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1.
PLoS One ; 16(11): e0260468, 2021.
Article in English | MEDLINE | ID: mdl-34843573

ABSTRACT

Centella asiatica is rich in medical and cosmetic properties. While physiological responses of C. asiatica to light have been widely reported, the knowledge of the effects of light on its gene expression is sparse. In this study, we used RNA sequencing (RNA-seq) to investigate the expression of the C. asiatica genes in response to monochromatic red and blue light. Most of the differentially expressed genes (DEGs) under blue light were up-regulated but those under red light were down-regulated. The DEGs encoded for CRY-DASH and UVR3 were among up-regulated genes that play significant roles in responses under blue light. The DEGs involved in the response to photosystem II photodamages and in the biosynthesis of photoprotective xanthophylls were also up-regulated. The expression of flavonoid biosynthetic DEGs under blue light was up-regulated but that under red light was down-regulated. Correspondingly, total flavonoid content under blue light was higher than that under red light. The ABI5, MYB4, and HYH transcription factors appeared as hub nodes in the protein-protein interaction network of the DEGs under blue light while ERF38 was a hub node among the DEGs under red light. In summary, stress-responsive genes were predominantly up-regulated under blue light to respond to stresses that could be induced under high energy light. The information obtained from this study can be useful to better understand the responses of C. asiatica to different light qualities.


Subject(s)
Centella/genetics , Gene Expression Regulation, Plant/radiation effects , Transcriptome/radiation effects , Centella/radiation effects , Genes, Plant/radiation effects , Light , Stress, Physiological/radiation effects
2.
Int J Mol Sci ; 22(9)2021 Apr 29.
Article in English | MEDLINE | ID: mdl-33946760

ABSTRACT

Fagopyrum tataricum 'Hokkai T10' is a buckwheat cultivar capable of producing large amounts of phenolic compounds, including flavonoids (anthocyanins), phenolic acids, and catechin, which have antioxidant, anticancer, and anti-inflammatory properties. In the present study, we revealed that the maize transcription factor Lc increased the accumulation of phenolic compounds, including sinapic acid, 4-hydroxybenzonate, t-cinnamic acid, and rutin, in Hokkai T10 hairy roots cultured under long-photoperiod (16 h light and 8 h dark) conditions. The transcription factor upregulated phenylpropanoid and flavonoid biosynthesis pathway genes, yielding total phenolic contents reaching 27.0 ± 3.30 mg g-1 dry weight, 163% greater than the total flavonoid content produced by a GUS-overexpressing line (control). In contrast, when cultured under continuous darkness, the phenolic accumulation was not significantly different between the ZmLC-overexpressing hairy roots and the control. These findings suggest that the transcription factor (ZmLC) activity may be light-responsive in the ZmLC-overexpressing hairy roots of F. tataricum, triggering activation of the phenylpropanoid and flavonoid biosynthesis pathways. Further studies are required on the optimization of light intensity in ZmLC-overexpressing hairy roots of F. tataricum to enhance the production of phenolic compounds.


Subject(s)
Fagopyrum/metabolism , Fagopyrum/radiation effects , Phenols/metabolism , Biosynthetic Pathways/genetics , Biosynthetic Pathways/radiation effects , Darkness , Fagopyrum/genetics , Flavonoids/biosynthesis , Gene Expression Regulation, Plant/radiation effects , Genes, Plant/radiation effects , Light , Plant Proteins/genetics , Plant Proteins/metabolism , Plant Roots/genetics , Plant Roots/metabolism , Plants, Genetically Modified , Transcription Factors/genetics , Transcription Factors/metabolism , Up-Regulation/radiation effects
3.
Int J Mol Sci ; 22(5)2021 Mar 04.
Article in English | MEDLINE | ID: mdl-33806362

ABSTRACT

Date palm (Phoenix dactylifera) is one of the most widespread fruit crop species and can tolerate drastic environmental conditions that may not be suitable for other fruit species. Excess UV-B stress is one of the greatest concerns for date palm trees and can cause genotoxic effects. Date palm responds to UV-B irradiation through increased DEG expression levels and elaborates upon regulatory metabolic mechanisms that assist the plants in adjusting to this exertion. Sixty-day-old Khalas date palm seedlings (first true-leaf stage) were treated with UV-B (wavelength, 253.7 nm; intensity, 75 µW cm-2 for 72 h (16 h of UV light and 8 h of darkness). Transcriptome analysis revealed 10,249 and 12,426 genes whose expressions were upregulated and downregulated, respectively, compared to the genes in the control. Furthermore, the differentially expressed genes included transcription factor-encoding genes and chloroplast- and photosystem-related genes. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to detect metabolite variations. Fifty metabolites, including amino acids and flavonoids, showed changes in levels after UV-B excess. Amino acid metabolism was changed by UV-B irradiation, and some amino acids interacted with precursors of different pathways that were used to synthesize secondary metabolites, i.e., flavonoids and phenylpropanoids. The metabolite content response to UV-B irradiation according to hierarchical clustering analysis showed changes in amino acids and flavonoids compared with those of the control. Amino acids might increase the function of scavengers of reactive oxygen species by synthesizing flavonoids that increase in response to UV-B treatment. This study enriches the annotated date palm unigene sequences and enhances the understanding of the mechanisms underlying UV-B stress through genetic manipulation. Moreover, this study provides a sequence resource for genetic, genomic and metabolic studies of date palm.


