Subject(s)
Bacteria/enzymology , Bacteriological Techniques , Electrophoresis, Starch Gel/methods , Enzymes/isolation & purification , Bacteria/genetics , Bacteriological Techniques/instrumentation , Coloring Agents , Electrophoresis, Starch Gel/instrumentation , Enzymes/genetics , Solutions , Staining and Labeling/methodsSubject(s)
Electrophoresis, Polyacrylamide Gel/methods , Electrophoresis, Starch Gel/methods , Enzymes/analysis , Plant Proteins/analysis , Buffers , Electrophoresis, Polyacrylamide Gel/instrumentation , Electrophoresis, Starch Gel/instrumentation , Enzymes/isolation & purification , Enzymes/metabolism , Genetic Variation , Indicators and Reagents , Plant Proteins/isolation & purificationSubject(s)
Chromosome Mapping , Hybrid Cells/enzymology , Isoenzymes/genetics , Animals , Cell Line , Electrophoresis, Starch Gel/instrumentation , Electrophoresis, Starch Gel/methods , Humans , Hybrid Cells/cytology , Indicators and Reagents , Isoenzymes/isolation & purification , Isoenzymes/metabolism , Species SpecificityABSTRACT
The construction, the main technical details and the handling of a new electrophoretic apparatus1) are described. This system can be used for flow-through electrophoresis, as well as all slab or tube gel techniques. The suitability of the apparatus is demonstrated by the electrophoretic analysis of different samples by flow-through electrophoresis.
Subject(s)
Electrophoresis/instrumentation , Buffers , Electrophoresis, Starch Gel/instrumentation , Peptides/analysis , UltrafiltrationABSTRACT
A simple procedure for the preparation of agarose suitable for electrophoresis is developed in which anionic polysaccharides are removed by extracting the agar gel-granules with phosphate buffer (0.03 M, pH 6.8) containing urea (4 M), followed by electrophoresis in the same buffer system. Further, alkali treatment in the presence of sodium borohydride, eliminates electroendosmosis, giving essentially a neutral agarose, as judged by the electrophoretic behaviour of basic substances like crystal violet and cytochrome C. The purified agarose with yields 60-65%, has a sulphur content less than 0.1%, and forms rigid, transparent gels.
Subject(s)
Polysaccharides/isolation & purification , Sepharose/isolation & purification , Cytochrome c Group/isolation & purification , Electrophoresis, Starch Gel/instrumentation , Electrophoresis, Starch Gel/methods , Sulfur/analysisABSTRACT
A starch block electrophoresis system is described which includes (a) a single-unit Lucite electrophoresis chamber, (b) temperature control with a circulating water bath and casting resin-coated brass cooling plate, (c) continuous monitoring of block surface temperature and (d) an easily-assembled apparatus for rapid elution of protein from the starch segments.
