ABSTRACT
Antibiotic resistance genes (ARGs) have been detected in the atmosphere. Airborne ARGs transmission threatens human health. In the present study, we investigated the release and degradation of airborne ARGs from Escherichia coli bioaerosol through microwave (MW) irradiation. In this study, a new MW absorbing material (Fe3O4@SiC ceramic foam) that contributed to its stronger MW absorption is presented. When the MW input energy density was 7.4 × 103 kJ/m3, the concentration of airborne Escherichia coli decreased by 4.4 log. Different DNA forms were found in the air because MW irradiation ruptured cell membranes. The bound particles provide more protection for bound DNA in the degradation process than free DNA. After the self-degradation of the released airborne free ARGs, some of them would remain and continue to spread in the atmosphere. The released airborne free ARGs cannot be ignored. Total ARGs concentrations decrease rapidly with increased temperature. The inactivation rate constant of ARGs through MW irradiation is higher than that through the Fenton and UV, however, the energy efficiency per order of MW irradiation is lower. Therefore, MW irradiation with Fe3O4@SiC ceramic foam could efficiently degrade the distribution of ARGs in the atmosphere.
Subject(s)
Carbon Compounds, Inorganic/chemistry , Ceramics/chemistry , Drug Resistance, Bacterial/genetics , Escherichia coli/radiation effects , Ferrosoferric Oxide/chemistry , Genes, Bacterial/radiation effects , Silicon Compounds/chemistry , Aerosols/chemistry , Aerosols/radiation effects , Carbon Compounds, Inorganic/radiation effects , Ceramics/radiation effects , DNA, Bacterial/chemistry , DNA, Bacterial/radiation effects , Escherichia coli/chemistry , Escherichia coli/genetics , Ferrosoferric Oxide/radiation effects , Microwaves , Pyrolysis , Silicon Compounds/radiation effects , TemperatureABSTRACT
Magnetic Fe3O4@BiOI@AgI (FBA) spheres were synthesized through a multi-step process. The fabricated photocatalysts were characterized by different techniques. To testify the visible light driven photocatalytic activity of FBA, Rhodamine B and Bisphenol A were chosen as model common and emerging organic contaminants, respectively. While, gram-negative strain Escherichia coli was selected as model waterborne bacteria. The results showed that under visible light irradiation, FBA contained strong photocatalytic degradation capacity towards both RhB and BPA. Moreover, FBA was also found to exhibit excellent disinfection activity towards E. coli. The photocatalytic mechanisms for different pollutants by FBA were determined and found to vary for different pollutants. Specifically, scavenger experiments, degradation intermediates determination, as well as theoretical density functional theory (DFT) analysis showed that RhB and BPA were degraded via photosensitization (dominated by e- and ·O2-) and direct photocatalytic oxidation (contributed by h+, e- and ·O2-), respectively. Whereas, E. coli cells yet were found to be inactivated by the generation of e- and ·O2- rather than by the released Ag+. Since it contained superparamagnetic property, FBA could be easily separated from the reaction suspension after use. Due to the excellent photo stability, FBA exhibited strong photocatalytic activity in the fourth reused recycle. Therefore, FBA could serve as a promising alternative for water purification.