Subject(s)
Phoeniceae/metabolism , Phoeniceae/radiation effects , Ultraviolet Rays/adverse effects , Gene Expression Regulation, Plant/radiation effects , Genes, Chloroplast/radiation effects , Genes, Plant/radiation effects , Metabolic Networks and Pathways/genetics , Metabolic Networks and Pathways/radiation effects , Molecular Sequence Annotation , Oxidative Phosphorylation/radiation effects , Phoeniceae/genetics , Photosynthesis/radiation effects , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Leaves/radiation effects , Plant Proteins/genetics , RNA-Seq , Stress, Physiological/radiation effects , Transcription Factors/genetics , Transcriptome/radiation effects
4.
Sci China Life Sci ; 63(7): 1037-1052, 2020 Jul.
Article in English | MEDLINE | ID: mdl-32112268

ABSTRACT

Although natural variations in rice flavonoids exist, and biochemical characterization of a few flavonoid glycosyltransferases has been reported, few studies focused on natural variations in tricin-lignan-glycosides and their underlying genetic basis. In this study, we carried out metabolic profiling of tricin-lignan-glycosides and identified a major quantitative gene annotated as a UDP-dependent glycosyltransferase OsUGT706C2 by metabolite-based genome-wide association analysis. The putative flavonoid glycosyltransferase OsUGT706C2 was characterized as a flavonoid 7-O-glycosyltransferas in vitro and in vivo. Although the in vitro enzyme activity of OsUGT706C2 was similar to that of OsUGT706D1, the expression pattern and induced expression profile of OsUGT706C2 were very different from those of OsUGT706D1. Besides, OsUGT706C2 was specifically induced by UV-B. Constitutive expression of OsUGT706C2 in rice may modulate phenylpropanoid metabolism at both the transcript and metabolite levels. Furthermore, overexpressing OsUGT706C2 can enhance UV-B tolerance by promoting ROS scavenging in rice. Our findings might make it possible to use the glycosyltransferase OsUGT706C2 for crop improvement with respect to UV-B adaptation and/or flavonoid accumulation, which may contribute to stable yield.


Subject(s)
Flavonoids/metabolism , Glycosyltransferases/genetics , Glycosyltransferases/radiation effects , Oryza/genetics , Plants, Genetically Modified/metabolism , Plants, Genetically Modified/radiation effects , Adaptation, Physiological , Biosynthetic Pathways , Gene Expression Regulation, Plant/radiation effects , Genes, Plant/radiation effects , Genome-Wide Association Study , Glycosides/metabolism , Lignans/metabolism , Metabolome/radiation effects , Mutation
5.
Int J Radiat Biol ; 96(2): 267-275, 2020 02.
Article in English | MEDLINE | ID: mdl-31692404

ABSTRACT

Purpose: Heavy-ion beams and γ-rays are popular physical mutagenesis to generate mutations in higher plants. It has been found that they show different mutation frequencies and spectrums of phenotype induction, however, the characteristics of heavy-ion beams on genetic polymorphism have not been clarified by comparing with γ-rays.Materials and methods: In the present study, seeds of Arabidopsis thaliana were exposed to carbon-ion beams (with linear energy transfer (LET) of 50 keV/µm) and γ-rays (with average LET of 0.2 keV/µm) irradiation. By using inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) analysis, the genetic polymorphism of both M1 and M3 plants were investigated, respectively.Results: Carbon-ion beams induced relatively higher polymorphism rate in both M1 and M3 generation than γ-rays: the polymorphism rates of M1 plants derived from carbon-ion beams irradiation are 12.87% (ISSR-C) and 9.01% (RAPD-C), while are 7.67% (ISSR-γ) and 1.45% (RAPD-γ) of plants derived from γ-rays. In M3 generation, the polymorphism rates of ISSR-C, RAPD-C, ISSR-γ, and RAPD-γ are 17.64%, 22.79%, 12.10%, and 2.82%, respectively.Conclusions: In summary, the exposure to carbon-ion beams and γ-rays lead to the change of genomic DNA of A. thaliana, which could be tested in M1 plants and M3 plants by ISSR and RAPD technology. So, both carbon-ion beams and γ-rays can induce variations of genetic polymorphisms in M1 plants and M3 plants. The genetic polymorphisms of M1 plants and M3 plants induced by carbon-ion beams are higher than γ-rays, indicating that heavy-ion beams irradiations mutation breeding is more advantageous than conventional ionizing radiations. Average molecular polymorphism of M1 plants is lower than M3 mutants, by nearly 4.77% (ISSR-C), 13.78% (RAPD-C), 4.43% (ISSR-γ), and 1.37% (RAPD-γ). We hope our study will provide basic information for understanding the effects of carbon-ion beams and γ-rays for plant mutation breeding.


