1.
Res Exp Med (Berl)
; 181(2): 105-12, 1982.
Article
in English
| MEDLINE
| ID: mdl-6817397
ABSTRACT
Determination of proteolytic activities gains increasing importance for the diagnosis of various pathologic conditions. Many questions can only be solved with the aid of experiments with animals. In preparation of such studies the pattern of the enzymatic hydrolysis of 20 amino acid-p-nitroanilides and one -beta-naphthylamide by normal rat serum was measured. The pH dependences of the single reactions are reported as well as their sensitivity to the action of ethylenediaminetetraacetic acid, dithioerithrol, and puromycin. Additionally, it is shown that collecting of the blood by cardiac puncture and preparation of the native plasma is the best way to get reproducible results.
Subject(s)
Amino Acids/pharmacology , Formamides/pharmacology , Peptide Hydrolases/blood , Amino Acids/antagonists & inhibitors , Animals , Blood Coagulation , Clinical Enzyme Tests/methods , Dipeptides/metabolism , Dithioerythritol/pharmacology , Edetic Acid/pharmacology , Formamides/antagonists & inhibitors , Hemolysis , Hydrogen-Ion Concentration , Hydrolysis , Puromycin/pharmacology , Rats , Wound Healing
2.
Environ Qual Saf Suppl
; 3: 401-5, 1975.
Article
in English
| MEDLINE
| ID: mdl-773643
Subject(s)
Formamides/toxicity , Pesticides/toxicity , Ticks/physiology , Animals , Chlorphenamidine/metabolism , Drug Resistance , Drug Synergism , Female , Formamides/antagonists & inhibitors , Formamides/metabolism , Larva/metabolism , Motor Activity/drug effects , Pesticide Synergists/metabolism , Piperonyl Butoxide/metabolism , Stimulation, Chemical , Structure-Activity Relationship , Ticks/metabolism
3.
Biochem Biophys Res Commun
; 44(6): 1457-63, 1971 Sep 17.
Article
in English
| MEDLINE
| ID: mdl-5160705
Subject(s)
Amides/pharmacology , Anti-Inflammatory Agents/pharmacology , Azo Compounds/pharmacology , Lysosomes/drug effects , Acid Phosphatase , Adenosine Triphosphate/pharmacology , Animals , Aspirin/pharmacology , Azo Compounds/antagonists & inhibitors , Chloroquine/pharmacology , Drug Antagonism , Flufenamic Acid/pharmacology , Formamides/antagonists & inhibitors , Formamides/pharmacology , Glucuronidase , Hexosaminidases , Hydrocortisone/pharmacology , In Vitro Techniques , Indomethacin/pharmacology , Liver/drug effects , Lysosomes/enzymology , Male , Mefenamic Acid/pharmacology , Membranes/drug effects , Phenylbutazone/pharmacology , Rats , Sulfatases
4.
J Pharmacol Exp Ther
; 177(1): 301-8, 1971 Apr.
Article
in English
| MEDLINE
| ID: mdl-5566771
Subject(s)
Sulfones/therapeutic use , Aminobenzoates/pharmacology , Animals , Drug Resistance, Microbial , Female , Formamides/antagonists & inhibitors , Formamides/therapeutic use , Injections, Intraperitoneal , Injections, Intravenous , Malaria/drug therapy , Male , Mice , Sulfadiazine/pharmacology , Sulfones/administration & dosage , Sulfones/antagonists & inhibitors , Sulfones/blood , Time Factors
5.
Biochim Biophys Acta
; 232(1): 48-60, 1971 Feb 25.
Article
in English
| MEDLINE
| ID: mdl-4930205
