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1.
Food Chem Toxicol ; 53: 69-74, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23220609

ABSTRACT

Benzylparaben (BzP), a type of parabens being used as a preservative agent in cosmetics, food, and pharmaceutical products, may be ingested by humans. In this study, we performed an immature uterotrophic assay using Sprague Dawley (SD) rats by intragastric administration to determine the estrogenic effects of BzP and found significant increases in uterine weight with doses of 0.16 mg/kg body weight and higher (P<0.05). The in vivo estrogenicity of BzP was supported by in vitro results from the human estrogen receptor α (hERα)-coactivator recruiting assay and in silico molecular docking analysis performed in this study. The in vitro estrogenic activity of BzP can be observed at concentrations of 1.0×10(-8) M and higher. Molecular docking analysis showed that BzP fits well into the agonist pocket of hERα. The lowest observed effect dose (LOED) (0.16 mg/kg/day) of BzP is much lower than the documented LOEDs of other parabens. Actual risk may exist for people who consume a diet high in BzP or use BzP-laden cosmetics. In addition, we tested the sensitivity of Wistar rats to 17ß-estradiol by immature uterotrophic assay, and no obvious uterotrophic response was observed in the rats given doses up to 100 µg/kg body weight.


Subject(s)
Biological Assay/methods , Estrogens/adverse effects , Parabens/adverse effects , Animals , Cosmetics/adverse effects , Dose-Response Relationship, Drug , Estradiol/adverse effects , Estrogen Receptor alpha/administration & dosage , Female , Food Additives/adverse effects , Organ Size/drug effects , Rats , Rats, Sprague-Dawley , Rats, Wistar , Uterus/drug effects
2.
J Neurosci ; 29(6): 1897-903, 2009 Feb 11.
Article in English | MEDLINE | ID: mdl-19211896

ABSTRACT

Classical estrogen receptor-signaling mechanisms involve estradiol binding to intracellular nuclear receptors [estrogen receptor-alpha (ERalpha) and estrogen receptor-beta (ERbeta)] to promote changes in protein expression. Estradiol can also exert effects within seconds to minutes, however, a timescale incongruent with genomic signaling. In the brain, estradiol rapidly potentiates stimulated dopamine release in the striatum of female rats and enhances spontaneous rotational behavior. Furthermore, estradiol rapidly attenuates the K(+)-evoked increase of GABA in dialysate. We hypothesize that these rapid effects of estradiol in the striatum are mediated by ERalpha located on the membrane of medium spiny GABAergic neurons. This experiment examined whether overexpression of ERalpha in the striatum would enhance the effect of estradiol on rotational behavior and the K(+)-evoked increase in GABA in dialysate. Ovariectomized female rats were tested for rotational behavior or underwent microdialysis experiments after unilateral intrastriatal injections of a recombinant adeno-associated virus (AAV) containing the human ERalpha cDNA (AAV.ERalpha) into the striatum; controls received either the same vector into areas outside the striatum or an AAV containing the human alkaline phosphatase gene into the striatum (AAV.ALP). Animals that received AAV.ERalpha in the striatum exhibited significantly greater estradiol-induced contralateral rotations compared with controls and exhibited behavioral sensitization of contralateral rotations induced by a low-dose of amphetamine. ERalpha overexpression also enhanced the inhibitory effect of estradiol on K(+)-evoked GABA release suggesting that disinhibition of dopamine release from terminals in the striatum resulted in the enhanced rotational behavior.


Subject(s)
Corpus Striatum/metabolism , Corpus Striatum/virology , Estradiol/physiology , Estrogen Receptor alpha/biosynthesis , Estrogen Receptor alpha/genetics , Gene Expression Regulation/physiology , Motor Activity/physiology , gamma-Aminobutyric Acid/metabolism , Animals , Corpus Striatum/physiology , Dependovirus/genetics , Estradiol/genetics , Estrogen Receptor alpha/administration & dosage , Female , Genetic Vectors/administration & dosage , Genetic Vectors/genetics , Humans , Motor Activity/genetics , Rats , Rats, Sprague-Dawley , Sexual Behavior/physiology
3.
Philos Trans A Math Phys Eng Sci ; 366(1874): 2253-63, 2008 Jul 13.
Article in English | MEDLINE | ID: mdl-18407895

