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2.
Ann N Y Acad Sci ; 1506(1): 118-141, 2021 12.
Article in English | MEDLINE | ID: mdl-34791665

ABSTRACT

The human transcriptome contains many types of noncoding RNAs, which rival the number of protein-coding species. From long noncoding RNAs (lncRNAs) that are over 200 nucleotides long to piwi-interacting RNAs (piRNAs) of only 20 nucleotides, noncoding RNAs play important roles in regulating transcription, epigenetic modifications, translation, and cell signaling. Roles for noncoding RNAs in disease mechanisms are also being uncovered, and several species have been identified as potential drug targets. On May 11-14, 2021, the Keystone eSymposium "Noncoding RNAs: Biology and Applications" brought together researchers working in RNA biology, structure, and technologies to accelerate both the understanding of RNA basic biology and the translation of those findings into clinical applications.


Subject(s)
Congresses as Topic/trends , Epigenesis, Genetic/genetics , Gene Targeting/trends , RNA, Untranslated/administration & dosage , RNA, Untranslated/genetics , Research Report , Animals , Drug Delivery Systems/methods , Drug Delivery Systems/trends , Gene Targeting/methods , Humans , MicroRNAs/administration & dosage , MicroRNAs/genetics , RNA, Long Noncoding/administration & dosage , RNA, Long Noncoding/genetics , RNA, Small Interfering/administration & dosage , RNA, Small Interfering/genetics , RNA, Small Untranslated/administration & dosage , RNA, Small Untranslated/genetics , Signal Transduction/genetics
3.
Ann N Y Acad Sci ; 1506(1): 98-117, 2021 12.
Article in English | MEDLINE | ID: mdl-34786712

ABSTRACT

Synthetic biology has the potential to transform cell- and gene-based therapies for a variety of diseases. Sophisticated tools are now available for both eukaryotic and prokaryotic cells to engineer cells to selectively achieve therapeutic effects in response to one or more disease-related signals, thus sparing healthy tissue from potentially cytotoxic effects. This report summarizes the Keystone eSymposium "Synthetic Biology: At the Crossroads of Genetic Engineering and Human Therapeutics," which took place on May 3 and 4, 2021. Given that several therapies engineered using synthetic biology have entered clinical trials, there was a clear need for a synthetic biology symposium that emphasizes the therapeutic applications of synthetic biology as opposed to the technical aspects. Presenters discussed the use of synthetic biology to improve T cell, gene, and viral therapies, to engineer probiotics, and to expand upon existing modalities and functions of cell-based therapies.


Subject(s)
Congresses as Topic/trends , Genetic Engineering/trends , Genetic Therapy/trends , Research Report , Synthetic Biology/trends , Animals , Cell- and Tissue-Based Therapy/methods , Cell- and Tissue-Based Therapy/trends , Gene Targeting/methods , Gene Targeting/trends , Genetic Engineering/methods , Genetic Therapy/methods , Humans , Killer Cells, Natural/immunology , Machine Learning/trends , Synthetic Biology/methods , T-Lymphocytes/immunology
4.
Ann N Y Acad Sci ; 1506(1): 35-54, 2021 12.
Article in English | MEDLINE | ID: mdl-34435370

ABSTRACT

Facing the challenges of the world's food sources posed by a growing global population and a warming climate will require improvements in plant breeding and technology. Enhancing crop resiliency and yield via genome engineering will undoubtedly be a key part of the solution. The advent of new tools, such as CRIPSR/Cas, has ushered in significant advances in plant genome engineering. However, several serious challenges remain in achieving this goal. Among them are efficient transformation and plant regeneration for most crop species, low frequency of some editing applications, and high attrition rates. On March 8 and 9, 2021, experts in plant genome engineering and breeding from academia and industry met virtually for the Keystone eSymposium "Plant Genome Engineering: From Lab to Field" to discuss advances in genome editing tools, plant transformation, plant breeding, and crop trait development, all vital for transferring the benefits of novel technologies to the field.


