Unusual parasitoses in urban dogs: Urinary capillariosis and linguatulosis, cases report.

Parasitic diseases caused by uncommonly diagnosed parasites may pose a threat to companion animals' health in urban environments where they are least expected. The pentastomid Linguatula serrata (tongue worm) and the capillarid Pearsonema plica (syn. Capillaria plica) are parasites with an indirect life cycle, infecting both domestic and wild carnivores. The present report describes two cases: the first one of urinary capillariosis and the other of linguatulosis, in two dogs living in the urban environment of Athens, Greece. In the case of capillariosis, the dog never lived out of the city, so it was presumably infected in the urban environment. On the contrary, in the case of linguatulosis, the dog was adopted at a young age from a rural environment but remained asymptomatic for several months while living in the city. Both dogs had mild symptoms, compatible with these infections. Urinary capillariosis and linguatulosis are uncommon in owned, pet dogs, living in cities due to epizootiological characteristics, i.e. need for wildlife reservoir for P. plica and consumption of raw infected viscera for L. serrata. Different factors contribute to the fact that such infections may occur in scenarios where they are least expected. Recent studies show a progressive worldwide increase in the number of uncommon parasitoses in pet animals, that in some cases, such as linguatulosis, are of zoonotic relevance. Regular parasitological examinations and preventive antiparasitic schemes are necessary in order to treat and prevent infections in pet animals and safeguard the health of both animals and humans under the concept of One Health.

Case report of hepatic calodiosis in a rescued dog.

Calodium hepaticum (syn. Capillaria hepatica) is a nematode responsible for parasitic zoonosis that can cause granulomatous lesions in the liver. Although murids are the main hosts, C. hepaticum has been documented in a wide range of mammals. Here, we report a case of hepatic calodiosis in a 6-month-old dog that was rescued in the municipality of Cuiabá, Mato Grosso state, midwestern Brazil. Even after being attended at a Veterinary Hospital, the case resulted in animal death. Necroscopic examination revealed significant macroscopic changes in the spleen, liver, and encephalus. Microscopic examination revealed parasitic eggs, identified as Calodium cf. hepaticum, that were rounded to oval with a bilamellar cuticle, radially striated, and with bipolar plugs. Considering the difficulty to diagnose this parasite, and the existence of diverse possible hosts in Mato Grosso due to the biodiversity of the state, further investigation is required to understand the true prevalence of C. hepaticum and the importance of this nematode in public health.

Gastrointestinal and respiratory parasites in captive mammals at Rabat zoo, with the first record of Capillaria spp. in the Fennec fox (Vulpes zerda).

The aim of the present study was to broaden the understanding of parasitism in captive wild mammals in Morocco. For this purpose, an investigation on gastrointestinal and respiratory parasites in African mammals from Rabat Zoo was carried out. A total of 47 fecal samples were collected from 30 species from November 2021 to March 2022 and examined macroscopically and microscopically. Parasites were detected in 21 species at a prevalence of 70%, with a parasite positivity rate of 89% in artiodactyls, 50% in perissodactyls, 67% in both carnivores and primates. No parasitic infection was detected in proboscideans. The most frequent infection was by nematodes with a percentage of 50%, followed by both protozoa and mixed infection with a prevalence of 10%. In conclusion, the results demonstrated that parasite prevalence remains high, even in enclosed spaces like zoos. Therefore, in order to ensure animal welfare and staff safety, sanitary measures should be implemented in such facilities, including routine diagnostic tests followed by appropriate treatment.

An unexpected cause of dysuria in a cat in Poland.

BACKGROUND: Urinary tract infestation by Capillaria spp. in domestic cats is rather rare, but can cause clinical symptoms and affect behaviour. To our knowledge, this report is the first to describe a case of urinary capillariosis in a cat in Poland. CASE PRESENTATION: A female formerly stray cat aged about 1.5 years showing dysuria, stranguria, periuria and lethargy was presented at the veterinary clinic. Urinalysis revealed the presence of Capillaria plica eggs in the sediment. The cat was treated successfully with three topical doses of Broadline (Merial, Toulouse, France). CONCLUSIONS: C. plica is a nematode whose definitive hosts are carnivores, which are infected by eating earthworms (the intermediate hosts). Thus, C. plica infestation is more frequent in wild carnivores and dogs, and rare in cats. Symptomatic bladder capillariosis in cats is very rarely diagnosed and described.

