Impact of galactic cosmic ray simulation on nutritional content of foods.

Foods packaged for future deep-space exploration missions may be prepositioned ahead of astronaut arrival and will be exposed to galactic cosmic rays (GCRs) and solar radiation in deep space at higher levels and different spectrums than those found in low-Earth orbit (LEO). In this study, we have evaluated the impact of a GCR simulation (approximately 0.5 and 5 Gy doses) at the NASA Space Radiation Laboratory (NSRL) on two retort thermostabilized food products that are good sources of radiation labile nutrients (thiamin, vitamin E, or unsaturated fats). No trends or nutritional differences were found between the radiation-treated samples and the control immediately after treatment or one-year after treatment. Small changes in a few nutrients were measured following one-year of storage. Further studies may be needed to confirm these results, as the foods in this study were heterogeneous, and this may have masked meaningful changes due to pouch-to-pouch variations.

Ionizing Radiation Technologies to Increase the Extraction of Bioactive Compounds from Agro-Industrial Residues: A Review.

Due to the growing demand in society for healthier foods, scientific communities are searching and developing new ingredients. In this context, agro-industrial residues, which can have a negative impact on the environment, represent a natural source for bioactive compounds and their recovery can contribute to economic and environmental sustainability. Ionizing radiation is a clean and eco-friendly technology that can be used to improve the extraction of bioactive compounds. The aim of this review, after presenting general aspects about bioactive compounds in agro-industrial residues and radiation technologies, is to focus on the effects of ionizing radiation on the extraction of bioactive compounds from these residues and related bioactive properties. Irradiated residues were demonstrated to have enhanced bioactive characteristics that turn the prepared extracts suitable for applications in food industry, resulting in high-added-value products as well as reducing adverse impacts on the environment.

Effect of Microwave Heating on the Acrylamide Formation in Foods.

Acrylamide (AA) is a neurotoxic and carcinogenic substance that has recently been discovered in food. One of the factors affecting its formation is the heat treatment method. This review discusses the microwave heating as one of the methods of thermal food processing and the influence of microwave radiation on the acrylamide formation in food. In addition, conventional and microwave heating were compared, especially the way they affect the AA formation in food. Available studies demonstrate differences in the mechanisms of microwave and conventional heating. These differences may be beneficial or detrimental depending on different processes. The published studies showed that microwave heating at a high power level can cause greater AA formation in products than conventional food heat treatment. The higher content of acrylamide in microwave-heated foods may be due to differences in its formation during microwave heating and conventional methods. At the same time, short exposure to microwaves (during blanching and thawing) at low power may even limit the formation of acrylamide during the final heat treatment. Considering the possible harmful effects of microwave heating on food quality (e.g., intensive formation of acrylamide), further research in this direction should be carried out.

Mycotoxin patulin in food matrices: occurrence and its biological degradation strategies.

Patulin is a mycotoxin produced by a number of filamentous fungal species. It is a polyketide secondary metabolite which can gravely cause human health problems and food safety issues. This review deals with the occurrence of patulin in major food commodities from 2008 to date, including historical aspects, source, occurrence, regulatory limits and its toxicity. Most importantly, an overview of the recent research progress about the biodegradation strategies for contaminated food matrices is provided. The physical and chemical approaches have some drawbacks such as safety issues, possible losses in the nutritional quality, chemical hazards, limited efficacy, and high cost. The biological decontamination based on elimination or degradation of patulin using yeast, bacteria, and fungi has shown good results and it seems to be attractive since it works under mild and environment-friendly conditions. Further studies are needed to make clear the detoxification pathways by available potential biosorbents and to determine the practical applications of these methods at a commercial level to remove patulin from food products with special reference to their effects on sensory characteristics of foods.

Consumer interest in information regarding novel food technologies in Italy: The case of irradiated foods.

Recent food crises and uncertainty regarding food quality have pushed consumers towards a growing need to know more about the foods they purchase, including information related to both food quality and production. This paper identifies the main factors affecting consumer interest in receiving information on food irradiation technology. An online survey was used for research purposes and a total of 392 people, living in Italy, were questioned. Findings revealed that 89.2% of Italian consumers are interested in receiving information on the treatment of foods with ionizing radiation aimed at raising product safety. In particular, this interest was greater for respondents who reported a high sensitivity to food safety issues, who had already or who have unwittingly purchased irradiated foods, and those with a higher level of education. The results also underlined that a problem of misinformation exists among Italian consumers regarding irradiation technology, and that specific food education programs in the schools promoted by the ministry of education and promotional campaigns supported by public and private institutions, aimed at creating greater awareness and attitude among consumers about irradiated products, are necessary.

