Plasticity in central olfactory processing and pheromone blend discrimination following interspecies antennal imaginal disc transplantation.

The antennal imaginal disc was transplanted between premetamorphic male larvae of two different Lepidopteran moth species. Following adult eclosion, electrophysiological recordings were made from 33 central olfactory neurons in the antennal lobes of both Helicoverpa zea donor to Heliothis virescens recipient (Z-V) and reciprocal (V-Z) transplants. Under the influence of sensory neuron input derived from the transplanted antennal imaginal disc, most antennal lobe projection neurons (29/33) were classified as belonging to physiological categories encountered previously in donor species males. Furthermore, when stained many of these neurons had dendritic arbors restricted to donor-induced glomerular locations predicted by their physiology. However, some neurons with unexpected physiological profiles were also identified (4/33), but only in V-Z transplants. These profiles help to explain why some V-Z bilateral transplants were able to respond to both pheromone blends in flight tunnel bioassays, an unforeseen result counter to the assumption that a donor antenna develops a normal donor antennal olfactory receptor neuron complement. Stainings of several neurons in V-Z transplant males also revealed unusual morphological features including multiglomerular dendritic arbors and "incorrect" glomerular locations. These results indicate a developmental plasticity in the final dendritic arborization pattern of central olfactory neurons, including an ability to colonize and integrate inputs across topographically novel donor glomeruli, different from those found in the normal recipient antennal lobe.

Development of neuronal ganglion xenografts from gastropoda in rat brain.

Survival of neuronal ganglia from newborn snail (Helix aspera L.) in the brain of adult rats was studied. Snail ganglion survived in the brain of warm-blooded animals for 6 months without inducing immune conflict. At early stages (5 days) after transplantation, xenografts increased in size and were several times larger than native ganglia from 10-day-old snails, thereafter (on days 28 and 180) they became smaller still surpassing the sizes of ganglia from snail of the corresponding age. Rapid enlargement of the xenograft was due to cell reactive processes in the ganglion. Deep penetration of large vessels from xenografts to rat brain was observed.

Regeneration of phasic synapses on a crayfish slow muscle following allotransplantation of a mixed phasic-tonic nerve.

Separate phasic or tonic nerves allotransplanted to reinnervate a denervated slow superficial flexor muscle (SFM) in the abdomen of adult crayfish regenerate synaptic nerve terminals with phasic or tonic properties. To test competitive interactions between tonic and phasic axons, we allotransplanted the sixth abdominal ganglion with its third nerve root containing a mixture of phasic and tonic axons onto the denervated SFM. The resulting reinnervation of the SFM was compared to the normal innervation on the contralateral intact SFM, which receives innervation only from tonic motoneurons. Variable sizes of excitatory postsynaptic potentials indicated that 2-3 axons innervated each muscle fiber of the SFM in both the allotransplant and normal preparations. Compared to the normal tonic terminals on the intact contralateral side, the allotransplanted synaptic terminals had more phasic-like properties; specifically, they gave rise to larger synaptic potentials, had a lower mitochondrial content and contained a higher density of active zone dense bars per synapse. Moreover, prolific sprouting of the axons in the regenerated nerve, typical of phasic axons, points to more vigorous regeneration of phasic rather than tonic axons to the denervated SFM. In keeping with this prolific axon sprouting, there was both a much higher density of innervation in the allotransplanted SFM compared to the normal SFM, and a higher frequency of extrasynaptic active zones in regenerated terminals of the mixed nerve compared to those of the tonic nerve. Thus, an allotransplanted mixed nerve regenerates mainly phasic axons and synapses on the slow denervated SFM, demonstrating the instructive nature of the neuron in synapse specification, as well as the permissive nature of the target muscle.

[Effect of dopamine enriched ganglion xenografts from the immature CNS of Helix aspersa L. snail on the learning time during acquisition of an operant food-procuring reflex in WAG/RIJ rats]. / Vliianie ksenotransplantatov gangliev, bogatykh dofaminom, iz nezreloi TsNS molliuska Helix aspersa L. na vremia obucheniia pri vyrabotke instrumental'nogo pishchedobyvatel'nogo refleksa u krys linii WAG/RIJ.

Xenografts from the ganglia of a newborn terrestrial snail Helix aspersa L. were implanted into the right parietal area of the brain cortex of WAG/Rij rats with absence epilepsy. Rats with implanted xenografts were trained for reaching a food ball from a tube (reaching test). It was shown that the mean duration of each leaning stage and total time necessary for acquisition of the instrumental conditioning (till the learning criterion) were shorter in animals with xenografts than in control groups of animals.

Structure of allotransplanted ganglia and regenerated neuromuscular connections in crayfish.