High Prevalence of Intestinal Capillariasis in Chronic Diarrhea Patients in Thailand: Serological Screening Using a Rapid Lateral-Flow Immunochromatographic Assay.

Intestinal capillariasis is an emerging fish-borne helminthic disease caused by the round worm Capillaria philippinensis. Chronic infection may lead to death if the disease is misdiagnosed and inappropriate treatment is given. We used a rapid lateral-flow immunochromatographic test for screening of intestinal capillariasis in patients with chronic diarrhea. We screened 292 chronic diarrhea patients who had visited hospitals in Thailand. Sixty-six (22.6%) cases were positive according to the kit. All positive patients received mebendazole at 200 mg twice per day for 30 consecutive days or albendazole at 200 mg twice per day for 10 consecutive days. Later, stool concentration techniques, used to examine stool samples from all serologically positive individuals on three consecutive days, revealed C. philippinensis eggs, larvae, and/or adults. The kit is useful for screening and rapid diagnosis of intestinal capillariasis in chronic diarrhea patients in an endemic area for prevention of serious disease and facilitates treatment.

REVIEW OF DIOCTOPHYME RENALE: ETIOLOGY, MORPHOLOGY, BIOLOGY, ECOEPIDEMIOLOGY, PATHOGENESIS, SYMPTOMATOLOGY, DIAGNOSIS, TREATMENT, AND PREVENTION.

Dioctophyme renale has a worldwide distribution and has been diagnosed in several wild and domestic animals as well as in humans. As numerous reports in the literature exist concerning the presence of D. renale in different animal species, as well as its diagnosis, treatment, and confirmation in new geographic areas, we reviewed existing information to contribute to the knowledge of the etiology, biology, epidemiology, pathogenesis, symptoms, diagnosis, treatment, and prevention of D. renale. Results of dioctophymosis may range from asymptomatic infection to even death of the host. Diagnosis is based on data from morphology, imaging, and antibody testing, with surgical treatment being the most effective. A high potential for infection of pets exists when there is overlap with wild parasitized animals; given common risk factors for infections in humans, D. renale should be considered as having zoonotic potential.

GIANT RED KIDNEY WORM (DIOCTOPHYMA RENALE) INFECTION IN PUPPIES LESS THAN FOUR MONTHS OF AGE FROM NORTHERN CANADA.

In this article, we present 2 case reports of puppies less than 4 mo of age at the time of infection with the nematode known as giant red kidney worm (Diotophyma renale). The first puppy was prepared for an ovariohysterectomy that evolved into an exploratory laparotomy. An inflamed omentum was observed, and D. renale infection was suspected due to the puppies' place of origin. The algorithm developed by the Ontario Society for the Prevention of Cruelty to Animals for the diagnosis and treatment of D. renale infections in dogs was followed. Both puppies had a history of origin from northern Manitoba, Canada. Due to the puppies' ages, the authors suspect that the transmission of infection for both puppies could be transmammary, transplacental, or ingestion of paratenic hosts or that these cases challenge what is thought to be the current prepatent period. In addition, D. renale infections in dogs, especially from northern communities, could be common.

Occurrence of ectopic Dioctophyma renale in a Bolivian dog.

This is the first scientific report of the ectopic occurrence of Dioctophyma renale in subcutaneous tissue of a domestic dog's paw from Bolivia. An 18-month-old male dog (Pug) from Bolivia (San Matías) was examined on September 15, 2020 presenting limited mobility and apparent swelling in the left pelvic limb. After clinic evaluation, left foot edema was observed from tibio-tarsal to metatarsophalangeal joints which was more pronounced on the dorsolateral face. An incision at the site revealed serosanguinous exudation associated with a wide reddish nematode, which was identified as a female Dioctophyma renale. Epidemiological considerations were highlighted as this disease has zoonotic potential, and the infected dog was kept in a yard to which amphibians (Anura) and reptiles had access. Moreover, there is a lack of information on its occurrence in San Matías, a border city with Brazil.