Vitamin D2 Stability During the Refrigerated Storage of Ultraviolet B-Treated Cultivated Culinary-Medicinal Mushrooms.

The effects of ultraviolet B (UVB) irradiation on the synthesis of vitamin D2 and its stability during refrigerated storage was determined in fresh cultivated culinary-medicinal mushrooms (Agaricus bisporus, Pleurotus ostreatus, and Lentinus edodes) after harvest. The irradiated mushrooms were stored at 4°C for up to 10 days. The concentrations of vitamin D2 and ergosterol were determined using ultrahigh-performance liquid chromatography/tandem mass spectrometry. The cultivated mushrooms not treated with UVB were devoid of vitamin D2. After UVB irradiation, we obtained mushrooms with a large amount of ergocalciferol. A. bisporus showed the lowest vitamin D2 content (3.55 ± 0.11 µg D2/g dry weight); P. ostreatus contained 58.96 ± 1.15 µg D2/g dry weight, and L. edodes contained 29.46 ± 2.21 µg/g dry weight. During storage at 4°C, the amount of vitamin D2 was gradually decreased in P. ostreatus and L. edodes, whereas in A. bisporus vitamin D2 gradually increased until the sixth day, then decreased. Mushrooms exposed to UVB radiation contain a significant amount of vitamin D2 and are therefore an excellent food source of vitamin D.

Compositional Differences of the Winter Culinary-Medicinal Mushroom, Flammulina velutipes (Agaricomycetes), under Three Types of Light Conditions.

The focus of this study was to investigate the effect of light on the cultivation and the amounts of bio-active components in Flammulina velutipes. The mushrooms were cultivated under fluorescent tube (T8) grow lights, lightemitting diodes (LEDs), and cold-cathode fluorescent lamps. The biological efficiency of the T8 lights was the highest, at 92%. The crude fat content, crude fiber content, polysaccharide content, and ergosterol content were highest under the LEDs, at 2.9 g/100 g, 7.9 g/100 g, 3.9 g/100 g, and 1.4 mg/g, respectively. Moreover, vitamin D2 (1.9 µg/g) was generated only under light from LEDs. Principal component analysis showed that F. velutipes cultivated under the 3 different lighting conditions showed different profiles for proximate composition, nutritional compounds, and principal fatty acids.

Radioisotopes produced by neutron irradiation of food.

The use of neutrons for cargo interrogation has the potential to drastically improve threat detection. Previous research has focussed on the production of (24)Na, based on the isotopes produced in pharmaceuticals and medical devices. For both the total activity and the ingestion dose we show that a variety of isotopes contribute and that (24)Na is only dominant under certain conditions. The composition of the foods has a strong influence on the resulting activity and ingestion dose suggesting that the pharmaceuticals and medical devices considered initially are not a viable analogue for foodstuffs. There is an energy dependence to the isotopes produced due to the cross-sections of different reactions varying with neutron energy. We show that this results in different isotopes dominating the ingestion dose at different energies, which has not been considered in the previous literature.

Radiological risks of neutron interrogation of food.

In recent years there has been growing interest in the use of neutron scanning techniques for security. Neutron techniques with a range of energy spectra including thermal, white and fast neutrons have been shown to work in different scenarios. As international interest in neutron scanning increases the risk of activating cargo, especially foodstuffs must be considered. There has been a limited amount of research into the activation of foods by neutron beams and we have sought to improve the amount of information available. In this paper we show that for three important metrics; activity, ingestion dose and Time to Background there is a strong dependence on the food being irradiated and a weak dependence on the energy of irradiation. Previous studies into activation used results based on irradiation of pharmaceuticals as the basis for research into activation of food. The earlier work reports that (24)Na production is the dominant threat which motivated the search for (24)Na(n,γ)(24)Na in highly salted foods. We show that (42)K can be more significant than (24)Na in low sodium foods such as Bananas and Potatoes.

Effect of riboflavin on the photo-oxidative stability of vegetable oil in salad dressing.