Subject(s)
Congresses as Topic , Crops, Agricultural/genetics , Genetic Engineering/methods , Genome, Plant/genetics , Plant Breeding/methods , Research Report , CRISPR-Cas Systems/genetics , Congresses as Topic/trends , Gene Editing/methods , Gene Editing/trends , Gene Targeting/methods , Gene Targeting/trends , Genetic Engineering/trends
5.
Biochem Pharmacol ; 189: 114357, 2021 07.
Article in English | MEDLINE | ID: mdl-33279497

ABSTRACT

Pancreatic Cancer (PC) is a severe form of malignancy all over the world. Delayed diagnosis and chemoresistance are the major factors contributing to its poor prognosis and high mortality rate. The genetic and epigenetic regulations of biological pathways further complicate the progression and chemotherapy response to this cancer. MicroRNAs (MiRNAs) involvement has been observed in all types of cancers including PC. The understanding and categorization of miRNAs according to their specific targets are very important to develop early diagnostic and therapeutic interventions. The current review, emphasizing recent research findings, has categorized miRNAs that directly target the potential onco-factors that act as central converging signal-nodes in five major cancer-related pathways i.e., MAPK/ERK, JAK/STAT, Wnt/ß-catenin, AKT/mTOR, and TGFß in PC. The therapeutic perspectives of miRNAs in PC have also been discussed. This will help to understand the interplay of various miRNAs within foremost signaling pathways and develop a multifactorial approach to treat difficult-to-treat PC.


Subject(s)
Gene Targeting/methods , MicroRNAs/administration & dosage , MicroRNAs/metabolism , Pancreatic Neoplasms/metabolism , Pancreatic Neoplasms/therapy , Animals , Gene Targeting/trends , Humans , Inflammation Mediators/antagonists & inhibitors , Inflammation Mediators/metabolism , MAP Kinase Signaling System/drug effects , MAP Kinase Signaling System/physiology , MicroRNAs/genetics , Pancreatic Neoplasms/genetics , Signal Transduction/drug effects , Signal Transduction/physiology , TOR Serine-Threonine Kinases/antagonists & inhibitors , TOR Serine-Threonine Kinases/genetics , TOR Serine-Threonine Kinases/metabolism
6.
Biochem Pharmacol ; 189: 114301, 2021 07.
Article in English | MEDLINE | ID: mdl-33203517

ABSTRACT

Despite considerable investigation in diabetic nephropathy (DN) pathogenesis and possible treatments, current therapies still do not provide competent prevention from disease progression to end-stage renal disease (ESRD) in most patients. Therefore, investigating exact molecular mechanisms and important mediators underlying DN may help design better therapeutic approaches for proper treatment. MicroRNAs (MiRNAs) are a class of small non-coding RNAs that play a crucial role in post-transcriptional regulation of many gene expression within the cells and present an excellent opportunity for new therapeutic approaches because their profile is often changed during many diseases, including DN. This review discusses the most important signaling pathways involved in DN and changes in miRNAs profile in each signaling pathway. We also suggest possible approaches for miRNA derived interventions for designing better treatment of DN.


Subject(s)
Diabetic Nephropathies/metabolism , Diabetic Nephropathies/therapy , Gene Targeting/trends , MicroRNAs/administration & dosage , MicroRNAs/metabolism , Animals , Diabetes Mellitus/drug therapy , Diabetes Mellitus/genetics , Diabetes Mellitus/metabolism , Diabetic Nephropathies/genetics , Gene Targeting/methods , Humans , MicroRNAs/genetics , Signal Transduction/drug effects , Signal Transduction/physiology , Transforming Growth Factor beta/antagonists & inhibitors , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/metabolism , Treatment Outcome
7.
Epilepsia ; 61(10): 2086-2096, 2020 10.
Article in English | MEDLINE | ID: mdl-32944964