Subject(s)
Bismuth , Disinfection/methods , Ferrosoferric Oxide , Iodides , Light , Silver Compounds , Water Purification/methods , Benzhydryl Compounds/chemistry , Bismuth/chemistry , Catalysis , Decontamination , Escherichia coli/drug effects , Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/radiation effects , Iodides/chemistry , Iodides/radiation effects , Phenols/chemistry , Rhodamines/chemistry , Silver Compounds/chemistry , Silver Compounds/radiation effects , Water Pollutants, Chemical/chemistryABSTRACT
Dihydroartemisinin (DHA) has been investigated in cancer therapy for its reactive oxygen species (ROS) based cytotoxicity originated from interacting with ferrous ions that may reduce or eliminate the multidrug resistance commonly associated with conventional chemotherapy agents. However, synchronously delivery of hydrophobic DHA and Fe (â ¢) ions into tumor cells remains a major challenge. In this work, we develop novel Fe3O4@C@MIL-100(Fe) (FCM) nanoparticles for synchronously delivery of DHA and Fe (â ¢) for cancer therapy. The MOFs structure based on Fe (â ¢) carboxylate materials MIL-100 (Fe) holds great potential for storage/delivery of hydrophobic drug DHA. As a unique nanoplatform, the hybrid inorganic-organic drug delivery vehicles show pH-responsive biodegradation and synchronous releasing of DHA and Fe (â ¢) upon reaching tumor sites. The intracellular Fe (â ¢) will be reduced further to ferrous ion and interact with DHA to increase its cytotoxicity. The potential of this alternative anti-tumor modality is demonstrated in vivo due to an increased intracellular accumulation of DHA in tumor and activated mechanism via co-release of DHA and Fe (â ¢), especially under the guidance of an external applied magnetic field.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Drug Delivery Systems/methods , Ferrosoferric Oxide/chemistry , Nanocapsules/chemistry , Neoplasms, Experimental/drug therapy , Absorption, Physicochemical , Animals , Antineoplastic Agents/administration & dosage , Antineoplastic Agents/chemistry , Artemisinins/administration & dosage , Artemisinins/chemistry , Drug Synergism , Female , Ferrosoferric Oxide/radiation effects , HeLa Cells , Humans , Hydrogen-Ion Concentration , Ions/administration & dosage , Ions/chemistry , Iron/administration & dosage , Iron/chemistry , Magnetic Fields , Mice , Mice, Inbred BALB C , Mice, Nude , Nanocapsules/radiation effects , Treatment OutcomeABSTRACT
The first one-step synthesis of dumbbell-like gold-iron oxide nanoparticles has been reported here. Surface functionalization with a biocompatible chitosan matrix allowed us to obtain a novel targetable diagnostic and therapeutic tool.
Subject(s)
Chitosan/chemistry , Ferrosoferric Oxide/chemistry , Gold/chemistry , Metal Nanoparticles/chemistry , Chitosan/radiation effects , Energy Transfer , Ferrosoferric Oxide/radiation effects , Gold/radiation effects , Light , Metal Nanoparticles/radiation effects , Molecular Structure , Particle Size , Photoacoustic Techniques , Surface Properties , Theranostic Nanomedicine/instrumentationABSTRACT
Fe2O3 supported on activated carbon fiber (Fe2O3/ACF) was prepared via an impregnation method and characterized by X-ray diffraction, scanning electron microscopy and BET analysis. The results indicated that Fe2O3 with small particle size was highly dispersed on the surface of the ACF and the introduction of Fe2O3 did not change the ACF pore structure. Fe2O3/ACF exhibited a higher Fenton efficiency for the degradation of acid red B (ARB), especially under simulated solar irradiation. Complete decoloration of the ARB solution and 43% removal of TOC could be achieved within 200 min under optimal conditions. It was verified that more ËOH radicals were generated in the photo-assisted Fenton process and involved as active species in ARB degradation. FTIR analysis indicated that the degradation of ARB was initiated through the cleavage of -N=N-, followed by hydroxylation and opening of phenyl rings to form aliphatic acids, and further oxidation of aliphatic acids would produce CO2 and H2O. Moreover, Fe2O3/ACF maintained its activity after being reused 4 times and the release of iron from the catalyst was found to be insignificant during the Fenton and photo-Fenton processes, indicating that Fe2O3/ACF had good long-term stability.
