Effects of endurance training under calorie restriction on energy substrate metabolism in mouse skeletal muscle and liver.

We investigated whether calorie restriction (CR) enhances metabolic adaptations to endurance training (ET). Ten-week-old male Institute of Cancer Research (ICR) mice were fed ad libitum or subjected to 30% CR. The mice were subdivided into sedentary and ET groups. The ET group performed treadmill running (20-25 m/min, 30 min, 5 days/week) for 5 weeks. We found that CR decreased glycolytic enzyme activity and monocarboxylate transporter (MCT) 4 protein content, while enhancing glucose transporter 4 protein content in the plantaris and soleus muscles. Although ET and CR individually increased citrate synthase activity in the plantaris muscle, the ET-induced increase in respiratory chain complex I protein content was counteracted by CR. In the soleus muscle, mitochondrial enzyme activity and protein levels were increased by ET, but decreased by CR. It has been suggested that CR partially interferes with skeletal muscle adaptation to ET.

The Influence of a Polyphenol-Rich Red Berry Fruit Juice on Recovery Process and Leg Strength Capacity after Six Days of Intensive Endurance Exercise in Recreational Endurance Athletes.

BACKGROUND: Various nutritional strategies are increasingly used in sports to reduce oxidative stress and promote recovery. Chokeberry is rich in polyphenols and can reduce oxidative stress. Consequently, chokeberry juices and mixed juices with chokeberry content are increasingly used in sports. However, the data are very limited. Therefore, this study investigates the effects of the short-term supplementation of a red fruit juice drink with chokeberry content or a placebo on muscle damage, oxidative status, and leg strength during a six-day intense endurance protocol. METHODS: Eighteen recreational endurance athletes participated in a cross-over high intensity interval training (HIIT) design, receiving either juice or a placebo. Baseline and post-exercise assessments included blood samples, anthropometric data, and leg strength measurements. RESULTS: A significant increase was measured in muscle damage following the endurance protocol in all participants (∆ CK juice: 117.12 ± 191.75 U/L, ∆ CK placebo: 164.35 ± 267.00 U/L; p = 0.001, η2 = 0.17). No group effects were detected in exercise-induced muscle damage (p = 0.371, η2 = 0.010) and oxidative status (p = 0.632, η2 = 0.000). The reduction in strength was stronger in the placebo group, but group effects are missing statistical significance (∆ e1RM juice: 1.34 ± 9.26 kg, ∆ e1RM placebo: -3.33 ± 11.49 kg; p = 0.988, η2 = 0.000). CONCLUSION: Although a reduction in strength can be interpreted for the placebo treatment, no statistically significant influence of chokeberry could be determined. It appears that potential effects may only occur with prolonged application and a higher content of polyphenols, but further research is needed to confirm this.

Does overreaching from endurance-based training impair sleep: A systematic review and meta-analysis.

BACKGROUND: Overreaching is often linked to a deterioration in sleep quality, yet a comprehensive review is lacking. The aim of this systemic review and meta-analysis was to synthesise the literature and quantify the effect of overreaching from endurance-based training on sleep quality. METHOD: The review was conducted following the PRISMA guidelines. The final search was conducted in May 2023 using four electronic databases (Web of Science Core Collection, MEDLINE, Cochrane Central Database, SPORTDiscus). Studies were included for a qualitative review, while random-effects meta-analyses were conducted for objective and subjective sleep. RESULTS AND DISCUSSION: The search returned 805 articles. Fourteen studies were included in the systematic review; Three and eight articles were eligible for the meta-analyses (objective and subjective, respectively). On average, the overreaching protocols were sixteen days in length (6 to 28 days) and included exercise modalities such as cycling (number of studies [k] = 5), rowing (k = 4), triathlon (k = 3), running (k = 2), and swimming (k = 1). Actigraphy was the only form of objective sleep measurement used across all studies (k = 3), while various instruments were used to capture subjective sleep quality (k = 13). When comparing objective sleep quality following the overreaching intervention to baseline (or a control), there was a significant reduction in sleep efficiency (mean difference = -2.0%; 95% CI -3.2, -0.8%; Glass' Δ = -0.83; p < 0.01). In contrast, when comparing subjective sleep quality following the overreaching intervention to baseline (or a control), there was no effect on subjective sleep quality (Glass' Δ = -0.27; 95% CI -0.79, 0.25; p = 0.08). Importantly, none of the included studies were judged to have a low risk of bias. While acknowledging the need for more high-quality studies, it appears that overreaching from endurance-based training can deteriorate objective sleep without influencing the perception of sleep quality. PROTOCOL REGISTRATION: This protocol was registered in The International Prospective Register of Systematic Reviews (PROSPERO) on 21st November 2022, with the registration number CRD42022373204.

Circulating extracellular vesicle characteristics differ between men and women following 12 weeks of concurrent exercise training.

