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1.
Comput Math Methods Med ; 2022: 3331119, 2022.
Article in English | MEDLINE | ID: mdl-35720042

ABSTRACT

Objective: To explore the effects of fibroblast growth factor 3 (FGF3) on the proliferation, cell cycle, and apoptosis of the tongue squamous cell carcinoma SCC-9 cell line (SCC-9). Methods: We measured the proliferation of SCC-9 cells in a control group, an FGF3 intervention group, and a fibroblast growth factor (FGFR) inhibitor intervention group in cholecystokinin octapeptide (CCK-8) experiments. We studied effects of FGF3 on the cell cycle and apoptosis of tongue cancer cells using flow cytometry. We further explored the IRS1/PI3K/AKT signaling pathway by measuring BCL-2 and Bcl-2 Associated X-protein (BAX) mRNA and protein levels with RT-PCR and western blot, respectively. Results: Results from the CCK-8 experiment showed that survival rates of cells in the control group, FGF3 intervention group, and FGFR inhibitor intervention group were 100.000% ± 4.026%, 136.330% ± 9.779%, and 83.199% ± 4.954%, respectively; survival rates of SCC-9 cells in all three groups were statistically significant (P < 0.05). Compared with that in the control group, the ratio of cells in G0/G1 phase in the FGFR inhibitor intervention group was higher (P < 0.05) and that in G2/M phase was lower, while the FGF3 intervention group showed opposite results (P < 0.05). The apoptosis rate of tongue cancer cells differed significantly between the FGFR inhibitor intervention and the control groups (P < 0.05). The mRNA and protein expression levels of IRS1, PI3K, and BCL-2 were all increased in the FGF3 intervention group (P < 0.05), while BAX mRNA and protein expression levels were decreased (P < 0.05). The mRNA expression levels of protein kinase B (AKT) showed no differences between groups. The p-AKT protein was overexpressed, while the total amount of AKT protein remained stable (P < 0.05). Conclusion: FGF3 contributes to the proliferation of SCC-9 cells by increasing the proportion of cells in G2/M phase. Therefore, appropriately timed inhibition of FGF3 can potentially promote tumor apoptosis through the IRS1/PI3K/AKT signaling pathway. Our results demonstrate the role of FGF3 in the tumor microenvironment in tongue squamous cell carcinoma SCC-9 cells and suggest new therapeutic targets.


Subject(s)
Carcinoma, Squamous Cell , Tongue Neoplasms , Apoptosis/genetics , Carcinoma, Squamous Cell/metabolism , Cell Line, Tumor , Cell Proliferation/genetics , Fibroblast Growth Factor 3/pharmacology , Humans , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/pharmacology , Proto-Oncogene Proteins c-bcl-2/therapeutic use , RNA, Messenger/genetics , Sincalide/pharmacology , Sincalide/therapeutic use , Tongue , Tongue Neoplasms/drug therapy , Tongue Neoplasms/genetics , Tongue Neoplasms/pathology , Tumor Microenvironment , bcl-2-Associated X Protein/pharmacology
2.
Mol Ther ; 27(6): 1101-1113, 2019 06 05.
Article in English | MEDLINE | ID: mdl-31005598

ABSTRACT

Most cases of sensorineural deafness are caused by degeneration of hair cells. Although stem/progenitor cell therapy is becoming a promising treatment strategy in a variety of organ systems, cell engraftment in the adult mammalian cochlea has not yet been demonstrated. In this study, we generated human otic progenitor cells (hOPCs) from induced pluripotent stem cells (iPSCs) in vitro and identified these cells by the expression of known otic markers. We showed successful cell transplantation of iPSC-derived-hOPCs in an in vivo adult guinea pig model of ototoxicity. The delivered hOPCs migrated throughout the cochlea, engrafted in non-sensory regions, and survived up to 4 weeks post-transplantation. Some of the engrafted hOPCs responded to environmental cues within the cochlear sensory epithelium and displayed molecular features of early sensory differentiation. We confirmed these results with hair cell progenitors derived from Atoh1-GFP mice as donor cells. These mouse otic progenitors transplanted using the same in vivo delivery system migrated into damaged cochlear sensory epithelium and adopted a partial sensory cell fate. This is the first report of the survival and differentiation of hOPCs in ototoxic-injured mature cochlear epithelium, and it should stimulate further research into cell-based therapies for treatment of deafness.


