Subject(s)
Anti-Bacterial Agents/chemistry , Flexibacter/chemistry , Macrolides/chemistry , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Gram-Positive Bacteria/drug effects , Isomerism , Macrolides/isolation & purification , Macrolides/pharmacology , Magnetic Resonance Spectroscopy , Molecular ConformationABSTRACT
The taxonomic relationship between [Flexibacter] aggregans IAM 14894(T) and Flexithrix dorotheae NBRC 15987(T) was investigated by means of DNA-DNA hybridization and phenotypic characteristics. On the basis of these results, it is proposed that Flexibacter aggregans (Lewin 1969) Leadbetter 1974 be considered a later heterotypic synonym of Flexithrix dorotheae Lewin 1970. Emended descriptions of the species Flexithrix dorotheae and the genus Flexithrix are also given.
Subject(s)
Flexibacter/classification , Base Composition , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Flexibacter/chemistry , Flexibacter/genetics , Genes, rRNA , Molecular Sequence Data , Nucleic Acid Hybridization , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sequence Homology, Nucleic AcidABSTRACT
A Gram-negative bacterium, designated strain LMG 22510T, was isolated from water of a pharmaceutical company steam generator. The cells had a ring-like and horseshoe-shaped morphology and possessed gliding motility. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain was a member of the Flexibacter group within the phylum 'Bacteroidetes'; its nearest neighbour was Spirosoma linguale (88.8 % sequence similarity). DNA base content, fatty acid composition and biochemical characteristics were determined. Genotypic and phenotypic data indicated that strain LMG 22510T could not be assigned to any recognized genus; therefore, a novel genus and species is proposed, Larkinella insperata gen. nov., sp. nov., with LMG 22510T (= NCIMB 14103T) as the type strain.
