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1.
Article in English | MEDLINE | ID: mdl-32811422

ABSTRACT

AIM: Favism is characterized as acute anemia, due to Glucose-6-phosphate dehydrogenase (G6PD) deficiency as a result of fava beans intake. It is associated with paleness, jaundice, and hemoglobinuria. In this study, signs, symptoms and therapeutic findings of the patients with hemolysis due to G6PD deficiency were investigated in Shahid Madani Hospital of Khorramabad, Lorestan. METHODS: This is a single-center cross-sectional descriptive study that was conducted on all children with G6PD deficiency-induced hemolysis. RESULTS: 308 children (64.3% male and 35.7% female) were included in this study. The most common complaint was jaundice (82.5%) and the most common cause of hemolysis was the intake of fava bean (85.7%). 68% of the children were treated with hydration/fluid therapy. Blood transfusion was conducted in 36.36% of the cases and the mean of blood administered was 18.9 cc/kg. CONCLUSION: In this study, hydration therapy was performed in most of the children presenting favism. Also, the incorrect calculation of the amount of blood needed for transfusion increased the frequency of blood transfusions and prolonged hospitalization time.


Subject(s)
Favism/blood , Favism/diagnosis , Glucosephosphate Dehydrogenase Deficiency/blood , Glucosephosphate Dehydrogenase Deficiency/diagnosis , Vicia faba/adverse effects , Blood Transfusion/methods , Child , Child, Preschool , Cross-Sectional Studies , Favism/epidemiology , Favism/therapy , Female , Fluid Therapy/methods , Glucosephosphate Dehydrogenase Deficiency/epidemiology , Glucosephosphate Dehydrogenase Deficiency/therapy , Humans , Infant , Male
2.
J Sci Food Agric ; 98(13): 5105-5111, 2018 Oct.
Article in English | MEDLINE | ID: mdl-29611201

ABSTRACT

BACKGROUND: Faba bean (Vicia faba) vicine and convicine (V-C) aglycones (divicine and isouramil respectively) provoke an acute hemolytic anemia called favism in individuals with a glucose-6-phosphate dehydrogenase (G6PD) enzyme defect in their red blood cells. Geneticists/plant breeders are working with faba bean to decrease V-C levels to improve public acceptance of this high-protein pulse crop. Here, we present a fast and simple ex vivo in vitro bioassay for V-C toxicity testing of faba bean or faba bean food products. RESULTS: We have shown that 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU)-treated (i.e., sensitized) normal red blood cells, like G6PD-defective blood, displayed (i) continuous glutathione (GSH) depletion with no regeneration as incubation time and the dose of aglycones increased, (ii) progressive accumulation of denatured hemoglobin products into high molecular weight (HMW) proteins with increased aglycone dose, (iii) both band 3 membrane proteins and hemichromes, in HMW protein aggregates. We have also demonstrated that sensitized red blood cells can effectively differentiate various levels of toxicity among faba bean varieties through the two hemolysis biomarkers: GSH depletion and HMW clumping. CONCLUSION: BCNU-sensitized red blood cells provide an ideal model for favism blood, to assess and compare the toxicity of faba bean varieties and their food products. © 2018 Society of Chemical Industry.


Subject(s)
Biological Assay/methods , Glucosides/analysis , Pyrimidinones/analysis , Uridine/analogs & derivatives , Vicia faba/chemistry , Erythrocytes/chemistry , Erythrocytes/drug effects , Erythrocytes/enzymology , Favism/blood , Favism/enzymology , Glucosephosphate Dehydrogenase/chemistry , Glucosides/toxicity , Hemolysis/drug effects , Humans , Pyrimidinones/toxicity , Uridine/analysis , Uridine/toxicity , Vicia faba/toxicity
6.
Eur Rev Med Pharmacol Sci ; 15(11): 1270-81, 2011 Nov.
Article in English | MEDLINE | ID: mdl-22195359