ABSTRACT

Epilepsy is a chronic brain dysfunction. Current antiepileptic medicines cannot prevent epileptogenesis. Increasing data have shown that microRNAs (miRNAs) are selectively altered within the epileptic hippocampi of experimental models and human tissues, and these alterations affect the genes that control epileptogenesis. Furthermore, manipulation of miRNAs in animal models can modify epileptogenesis. As a result, miRNAs have been proposed as promising targets for treating epilepsy. We searched PubMed using the terms "microRNAs/miRNAs AND epilepsy", "microRNAs/miRNAs AND epileptogenesis", and "microRNAs/miRNAs AND seizure". We selected the articles in which the relationship between miRNAs and target gene(s) was validated and manipulation of miRNAs in in vivo epilepsy models modified epileptogenesis during the chronic phase via gene regulation. A total of 13 miRNAs were found in the present review. Based on the current analysis of miRNAs and their target gene(s), each miRNA has limitations as a potential epilepsy target. Importantly, miR-211 or miR-128 transgenic mice displayed seizures. These findings highlight new developments for epileptogenesis prevention. Developing novel strategies to modify epileptogenesis will be effective in curing epilepsy patients. This article provides an overview of the clinical application of miRNAs as novel targets for epilepsy.


Subject(s)
Epilepsy/genetics , Epilepsy/therapy , Gene Targeting/methods , Genetic Therapy/methods , MicroRNAs/genetics , Animals , Epilepsy/metabolism , Gene Targeting/trends , Genetic Therapy/trends , Humans , MicroRNAs/metabolism
8.
Nat Rev Neurol ; 16(9): 506-519, 2020 09.
Article in English | MEDLINE | ID: mdl-32546757

ABSTRACT

Seizures result from hypersynchronous, abnormal firing of neuronal populations and are the primary clinical symptom of the epilepsies. Brain tissue from animal models and patients with acquired forms of epilepsy commonly features selective neuronal loss, gliosis, inflammatory markers and microscopic and macroscopic reorganization of networks. The gene expression landscape is a critical driver of these changes, and gene expression is fine tuned by small, non-coding RNAs called microRNAs (miRNAs). miRNAs inhibit the function of protein-coding transcripts, resulting in changes in multiple aspects of cell structure and function, including axonal and dendritic structure and the repertoire of neurotransmitter receptors, ion channels and transporters that establish neurophysiological functions. Dysregulation of the miRNA system has emerged as a mechanism that underlies epileptogenesis. Given that miRNAs can act on multiple mRNA targets, their manipulation offers a novel, multi-targeting approach to correct disturbed gene expression patterns. Targeting of some miRNAs has also been used to selectively upregulate individual transcripts, offering the possibility of precision therapy approaches for disorders of haploinsufficiency. In this Review, we discuss how miRNAs determine and control neuronal and glial functions, how this process is altered in states associated with hyperexcitability, and the prospects for miRNA targeting for the treatment of epilepsy.


Subject(s)
Epilepsy/genetics , Epilepsy/therapy , Gene Targeting/trends , MicroRNAs/genetics , Animals , Clinical Trials as Topic/methods , Epilepsy/metabolism , Gene Targeting/methods , Humans , MicroRNAs/administration & dosage , MicroRNAs/metabolism , Neuroglia/physiology , Neurons/physiology , Treatment Outcome
9.
Biochem Pharmacol ; 174: 113831, 2020 04.
Article in English | MEDLINE | ID: mdl-32004549

ABSTRACT

Innate sensing of viruses by cytosolic nucleic acid sensors is a key feature of anti-viral immunity against these pathogens. The DNA sensing pathway through the sensor cyclic GMP-AMP synthase (cGAS) and its downstream effector stimulator of interferon genes (STING) has emerged in recent years as a key, front-line means of driving interferons and pro-inflammatory cytokines in response to DNA virus infection in vertebrates. Unsurprisingly, many DNA viruses have evolved effective inhibitors of this signalling system which target at a wide variety of points from sensing all the way down to the activation of Interferon Regulatory Factor (IRF)-family and Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)-family transcription factors which drive a program of pro-inflammatory and anti-viral gene expression. Here we review DNA viruses that have been shown to inhibit this pathway and the inhibitors they have evolved to do it.