Subject(s)
Azo Compounds/chemistry , Carbon/radiation effects , Ferrosoferric Oxide/radiation effects , Naphthalenesulfonates/chemistry , Water Pollutants, Chemical/chemistry , Carbon/chemistry , Carbon Fiber , Ferrosoferric Oxide/chemistry , Hydrogen Peroxide/chemistry , Light , Waste Disposal, Fluid/methodsABSTRACT
Conservation of energy by Fe(III)-reducing species such as Shewanella oneidensis could potentially control the redox potential of environments relevant to the geological disposal of radioactive waste and radionuclide contaminated land. Such environments will be exposed to ionizing radiation so characterization of radiation alteration to the mineralogy and the resultant impact upon microbial respiration of iron is essential. Radiation induced changes to the iron mineralogy may impact upon microbial respiration and, subsequently, influence the oxidation state of redox-sensitive radionuclides. In the present work, Mössbauer spectroscopy and electron microscopy indicate that irradiation (1 MGy gamma) of 2-line ferrihydrite can lead to conversion to a more crystalline phase, one similar to akaganeite. The room temperature Mössbauer spectrum of irradiated hematite shows the emergence of a paramagnetic Fe(III) phase. Spectrophotometric determination of Fe(II) reveals a radiation-induced increase in the rate and extent of ferrihydrite and hematite reduction by S. oneidensis in the presence of an electron shuttle (riboflavin). Characterization of bioreduced solids via XRD indicate that this additional Fe(II) is incorporated into siderite and ferrous hydroxy carbonate, along with magnetite, in ferrihydrite systems, and siderite in hematite systems. This study suggests that mineralogical changes to ferrihydrite and hematite induced by radiation may lead to an increase in bioavailability of Fe(III) for respiration by Fe(III)-reducing bacteria.
Subject(s)
Ferric Compounds/chemistry , Ferric Compounds/radiation effects , Gamma Rays , Shewanella/growth & development , Biodegradation, Environmental , Biological Availability , Carbonates/chemistry , Carbonates/radiation effects , Electrons , Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/radiation effects , Microscopy, Electron, Transmission , Oxidation-Reduction , Shewanella/metabolism , Shewanella/radiation effects , Spectroscopy, MossbauerABSTRACT
Extremely simple procedures for the preparation of magnetic chitosan enzyme carriers have been developed and used for the immobilization of lipase and ß-galactosidase as model enzymes. In the first case commercially available magnetite microparticles were entrapped in chitosan gel, while in the second case magnetic iron oxides microparticles were synthesized in chitosan matrix from ferrous sulfate using microwave irradiation. Immobilized enzymes showed long-term stability without leaching of enzyme from the support and enabled their repeated use without significant loss of activity.
Subject(s)
Chitosan/chemistry , Enzymes, Immobilized/chemistry , Ferrosoferric Oxide/chemistry , Microspheres , Chitosan/radiation effects , Ferrosoferric Oxide/radiation effects , Lipase/chemistry , Microwaves , beta-Galactosidase/chemistryABSTRACT
This study is to optimize the multi-quality performance of magnetic catalyst/ozone process by combining a technique for order performance by similarity to ideal solution (TOPSIS) with the Taguchi method, which simultaneously has the best decomposition rate constant of benzoic acid and removal rate constant of total organic carbon (TOC). The optimal experimental parameters were pH of 7, initial concentration of 75 ppm and catalyst loading of 0.05 g/L. More than 93% of the magnetic catalyst was easily separated and redispersed for reuse by the magnetic force due to the paramagnetic behaviours of the prepared SiO2/Fe3O4. It is believed that through the joint efforts improvement, design and manufacturing, new separation and recycling technologies will be available and more easily recyclable magnetic catalysts will be developed in the future.
Subject(s)
Benzoic Acid/chemistry , Benzoic Acid/radiation effects , Ferrosoferric Oxide/chemistry , Ozone/chemistry , Water Pollutants, Chemical/chemistry , Water Pollutants, Chemical/isolation & purification , Water Purification/methods , Benzoic Acid/isolation & purification , Catalysis , Ferrosoferric Oxide/radiation effects , Magnetic Fields , Ozone/radiation effects , Recycling , Water/chemistry , Water Pollutants, Chemical/radiation effectsABSTRACT
Two-dimensional plate-like Fe(3)O(4) nanocrystals were synthesized by a facile method using ultrasonic irradiation in aqueous solution at low temperature without protection from oxygen. The crystals were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier Transform infrared spectroscopy. The products subjected to ultrasound showed a two-dimensional morphology. The results obtained indicate that the morphologies of the magnetite crystals depend more on the ultrasonic irradiation than on the growth temperature. The thickness and width of the crystals increased with increasing temperature of the reaction medium. In addition, the magnetic hysteresis loop of the magnetite nanoplates was obtained at room temperature.