ABSTRACT

OBJECTIVE: The aim of this study was to evaluate the protective effect of Diospyros lotus L. fruit extract against the hemolytic damage induced by Vicia faba beans extract in both G6PD enzyme-deficient human and rat erythrocyte in vitro and in vivo. MATERIALS AND METHODS: In the former model, venous blood samples were obtained from five subjects with known G6PD deficiency and erythrocyte hemolysis induced by Vicia faba L. bean extract was asessed spectrophotometrically in the presence and absence of Diospyros lotus L. fruits extract. In the in vivo model, G6PD-deficient rats (induced by intraperitoneal injection of dehydroepiandrosterone for 35 days) pre-treated with different doses of Diospyros lotus L. (500, 750, 1000, and 1500 mg/kg, p.o for 7 days) were challenged with Vicia faba beans extract and the protective effect of the fruit extract against hemolysis was evaluated as above. RESULTS AND CONCLUSIONS: The results have shown that Diospyros lotus L. fruits extract has antioxidant activity that may protect against hemolytic damage induced by Vicia faba bean extract in both G6PD-deficient human and rat erythrocytes. The study gives a scientific basis for the efficacy of the fruit extract as used in Iran. The fact that this was shown in human erythrocytes in vitro is significant and provides a rationale for further testing in vivo in G6PD-deficient human populations.


Subject(s)
Diospyros/chemistry , Erythrocytes/drug effects , Erythrocytes/enzymology , Favism/blood , Favism/prevention & control , Glycogen Storage Disease Type I/blood , Glycogen Storage Disease Type I/drug therapy , Hemolysis/drug effects , Animals , Antioxidants/analysis , Biphenyl Compounds , Dose-Response Relationship, Drug , Free Radical Scavengers/metabolism , Fruit/chemistry , Hematocrit , Hemoglobinometry , Humans , In Vitro Techniques , Male , Picrates , Plant Extracts/pharmacology , Rats , Rats, Wistar
7.
Anaesthesist ; 55(5): 550-4, 2006 May.
Article in German | MEDLINE | ID: mdl-16485110

ABSTRACT

Glucose-6-phosphate dehydrogenase (G6PD) deficiency, a frequent congenital human enzyme defect, is the most frequent cause of hemolytic anemia triggered by drugs or infectious diseases. Drugs which induce acute hemolysis in patients with G6PD deficiency are often used in anesthesia and perioperative pain therapy. Considering the fact that patients from geographic regions with a high prevalence of the disease are often treated in European hospitals, special attention should be paid to this problem. We report a case of a 30-year-old female patient with favism and review the disease and anesthesia-related implications.


Subject(s)
Anesthesia , Favism/complications , Glucosephosphate Dehydrogenase Deficiency/complications , Adult , Anesthetics/adverse effects , Diagnosis, Differential , Favism/blood , Favism/genetics , Female , Glucosephosphate Dehydrogenase Deficiency/blood , Glucosephosphate Dehydrogenase Deficiency/genetics , Glutathione/metabolism , Hemolysis/drug effects , Humans , Preanesthetic Medication , Thyroidectomy
8.
Cell Physiol Biochem ; 16(4-6): 133-46, 2005.
Article in English | MEDLINE | ID: mdl-16301814

ABSTRACT

Band 3 modifications that normally occur during physiological red blood cell (RBC) senescence in humans, and occasionally in pathological conditions are described in the context of their role in enhancing RBC recognition and phagocytic removal. Band 3 modifications are mostly due to oxidative insults that gradually accumulate during the RBC lifespan or impact massively in a shorter time period in pathological conditions. The oxidative insults that impact on the RBC, the protective mechanisms that counteract those damages and the phenotypic modifications that accumulate during the RBC lifespan are described. It is shown how specific oxidative as well as non-oxidative band 3 modifications enhance RBC membrane affinity for normally circulating anti-band 3 antibodies, and how membrane-bound anti-band 3 antibodies bring about a limited complement activation and membrane deposition of complement C3 fragments. The partially covalent complexes between anti-band 3 antibodies and complement C3 fragments are very powerful opsonins readily recognized by the CR1 complement receptor on the phagocyte. Band 3 modifications typically encountered in old RBCs have crystallized to a number of band 3-centered models of RBC senescence. One of those band 3-centered models, the so-called 'band 3/complement RBC removal model' first put up by Lutz et al. is discussed in more detail. Finally, it is shown how the genetic deficiency of glucose-6-phosphate dehydrogenase (G6PD) plus fava bean consumption, and a widespread RBC parasitic disease, P. falciparum malaria, may lead to massive and rapid destruction of RBCs by a mechanism comparable to a dramatic, time-compressed enhancement of normal RBC senescence.