Differential scanning calorimetry (DSC), headspace oxygen and solid phase microextraction gas chromatography and peroxide value were used to evaluate the effect of riboflavin on the photo-oxidation of vegetable oil in salad dressing. Salad dressings with 0, 5, 20, 50 and 100 ppm added riboflavin were stored under light (2500 lux) at 25 °C for 5 days. Crystallisation peaks in the DSC thermograms of the oil samples shifted to lower temperatures and enthalpies decreased as the storage time increased. As the riboflavin concentrations increased from 0 to 100 ppm, the crystallisation enthalpies increased from 27 to 31 J/g and the maximum crystallisation temperature increased from -64 to -62 °C during the 5 day storage. Headspace oxygen depletion rates, the formation of volatile compounds and peroxide values of the salad dressing samples simultaneously decreased with the addition of riboflavin, showing that riboflavin protected the oil in salad dressing from photo-oxidation.

Implications of light energy on food quality and packaging selection.

Light energy in the ultraviolet and visible light regions plays a critical role in overall food quality, leading to various degradation and oxidation reactions. Food degradation and oxidation result in the destruction of nutrients and bioactive compounds, the formation of off odors and flavors, the loss of food color, and the formation of toxic substances. Food compounds are sensitive to various light wavelengths. Understanding the effect that specific light wavelengths have on food compounds will allow the development of novel food packaging materials that block the most damaging light wavelengths to photostability of specific food compounds. Future research should focus more specifically on the effect of specific light wavelengths on the quality of specific food products, as there is limited published information on this particular topic. This information also can be directly related to the selection of food packaging materials to retain both high quality and visual clarity of food products exposed to light.

A non-destructive method for quantification the irradiation doses of irradiated sucrose using Vis/NIR spectroscopy.

This article proposes a new method for fast discrimination of irradiation doses of sucrose based on visible-near infrared (Vis/NIR) spectroscopy technology. 250 sucrose samples were categorized into five groups to be irradiated at 0, 1.5, 3.0, 4.5, 6.0 kGy respectively and prepared for the discrimination analysis. The 50 samples of each group were randomly divided into a calibration set containing 40 samples, and a validation set containing the remaining 10 samples. Principal component clustering analysis (PCCA) was applied for the extraction of principal components (PCs) and for clustering analysis. The first five PCs were regarded as the inputs to develop the back propagation neural network (BPNN) model. The performance of the model was validated by the 50 unknown samples and the BPNN achieved an excellent precision and recognition ration of 100%. The results indicated that Vis/NIR spectroscopy could be utilized as a rapid and non-destructive method for the classification of different irradiation doses of irradiated sucrose.

Photodynamic inactivation of methylene blue and tungsten-halogen lamp light against food pathogen Listeria monocytogenes.

The aim of this study was to verify the bactericidal effect and the damage of photodynamic inactivation (PDI) using methylene blue (MB) and tungsten-halogen lamp over Listeria monocytogenes via atomic force microscopy, absorption spectrophotometry, agarose gel electrophoresis, real-time PCR and SDS-PAGE. The obtained data indicated that the viability of L. monocytogenes was ca 7-log reduced by illumination with 10 min tungsten-halogen lamp light under the presence of 0.5 µg mL(-1) MB, and this bactericidal activity against L. monocytogenes of PDI increased proportionally to the concentration of MB and the duration of irradiation. Moreover, after irradiation with MB and visible light, the leakage of intracellular contents was estimated by spectrophotometer at OD(260) and OD(280), which correlated with morphological alterations. Furthermore, genomic DNA cleavage and protein degradation were also detected after PDI treatment. Consequently, breakage of the membrane, damage of the genomic DNA and degradation of bacterial proteins may play an important role in the mechanisms involved in PDI-MB bactericidal activity on L. monocytogenes.

Mechanisms of objectionable textural changes by microwave reheating of foods: a review.

Microwave reheating, compared to a conventional method, is notorious for lack of crust formation and severe toughening of flour and starch-based products. This review discusses how the typical thermal characteristics of microwave heating are involved in affecting the texture as well as the possible role of non-thermal effects. While low surface temperature is the well known mechanism why microwave heating is incapable of crust formation, the most severe toughening problems are caused by internal boiling. Beside moisture loss, the internally generated steam causes 2 main textural effects when it is vented out. The first is the replacing of non-condensable gases (air) in the product voids with a condensable one (steam). When the latter is condensed by cooling, a vacuum may be created in the voids causing their collapse and a formation of a more compact and tougher structure. The second textural effect involves amylose extraction from starch granules and its redistribution to eventually form a rich layer on the walls of the structural foam cells of the baked goods. Relatively fast crystallization of the amylose seems to be the main cause of toughening a short while after microwave heating. This mechanism is relevant mainly to products where starch is an important structural element. Structural disruptions by localize excessive steam pressure at hot-spots are also discussed in this review as well as methods of preventing or alleviating the most objectionable textural changes. The most effective ways of preventing these undesirable changes are by avoiding internal boiling and/or by manipulating the starch content and properties.