Concurrent resistance and endurance exercise training (CET) has well-studied benefits; however, inherent hormonal and genetic differences alter adaptive responses to exercise between sexes. Extracellular vesicles (EVs) are factors that contribute to adaptive signaling. Our purpose was to test if EV characteristics differ between men and women following CET. 18 young healthy participants underwent 12-weeks of CET. Prior to and following CET, subjects performed an acute bout of heavy resistance exercise (AHRET) consisting of 6 × 10 back squats at 75% 1RM. At rest and following AHRET, EVs were isolated from plasma and characteristics and miRNA contents were analyzed. AHRET elevated EV abundance in trained men only (+51%) and AHRET-induced changes were observed for muscle-derived EVs and microvesicles. There were considerable sex-specific effects of CET on EV miRNAs, highlighted by larger variation following the 12-week program in men compared to women at rest. Pathway analysis based on differentially expressed EV miRNAs predicted that AHRET and 12 weeks of CET in men positively regulates hypertrophy and growth pathways more so than in women. This report highlights sex-based differences in the EV response to resistance and concurrent exercise training and suggests that EVs may be important adaptive signaling factors altered by exercise training.

Effects and long-term outcomes of endurance versus resistance training as an adjunct to standard medication in patients with stable COPD: a multicenter randomized trial.

BACKGROUND: Comparisons between endurance training (ET) and resistance training (RT) have produced equivocal findings in chronic obstructive pulmonary disease (COPD) patients. The purpose of our study is to investigate the effectiveness and long-term outcomes of adding ET and RT to conventional medical treatment in patients with COPD. A secondary objective is to investigate the clinical improvements resulting from exercise training in patients with different disease severities. METHODS: The study was a multicenter, prospective trial in people with stable COPD. The cohort was randomized to three groups: individualized medical treatment group (MT), MT + endurance training group (MT + ET) and MT + resistance training group (MT + RT). Exercise was performed 3 times weekly over a 12-week period. The endpoints of exercise capacity, health-related quality of life, COPD symptoms, lung function, and anxiety and depression questionnaires were re-evaluated at baseline, at the completion of the intervention and at 6 and 12-month follow-up. According to the COPD assessment tool offered by GOLD guidelines, patients were stratified into GOLD A and B groups and GOLD C and D groups for further subgroup analysis. RESULTS: The intention-to-treat (ITT) population included 366 patients, 328 of them completed the study protocol over 12 months (the PP-population). There were no significant differences in the primary outcome, quality of life, between patients who underwent medical treatment (MT) alone, MT + endurance training (MT + ET), or MT + resistance training (MT + RT) at the completion of the intervention, 6-, or 12-month follow-up. Additionally, no significant differences were observed between MT, MT + RT, or MT + ET groups concerning the primary outcome, exercise capacity (3MWD), after initial 3 months of intervention. However, a small statistically significant difference was noted in favor of MT + ET compared to MT + RT at 12 months (ITT: Δ3MWD in ET vs RT = 5.53 m, 95% confidence interval: 0.87 to 13.84 m, P = 0.03) (PP: Δ3MWD in ET vs RT = 7.67 m, 95% confidence interval: 0.93 to 16.27 m, P = 0.04). For patients in the GOLD C and D groups, improvement in quality of life following ET or RT was significantly superior to medical intervention alone. Furthermore, upon completion of the exercise regimen, RT exhibited a greater improvement in anxiety compared to ET in these patients (ITT: ΔHAD-A at 3-month: RT = -1.63 ± 0.31 vs ET = -0.61 ± 0.33, p < 0.01) (PP: ΔHAD-A at 3-month: RT = -1.80 ± 0.36 vs ET = -0.75 ± 0.37, p < 0.01). CONCLUSIONS: Our study presents evidence of the beneficial effects of ET and RT in combination with standard medical treatment, as well as the long-term effects over time after the intervention. While the statistically significant effect favoring ET over RT in terms of exercise capacity was observed, it should be interpreted cautiously. Patients in severe stages of COPD may derive greater benefits from either ET or RT and should be encouraged accordingly. These findings have implications for exercise prescription in patients with COPD. TRIAL REGISTRATION: ChiCTR-INR-16009892 (17, Nov, 2016).

The effect of craniocervical flexion and neck endurance exercises plus pulmonary rehabilitation on pulmonary function in spinal cord injury: a pilot single-blinded randomised controlled trial.