Subject(s)
Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/radiation effects , Nanostructures/chemistry , Nanostructures/radiation effects , Nanotechnology/methods , Sonication/methods , Cold Temperature , Nanostructures/ultrastructure , Particle Size , Radiation DosageABSTRACT
Detailed observations of the single mode H-field heated magnetite was conducted. The heated magnetite contains a shiny area, which exists in the width of 5 mm and thickness in 1 mm. The observation of the area reveals wavy surface patterns. The microstructures of the shiny area consists of an undefined phase and a magnetite phase which fills the space among the undefined phases. The XRD pattern from the shiny area is different from that of the random magnetite crystals, which is caused by the magnetite grains having almost same orientation in large area. There are banded areas surrounding the undefined phase of several microns in thickness, in which the existence of nano-sized crystals is indicated by means of EBSD and TEM.
Subject(s)
Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/radiation effects , Heating/instrumentation , Microwaves , Equipment Design , Equipment Failure Analysis , Ferrosoferric Oxide/analysis , Materials Testing , Particle Size , Radiation Dosage , Surface Properties/radiation effectsABSTRACT
The field dependence of the optical transmission of tartrate-coated and polyaspartate-coated magnetite-based aqueous colloids was studied. The colloidal stock samples were diluted to prepare a series of samples containing different particle volume fractions ranging from 0.17% up to 1.52% and measured at distinct times after preparation (1, 30, 120, 240, and 1460 days). We show that the magneto-transmissivity behavior is mainly described by the rotation of linear chains, at the low-field range, whereas the analysis of the data provided the measurement of the average chain length. Results also reveal that the optical transmissivity has a minimum at a particular critical field, whose origin is related to the onset of columns of chains built from isolated particle chains, i.e., due to a columnar phase transition. We found the critical field reducing as the particle volume fraction increases and as the sample's aging time increases. To investigate the origin of this phenomenon we used phase condensation models and Mie's theory applied to a chain of spheres and to an infinite cylinder. Possible implications for magnetophotonic colloidal-based devices and biomedical applications were discussed.
Subject(s)
Biocompatible Materials/chemistry , Biocompatible Materials/radiation effects , Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/radiation effects , Models, Chemical , Computer Simulation , Electric Impedance , Electromagnetic Fields , Magnetics , Phase Transition/radiation effectsABSTRACT
Nanoscale assemblies that can be activated and controlled through external stimuli represent a next stage in multifunctional therapeutics. We report the formation, characterization, and release properties of bilayer-decorated magnetoliposomes (dMLs) that were prepared by embedding small hydrophobic SPIO nanoparticles at different lipid molecule to nanoparticle ratios within dipalmitoylphosphatidylcholine (DPPC) bilayers. The dML structure was examined by cryogenic transmission electron microscopy and differential scanning calorimetry, and release was examined by carboxyfluorescein leakage. Nanoparticle heating using alternating current electromagnetic fields (EMFs) operating at radio frequencies provided selective release of the encapsulated molecule at low nanoparticle concentrations and under physiologically acceptable EMF conditions. Without radio frequency heating, spontaneous leakage from the dMLs decreased with increasing nanoparticle loading, consistent with greater bilayer stability and a decrease in the effective dML surface area due to aggregation. With radio frequency heating, the initial rate and extent of leakage increased significantly as a function of nanoparticle loading and electromagnetic field strength. The mechanism of release is attributed to a combination of bilayer permeabilization and partial dML rupture.
