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5.
Biotechnol Bioeng ; 67(6): 726-36, 2000 Mar 20.
Article in English | MEDLINE | ID: mdl-10699854

ABSTRACT

Saccharomyces cerevisiae cells, Kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels. Ethanol production from glucose solutions by an immobilized preparation of S. cerevisiae was demonstrated over a total of twenty-three days, and the half-life of such a preparation was shown to be about ten days. Immobilized K. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining properties of calcium alginate gels. Calcium alginate-immobilized chloroplasts were shown to perform the Hill reaction. Some experiments with immobilized mitochondria are reported.


Subject(s)
Alginates/history , Enzymes, Immobilized/history , Kluyveromyces , Organelles , Saccharomyces cerevisiae , Glucuronic Acid/history , Hexuronic Acids/history , History, 20th Century , Kluyveromyces/ultrastructure , Saccharomyces cerevisiae/ultrastructure
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