Comparison of the migration of melamine from melamine-formaldehyde plastics ('melaware') into various food simulants and foods themselves.

A variety of melaware articles were tested for the migration of melamine into the food simulant 3% w/v acetic acid as a benchmark, and into other food simulants, beverages and foods for comparison. The results indicate that the acidity of the food simulant plays a role in promoting migration, but not by as much as might have been anticipated, since 3% acetic acid gave migration values about double those obtained using water under the same time and temperature test conditions. In contrast, migration into the fatty food simulant olive oil was not detectable and at least 20-fold lower than with the aqueous food simulants. This was expected given the solubility properties of melamine and the characteristics of the melaware plastic. Migration levels into hot acidic beverages (apple juice, tomato juice, red-fruit tea and black coffee) were rather similar to the acetic acid simulant when the same time and temperature test conditions are used, e.g. 2 h at 70°C. However, migration levels into foods that were placed hot into melaware articles and then allowed to cool on standing were much lower (6-14 times lower) than if pre-heated food was placed into the articles and then maintained (artificially) at that high temperature in the same way that a controlled time-temperature test using simulants would be conducted. This very strong influence of time and especially temperature was manifest in the effects seen of microwave heating of food or beverage in the melaware articles. Here, despite the short duration of hot contact, migration levels were similar to simulants used for longer periods, e.g. 70°C for 2 h. This is rationalized in terms of the peak temperature achieved on microwave heating, which may exceed 70°C, counterbalancing the shorter time period held hot. There was also evidence that when using melaware utensils in boiling liquids, as for stovetop use of spatulas, the boiling action of circulating food/simulant can have an additional effect in promoting surface erosion, increasing the plastic decomposition and so elevating the melamine release.

[Hygienic assessment of irradiation doses of Mtskheta-Mtianeti region' population].

Radionuclide content in the food and water in Mtskheta-Mtianeti region of Georgia has been studied, internal and total irradiation doses for the population have been defined and preventive measures for its reduction were detected. Internal irradiation dose for the population due to (40)K was identified as 1,58 mSv/y, total irradiation dose (external and internal irradiation) - 2,57 mSv/y, that is to some extent high than acceptable levels. To reduce the total irradiation dose of a population it is necessary to prohibit the use of high activity water for drinking; provision of the population with new sources of low activity water; minimize and control of irradiation doses from artificial sources including means of regulation of medical radiological procedures and rational use of fertilizers with (40)K content.

Direct chiral determination of free amino acid enantiomers by two-dimensional liquid chromatography: application to control transformations in E-beam irradiated foodstuffs.

Changes in free amino acids content and its potential racemization in ready-to-eat foods treated with E-beam irradiation between 1 and 8 kGy for sanitation purposes were studied. A simple heart cut two-dimensional high performance liquid chromatographic method (LC-LC) for the simultaneous enantiomeric determination of three pairs of amino acids used as markers (tyrosine, phenylalanine, and tryptophan) is presented. The proposed method involves the use of two chromatographs in an LC-LC achiral-chiral coupling. Amino acids and their decomposition products were firstly separated in a primary column (C(18)) using a mixture of ammonium acetate buffer (20 mM, pH 6) (94%) and methanol (6%) as the mobile phase. Then, a portion of each peak was transferred by heart cutting through a switching valve to a teicoplanin-chiral column. Methanol (90%)/water (10%) was used as the mobile phase. Ultraviolet detection was at 260 nm. Detection limits were between 0.16 and 3 mg L(-1) for each enantiomer. Recoveries were in the range 79-98%. The LC-LC method combined with the proposed sample extraction procedure is suitable for complex samples; it involves an online cleanup, and it prevents degradation of protein, racemization of L-enantiomers, and degradation of tryptophan. Under these conditions, D-amino acids were not found in any of the analyzed samples at detection levels of the proposed method.

Assessment of nutrient stability in foods from the space food system after long-duration spaceflight on the ISS.