Subject(s)
Crystallization/methods , Delayed-Action Preparations/chemistry , Ferrosoferric Oxide/chemistry , Lipid Bilayers/chemistry , Liposomes/chemistry , Electromagnetic Fields , Ferrosoferric Oxide/radiation effects , Hot Temperature , Lipid Bilayers/radiation effects , Liposomes/radiation effects , Macromolecular Substances/chemistry , Materials Testing , Molecular Conformation , Nanotechnology/methods , Particle Size , Surface PropertiesABSTRACT
Magnetoresponsive smart capsules formed with polyelectrolytes, lipid bilayers and magnetic nanoparticles were fabricated by a colloid-templating technique. Melamine-formaldehyde core particles with polyelectrolyte multilayer shell were prepared by layer-by-layer assembly. Magnetite (Fe(3)O(4)) nanoparticles were selectively deposited on the capsular surface by aqueous solution deposition using Pd catalysts. Hollow capsules were obtained by the removal of the melamine formaldehyde core particles. Vibrating sample magnetometer (VSM) measurement of the capsules revealed the ferromagnetic behavior of deposited Fe(3)O(4) nanoparticles. Alternating magnetic field irradiation generates heat in the capsular dispersion. Additional lipid bilayer coating was carried out on the obtained hollow capsules. Dye molecules were loaded by exploiting the temperature-dependence of the lipid membrane permeability. An encapsulated dye was released on-demand by irradiation with an alternating magnetic field, due to a phase transition in the lipid membrane, induced by heating of the magnetic nanoparticles. The magnetically induced release is attributed to the phase transition of the lipid membrane, caused by heat of Fe(3)O(4) nanoparticles under magnetic stimuli, and not to rupture of the capsules.
Subject(s)
Capsules/chemistry , Electrolytes/chemistry , Ferrosoferric Oxide/chemistry , Lipid Bilayers/chemistry , Nanotechnology/methods , Capsules/radiation effects , Electrolytes/radiation effects , Electromagnetic Fields , Ferrosoferric Oxide/radiation effects , Lipid Bilayers/radiation effects , Materials Testing , Particle SizeABSTRACT
PURPOSE: Magnetic particle imaging (MPI) is a new quantitative imaging technique capable of determining the spatial distribution of superparamagnetic nanoparticles at high temporal and spatial resolution. For reconstructing this spatial distribution, the particle dynamics and the scanner properties have to be known. To date, they are obtained in a tedious calibration procedure by measuring the magnetization response of a small delta sample shifted through the measuring field. Recently, first reconstruction results using a 1D model-based system function were published, showing comparable image quality as obtained with a measured system function. In this work, first 2D model-based reconstruction results of measured MPI data are presented. METHODS: To simulate the system function, various parameters have to be modeled, namely, the magnetic field, the particle magnetization, the voltage induced in the receive coils, and the transfer function of the receive chain. To study the accuracy of the model-based approach, 2D MPI data are measured and reconstructed with modeled and measured system functions. RESULTS: It is found that the model-based system function is sufficiently accurate to allow for reconstructing experimental data. The resulting image quality is close to that obtained with a measurement-based reconstruction. CONCLUSIONS: The model-based system function approach addresses a major drawback of the measurement-based procedure, namely, the long acquisition time. In this work, the acquisition of the measurement-based system function took 45 min, while the model-based system function was obtained in only 15 s. For 3D data, where the acquisition of the measurement-based system function takes more than 6 h, the need for an efficient system function generation is even more obvious.
