treated with 4 mg/L of Cr (VI) accumulated the highest concentration of metal in roots (1.22 mg/g, dry weight) and shoots (0.24 mg/g, dry weight) after 9 days; while those treated with 20 mg/L of Hg (II) accumulated the highest concentration of metal in roots (4.22 mg/g, dry weight) and shoots (2.43 mg/g, dry weight) after 9 days. Eichhornia crassipes biomass was characterised using AAS, SEM and FTIR. The accumulation and relative growth of metal ions at different concentrations of chromium and mercury solution significantly increased (P<0.05) with the passage of time. The maximum values of bio-concentration factor (BCF) for Cr (VI) and Hg (II) were found to be 413.33 and 502.40 L/kg respectively.

Development of secretory cells and crystal cells in Eichhornia crassipes ramet shoot apex.

The distribution and development of secretory cells and crystal cells in young shoot apexes of water hyacinth were investigated through morphological and cytological analysis. The density of secretory cells and crystal cells were high in parenchyma tissues around the vascular bundles of shoot apexes. Three developmental stages of the secretory cells can be distinguished under transmission electron microscopy. Firstly, a large number of electron-dense vesicles formed in the cytoplasm, then fused with the tonoplast and released into the vacuole in the form of electron-dense droplets. As these droplets fused together, a large mass of dark material completely filled the vacuole. To this end, a secretion storage vacuole (SSV) formed. Secondly, an active secretion stage accompanied with degradation of the large electron-dense masses through an ill-defined autophagic process at periphery and in the limited internal regions of the SSV. Finally, after most storage substances were withdrawn, the materials remaining in the spent SSV consisted of an electron-dense network structure. The distribution and development of crystal cells in shoot apical tissue of water hyacinth were also studied by light and electron microscopy. Crystals initially formed at one site in the vacuole, where tube-like membrane structures formed crystal chambers. The chamber enlarged as the crystal grew in bidirectional manner and formed needle-shaped raphides. Most of these crystals finally occurred as raphide bundles, and the others appeared as block-like rhombohedral crystals in the vacuole. These results suggest that the formation of both secretory cells and crystal cells are involved in the metamorphosis of vacuoles and a role for vacuoles in water hyacinth rapid growth and tolerance.

Synthesis and characterization of Mn quantum dots by bioreduction with water hyacinth.

The bio-reduction method is reported as a part of a complimentary self-sustained technology, where bioremediation and metal particle production are related. The use of the characterization methods in this self sustainable technique open the expectative to be used for several other elements and with other plants, which will be discussed. However, the particular case of Mn nanoparticles involves an important option to generate nanoparticles in the range of 1-4 nanometers with a well controlled size and with a structure based on an fcc-like geometry for the smallest clusters and with more complex arrays for cluster greater than four shells, which involves magnetic moments significantly related to their atomistic configuration. At the same time, the use of the characterization methods establishes the dependence of the nanoparticle's size on the pH conditions used during the synthesis; small clusters in the range of 1-2 nm were generated using pH=5, and it was shown that for the smallest aggregates, simple polyhedron shapes are stable.

Adaptive image-processing technique and effective visualization of confocal microscopy images.

A common observation about confocal microscopy images is that lower image stacks have lower voxel intensities and are usually blurred in comparison with the upper ones. The key reasons are light absorption and scattering by the objects and particles in the volume through which light passes. This report proposes a new technique to reduce such noise impacts in terms of an adaptive intensity compensation and structural sharpening algorithm. With these image-processing procedures, effective 3D rendering techniques can be applied to faithfully visualize confocal microscopy data.