Subject(s)
Cell Enlargement , Hair Cells, Auditory/drug effects , Hearing Loss/surgery , Induced Pluripotent Stem Cells/metabolism , Induced Pluripotent Stem Cells/transplantation , Ototoxicity/surgery , Stem Cell Transplantation/methods , Amikacin/adverse effects , Amikacin/pharmacology , Animals , Auditory Threshold/drug effects , Cell Differentiation/drug effects , Cell Movement/drug effects , Cyclosporine/pharmacology , Disease Models, Animal , Fibroblast Growth Factor 10/pharmacology , Fibroblast Growth Factor 3/pharmacology , Guinea Pigs , Hair Cells, Auditory/immunology , Hair Cells, Auditory/metabolism , Hearing Loss/chemically induced , Humans , Immunosuppressive Agents/pharmacology , Induced Pluripotent Stem Cells/immunology , Living Donors
3.
Cancer Sci ; 106(10): 1278-87, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26183471

ABSTRACT

Cancer-associated fibroblasts (CAFs), as the activated fibroblasts in the tumor stroma, are important modifiers of tumour progression. In the present study, we observed that azoxymethane and dextran sodium sulfate treatments induced increasingly severe colorectal mucosal inflammation and the intratumoural accumulation of CAFs. Fibroblast growth factor (FGF)-1 and FGF-3 were detected in infiltrating cells, and FGFR4, the specific receptor for FGF-1 and FGF-3, was detected in colon cancer tissues. The phosphorylation of FGFR4 enhanced the production of metalloproteinase (MMP)-7 and mitogen-activated protein kinase kinase (Mek)/extracellular signal-regulated kinase (Erk), which was accompanied by excessive vessel generation and cell proliferation. Moreover, we separated CAFs, pericarcinoma fibroblasts (PFs), and normal fibroblasts (NFs) from human colon tissue specimens to characterize the function of CAFs. We observed that CAFs secrete more FGF-1/-3 than NFs and PFs and promote cancer cell growth and angiogenesis through the activation of FGFR4, which is followed by the activation of Mek/Erk and the modulation of MMP-7 expression. The administration of FGF-1/-3-neutralizing antibodies or the treatment of cells with FGFR4 siRNA or the FGFR4 inhibitor PD173074 markedly suppressed colon cancer cell proliferation and neovascularization. These observations suggest a crucial role for CAFs and FGF signaling in the initiation and progression of colorectal cancer. The inhibition of the FGF signaling pathway may be a useful strategy for the treatment of colon cancer.


Subject(s)
Colitis, Ulcerative/pathology , Colonic Neoplasms/pathology , Extracellular Signal-Regulated MAP Kinases/metabolism , Fibroblast Growth Factor 1/metabolism , Fibroblast Growth Factor 3/metabolism , Matrix Metalloproteinase 7/metabolism , Receptor, Fibroblast Growth Factor, Type 4/metabolism , Animals , Azoxymethane , Cell Line, Tumor , Colitis, Ulcerative/metabolism , Colonic Neoplasms/genetics , Dextran Sulfate , Disease Models, Animal , Disease Progression , Fibroblast Growth Factor 3/pharmacology , Fibroblasts/metabolism , HCT116 Cells , HT29 Cells , Human Umbilical Vein Endothelial Cells , Humans , MAP Kinase Signaling System/genetics , Mice , Phosphorylation , Pyrimidines/pharmacology , RNA Interference , RNA, Small Interfering , Receptor Protein-Tyrosine Kinases/antagonists & inhibitors , Receptor, Fibroblast Growth Factor, Type 4/antagonists & inhibitors , Receptor, Fibroblast Growth Factor, Type 4/genetics , Receptors, Fibroblast Growth Factor/antagonists & inhibitors
4.
Development ; 140(21): 4375-85, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24067353

ABSTRACT

Odontoblasts and osteoblasts develop from multipotent craniofacial neural crest cells during tooth and jawbone development, but the mechanisms that specify and sustain their respective fates remain largely unknown. In this study we used early mouse molar and incisor tooth germs that possess distinct tooth-forming capability after dissociation and reaggregation in vitro to investigate the mechanism that sustains odontogenic fate of dental mesenchyme during tooth development. We found that after dissociation and reaggregation, incisor, but not molar, mesenchyme exhibits a strong osteogenic potency associated with robustly elevated ß-catenin signaling activity in a cell-autonomous manner, leading to failed tooth formation in the reaggregates. Application of FGF3 to incisor reaggregates inhibits ß-catenin signaling activity and rescues tooth formation. The lack of FGF retention on the cell surface of incisor mesenchyme appears to account for the differential osteogenic potency between incisor and molar, which can be further attributed to the differential expression of syndecan 1 and NDST genes. We further demonstrate that FGF signaling inhibits intracellular ß-catenin signaling by activating the PI3K/Akt pathway to regulate the subcellular localization of active GSK3ß in dental mesenchymal cells. Our results reveal a novel function for FGF signaling in ensuring the proper fate of dental mesenchyme by regulating ß-catenin signaling activity during tooth development.


Subject(s)
Cell Differentiation/physiology , Fibroblast Growth Factor 3/metabolism , Mesoderm/embryology , Odontogenesis/physiology , Signal Transduction/physiology , Tooth/embryology , Animals , Fibroblast Growth Factor 3/pharmacology , Galactosides , Immunoblotting , Immunohistochemistry , In Situ Hybridization , Indoles , Mesoderm/cytology , Mice , Microspheres , Neural Crest/cytology , Signal Transduction/drug effects , Signal Transduction/genetics , beta Catenin/metabolism
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