Subject(s)
DNA Viruses/metabolism , Gene Targeting/methods , Membrane Proteins/metabolism , Nucleotidyltransferases/metabolism , Animals , DNA Viruses/genetics , DNA Viruses/immunology , DNA, Viral/genetics , DNA, Viral/immunology , DNA, Viral/metabolism , Gene Targeting/trends , Humans , Immunity, Innate/physiology , Inflammation Mediators/immunology , Inflammation Mediators/metabolism , Membrane Proteins/genetics , Membrane Proteins/immunology , Nucleotidyltransferases/genetics , Nucleotidyltransferases/immunology
10.
J Oncol Pharm Pract ; 26(4): 943-966, 2020 Jun.
Article in English | MEDLINE | ID: mdl-31964218

ABSTRACT

Lymphomas are a diverse group of hematologic malignancies that arise from either T cell, B cell or the natural killer cell lineage. B cell lymphomas arise from gene mutations with critical functions during normal B cell development. Recent advances in the understanding of molecular pathogenesis demonstrate that many different recurrent genomic and molecular abnormalities and dysregulated oncogenic regulatory pathways exist for many subtypes of B cell lymphomas, both across and within histological subtypes. Pathogenetic processes such as (1) chromosomal aberrations, for example, t(14;18) in follicular lymphoma, t(11;14) in mantle cell lymphoma, t(8;14) in Burkitt lymphoma; dysregulations in signaling pathways of (2) nuclear factor- κB (NF-κB); (3) B cell receptor (BCR); (4) Janus kinase/signal transducers and transcription activators (JAK-STAT); (5) impaired apoptosis/cell cycle regulation due to mutated, rearranged or amplified MYC, BCL-2, BCL-6 proto-oncogenes; (6) epigenetic aberrations may contribute to pathogenesis. More studies are under way to elucidate the molecular heterogeneity underlying many types of lymphomas that account for variable responses to treatment, generation of subclones and treatment resistance. Although significant research is still needed, targeted therapy promises to provide new options for the treatment of patients with lymphomas. This article provides a non-exhaustive overview on the current understanding on the genetics of pathogenesis of B cell lymphomas and their therapeutic implications.


Subject(s)
Biomarkers, Tumor/genetics , Lymphoma, B-Cell/genetics , Lymphoma, B-Cell/therapy , Precision Medicine/methods , Biomarkers, Tumor/antagonists & inhibitors , Biomarkers, Tumor/metabolism , Drug Delivery Systems/methods , Drug Delivery Systems/trends , Gene Targeting/methods , Gene Targeting/trends , Humans , Lymphoma, B-Cell/metabolism , Lymphoma, Follicular/genetics , Lymphoma, Follicular/metabolism , Lymphoma, Follicular/therapy , Lymphoma, Non-Hodgkin/genetics , Lymphoma, Non-Hodgkin/metabolism , Lymphoma, Non-Hodgkin/therapy , Mutation/drug effects , Mutation/physiology , Precision Medicine/trends , Proto-Oncogene Proteins c-bcl-2/antagonists & inhibitors , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Signal Transduction/drug effects , Signal Transduction/physiology
11.
Cell Mol Life Sci ; 76(24): 4861-4867, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31367845

ABSTRACT

RNAs are responsible for mediating genetic information flow within the cell. RNA splicing, modification, trafficking, translation, and stability are all controlled at the transcript level. However, biological tools to study and manipulate them in a programmable fashion are currently limited. In this review, we summarize recent advances regarding available RNA-targeting systems discovered so far, including CRISPR-based technologies-Cas9 and Cas13, and programmable RNA-binding proteins-PUF and PPR. These tools allow transcript-specific manipulation in gene expression.