Subject(s)
Anion Exchange Protein 1, Erythrocyte/metabolism , Erythrocyte Aging , Erythrocytes/metabolism , Anion Exchange Protein 1, Erythrocyte/chemistry , Complement C3b/metabolism , Erythrocyte Aging/immunology , Erythrocytes/immunology , Erythrocytes/parasitology , Favism/blood , Glucosephosphate Dehydrogenase/metabolism , Glucosephosphate Dehydrogenase Deficiency/blood , Heme/metabolism , Hemeproteins/metabolism , Humans , Iron/blood , Iron/metabolism , Malaria, Falciparum/blood , Models, Biological , Receptors, Complement 3b/metabolism
9.
Toxicol Sci ; 51(2): 310-6, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10543033

ABSTRACT

Favism is an acute hemolytic anemia known to occur in susceptible individuals who ingest fava beans. Susceptibility to favism is conferred by a genetic deficiency in erythrocytic glucose-6-phosphate dehydrogenase (G6PD) activity. Although the fava bean pyrimidine aglycones, divicine and isouramil, have been implicated in the onset of favism in humans, the lack of a well-defined experimental animal model for favism has hampered progress in elucidating the mechanism underlying hemotoxicity. We have examined whether a favic-like response could be provoked in G6PD-normal rats treated with synthetic divicine. Intraperitoneal administration of divicine to rats preloaded with 51Cr-tagged erythrocytes resulted in a severe, dose-dependent decrease in blood radioactivity (TD50 approximately 0.5 mmol/kg) within 24 h. The increased rate of removal of blood radioactivity was accompanied by a rapid decline in reduced glutathione levels in the blood, decreased hematocrits, marked hemoglobinuria, splenic enlargement, and reticulocytosis. In vitro exposure of 51Cr-tagged red cells to divicine before their re-administration to isologous rats also resulted in a sharp, concentration-dependent decrease in erythrocyte survival in vivo (TC50 approximately 1.5 mM), and these divicine-damaged red cells were removed from the circulation by the spleen. These data demonstrate that a favic response can be induced in G6PD-normal rats treated with divicine, and that hemolytic activity can be reproduced in isolated red cells under conditions that will allow a direct examination of the mechanism underlying this hemotoxicity.


Subject(s)
Favism/chemically induced , Pyrimidinones/toxicity , Animals , Chromium/blood , Chromium/urine , Chromium Radioisotopes , Dose-Response Relationship, Drug , Erythrocytes/enzymology , Fabaceae , Favism/blood , Favism/enzymology , Glucosephosphate Dehydrogenase/blood , Glutathione/blood , Hemoglobins/metabolism , Hemolysis , Lethal Dose 50 , Male , Plants, Medicinal , Plants, Toxic/toxicity , Rats , Rats, Sprague-Dawley
10.
Blood ; 88(3): 1084-8, 1996 Aug 01.
Article in English | MEDLINE | ID: mdl-8704218

ABSTRACT

The endemic occurrence of favism in certain Mediterranean regions provided an investigative opportunity for testing in vivo the validity of claims as to the role of catalase in protecting human erythrocytes against peroxidative injury. Reduced activity of catalase was found in the erythrocytes of six boys who were deficient in erythrocytic glucose-6-phosphate dehydrogenase (G6PD) and who were studied while suffering hemolysis after ingesting fava beans. Activity of catalase was further reduced when their red blood cells were incubated with aminotriazole. In contrast, minimal reduction of catalase activity was found, both with and without incubation with aminotriazole, in erythrocytes of a G6PD-deficient boy who had ingested fava beans 7 days earlier and in erythrocytes of seven G6PD-deficient men with a past history of favism. These results confirmed earlier studies in vitro indicating that catalase is a major disposer of hydrogen peroxide in human erythrocytes and, like the glutathione peroxidase/reductase pathway, is dependent on the availability of reduced nicotinamide adenine dinucleotide phosphate (NADPH). The effect of divicine on purified catalase and on the catalase of intact G6PD-deficient erythrocytes was similar to the previously demonstrated effect on catalase of a known system for generating hydrogen peroxide. This effect of divicine strengthens earlier arguments that divicine is the toxic peroxidative component of fava beans.


