ABSTRACT
The gene encoding antigenic site G(1) of bovine ephemeral fever virus (BEFV) was highly expressed in the host cell Escherichia coli. An indirect G(1)-ELISA with the recombinant protein as the coating antigen was established to detect antibodies against BEFV. The result revealed that the optimal concentration of the coated antigen was 0.5 microg/well and the dilution of serum was 1:20. It was optimal that sera with P/N >or= 2.2 were considered positive, P/N Subject(s)
Antibodies, Viral/analysis
, Ephemeral Fever Virus, Bovine/immunology
, Animals
, Antigens, Viral/genetics
, Cattle
, Enzyme-Linked Immunosorbent Assay/methods
, Ephemerovirus/immunology
, Sensitivity and Specificity
, Seroepidemiologic Studies