Subject(s)
RNA, Guide, Kinetoplastida/genetics , RNA-Binding Proteins/genetics , RNA/genetics , CRISPR-Cas Systems/genetics , Gene Editing , Gene Targeting/trends , Humans
13.
Neurotherapeutics ; 16(4): 1074-1096, 2019 10.
Article in English | MEDLINE | ID: mdl-31432449

ABSTRACT

Spinocerebellar ataxia type 7 (SCA7) is a rare autosomal dominant neurodegenerative disorder characterized by progressive neuronal loss in the cerebellum, brainstem, and retina, leading to cerebellar ataxia and blindness as major symptoms. SCA7 is due to the expansion of a CAG triplet repeat that is translated into a polyglutamine tract in ATXN7. Larger SCA7 expansions are associated with earlier onset of symptoms and more severe and rapid disease progression. Here, we summarize the pathological and genetic aspects of SCA7, compile the current knowledge about ATXN7 functions, and then focus on recent advances in understanding the pathogenesis and in developing biomarkers and therapeutic strategies. ATXN7 is a bona fide subunit of the multiprotein SAGA complex, a transcriptional coactivator harboring chromatin remodeling activities, and plays a role in the differentiation of photoreceptors and Purkinje neurons, two highly vulnerable neuronal cell types in SCA7. Polyglutamine expansion in ATXN7 causes its misfolding and intranuclear accumulation, leading to changes in interactions with native partners and/or partners sequestration in insoluble nuclear inclusions. Studies of cellular and animal models of SCA7 have been crucial to unveil pathomechanistic aspects of the disease, including gene deregulation, mitochondrial and metabolic dysfunctions, cell and non-cell autonomous protein toxicity, loss of neuronal identity, and cell death mechanisms. However, a better understanding of the principal molecular mechanisms by which mutant ATXN7 elicits neurotoxicity, and how interconnected pathogenic cascades lead to neurodegeneration is needed for the development of effective therapies. At present, therapeutic strategies using nucleic acid-based molecules to silence mutant ATXN7 gene expression are under development for SCA7.


Subject(s)
Ataxin-7/genetics , Disease Models, Animal , Drug Delivery Systems/trends , Gene Targeting/trends , Spinocerebellar Ataxias/genetics , Spinocerebellar Ataxias/therapy , Animals , Ataxin-7/metabolism , Autophagy/physiology , Brain/metabolism , Brain/pathology , Drug Delivery Systems/methods , Gene Targeting/methods , Genetic Therapy/methods , Genetic Therapy/trends , Humans , Neurons/metabolism , Neurons/pathology , Peptides/genetics , Peptides/metabolism , Spinocerebellar Ataxias/metabolism
14.
Neurotherapeutics ; 16(4): 999-1008, 2019 10.
Article in English | MEDLINE | ID: mdl-31338702

ABSTRACT

The spinocerebellar ataxias (SCAs) are a group of neurodegenerative disorders inherited in an autosomal dominant fashion. The SCAs result in progressive gait imbalance, incoordination of the limbs, speech changes, and oculomotor dysfunction, among other symptoms. Over the past few decades, significant strides have been made in understanding the pathogenic mechanisms underlying these diseases. Although multiple efforts using a combination of genetics and pharmacology with small molecules have been made towards developing new therapeutics, no FDA approved treatment currently exists. In this review, we focus on SCA1, a common SCA subtype, in which some of the greatest advances have been made in understanding disease biology, and consequently potential therapeutic targets. Understanding of the underlying basic biology and targets of therapy in SCA1 is likely to give insight into treatment strategies in other SCAs. The diversity of the biology in the SCAs, and insight from SCA1 suggests, however, that both shared treatment strategies and specific approaches tailored to treat distinct genetic causes of SCA are likely needed for this group of devastating neurological disorders.