Capillaria hepatica (syn. Calodium hepaticum) as a Cause of Asymptomatic Liver Mass.

Capillaria hepatica (syn. Calodium hepaticum) is a parasitic nematode of rodents, rarely infecting humans. An asymptomatic Israeli adult male with extensive travel history was diagnosed with a liver mass on routine post-thymectomy follow-up. Imaging and computer tomography (CT) guided biopsy were inconclusive. Surgical excision revealed an eosinophilic granuloma with fragments of a nematode suspected to be C. hepatica. Molecular methods verified the diagnosis, and the patient was treated empirically. This is the first case of hepatic capillariasis described in Israel, and the first to be diagnosed using molecular methods.

Giant Red Kidney Worm (Dioctophyma renale) Screening and Treatment Protocol and Aberrant Worm Migration In Dogs From Ontario and Manitoba, Canada.

The life cycle of Dioctophyma renale involves an intermediate host (oligochaete), a paratenic hosts (fish and frogs), and a definitive host (mustelids and canids). Dogs are at risk of infection with D. renale when they consume paratenic hosts infected with the larval form of D. renale. Water containing the oligochaete intermediate host cannot be disregarded as another source of infection. Infections occur mainly in the right kidney, but worms have also been found in the abdominal cavity as well as other organs. Most dogs appear asymptomatic and infections are usually noted as incidental findings on necropsy. Recently, the Ontario Society for the Prevention of Cruelty to Animals (SPCA) and Humane Society conducted transports of dogs located in northern remote communities. In 2016, some female dogs were found to be infected with D. renale upon ovariohysterectomy. In response to this discovery, we developed a screening protocol to screen for D. renale infections. In 2018, a total of 130 intact dogs were transferred from 2 northern communities in the provinces of Ontario and Manitoba. A prevalence of 7.94% (95% confidence interval 3.87-14.11%) was found from dogs from the northern communities. The screening protocol we developed provides a method of screening for dogs that are transported from communities that could be at risk of infection with D. renale.

Abdominal dioctophymosis in a domestic cat from the Peruvian rainforest confirmed morphologically and molecularly.

A case of abdominal dioctophymosis in a domestic cat was found in San Juan Bautista district, the Peruvian rainforest, in the Loreto department of Peru. The pet went to a veterinary clinic for a routine ovariohysterectomy during which a large nematode was found in the abdominal cavity. The nematode was morphologically identified as an adult female of Dioctophyme sp. A few morphological parameters, such as the vagina distance from the anterior part and the egg size, were different than D. renale. Partial sequences of the cytochrome c oxidase subunit I (cox1) and the small subunit 18S ribosomal RNA genes were compared with the references from public sequence database and showed a genetic identifies of 89.25% and 99.65% with D. renale, respectively. This is the first mitochondrial molecular analysis of a Dioctophyme specimen from South America and the results showed up to 12.5% nucleotide sequence variation in cox 1 gene of D. renale.

Urinary capillariasis: Case report of Pearsonema (syn. Capillaria) plica infection in a dog in Greece.

Pearsonema (syn. Capillaria) plica is a nematode that resides in the urinary bladder of canids, felids and mustelids (definitive hosts) and is classified in the same class as Trichuris spp. Epidemiological and clinical data on Pearsonema plica infection in domestic animals are limited. The nematode has an indirect lifecycle that involves earthworms as intermediate hosts. A six-year-old crossbred dog from Greece, presented a history of intermittent pollakiuria and hematuria. At urine analysis, P. plica eggs were found in the urine sediment. The dog was successfully treated with a double dose of milbemycin. To the best of our knowledge this is the first case of urinary capillariasis diagnosed in a domestic animal in Greece.

Dioctophyme renale (Goeze, 1782) (Nematoda, Dioctophymidae) parasitic in mammals other than humans: A comprehensive review.