Subject(s)
Contrast Media/chemistry , Ferrosoferric Oxide/chemistry , Image Interpretation, Computer-Assisted/methods , Magnetic Resonance Imaging/methods , Models, Chemical , Computer Simulation , Contrast Media/radiation effects , Electromagnetic Fields , Ferrosoferric Oxide/radiation effects , Magnetics/methodsABSTRACT
It is now well established that animals use the Earth's magnetic field to perform long-distance migration and other navigational tasks. However, the transduction mechanisms that allow the conversion of magnetic field variations into an electric signal by specialized sensory cells remain largely unknown. Among the species that have been shown to sense Earth-strength magnetic fields, birds have been a model of choice since behavioural tests show that their direction-finding abilities are strongly influenced by magnetic fields. Magnetite, a ferromagnetic mineral, has been found in a wide range of organisms, from bacteria to vertebrates. In birds, both superparamagnetic (SPM) and single-domain magnetite have been found to be associated with the trigeminal nerve. Electrophysiological recordings from cells in the trigeminal ganglion have shown an increase in action potential firing in response to magnetic field changes. More recently, histological evidence has demonstrated the presence of SPM magnetite in the subcutis of the pigeon's upper beak. The aims of the present review are to review the evidence for a magnetite-based mechanism in birds and to introduce physiological concepts in order to refine the proposed models.
Subject(s)
Animal Migration/physiology , Animal Migration/radiation effects , Birds/physiology , Ferrosoferric Oxide/metabolism , Orientation/physiology , Orientation/radiation effects , Perception/physiology , Perception/radiation effects , Animals , Electromagnetic Fields , Ferrosoferric Oxide/radiation effects , Humans , LightABSTRACT
Magnetite nanoparticles were coated with silica through the hydrolysis and condensation of tetraethyl orthosilicate (TEOS) under ultrasonic irradiation. The ultrasonic irradiation was used to prevent the agglomeration of the magnetite particles and accelerate the hydrolysis and condensation of TEOS. TEM, DLS, XRF, VSM, TG and sedimentation test were used to characterize the silica-coated magnetite particles. The dispersibility of silica-coated magnetite particles in aqueous solution was improved significantly and the agglomerate particle size was decreased to 110 nm. It was found that the agglomerate particle size of silica-coated magnetite particles was mainly decided by the coating temperature and the pH value in the silica-coating process. The weight ratio of silica in silica-coated magnetite particles was mainly decided by the pH value in the silica-coating process. The dispersibility of silica-coated magnetite particles was mainly decided by the agglomerate particle size of the suspension. The oxidation of magnetite particles in air was limited through the coated silica. The magnetism of silica-coated magnetite particles decreased slightly after silica-coating.
Subject(s)
Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/radiation effects , Nanoparticles/chemistry , Nanoparticles/radiation effects , Silicon Dioxide/chemistry , Silicon Dioxide/radiation effects , Sonication/methods , Adsorption/radiation effects , Nanoparticles/ultrastructure , Particle Size , Radiation DosageSubject(s)
Magnetics , Nanotubes/chemistry , Pharmaceutical Preparations/chemistry , Titanium/chemistry , Catalysis , Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/radiation effects , Nanoparticles/chemistry , Nanoparticles/radiation effects , Nanotubes/radiation effects , Particle Size , Pharmaceutical Preparations/radiation effects , Photochemistry , Surface Properties , Titanium/radiation effects , Ultraviolet RaysABSTRACT
Ordered mesoporous materials like SBA-15 have a network of channels and pores with well-defined size in the nanoscale range. This particular silica matrix pore architecture makes them suitable for hosting a broad variety of compounds in very promising materials in a range of applications, including drug release magnetic carriers. In this work, magnetic nanoparticles embedded into mesoporous silica were prepared in two steps: first, magnetite was synthesized by oxidation-precipitation method, and next, the magnetic nanoparticles were coated with mesoporous silica by using nonionic block copolymer surfactants as structure-directing agents. The materials were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), N(2) adsorption, and scanning electron microscopy (SEM). The influence of magnetic nanoparticles on drug release kinetics was studied with cisplatin, carboplatin, and atenolol under in vitro conditions in the absence and in the presence of an external magnetic field (0.25 T) by using NdFeB permanent magnet. The constant external magnetic field did not affect drug release significantly. The low-frequency alternating magnetic field had a large influence on the cisplatin release profile.