STUDY DESIGN: Randomised controlled trial with computerised allocation, assessor blinding and intention-to-treat analysis. OBJECTIVE: This study wanted to prove that cervicocranial flexion exercise (CCFE) and superficial neck flexor endurance training combined with common pulmonary rehabilitation is feasible for improving spinal cord injury people's pulmonary function. SETTING: Taoyuan General Hospital, Ministry of Health and Welfare: Department of Physiotherapy, Taiwan. METHOD: Thirteen individuals who had sustained spinal cord injury for less than a year were recruited and randomised assigned into two groups. The experimental group was assigned CCFEs and neck flexor endurance training plus normal cardiopulmonary rehabilitation. The control group was assigned general neck stretching exercises plus cardiopulmonary rehabilitation. Lung function parameters such as forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), FEV1/FVC, peak expiratory flow rate (PEFR), inspiratory capacity (IC), dyspnoea, pain, and neck stiffness were recorded once a week as short-term outcome measure. RESULT: The experimental group showed significant time effects for FVC (pre-therapy: 80.4 ± 21.4, post-therapy: 86.9 ± 16.9, p = 0.021, 95% CI: 0.00-0.26) and PEFR (pre-therapy: 67.0 ± 33.4; post-therapy: 78.4 ± 26.9, p = 0.042, 95% CI: 0.00-0.22) after the therapy course. Furthermore, the experimental group showed significant time effects for BDI (experimental group: 6.3 ± 3.0; control group: 10.8 ± 1.6, p = 0.012, 95% CI: 0.00-0.21). CONCLUSION: The exercise regime for the experimental group could efficiently increase lung function due to the following three reasons: first, respiratory accessory muscle endurance increases through training. Second, posture becomes less kyphosis resulting increasing lung volume. Third, the ratio between superficial and deep neck flexor is more synchronised. IRB TRIAL REGISTRATION: TYGH108045. CLINICAL TRIAL REGISTRATION: NCT04500223.

The impact of individualised versus standardised endurance and resistance training on the fitness-fatness index in inactive adults.

OBJECTIVES: The aim of the current study was to investigate the impact of individualised versus standardised combined endurance and resistance training on the fitness-fatness index in physically inactive adults. DESIGN: Randomised controlled trial. METHODS: Fifty-four participants aged 21-55 years were randomised into three groups; 1) non-exercise control (n = 18), 2) standardised moderate-intensity continuous training (n = 18), or 3) individualised moderate-intensity continuous training + high-intensity interval training (n = 18). The fitness-fatness index was calculated by dividing cardiorespiratory fitness (expressed as metabolic equivalents) by the waist-to-height ratio. Participants were classified as likely responders to the intervention if a change of ≥1 fitness-fatness index unit was achieved. RESULTS: The individualised group showed the greatest fitness-fatness index improvement (between group difference p < 0.001), with 100 % of this group classified as likely responders, compared to the standardised (68 %) and non-exercise control (0 %) groups. CONCLUSIONS: An individualised, threshold-based exercise programme may produce more favourable changes in the fitness-fatness index than a standardised exercise programme.

Cambios enzimáticos durante el entrenamiento anaeróbico láctico y su influencia en la capacidad aeróbica / Enzymatic changes during lactic anaerobic training and their influence on aerobic capacity

Introducción: El entrenamiento deportivo es un proceso que requiere una planificación orientada a la adaptación del cuerpo a las cargas internas versus cargas externas; de allí, surge la necesidad de conocer el comportamiento de algunas enzimas musculoesqueléticas a través del control bioquímico y fisiológico del entrenamiento en el atletismo de medio fondo. Objetivo: Analizar los cambios enzimáticos de la creatinfosfoquinasa y la lactodeshidrogenasa durante el entrenamiento anaeróbico láctico y su influencia en la capacidad aeróbica en atletas de medio fondo. Método: Se utilizó el método descriptivo comparativo con una muestra de 20 sujetos aleatorizados en dos grupos: experimental y control; el análisis se realizó bajo el modelo estadístico Anova descriptiva, pruebas T y diseño de medidas repetidas con un intervalo del 95 por ciento de confianza (p<0,05). Resultados: En las variables creatinfosfoquinasa y lactodeshidrogenasa inicial y final no hubo variaciones significativas intergrupos; se observaron diferencias en la creatinfosfoquinasa en varianza (p=0,022) del orden del 97,8 por ciento; que en promedio (p=0,088) representó los niveles alcanzados del 91,2 por ciento. En las medidas repetidas no se presentaron cambios estadísticamente significativos intergrupos. Para la variable del VO2máx se aplicó la prueba T para comparar los estados inicial y final en cada grupo y hubo diferencias en ambos grupos (control p=0,002; experimental p=0,000). Conclusiones: No hubo cambios enzimáticos importantes durante los entrenamientos anaeróbico láctico y aeróbico, a su vez, el entrenamiento anaeróbico láctico si tuvo influencia en la capacidad aeróbica(AU)

Introduction: Sports training is a process that requires a planning oriented to the adaptation of the body to internal loads versus external loads; from there, arises the need to know the behavior of some musculoskeletal enzymes through the biochemical and physiological control of training in middle-distance athletics. Objective: To analyze the enzymatic changes of creatine phosphokinase and lactodehydrogenase during lactic anaerobic training and their influence on aerobic capacity in middle-distance athletes. Methods: The descriptive comparative method was used with a sample of 20 subjects randomized in two groups: experimental and control; the analysis was performed under the descriptive Anova statistical model, T-tests and repeated measures design with a 95 percent confidence interval (p<0.05). Results: In the initial and final creatine phosphokinase and lactodehydrogenase variables, there were no significant intergroup variations; differences were observed in creatine phosphokinase regarding variance or standard deviation (p=0.022) in the order of 97.8 percent; which in average (p=0.088) represented the levels reached of 91.2 percent. In the repeated measures, there were no statistically significant intergroup changes. For the VO2max variable, the T-test was applied to compare the initial and final states in each group and there were differences in both groups (control p=0.002; experimental p=0.000). Conclusions: There were no important enzymatic changes during lactic anaerobic and aerobic training, and lactic anaerobic training had an influence on aerobic capacity(AU)