ABSTRACT

Several reports over the last few years have documented the dose-response curve for steroid hormone induction of gene transcription as a modulated property of a given receptor-agonist complex that varies with the changing concentration of a variety of factors including: homologous receptor, co-activators, co-repressors and selected co-factors. In each report, the dose-response curves are sigmoidal and show an excellent fit with the curve generated by Michaelis-Menten kinetics. In addition, even the overall function of human oestrogen receptors (hERs) can show a similar graph for the determination of sex versus oestrogen compounds in reptiles. Thus, the kinetic properties of the simple bimolecular reaction of A+B-->C appear, surprisingly, to be sufficient to describe the dose-response curve of the multi-step process of steroid-regulated gene induction that involves several molecules. Any advance in explaining why the dose-response curve for steroid-regulated gene expression is sigmoidal would assist in understanding what parameters are key factors of the dose-response curve and can benefit in the design of new oestrogenic substances. We have constructed and analysed a multi-step model of hER-induced gene transcription that explains the multiple forms of a simple dose-response curve in an in vitro transcription system.


Subject(s)
Estrogen Receptor alpha/genetics , Models, Genetic , Transcriptional Activation , Animals , Cell Line , Cell-Free System , Dose-Response Relationship, Drug , Estrogen Receptor alpha/administration & dosage , Estrogen Receptor alpha/metabolism , Humans , In Vitro Techniques , Kinetics , Mathematics , Models, Statistical , Promoter Regions, Genetic , Recombinant Proteins/agonists , Recombinant Proteins/genetics , Recombinant Proteins/metabolism
4.
Hypertension ; 50(2): 432-8, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17562975

ABSTRACT

Experimental and population-based studies indicate that female gender and estrogens protect the cardiovascular system against aldosterone-induced injury. Understanding the function of estrogens in heart disease requires more precise information on the role of both estrogen receptor (ER) subtypes, ERalpha and ERbeta. Therefore, we determined whether selective activation of ERalpha or of ERbeta would confer redundant, specific, or opposing effects on cardiovascular remodeling in aldosterone salt-treated rats. The ERalpha agonist 16alpha-LE2, the ERbeta agonist 8beta-VE2, and the nonselective estrogen receptor agonist 17beta-estradiol lowered elevated blood pressure, cardiac mass, and cardiac myocyte cross-sectional areas, as well as increased perivascular collagen accumulation and vascular osteopontin expression in ovariectomized rats receiving chronic aldosterone infusion plus a high-salt diet for 8 weeks. Uterus atrophy was prevented by 16alpha-LE2 and 17beta-estradiol but not by 8beta-VE2. Cardiac proteome analyses by 2D gel electrophoresis, mass spectrometry, and peptide sequencing identified specific subsets of proteins involved in cardiac contractility, energy metabolism, cellular stress response and extracellular matrix formation that were regulated in opposite directions by aldosterone salt treatment and by different estrogen receptor agonists. We conclude that activation of either ERalpha or ERbeta protects the cardiovascular system against the detrimental effects of aldosterone salt treatment and confers redundant, as well as specific, effects on cardiac protein expression. Nonfeminizing ERbeta agonists such as 8beta-VE2 have a therapeutic potential in the treatment of hypertensive heart disease.


Subject(s)
Estrogen Receptor alpha/administration & dosage , Estrogen Receptor beta/administration & dosage , Ventricular Remodeling/drug effects , Aldosterone/pharmacology , Analysis of Variance , Animals , Cardiovascular Physiological Phenomena , Disease Models, Animal , Dose-Response Relationship, Drug , Electrophoresis, Gel, Two-Dimensional , Female , Immunohistochemistry , Ovariectomy , Probability , Random Allocation , Rats , Rats, Wistar , Reference Values , Sensitivity and Specificity , Sodium Chloride, Dietary/administration & dosage , Ventricular Remodeling/physiology
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