Subject(s)
Ataxin-1/genetics , Clinical Trials as Topic/methods , Drug Delivery Systems/trends , Gene Targeting/trends , Spinocerebellar Ataxias/genetics , Spinocerebellar Ataxias/therapy , Animals , Ataxin-1/antagonists & inhibitors , Ataxin-1/metabolism , Drug Delivery Systems/methods , Excitatory Amino Acid Agents/administration & dosage , Excitatory Amino Acid Agents/metabolism , Gene Targeting/methods , Genetic Therapy/methods , Genetic Therapy/trends , Humans , Oligonucleotides, Antisense/administration & dosage , Oligonucleotides, Antisense/genetics , Oligonucleotides, Antisense/metabolism , RNA Interference/drug effects , RNA Interference/physiology , Spinocerebellar Ataxias/metabolism
15.
Mol Neurobiol ; 56(12): 8101-8108, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31183807

ABSTRACT

Disruptions in multiple neurobiological pathways and neuromolecular processes have been widely implicated in the etiopathology of Alzheimer's disease (AD), a complex, progressive, and ultimately lethal neurological disorder whose current incidence, both domestically and globally, is reaching epidemic proportions. While only a few percent of all AD cases appear to have a strong genetic or familial component, the major form of this disease, known as idiopathic or sporadic AD, displays a multi-factorial pathology and represents one of the most complex and perplexing neurological disorders known. More effective and innovative pharmacological strategies for the successful intervention and management of AD might be expected: (i) to arise from strategic-treatments that simultaneously address multiple interrelated AD targets that are directed at the initiation, development, and/or propagation of this disease and (ii) those that target the "neuropathological core" of the AD process at early or upstream stages of AD. This "Perspectives paper" will review current research involving microRNA (miRNA)-mediated, messenger RNA (mRNA)-targeted gene expression pathways in sporadic AD and address the potential implementation of evolving anti-microRNA (AM) strategies in the amelioration and clinical management of AD. This novel-therapeutic approach: (i) incorporates a system involving the restoration of multiple miRNA-regulated mRNA-targets via the use of selectively-stabilized AM species; and (ii) that via implementation of synthetic AMs, the abundance of only relatively small-families of miRNAs need be modulated or neutralized to re-establish neural-homeostasis in the AD-affected brain. In doing so, these strategic approaches will jointly and interactively address multiple AD-associated processes such as the disruption of synaptic communication, defects in amyloid peptide clearance and amyloidogenesis, tau pathology, deficits in neurotrophic support, alterations in the innate immune response, and the proliferation of neuroinflammatory signaling.


Subject(s)
Alzheimer Disease/genetics , Alzheimer Disease/pathology , Brain/pathology , Gene Targeting/methods , MicroRNAs/antagonists & inhibitors , MicroRNAs/genetics , Animals , Gene Targeting/trends , Genetic Vectors/administration & dosage , Genetic Vectors/genetics , Humans , Nervous System Diseases/genetics , Nervous System Diseases/pathology , Nervous System Diseases/therapy
16.
Sheng Wu Gong Cheng Xue Bao ; 35(5): 784-794, 2019 May 25.
Article in Chinese | MEDLINE | ID: mdl-31222997

ABSTRACT

The establishment and development of gene knockout mice have provided powerful support for the study of gene function and the treatment of human diseases. Gene targeting and gene trap are two techniques for generating gene knockout mice from embryonic stem cells. Gene targeting replaces endogenous knockout gene by homologous recombination. There are two ways to knock out target genes: promoter trap and polyA trap. In recent years, many new gene knockout techniques have been developed, including Cre/loxP system, CRISP/Cas9 system, latest ZFN technology and TALEN technology. This article focuses on the several new knockout mouse techniques.


Subject(s)
Gene Knockout Techniques , Mice, Knockout , Animals , Disease Models, Animal , Embryonic Stem Cells , Gene Knockout Techniques/trends , Gene Targeting/trends , Homologous Recombination , Humans , Mice
18.
Semin Perinatol ; 42(8): 525-530, 2018 Dec.
Article in English | MEDLINE | ID: mdl-30415764

ABSTRACT

CRISPR-based therapeutics have the potential to revolutionize the treatment of hereditary diseases, but current efforts to translate research to the bedside face significant technical, regulatory, and ethical hurdles. In this article, we discuss an underappreciated application of CRISPR: diagnostic testing, and argue that: (1) CRISPR diagnostics are poised to disrupt diagnostic practices including perinatal screening and (2) since CRISPR diagnostics pose minimal technical, regulatory and ethical hurdles (unlike CRISPR therapeutic uses) they are likely to be clinically relevant before CRISPR-based therapies, and thus warrant medical community's attention.