Core training impacts running athletes' physical capacity / Impactos do treinamento no core sobre a capacidade física em atletas de corrida / Impacto del entrenamiento del core en la capacidad física de los atletas que corren

ABSTRACT Introduction: Core strength training focuses mainly on the muscle group and neuromuscular system of the central area of the human body to gain strength and stability for complex movements. Studies have reported the effect of core training on high-capacity athletes, but there is still a gap regarding the impacts on physical capacity in running athletes. Objective: To study the effects of strength training on athletes' core and the direct impacts on running quality. Methods: Athletes of the men's running team at the athletic training center of the Institute of Physical Education were core strength trained for 10 to 12 weeks. After the period, physical tests, according to predetermined protocols. The data were compared before, after the intervention, and after statistical treatment, and the findings were discussed according to the scientific literature. Results: After 12 weeks of training, among the five sports skills, there was only a significant difference before and after training of body lateral flexion (right side) (P<0.05), and there was no significant difference before and after training of the other four sport skills (P > 0.05). Conclusions: Through the static and dynamic exercises of core strength training, we can effectively improve the proprioception intervention in body movement so that runners can more accurately control their joints and muscles, ensuring movement and postural accuracy. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O treinamento de força do core concentra-se principalmente no grupo muscular e no sistema neuromuscular da área central do corpo humano, com o propósito de ganho de força e estabilidade para movimentos complexos. Estudos relatam o efeito do treinamento do core em atletas de alta capacidade, porém ainda há uma lacuna quanto aos impactos sobre a capacidade física em atletas de corrida. Objetivo: Estudar os efeitos resultantes do treinamento de força no core dos atletas e os impactos diretos na qualidade da corrida. Métodos: Os atletas da equipe masculina de corrida, no centro de treinamento de atletismo do Instituto de Educação Física, foram treinados com força fundamental por 10 a 12 semanas. Após o período, testes físicos, segundo protocolos pré-determinados, foram executados. Os dados foram comparados antes e após a intervenção, após o tratamento estatístico, os achados foram discutidos segundo a literatura cientifica. Resultados: Após 12 semanas de treinamento, entre as cinco habilidades esportivas, houve apenas diferença significativa antes e depois do treinamento de flexão lateral corporal (lado direito) (P<0,05), e não houve diferença significativa antes e depois do treinamento das outras quatro habilidades esportivas (P > 0,05). Conclusão: Através dos exercícios estáticos e dinâmicos do treinamento de força do core, podemos melhorar efetivamente a intervenção da propriocepção no movimento corporal, para que os corredores possam controlar com mais precisão as articulações e músculos, garantindo a precisão do movimento e postural. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El entrenamiento de la fuerza del core se concentra principalmente en el grupo muscular y en el sistema neuromuscular de la zona central del cuerpo humano, con el fin de ganar fuerza y estabilidad para los movimientos complejos. Los estudios han informado sobre el efecto del entrenamiento del tronco en los atletas de alta capacidad, pero todavía hay un vacío en cuanto a los impactos en la capacidad física en los atletas que corren. Objetivo: Estudiar los efectos resultantes del entrenamiento de fuerza en el core de los atletas y los impactos directos en la calidad de la carrera. Métodos: Los atletas del equipo masculino de atletismo, en el centro de entrenamiento de atletismo del Instituto de Educación Física, fueron entrenados con fuerza central durante 10 a 12 semanas. Tras el periodo, se ejecutaron pruebas físicas, según protocolos predeterminados. Los datos se compararon antes y después de la intervención, tras el tratamiento estadístico, los resultados se discutieron de acuerdo con la literatura científica. Resultados: Después de 12 semanas de entrenamiento, entre las cinco habilidades deportivas, sólo hubo diferencia significativa antes y después del entrenamiento de la flexión lateral del cuerpo (lado derecho) (P<0,05), y no hubo diferencia significativa antes y después del entrenamiento de las otras cuatro habilidades deportivas (P > 0,05). Conclusiones: A través de ejercicios estáticos y dinámicos de entrenamiento de la fuerza del core, podemos mejorar eficazmente la intervención de la propiocepción en el movimiento del cuerpo, de modo que los corredores puedan controlar con mayor precisión las articulaciones y los músculos, asegurando la precisión del movimiento y la postura. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Muscle injury and sports rehabilitation management in endurance training oriented to winter sports / Lesão muscular e gerenciamento da reabilitação esportiva no treinamento de resistência direcionado aos esportes de inverno / Gestión de las lesiones musculares y de la rehabilitación deportiva en el entrenamiento de resistencia dirigido a los deportes de invierno

