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1.
Reprod Fertil Dev ; 32(9): 822-834, 2020 Jun.
Article in English | MEDLINE | ID: mdl-32527373

ABSTRACT

In a feeder-dependent culture system of human pluripotent stem cells (hPSCs), coculture with mouse embryonic fibroblasts may limit the clinical use of hPSCs. The aim of this study was to determine the feasibility of using human Caesarean scar fibroblasts (HSFs) as feeder cells for the culture of hPSCs. HSFs were isolated and characterised and cocultured with hPSCs, and the pluripotency, differentiation ability and karyotypic stability of hPSCs were determined. Inactivated HSFs expressed genes (including inhibin subunit beta A (INHBA), bone morphogenetic protein 4 (BMP4), fibroblast growth factor 2 (FGF2), transforming growth factor-ß1 (TGFB1), collagen alpha-1(I) (COL1A1) and fibronectin-1 (FN1) that have been implicated in the maintenance of hPSC pluripotency. When HSFs were used as feeder cells, the pluripotency and karyotypic stability of hPSC lines did not change after prolonged coculture. Interestingly, exogenous FGF2 could be omitted from the culture medium when HSFs were used as feeder cells for hESCs but not hiPSCs. hESCs cocultured with HSF feeder cells in medium without FGF2 supplementation maintained their pluripotency (as confirmed by the expression of pluripotency markers and genes), differentiated invitro into embryonic germ layers and maintained their normal karyotype. The present study demonstrates that HSFs are a novel feeder cell type for culturing hPSCs and that supplementation of exogenous FGF2 is not necessary for the Chula2.hES line.


Subject(s)
Cesarean Section/adverse effects , Cicatrix/metabolism , Feeder Cells/metabolism , Fibroblasts/metabolism , Paracrine Communication , Pluripotent Stem Cells/metabolism , Cell Differentiation , Cell Line , Cicatrix/etiology , Cicatrix/pathology , Coculture Techniques , Feasibility Studies , Feeder Cells/pathology , Female , Fibroblasts/pathology , Gene Expression Regulation, Developmental , Humans , Karyotype , Phenotype , Pregnancy , Signal Transduction
2.
Probl Radiac Med Radiobiol ; 22: 224-230, 2017 Dec.
Article in English, Ukrainian | MEDLINE | ID: mdl-29286509

ABSTRACT

Under the influence of ionizing radiation on hematopoietic system, the level of its injury is determined not only by the radiosensitivity of hematopoietic stem cells, but also by radiation induced changes in microenvironment func tioning, in particular, mesenchymal stem cells as its components. OBJECTIVE: to define functioning characteristics of mesenchymal stem and progenitor cells of rats' bone marrow under prolonged action of ionizing radiation as a result of 90Sr incorporation. MATERIALS AND METHODS: We applied the model of Wistar rats' internal irradiation with 90Sr radionuclide and per formed the in vitro cultivation of their bone marrow mesenchymal cells. Colony forming efficiency in the in vitro cell culture was determined, as well as the possibility of these cells to form feeder layers and to support rat bone mar row hematopoietic cells in the culture of diffusion chambers in vitro. RESULTS AND CONCLUSIONS: We established that chronic action of incorporated 90Sr radionuclide induced considerable decrease in proliferative activity of mesenchymal stem cells comparing to control, as well as the inhibition of the capability to prolonged support of hematopoietic processes in vitro by their feeder layers.Thus, bone marrow mesenchymal stem cells and their closest progeny - progenitor cells were characterized by rather high radiosensitivity under the influence of ionizing radiation, which was revealed in considerable decline of their functional activity in cell culture in vitro comparing to control indices as a result of irradiation.


Subject(s)
Hematopoietic Stem Cells/radiation effects , Mesenchymal Stem Cells/radiation effects , Radiation Injuries/pathology , Strontium Radioisotopes/administration & dosage , Animals , Bone Marrow/surgery , Cell Proliferation/radiation effects , Colony-Forming Units Assay , Diffusion Chambers, Culture , Feeder Cells/pathology , Feeder Cells/radiation effects , Foreign Bodies/surgery , Mesenchymal Stem Cells/pathology , Primary Cell Culture , Radiation Injuries/etiology , Radiation Tolerance , Radiation, Ionizing , Rats , Rats, Wistar , Strontium Radioisotopes/chemistry
3.
Exp Cell Res ; 322(1): 193-201, 2014 Mar 10.
Article in English | MEDLINE | ID: mdl-24368151

ABSTRACT

In this study, we discovered a subpopulation of 3T3 feeder cells were malignantly transformed by nasopharyngeal carcinoma (NPC) tumor cells during co-culture. The transformed 3T3 cells acquired an accelerated growth rate, displayed loosely attached multilayer growth in vitro and highly tumorigenic in vivo. Most strikingly, instead of forming sarcomas, they developed into carcinoma-like tumors somewhat resembling the original NPC. We further demonstrated the transformation is not a single isolated event, rather a common reproducible, cell contact dispensable phenomena among NPC tumor cells. However, NPC tumor cells alone were not sufficient to confer the transformed characteristics onto normal human cells.


Subject(s)
Cell Transformation, Neoplastic/pathology , Feeder Cells/pathology , Nasopharyngeal Neoplasms/pathology , Primary Cell Culture/methods , Animals , Carcinoma , Coculture Techniques , Female , Fibroblasts/pathology , Fibroblasts/transplantation , Humans , Mice , Mice, Nude , NIH 3T3 Cells , Nasopharyngeal Carcinoma , Tumor Cells, Cultured
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