Subject(s)
Catalase/physiology , Favism/enzymology , Catalase/antagonists & inhibitors , Child , Child, Preschool , Enzyme Inhibitors/pharmacology , Erythrocytes/drug effects , Erythrocytes/enzymology , Favism/blood , Favism/etiology , Hemolysis , Humans , Hydrogen Peroxide/blood , Male , NADP/blood , Oxidative Stress , Pyrimidinones/pharmacology
11.
J Pediatr ; 127(5): 807-8, 1995 Nov.
Article in English | MEDLINE | ID: mdl-7472841

ABSTRACT

We describe a case of favism in a female newborn infant with glucose-6-phosphate dehydrogenase (G6PD) deficiency whose mother had ingested fava beans 5 days before delivery. At birth there were clinical and hematologic signs of hemolytic anemia, hemoglobinuria, and no blood group immunization. Study of the G6PD activity and 2-deoxy-glucose-6-phosphate utilization rate revealed that the infant and the mother were heterozygous for G6PD deficiency.


Subject(s)
Favism/diagnosis , Prenatal Exposure Delayed Effects , Blood Transfusion , Cesarean Section , Combined Modality Therapy , Favism/blood , Favism/etiology , Favism/therapy , Female , Glucosephosphate Dehydrogenase Deficiency/blood , Heterozygote , Humans , Infant, Newborn , Phototherapy , Pregnancy
12.
Dtsch Med Wochenschr ; 118(25): 932-5, 1993 Jun 25.
Article in German | MEDLINE | ID: mdl-8519217

ABSTRACT

A previously healthy 17-year-old Greek boy suddenly developed jaundice of sclerae and skin. In addition, physical examination revealed a pale appearance. He also reported feeling tired and weak. The haemoglobin level was 9.6 g/dl, lactate dehydrogenase activity 335 U/l, bilirubin concentration 3.2 mg/dl (direct bilirubin 0.7 mg/dl, indirect bilirubin 2.5 mg/dl), haptoglobin concentration 48.8 mg/dl. As haemolytic anaemia was assumed, direct questioning elicited the fact that the patient had, for the first time in his life, eaten 300 g of broad beans (Vicia faba) on each of two days, namely 3 and 2 days before the appearance of jaundice. Absence of glucose-6-phosphate dehydrogenase activity in the red blood corpuscles confirmed the diagnosis of favism. On symptomatic treatment both the enzyme activities and the bilirubin level fell to normal within one week, and the haemoglobin level was 15.7 g/dl after 4 weeks.


Subject(s)
Favism/blood , Acute Disease , Adolescent , Diagnosis, Differential , Favism/complications , Glucosephosphate Dehydrogenase Deficiency/blood , Hepatitis, Viral, Human/diagnosis , Humans , Jaundice/etiology , Male
14.
Cell Calcium ; 13(10): 649-58, 1992 Nov.
Article in English | MEDLINE | ID: mdl-1337501

ABSTRACT

Favism is an acute hemolytic anemia triggered by ingestion of fava beans in genetically susceptible subjects with severe deficiency of glucose-6-phosphate dehydrogenase (G6PD) activity. Erythrocytes from 10 favic patients had constantly and markedly increased calcium levels, as compared with values detected in 4 asymptomatic G6PD-deficient controls. Correspondingly, the calcium permeability of erythrocytes, estimated as the fraction of intracellular calcium exchangeable with externally added 45Ca2+, was invariably enhanced in favism and returned to normal patterns after several months from the acute hemolytic crisis. In favic patients, the levels of erythrocyte calcium ATPase activities showed wide variability, ranging from 2.0-12.9 mumol Pi/ml RBC/h, while control values in asymptomatic G6PD-deficient subjects were 10.62 +/- 2.03 mumol Pi/ml RBC/h. Analysis of the calcium ATPase in situ in erythrocyte membranes from favic patients showed the same molecular mass of 134 kD as observed in the control subjects. Exposure of G6PD-deficient erythrocytes in vitro to autoxidizing divicine, a pyrimidine aglycone strongly implicated in the pathogenesis of favism which leads to late accumulation of intracellular calcium, caused: (i) a marked inactivation of calcium ATPase, without changes in the molecular mass of 134 kD; and (ii) the concomitant loss of spectrin, band 3 and band 4.1, all known substrates of the calcium activated procalpain-calpain proteolytic system. Thus, the increased intraerythrocytic calcium apparently results in the degradation of calcium ATPase observed in some favic patients. It is proposed that both enhanced calcium permeability and a calcium-stimulated degradation of the calcium pump are the mechanisms responsible for the perturbation of erythrocyte calcium homeostasis in favism.