ABSTRACT Introduction: Muscle injury in ski sports training has gradually increased, greatly impairing performance in ice and snow sports competitions. Objective: To study muscle injury and muscle movement during ice and snow sports training and the rehabilitation of muscle injuries. Methods: Thirty skiers with knee muscle injuries were selected as subjects and underwent rehabilitation training for six weeks, and the indicators were statistically evaluated. Results: The ski injuries were mainly muscle strain, muscle or ligament strain, and ligament rupture. The indices after treatment were significantly different from those before treatment (P < 0.05); compared with the three rehabilitation programs, the improvement of each index in group C was significantly different from that in the other two groups (P < 0.05), while there was no significant difference in the improvement of each index between the multi-angle isometric training treatment in group A and the proprioceptive neuromuscular stimulation technique in group B (P>0.05). Conclusion: The influence of recovery training technology on knee muscle re-education was proposed, and a rehabilitation plan for skiing was presented. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O quadro de lesão muscular no treinamento esportivo de esqui tem aumentado gradualmente, prejudicando muito o desempenho das competições esportivas de gelo e neve. Objetivo: Estudar a lesão muscular e o movimento muscular durante o treinamento esportivo no gelo e na neve, bem como a reabilitação das lesões musculares. Métodos: Trinta esquiadores com lesão muscular no joelho foram selecionados como sujeitos e submetidos a treinamento de reabilitação por um total de 6 semanas, tendo os indicadores sido avaliados estatisticamente. Resultados: Os tipos de lesões no esqui foram principalmente tensão muscular, tensão muscular ou ligamentar e ruptura ligamentar. Os índices após o tratamento foram significativamente diferentes daqueles antes do tratamento (P < 0,05); comparado com os três programas de reabilitação, a melhora de cada índice no grupo C foi significativamente diferente da dos outros dois grupos (P < 0,05), enquanto não houve diferença significativa na melhora de cada índice entre o tratamento de treinamento isométrico multiangular no grupo A e a técnica de estimulação neuromuscular proprioceptiva no grupo B (P>0,05). Conclusão: A influência da tecnologia de treinamento de recuperação na reeducação muscular do joelho foi proposta, e foi apresentado um plano de reabilitação para a prática de esqui. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El cuadro de lesiones musculares en el entrenamiento de los deportes de esquí ha ido aumentando progresivamente, lo que perjudica en gran medida el rendimiento en las competiciones de deportes de hielo y nieve. Objetivo: Estudiar las lesiones musculares y el movimiento muscular durante el entrenamiento de los deportes de hielo y nieve, así como la rehabilitación de las lesiones musculares. Métodos: Se seleccionaron como sujetos treinta esquiadores con lesiones musculares en la rodilla y se sometieron a un entrenamiento de rehabilitación durante un total de 6 semanas, y se evaluaron estadísticamente los indicadores. Resultados: Los tipos de lesiones de esquí fueron principalmente la distensión muscular, la distensión muscular o de ligamentos y la rotura de ligamentos. Los índices después del tratamiento fueron significativamente diferentes de los anteriores (P < 0,05); en comparación con los tres programas de rehabilitación, la mejora de cada índice en el grupo C fue significativamente diferente de la de los otros dos grupos (P < 0,05), mientras que no hubo diferencias significativas en la mejora de cada índice entre el tratamiento de entrenamiento isométrico multiángulo en el grupo A y la técnica de estimulación neuromuscular propioceptiva en el grupo B (P>0,05). Conclusión: Se propuso la influencia de la tecnología de entrenamiento de recuperación en la reeducación muscular de la rodilla y se presentó un plan de rehabilitación para el esquí. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

The Effects of Six Weeks of Endurance Training and CGRP Inhibition on Nrf2 and AKT Expression in the Hippocampal Tissue of Male Wistar Rats.

Purpose: To study the effects of a six-week endurance training protocol and calcitonin gene-related peptide (CGRP) inhibition on the nuclear factor erythroid 2-related factor 2 (Nrf2) and protein kinase B (PKB) or AKT expression in the hippocampal tissue of male Wistar rats. Main Methods. Building on a controlled experimental design with a posttest, 28 healthy Wistar male rats were randomly assigned to four groups (n = 7 per group), including control, control+CGRP inhibition, endurance training, and endurance training+CGRP inhibition groups. The training groups were trained for six weeks. Rats in the CGRP inhibition group received CGRP receptor antagonist daily (0.25 mg/kg) via intravenous (IV) injection. The Nrf2 and AKT (PKB) expression was measured using the real-time PCR technique. Results: In the endurance training group, Nrf2 expression in the hippocampal tissue was increased significantly more than in other groups (P < 0.05). There was also a significant increase in the AKT expression in the endurance training group compared to the control group (P = 0.048) and in the endurance training+CGRP inhibition compared to the control group (P = 0.012). In addition, there was no significant relationship between AKT (PKB) and Nrf2 (r = -0.27, n = 28, P = 0.16). Conclusion: Endurance training alone has been able to increase Nrf2 and AKT (PKB) mRNA levels in the hippocampal tissue, considering that endurance training had no significant effect on AKT and Nrf2 expression after adding to CGRP inhibition.