Subject(s)
Delayed-Action Preparations/chemistry , Ferrosoferric Oxide/chemistry , Pharmaceutical Preparations/administration & dosage , Pharmaceutical Preparations/chemistry , Silicon Dioxide/chemistry , Absorption , Delayed-Action Preparations/radiation effects , Diffusion , Electromagnetic Fields , Ferrosoferric Oxide/radiation effects , Materials Testing , Pharmaceutical Preparations/radiation effects , Porosity , Radiation Dosage , Silicon Dioxide/radiation effectsABSTRACT
Off-resonance imaging (ORI) techniques are being increasingly used to image iron oxide imaging agents such as monocrystalline iron oxide nanoparticles (MION). However, the diagnostic accuracy, linearity, and field dependence of ORI have not been fully characterized. In this study, the sensitivity, specificity, and linearity of ORI were thus examined as a function of both MION concentration and magnetic field strength (4.7 and 14 T). MION phantoms with and without an air interface as well as MION uptake in a mouse model of healing myocardial infarction were imaged. MION-induced resonance shifts were shown to increase linearly with MION concentration. In contrast, the ORI signal/sensitivity was highly non-linear, initially increasing with MION concentration until T2 became comparable to the TE and decreasing thereafter. The specificity of ORI to distinguish MION-induced resonance shifts from on-resonance water was found to decrease with increasing field because of the increased on-resonance water linewidths (15 Hz at 4.7 T versus 45 Hz at 14 T). Large resonance shifts ( approximately 300 Hz) were observed at air interfaces at 4.7 T, both in vitro and in vivo, and led to poor ORI specificity for MION concentrations less than 150 microg Fe/mL. The in vivo ORI sensitivity was sufficient to detect the accumulation of MION in macrophages infiltrating healing myocardial infarcts, but the specificity was limited by non-specific areas of positive contrast at the air/tissue interfaces of the thoracic wall and the descending aorta. Improved specificity and linearity can, however, be expected at lower fields where decreased on-resonance water linewidths, reduced air-induced resonance shifts, and longer T2 relaxation times are observed. The optimal performance of ORI will thus likely be seen at low fields, with moderate MION concentrations and with sequences containing very short TEs.
Subject(s)
Energy Transfer/radiation effects , Magnetics , Spin Labels , Animals , Diagnostic Imaging/methods , Disease Models, Animal , Ferrosoferric Oxide/chemistry , Ferrosoferric Oxide/pharmacokinetics , Ferrosoferric Oxide/radiation effects , Heart/physiopathology , Macrophages/pathology , Magnetic Resonance Spectroscopy , Mice , Myocardial Infarction/physiopathology , Nanoparticles/chemistry , Nanoparticles/radiation effects , Phantoms, Imaging , Reference Values , Reproducibility of Results , Sensitivity and Specificity , Vibration , Water/chemistryABSTRACT
Technologies for fabricating functional tissue architectures by patterning cells precisely are highly desirable for tissue engineering. Although several cell patterning methods such as microcontact printing and lithography have been developed, these methods require specialized surfaces to be used as substrates, the fabrication of which is time consuming. In the present study, we demonstrated a simple and rapid cell patterning technique, using magnetite nanoparticles and magnetic force, which enables us to allocate cells on arbitrary surfaces. Magnetite cationic liposomes (MCLs) developed in our previous study were used to magnetically label the target cells. When steel plates placed on a magnet were positioned under a cell culture surface, the magnetically labeled cells lined on the surface where the steel plate was positioned. Patterned lines of single cells were achieved by adjusting the number of cells seeded, and complex cell patterns (curved, parallel, or crossing patterns) were successfully fabricated. Since cell patterning using magnetic force may not limit the property of culture surfaces, human umbilical vein endothelial cells (HUVECs) were patterned on Matrigel, thereby forming patterned capillaries. These results suggest that the novel cell patterning methodology, which uses MCLs, is a promising approach for tissue engineering and studying cell-cell interactions in vitro.