ABSTRACT
Results of creating the home ELISA test-system for diagnostics of enzootic encephalomyelitis (Teschen disease) has been presented. Biological components of diagnosticums has been created and described, reaction stages have been optimized. Sensitivity of the test-system for identification of antibodies to PTV-1 averaged 93%, specificity--100%. In the "sandwich"-variant the strains may be classified as serotype 1.
Subject(s)
Antibodies, Viral/analysis , Encephalomyelitis, Enzootic Porcine/diagnosis , Enteroviruses, Porcine/immunology , Animals , Encephalomyelitis, Enzootic Porcine/immunology , Enzyme-Linked Immunosorbent Assay/veterinary , Sensitivity and Specificity , Swine , Time FactorsABSTRACT
Optimal methods of purification and concentration of teschevirus strain of the pigs of the first serotype "Chernigivsky-2372" have been chosen for development of the set of Teschen pig discase diagnosticums. It has been established that differential centrifugation with following ultracentrifugation in the gradient of saccharose density permits to deprive viruses from host cell proteins, empty virus capsides and defect virus particles. That is the method fit for purification of virus preparations for the solid-phase immunoenzyme analysis.