Respiratory muscle endurance training improves exercise performance but does not affect resting blood pressure and sleep in healthy active elderly.

PURPOSE: Ageing is associated with increased blood pressure (BP), reduced sleep, decreased pulmonary function and exercise capacity. The main purpose of this study was to test whether respiratory muscle endurance training (RMET) improves these parameters. METHODS: Twenty-four active normotensive and prehypertensive participants (age: 65.8 years) were randomized and balanced to receive either RMET (N = 12) or placebo (PLA, N = 12). RMET consisted of 30 min of volitional normocapnic hyperpnea at 60% of maximal voluntary ventilation while PLA consisted of 1 inhalation day-1 of a lactose powder. Both interventions were performed on 4-5 days week-1 for 4-5 weeks. Before and after the intervention, resting BP, pulmonary function, time to exhaustion in an incremental respiratory muscle test (incRMET), an incremental treadmill test (IT) and in a constant-load treadmill test (CLT) at 80% of peak oxygen consumption, balance, sleep at home, and body composition were assessed. Data was analyzed with 2 × 2 mixed ANOVAs. RESULTS: Compared to PLA, there was no change in resting BP (independent of initial resting BP), pulmonary function, IT performance, sleep, body composition or balance (all p > 0.05). Performance significantly increased in the incRMET (+ 6.3 min) and the CLT (+ 3.2 min), resulting in significant interaction effects (p < 0.05). CONCLUSION: In the elderly population, RMET might be used to improve respiratory and whole body endurance performance either as an adjunct to physical exercise training or as a replacement thereof for people not being able to intensively exercise even if no change in BP or sleep may be expected.

Endurance exercise ameliorates phenotypes in Drosophila models of spinocerebellar ataxias.

Endurance exercise is a potent intervention with widespread benefits proven to reduce disease incidence and impact across species. While endurance exercise supports neural plasticity, enhanced memory, and reduced neurodegeneration, less is known about the effect of chronic exercise on the progression of movement disorders such as ataxias. Here, we focused on three different types of ataxias, spinocerebellar ataxias type (SCAs) 2, 3, and 6, belonging to the polyglutamine (polyQ) family of neurodegenerative disorders. In Drosophila models of these SCAs, flies progressively lose motor function. In this study, we observe marked protection of speed and endurance in exercised SCA2 flies and modest protection in exercised SCA6 models, with no benefit to SCA3 flies. Causative protein levels are reduced in SCA2 flies after chronic exercise, but not in SCA3 models, linking protein levels to exercise-based benefits. Further mechanistic investigation indicates that the exercise-inducible protein, Sestrin (Sesn), suppresses mobility decline and improves early death in SCA2 flies, even without exercise, coincident with disease protein level reduction and increased autophagic flux. These improvements partially depend on previously established functions of Sesn that reduce oxidative damage and modulate mTOR activity. Our study suggests differential responses of polyQ SCAs to exercise, highlighting the potential for more extensive application of exercise-based therapies in the prevention of polyQ neurodegeneration. Defining the mechanisms by which endurance exercise suppresses polyQ SCAs will open the door for more effective treatment for these diseases.

Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model.

Skeletal muscle atrophy is a physiological response to disuse, aging, and disease. We compared changes in muscle mass and the transcriptome profile after short-term immobilization in a divergent model of high and low responders to endurance training to identify biological processes associated with the early atrophy response. Female rats selectively bred for high response to endurance training (HRT) and low response to endurance training (LRT; n = 6/group; generation 19) underwent 3 day hindlimb cast immobilization to compare atrophy of plantaris and soleus muscles with line-matched controls (n = 6/group). RNA sequencing was utilized to identify Gene Ontology Biological Processes with differential gene set enrichment. Aerobic training performed prior to the intervention showed HRT improved running distance (+60.6 ± 29.6%), while LRT were unchanged (-0.3 ± 13.3%). Soleus atrophy was greater in LRT vs. HRT (-9.0 ±8.8 vs. 6.2 ±8.2%; P<0.05) and there was a similar trend in plantaris (-16.4 ±5.6% vs. -8.5 ±7.4%; P = 0.064). A total of 140 and 118 biological processes were differentially enriched in plantaris and soleus muscles, respectively. Soleus muscle exhibited divergent LRT and HRT responses in processes including autophagy and immune response. In plantaris, processes associated with protein ubiquitination, as well as the atrogenes (Trim63 and Fbxo32), were more positively enriched in LRT. Overall, LRT demonstrate exacerbated atrophy compared to HRT, associated with differential gene enrichments of biological processes. This indicates that genetic factors that result in divergent adaptations to endurance exercise, may also regulate biological processes associated with short-term muscle unloading.

Effects of Combined Strength and Endurance Training on Body Composition, Physical Fitness, and Serum Hormones During a 6-Month Crisis Management Operation.