A comprehensive review of the infection of mammals with the nematode Dioctophyme renale (Goeze, 1782) (Nematoda, Dioctophymidae) is presented. Mammals, including man, are the definitive hosts for this parasite. Several aspects of the infection with the parasite in mammals other than humans are critically evaluated: geographical distribution, host species recorded so far and the relative importance of the different hosts, location of parasites within the host, prevalence and intensity of the infection, diagnostic methods, pathology induced by the parasites, epidemiology and the methods of control and treatment. The authors provide an updated review about the infection, based on a extensive bibliographic search worldwide, and point out the most relevant aspects of the biology of the parasite as well as several research topics which need to be explored for a better understanding of the biology of this interesting and important parasitic nematode.

Ectopic Dioctophyme renale in the thoracic and abdominal cavities associated with renal parasitism in a dog.

Dioctophymosis is the disease caused by the nematode Dioctophyme renale, normally found parasitizing the right kidney of dogs. The absence of symptoms is frequent in parasitized animals. The surgical procedures are commonly performed to treat this disease. This work describes a case involving a canine with renal and ectopic parasitosis in the abdominal and thoracic regions. A mixed-breed female dog, approximately four months old, was diagnosed by ultrasound as for the presence of D. renale in the right kidney and abdominal and thoracic cavities. The animal underwent exploratory celiotomy, nephrectomy of the parasitized kidney, and transdiaphragmatic thoracotomy to remove the thoracic parasite, with a single abdominal surgical wound and excellent postoperative recovery. Several reports of ectopic parasitosis are found, however, the thoracic finding is unusual, and curative therapeutic transdiaphragmatic thoracotomy for dioctophymosis in dogs has not been previously described.

Report of rare case of intense parasitism by 34 specimens of Dioctophyme renale in a dog.

Dioctophymosis is caused by Dioctophyme renale, a nematode that usually affects the right kidney of carnivores. The aim of this study was to report on a case of a dog with progressive weight loss and swollen abdomen that was diagnosed as presenting dioctophymosis. The patient underwent surgical treatment through which 34 nematodes were found, of which 18 were female and 16 were male, with a maximum length of 74 centimeters. The parasites were free in the abdominal cavity and inside the right kidney, and had caused peritonitis, free fluid, severe adherences between the abdominal organs and renal perforation. Parasitic diseases with a high number of specimens of this species are uncommon in dogs. The disease progresses with an inflammatory reaction and consequent formation of adherences and granulomatous tissue. This surrounds the eggs that were eliminated in the abdominal cavity by the free parasites. This disease occurs frequently in the city of Pelotas, Rio Grande do Sul, Brazil, where the patient of this report was living. To the best of authors' knowledge, this is the case with the largest number of specimens of D. renale removed from a single dog in vivo.

Capillaria plica in Red Foxes (Vulpes vulpes) from Serbia: Epidemiology and Diagnostic Approaches to Urinary Capillariosis in Domestic Carnivores.

PURPOSE: This study aimed to determine the presence of C. plica in red foxes and to point out the possibilities of different diagnostic methods, that could be used for diagnosis of urinary capillariosis in domestic carnivores. METHODS: Seventeen red foxes from hunting grounds in the Kolubara District, Republic of Serbia, were examined in February 2019. Animals to be shot were selected based on the criteria of gamekeepers and the permission of the competent Ministry. After hunting, the foxes were examined by ultrasound and autopsied. After the necropsy, biochemical and sediment analysis of urine morphological identification of isolated adult parasites, and macroscopic/microscopic examination of the urinary bladder and kidney tissue were performed. RESULTS: Adults of C. plica were detected in 3/17 foxes (17.6%) by ultrasound imaging and in 6/17 foxes (35.3%) by necropsy examination. Parasite eggs were found in the urinary sediment of 9/17 foxes (52.9%). The predominant histopathologic changes were amyloid degeneration of the renal glomeruli and proximal tubules (8/17 foxes-47%) as well as acute cystitis (7/17 foxes-41.2%). The occurrence of C. plica was determined in 12/17 (70.6%) of the examined foxes. DISCUSSION: This study is the first record of the C. plica in red foxes in the Republic of Serbia. Diagnostic methods used in this study could make possible the early revealing of capillariosis in domestic carnivores and could provide reliable clinical and parasitological screening of suspect animals. CONCLUSION: The study presents the first report of urinary capillariosis in a fox population in Serbia. The established high prevalence of C. plica in foxes could presume its higher prevalence in domestic and wild carnivores in the future.