In adult crayfish, Procambarus clarkii, motoneurons to a denervated abdominal superficial flexor muscle regenerate long-lasting and highly specific synaptic connections as seen from recordings of excitatory postsynaptic potentials, even when they arise from the ganglion of another crayfish. To confirm the morphological origins of these physiological connections we examined the fine structure of the allotransplanted tissue that consisted of the third abdominal ganglion and the nerve to the superficial flexor muscle (the fourth ganglion and the connecting ventral nerve cord were also included). Although there is considerable degeneration, the allotransplanted ganglia display intact areas of axon tracts, neuropil, and somata. Thus in both short (6-8 weeks) and long (24-30 weeks) term transplants approximately 20 healthy somata are present and this is more than the five axons regenerated to the host muscle. The principal neurite and dendrites of these somata receive both excitatory and inhibitory synaptic inputs, and these types of synaptic contacts also occur among the dendritic profiles of the neuropil. Axon tracts in the allotransplanted ganglia and ventral nerve cord consist largely of small diameter axons; most of the large axons including the medial and lateral giant axons are lost. The transplanted ganglia have many blood vessels and blood lacunae ensuring long-term survival. The transplanted superficial flexor nerve regenerates from the ventral to the dorsal surface of the muscle where it has five axons, each consisting of many profiles rather than a single profile. This indicates sprouting of the individual axons and accounts for the enlarged size of the regenerated nerve. The regenerated axons give rise to normal-looking synaptic terminals with well-defined synaptic contacts and presynaptic dense bars or active zones. Some of these synaptic terminals lie in close proximity to degenerating terminals, suggesting that they may inhabit old sites and in this way ensure target specificity. The presence of intact somata, neuropil, and axon tracts are factors that would contribute to the spontaneous firing of the transplanted motoneurons.

Allogeneic and xenogeneic grafts in pulmonate gastropod molluscs: fates of neural transplants.

Neural intracerebral allo- and xenografts in pulmonate gastropods demonstrated a variation in the tolerance of neural xenogeneic grafts that was dependent on the phylogenetic distance between the donor and the host. Like allografts, neural congeneric xenografts (Hp/Haa and H1/Haa) of cerebral ganglia (CG) were tolerated and restored growth in juvenile mesocerebrum-deprived (Haa) snails. However, CG neural xenografts between different genera of stylommatophorans (Achatina fulica/Haa) or between genera of different orders (Lymnaea stagnalis: Basommatophora/Haa: Stylommatophora) revealed an interspecific histoincompatibility. These results, compared with those described by other authors, suggest that gastropods possess mechanisms for the recognition of non-self that depend on the organ considered and the phylogenetic distance separating host and donor. Research should now attempt to identify the factors responsible for graft destruction.

Brain grafts of cerebral ganglia have effectiveness in growth restoration of damaged Helix aspersa mesocerebrum.

The microsurgical extirpation of the mesocerebrum from the brain of fast-growing juvenile snails (Helix aspersa aspersa: H.a.a.) stops their growth. This suggests that neurosecretory cells of the mesocerebrum secrete a growth hormone. Neural grafting has been used as a tool to restore the impaired growth function after mesocerebrum removal in juvenile H.a.a snails. The transplantation of desheathed cerebral ganglia (CG) (i.e. CG with their glioconjunctive outer covering removed), into the place where the mesocerebrum had been re-established growth which depended on the age of the donors. For the grafts of H.a.a CG into H.a.a, it was CG from the youngest donors that restored growth best. However, the CG of adult snails still conserved a slight growth-stimulating activity. Transplantation of the CG from the large, fast-growing sub-species H. aspersa maxima (H.a.m), into the brain of H.a.a with mesocerebrum removed induced faster growth than the H.a.a CG probably because of a more abundant secretion of growth hormone. Our results show that intracerebral CG grafts are well tolerated in snails and that labeling of the neurones of the transplanted CG with a vital fluorescent stain (Fast blue), allowed the observation, over several months, of their integration into the lesion zone of the host brain.

[Influence of brain grafts on growth restoration of snail (Helix aspersa) deprived of the midbrain]. / Influence de greffes cérébrales dans la restauration de la croissance de l'escargot (Helix aspersa) privé de mesocerebrum.

Microsurgical removal of the mesocerebrum from the brain of juvenile snails stopped their growth whereas intracerebral implantation of desheathed cerebral ganglia (CG) re-established it. When the animals were grafted with CG from very young snails growth was much more stimulated than with CG from donors of the same age or from adults. Furthermore, young CG of juvenile fast growing specimens of the large species (Helix aspersa maxima) induced a higher growth rate than the CG of the ordinary small garden snails (Helix aspersa aspersa). Labelling of the neural grafts with the vital fluorescent dye fast blue enabled us to follow the repopulation of the lesioned area of the brain of the host during the functional integration of the implanted neurons into the circuits that control growth in snails.