ABSTRACT: Pihlainen, K, Kyröläinen, H, Santtila, M, Ojanen, T, Raitanen, J, and Häkkinen, K. Effects of combined strength and endurance training on body composition, physical fitness, and serum hormones during a 6-month crisis management operation. J Strength Cond Res 36(9): 2361-2370, 2022-Very few studies have examined the impact of training interventions on soldier readiness during an international military operation. Therefore, the present study investigated the effects of combined strength and endurance training on body composition, physical performance, and hormonal status during a 6-month international military deployment consisting of typical peacekeeping tasks, e.g., patrolling, observation, and on-base duties. Soldiers ( n = 78) were randomly allocated to a control group (C) or one of 3 combined whole-body strength and endurance training groups with varying strength-to-endurance training emphasis (Es = 25/75%, SE = 50/50% or Se = 75/25% of strength/endurance training). Body composition, physical performance (3000-m run, standing long jump [SLJ], isometric maximal voluntary contraction of the lower [MVC lower] and upper extremities [MVC upper ], muscle endurance tests), and selected serum hormone concentrations were determined prior to training (PRE), and after 9 (MID) and 19 (POST) weeks of training. Within- and between-group changes were analyzed using linear regression models. The average combined strength and endurance training frequency of the total subject group was 3 ± 2 training sessions per week. No changes were observed in physical performance variables in the intervention groups, whereas SLJ decreased by 1.9% in C ( p < 0.05). Maximal voluntary contraction lower increased by 12.8% in the combined intervention group ( p < 0.05), and this was significantly different to C ( p < 0.05). Testosterone-to-cortisol ratio increased in SE and Se ( p < 0.05), whereas no change was observed in C. The intervention groups maintained or improved their physical performance during deployment, which is beneficial for operational readiness. However, the high interindividual variation observed in training adaptations highlights the importance of training individualization during prolonged military operations.

Long-Term Passive Leg Stretch Improves Systemic Vascular Responsiveness as Much as Single-Leg Exercise Training.

PURPOSE: The current study compared the local and systemic vascular responsiveness after small muscle mass endurance training or passive stretching training (PST). METHODS: Thirty-six sex-matched healthy participants underwent 8-wk single-leg knee extension (SLKE) (n = 12) training or PST (n = 12), or no intervention (control, n = 12). Before and after the intervention, local and systemic vascular responsiveness was assessed by Doppler ultrasound at the femoral (local effect) and brachial artery (systemic effect) during single passive leg movement and brachial flow-mediated dilation (FMD) test, respectively. RESULTS: After training, delta femoral blood flow (representing the local vascular responsiveness) increased after SLKE and PST by +54 (7)% (effect size, 2.72; P < 0.001) and +20 (2)% (effect size, 2.43; P < 0.001), respectively, albeit with a greater extent in SLKE (post-SLKE vs post-PST: +56 [8]% [effect size, 2.92; P < 0.001]). Interestingly, the %FMD (standing for the systemic effect) increased after SLKE and PST by +12 (2)% (effect size, 0.68; P < 0.001) and +11 (1)% (effect size, 0.83; P < 0.001), respectively, without any between-groups difference (P > 0.05). No changes occurred in control. CONCLUSIONS: The present findings revealed that both active and passive training modalities induced similar improvements in the brachial artery dilatation capacity, whereas the former was more effective in improving femoral artery blood flow. Passive stretching could be used in people with limited mobility to improve vascular responsiveness both at the local and systemic level and in this latter case has similar effects as small muscle mass endurance training.

Training-intensity Distribution on Middle- and Long-distance Runners: A Systematic Review.

Training-intensity distribution (TID) is considered the key factor to optimize performance in endurance sports. This systematic review aimed to: I) characterize the TID typically used by middle-and long-distance runners; II) compare the effect of different types of TID on endurance performance and its physiological determinants; III) determine the extent to which different TID quantification methods can calculate same TID outcomes from a given training program. The keywords and search strategy identified 20 articles in the research databases. These articles demonstrated differences in the quantification of the different training-intensity zones among quantification methods (i. e. session-rating of perceived exertion, heart rate, blood lactate, race pace, and running speed). The studies that used greater volumes of low-intensity training such as those characterized by pyramidal and polarized TID approaches, reported greater improvements in endurance performance than those which used a threshold TID. Thus, it seems that the combination of high-volume at low-intensity (≥ 70% of overall training volume) and low-volume at threshold and high-intensity interval training (≤ 30%) is necessary to optimize endurance training adaptations in middle-and long-distance runners. Moreover, monitoring training via multiple mechanisms that systematically encompasses objective and subjective TID quantification methods can help coaches/researches to make better decisions.