Subject(s)
Calcium-Transporting ATPases , Calcium-Transporting ATPases/blood , Calcium/blood , Erythrocytes/metabolism , Favism/blood , Calcimycin/pharmacology , Calcium-Transporting ATPases/drug effects , Calpain/pharmacology , Glucosephosphate Dehydrogenase/blood , Homeostasis , Humans , Male , Oxidation-Reduction , Pyrimidinones/pharmacology
15.
Int J Exp Pathol ; 73(2): 157-60, 1992 Apr.
Article in English | MEDLINE | ID: mdl-1571275

ABSTRACT

In the present study, plasma lipid concentrations were determined at different times after admission in sera from G6PD-deficient children during haemolytic crisis induced by fava bean ingestion. Reductions in total, LDL and HDL cholesterol were found in association with the maximum of bone marrow hyperplasia. A return towards normal values occurred with regression of the disease. No changes in other lipid parameters were observed. These data suggest that alterations of lipoprotein pattern, other than in experimental animals, are also present in humans with non-malignant proliferative processes. These changes appear to be a consequence of the disease, probably due to an increased utilization of cholesterol by proliferating cells.


Subject(s)
Anemia, Hemolytic/blood , Favism/complications , Glucosephosphate Dehydrogenase Deficiency/blood , Lipoproteins/blood , Anemia, Hemolytic/etiology , Anemia, Hemolytic/pathology , Bone Marrow/pathology , Child , Child, Preschool , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Favism/blood , Female , Humans , Hyperplasia , Male
17.
Pol Tyg Lek ; 45(38-39): 778-81, 1990.
Article in Polish | MEDLINE | ID: mdl-2095545

ABSTRACT

Four cases of fawism are presented. The disease was seen in one male patient, one homozygote and in 3 carriers of G6PD deficit. Diagnostic procedures, course of the haemolytic crisis in these patients, and possibility of prophylaxis in the families with fawism are discussed.


Subject(s)
Anemia, Hemolytic/genetics , Erythrocytes/enzymology , Favism/genetics , Glucosephosphate Dehydrogenase Deficiency/genetics , Glucosephosphate Dehydrogenase/blood , Adult , Anemia, Hemolytic/diagnosis , Anemia, Hemolytic/etiology , Child, Preschool , Diagnosis, Differential , Favism/blood , Favism/diagnosis , Favism/etiology , Female , Glucosephosphate Dehydrogenase Deficiency/complications , Glucosephosphate Dehydrogenase Deficiency/diagnosis , Humans , Male , Poland
19.
Int J Clin Pharmacol Res ; 10(5): 305-10, 1990.
Article in English | MEDLINE | ID: mdl-1981880

ABSTRACT

Tissue hypoperfusion leads to cellular oxidative and peroxidative damage due to biochemical disorders in the oxygen and substrate metabolism. The metabolic turnover of glutathione (GSH) represents one the main cytoprotective systems against the peroxide attack and the depletion or defect in resynthesis of this compound is accompanied by pathological consequences. In the present study the clinical effects of glutathione depletion were investigated in conditions of acute tissue hypoxia due to marked haemolysis in glucose-6-phosphate dehydrogenase deficient patients (favism syndrome). In these subjects a significant marker of the tissue oxidative damage was represented by the uric acid blood levels, presumably linked to xanthine-hypoxanthine altered metabolism. To antagonize the effects of oxyradical pathology, reduced glutathione was administered to a group of patients and the results confirmed the cytoprotective role played by the GSH supplementation. The GSH action was evident on the tissue metabolism and this supports the opinion that reduced glutathione could represent a new and interesting therapeutic approach in marked and acute hypoxic conditions.


Subject(s)
Glucosephosphate Dehydrogenase Deficiency/drug therapy , Glutathione/therapeutic use , Hemolysis/drug effects , Adolescent , Adult , Alanine Transaminase/blood , Aspartate Aminotransferases/blood , Cell Hypoxia/physiology , Double-Blind Method , Favism/blood , Favism/drug therapy , Glucosephosphate Dehydrogenase Deficiency/blood , Glutathione/blood , Humans , Lactates/blood , Lactic Acid , Male , Middle Aged , Oxidation-Reduction , Uric Acid/blood , gamma-Glutamyltransferase/blood
20.
West J Med ; 151(4): 459-60, 1989 Oct.
Article in English | MEDLINE | ID: mdl-2588584
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