Subject(s)
CRISPR-Cas Systems , Gene Editing/methods , Gene Targeting/methods , Molecular Diagnostic Techniques/methods , Perinatology/methods , Secondary Prevention/methods , Gene Editing/trends , Gene Targeting/trends , Humans , Molecular Diagnostic Techniques/trends , Perinatology/trends , Point-of-Care Systems
19.
Curr Pharm Biotechnol ; 19(8): 604-610, 2018.
Article in English | MEDLINE | ID: mdl-29804529

ABSTRACT

BACKGROUND: RNA is increasingly recognized as a powerful molecule that can be used to control gene expression. Sophisticated, well-engineered RNA-based regulators are being developed as oligotherapeutics. METHODS: In particular, small activating RNAs (saRNAs) are promising therapeutic options for targeting human diseases. Numerous saRNAs targeting multiple cancers have been developed in preclinical models. One saRNA targeting C/EBPα is currently undergoing clinical trials in liver cancer. RESULTS AND CONCLUSION: In this review, we describe the current working model of the intracellular mechanism of saRNA, discuss the recent progress of saRNA therapeutics in preclinical and clinical trials, and current advances in targeted delivery using aptamers in detail.


Subject(s)
Gene Targeting/methods , Neoplasms/genetics , Neoplasms/therapy , RNA, Small Interfering/administration & dosage , RNA, Small Interfering/genetics , Clinical Trials as Topic/methods , Gene Expression , Gene Targeting/trends , Genetic Therapy/methods , Genetic Therapy/trends , Humans
20.
Eur Rev Med Pharmacol Sci ; 22(4): 1044-1050, 2018 02.
Article in English | MEDLINE | ID: mdl-29509253

ABSTRACT

OBJECTIVE: Neuroma is the most common intracranial tumor. The mechanism of miRNA in glioma has gradually been understood. The purpose of this study was to investigate the role of MicroRNA-129-3p (miR-129-3p) in the pathogenesis of glioblastoma (GBM). PATIENTS AND METHODS: Differential expression of miR-129-3p in samples was analyzed by bioinformatics. PCR was used to detect the expression of miR-129-3p in samples. CCK8 assay was used to detect the cell viability. Transfection of mimic and inhibitor altered the expression of miR-129-3p, and the biological function of miRNA was explored. Luciferase reporter gene was used to detect target genes of miRNA. E2F5 expression was inhibited by transfection of small interfering RNAs. Western blotting was used to detect protein expressions of cells. RESULTS: miR-129-3p was low-expressed in the tissue samples. By transfecting mimic and the inhibitor, we found that increasing the expression of miR-129-3p can inhibit the cell viability. In contrast, inhibition of miR-129-3p promoted cell growth. Luciferase reporter gene and Western blot results suggested that E2F5 can be used as the target gene of miR-129-3p. Knockdown the target gene of the miR-129-3p, E2F5, also inhibited proliferation of glioblastoma. CONCLUSIONS: miR-129-3p can inhibit the growth of glioblastoma by down-regulating the expression of E2F5. miR-129-3p can be a new target for the treatment of glioblastoma. Our research provides new ideas for the target therapy of glioma.


Subject(s)
Brain Neoplasms/metabolism , Cell Proliferation/physiology , E2F5 Transcription Factor/biosynthesis , Glioblastoma/metabolism , MicroRNAs/biosynthesis , Brain Neoplasms/genetics , Brain Neoplasms/pathology , Cell Line, Tumor , Cell Survival/physiology , Drug Delivery Systems/trends , E2F5 Transcription Factor/antagonists & inhibitors , E2F5 Transcription Factor/genetics , Gene Targeting/trends , Glioblastoma/genetics , Glioblastoma/pathology , Humans , MicroRNAs/genetics
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