Effect of varied plyometric training in land and aquatic medium on anaerobic power of athletes / Efeito do treinamento pliométrico variado em meio terrestre e aquático na potência anaeróbia de atletas

ABSTRACT The aim of the present study was to find out the effect of varied plyometric training (land plyometric training, aqua plyometric training and weighted vest aqua-plyometric training) on anaerobic power of school athletes. A total of forty eight (n= 48) district level school athletes were randomly selected. All the subjects were divided into four equal groups: i) land plyometric training group (n=12), ii) aqua plyometric training group (n=12), iii) aqua-plyometric training with weighted vest group (n=12) and iv) control group (n=12). The varied plyometric training was intervened for fourteen weeks in the respective training groups. In the present study, anaerobic power was considered as the dependent variable, and it was measured by Margaria-Kalamen power test. To draw statistical inferences on anaerobic power among the groups in pre and post intervention conditions, ANCOVA was used, followed by Tukey's LSD post-hoc test. The significance of the means was tested at the 0.05 level. Different plyometric training groups improved significantly with respect to the control group in anaerobic power. Therefore, various plyometric training was found as an effective means for developing anaerobic power. A significant difference was also observed between the aqua-plyometric training with weighted vest group and land plyometric training group in anaerobic power. However, rest of the experimental groups didn't differ significantly. Aqua-plyometric training with weighted vest is a more effective training method than land plyometric training for improving the anaerobic power of the school athletes.

RESUMO O objetivo do presente estudo foi descobrir o efeito do treinamento pliométrico variado (treinamento pliométrico terrestre, treinamento aquapliométrico e treinamento aquapliométrico com colete ponderado) na potência anaeróbica de atletas escolares. Um total de quarenta e oito (n= 48) atletas escolares de nível distrital foram selecionados aleatoriamente. Todos os sujeitos foram divididos em quatro grupos iguais: i) grupo treinamento pliométrico terrestre (n=12), ii) grupo treinamento aquapliométrico (n=12), iii) grupo treinamento aquapliométrico com colete ponderado (n=12) e iv) grupo controle (n=12). O treino pliométrico variado foi intervencionado durante quatorze semanas nos respectivos grupos de treino. No presente estudo, a potência anaeróbia foi considerada como variável dependente e foi mensurada pelo teste de potência de Margaria-Kalamen. Para fazer inferências estatísticas sobre a potência anaeróbica entre os grupos nas condições pré e pós-intervenção, foi utilizada a ANCOVA, seguida do teste post-hoc de Tukey LSD. A significância das médias foi testada no nível 0,05. Diferentes grupos de treinamento pliométrico melhoraram significativamente em relação ao grupo controle na potência anaeróbica. Portanto, vários treinamentos pliométricos foram encontrados como um meio eficaz para o desenvolvimento da potência anaeróbica. Uma diferença significativa também foi observada entre o grupo de treinamento aquapliométrico com colete ponderado e o grupo de treinamento pliométrico terrestre na potência anaeróbica. No entanto, o resto dos grupos experimentais não diferiram significativamente. O treinamento aquapliométrico com colete ponderado é um método de treinamento mais eficaz do que o treinamento pliométrico terrestre para melhorar a potência anaeróbica dos atletas escolares.

Fallo muscular en la hipertrofia con entrenamiento de contra resistencia: una revisión sistemática / Muscle failure in hypertrophy with resistance training: a systematic review

RESUMEN Objetivo: Determinar cuáles son los efectos que tiene el fallo muscular en el desarrollo de la hipertrofia en el entrenamiento de contra resistencia. Métodos: Estudio de tipo revisión sistemática, es decir, de enfoque cualitativo y de diseño no experimental. Se realizó una búsqueda sistemática en cinco bases de datos (Pubmed, Google Académico, ScienceDirect, Scopus, Sportdiscus). Después de analizar 405 estudios, fue preciso considerar su utilidad y relevancia con respecto a la revisión, así como también la credibilidad o experiencia del autor en la temática. Resultados: Después del cribado correspondiente y la evaluación metodológica 9 estudios cumplieron con los criterios de inclusión, según lo obtenido de esta revisión la utilización del Fallo Muscular (FM) no mostró beneficios adicionales en el aumento de la masa muscular. Además, se mostró que no existen diferencias significativas cuando se comparan cargas altas y bajas utilizando esta variable. Conclusión: Se determinó que la variable volumen es más importante en desarrollos hipertróficos independientemente de si un ejercicio se ejecuta o no hacia el fallo muscular, asimismo, es más beneficioso para la hipertrofia cuando las repeticiones no se llevan al fallo muscular si no se dejan cerca de este.

ABSTRACT Objective: To determine what are the effects of muscle failure on the development of hypertrophy in counter resistance training. Method: It is a systematic review type of study, that is, with a qualitative approach and a non-experimental design. A systematic search was carried out in 5 databases (Pubmed, Google Scholar, ScienceDirect, Scopus, Sportdiscus). After analyzing 405 studies, it was necessary to consider their usefulness and relevance in respect of the review, as well as the credibility or experience of the author on the subject. Results: After the corresponding screening and methodological evaluation, 9 studies met the inclusion criteria, as obtained from this review, the use of Muscle Failure (FM) did not show additional benefits in increasing muscle mass. In addition, it was shown that there are no significant differences when comparing high and low loads using this variable. Conclusion: It was determined that the volume variable is more important in hypertrophic developments regardless of whether or not an exercise is executed towards muscle failure, it is also more beneficial for hypertrophy when repetitions do not lead to muscle failure but are close to it.