Maintaining an intact nutrient supply in the food system flown on spacecraft is a critical issue for mission success and crew health. Ground-based evidence indicates that some vitamins may be altered and fatty acids oxidized (and therefore rendered useless, or even dangerous) by long-term storage and by exposure to radiation, both of which will be issues for long-duration exploration missions in space. In this study, the stability of nutrients was investigated in food samples exposed to spaceflight on the Intl. Space Station (ISS). A total of 6 replicates of 5 different space food items, a multivitamin, and a vitamin D supplement were packaged into 4 identical kits and were launched in 2006 on the space shuttle. After 13, 353, 596, and 880 d of spaceflight aboard the ISS, the kits were returned to Earth. Nine replicates of each food item and vitamin, from the same lots as those sent into space, remained in an environmental chamber on Earth to serve as controls at each time point. Vitamins, hexanal, oxygen radical absorbance capacity, and amino acids were measured in identical-lot food samples at each time point. After 596 d of spaceflight, differences in intact vitamin concentrations due to duration of storage were observed for most foodstuffs, but generally, nutrients from flight samples did not degrade any faster than ground controls. This study provided the 1st set of spaceflight data for investigation of nutrient stability in the food system, and the results will help NASA design food systems for both ISS and space exploration missions.

[Radiation on the dining table]. / La radiación a la mesa.

Zero tolerance to bacterial contamination means considering the acceptance of "radiation on the table". The process of food irradiation has been extensively studied, nevertheless its use remains a matter of some controversy. Despite unanimous agreement within the medical community of the safety of this procedure, occasional concerns arise from the consumers. A common consumer misconception is that irradiation may turn the food "radioactive". A significant number of scientific studies on the topic were analyzed. We found no scientific study demonstrating that consumption of irradiated food might pose a risk to consumers. All studies conclude that food irradiation at the appropriate dose required to reduce contamination is safe and does not affect its nutritional value. In order to emphasize the issue we discuss the potential benefit vs harm of irradiation of food contaminated with E. coli 0157: H7. The association of this bacteria with severe disease and death has been clearly established in contrast with the lack of a demonstrated risk due to meat irradiation. We conclude that the risks of food irradiation remains "unknown" simply because, after four decades of research, none has been identified. In contrast to the risks of acquiring a food transmitted bacterial disease, the risk of irradiation is negligible.

La radiación a la mesa / Radiation on the dining table

Zero tolerance to bacterial contamination means considering the acceptance of "radiation on the table". The process of food irradiation has been extensively studied, nevertheless its use remains a matter of some controversy. Despite unanimous agreement within the medical community of the safety of this procedure, occasional concerns arise from the consumers. Acommon consumer misconception is that irradiationmay turn the food "radioactive". A significant number of scientific studies on the topic were analyzed. We found no scientific study demonstrating that consumption of irradiated food might pose a risk to consumers. All studies conclude that food irradiation at the appropriate dose required to reduce contamination is safe and does not affect its nutritional valué. In order to emphasize the issue we discuss the potential benefit vs harm of irradiation of food contaminated with E. coli 0157: H7. The association of this bacteria with severe disease and death has been clearly established in contrast with the lack of a demonstrated risk due to meat irradiation. We conclude that the risks of food irradiation remains "unknown" simply because, after four decades of research, none has been identified. In contrast to the risks of acquiring a food transmitted bacterial disease, the risk of irradiation is negligible.

Tolerancia cero a la contaminación bacteriana implica considerar la inclusión de "la radiación a la mesa". La irradiación es uno de los métodos de procesado de alimentos que se han estudiado de forma más extensa y estricta; sin embargo, su aplicación sigue siendo materia de discusión. Incluso si la inocuidad está bien establecida (hay acuerdo unánime entre la comunidad científica), de vez en cuando surgen preguntas de los consumidores. Es importante destacar que no hay ningún estudio científico consistente y reproducible, que demuestre o sugiera que el consumo de un alimento irradiado podría suponer un riesgo para la salud de los consumidores. Creemos que esta revisión es un aporte importante al conocimiento. Es necesario desmentir los errores generalizados sobre la irradiación, especialmente la idea de que los alimentos se tornan radioactivos. Se analizó una cantidad considerable de investigaciones científicas difundidas en publicaciones especializadas. De ellas surge que los alimentos irradiados con la dosis necesaria para alcanzar el objetivo mi-crobiológico deseado, se consideran inocuos y saludables para el consumo y adecuados desde un punto de vista nutricional. Para ser más claros, nadie pondría en duda que Escherichia coli 0157: H7 puede causar la muerte, la carne irradiada no. En definitiva, los riesgos de la irradiación de los alimentos son desconocidos, simplemente porque después de cuatro décadas de investigaciones, no se ha encontrado ninguno. Este es un argumento de suficiente peso, contra los riesgos conocidos de contraer una enfermedad bacteriana transmitida por alimentos.