Subject(s)
Arabidopsis/genetics , Arabidopsis/radiation effects , Carbon , Gamma Rays , Heavy Ions , Polymorphism, Genetic , Seeds/radiation effects , DNA, Plant/radiation effects , Genes, Plant/radiation effects , Genome, Plant , Linear Energy Transfer , Mutagenesis , Mutation , Mutation Rate , Phenotype , Radiation, Ionizing , Random Amplified Polymorphic DNA Technique
6.
Environ Pollut ; 250: 618-626, 2019 Jul.
Article in English | MEDLINE | ID: mdl-31035144

ABSTRACT

Radioactive contamination of the natural areas is one of the most long-lasting anthropogenic impacts on the environment. Scots pine (Pinus sylvestris L.) is a promising organism for radiation-related research because of its high radiosensitivity, but the genome size of Pinacea species has imposed obstacles for high-throughput studies so far. In this work, we conducted the analysis of the de novo assembled transcriptome of Scots pine populations growing in the Chernobyl-affected zone, which is still today contaminated with radionuclides because of the accident at the nuclear power plant in 1986. The transcriptome profiles indicate a clear pattern of adaptive stress response, which seems to be dose-dependent. The transcriptional response indicates a continuous modulation of the cellular redox system, enhanced expression of chaperones and histones, along with the control of ions balance. Interestingly, the activity of transposable element families is inversely correlated to the exposure levels to radiation. These adaptive responses, which are triggered by radiation doses 30 times lower than the one accepted as a safe for biota species by international regulations, suggest that the environmental management in radiation protection should be reviewed.


Subject(s)
Chernobyl Nuclear Accident , Pinus sylvestris/radiation effects , Radiation Exposure , Gene Expression Profiling , Genes, Plant/radiation effects , Pinus sylvestris/genetics , Pinus sylvestris/metabolism , Radiation Monitoring
7.
J Plant Physiol ; 232: 23-26, 2019 Jan.
Article in English | MEDLINE | ID: mdl-30530200

ABSTRACT

Plant endogenous clock consists of self-sustained interlocked transcriptional/translational feedback loops whose oscillation regulates many circadian processes, including gene expression. Its free running rhythm can be entrained by external cues, which can influence all clock parameters. Among external cues, the geomagnetic field (GMF) has been demonstrated to influence plant growth and development. We evaluated the quantitative expression (qRT-PCR) of three clock genes (LHY, GI and PRR7) in time-course experiments under either continuous darkness (CD) or long days (LD) conditions in Arabidopsis thaliana seedlings exposed to GMF (∼40 µT) and Near Null Magnetic Field (NNMF; ∼40 nT) conditions. Under both LD and CD conditions, reduction of GMF to NNMF prompted a significant increase of the gene expression of LHY and PRR7, whereas an opposite trend was found for GI gene expression. Exposure of Arabidopsis to NNMF altered clock gene amplitude, regardless the presence of light, by reinforcing the morning loop. Our data are consistent with the existence of a plant magnetoreceptor that affects the Arabidopsis endogenous clock.


Subject(s)
Arabidopsis/radiation effects , Biological Clocks/radiation effects , Genes, Plant/radiation effects , Magnetic Fields , Arabidopsis/genetics , Arabidopsis/physiology , Arabidopsis Proteins/physiology , Arabidopsis Proteins/radiation effects , Biological Clocks/genetics , DNA-Binding Proteins/physiology , DNA-Binding Proteins/radiation effects , Gene Expression Profiling , Gene Expression Regulation, Plant/radiation effects , Genes, Plant/physiology , Light , Real-Time Polymerase Chain Reaction , Repressor Proteins/physiology , Repressor Proteins/radiation effects , Transcription Factors/physiology , Transcription Factors/radiation effects
8.
World J Microbiol Biotechnol ; 34(7): 96, 2018 Jun 18.
Article in English | MEDLINE | ID: mdl-29916185

ABSTRACT

The unicellular green microalga Haematococcus pluvialis has the highest content of the natural antioxidant, astaxanthin. Previously, it was determined that astaxanthin accumulation in H. pluvialis could be induced by blue-wavelength irradiation; however, the molecular mechanism remains unknown. The present study aimed to compare the transcriptome of H. pluvialis, with respect to astaxanthin biosynthesis, under the monochromatic red (660 nm) or blue (450 nm) light-emitting diode (LED) irradiation. Among a total of 165,372 transcripts, we identified 67,703 unigenes, of which 2245 and 171 were identified as differentially expressed genes (DEGs) in response to blue and red irradiation, respectively. Interestingly, expressional changes of blue light receptor cryptochromes were detected in response to blue and/or red LED irradiation in H. pluvialis, which may directly and indirectly regulate astaxanthin biosynthesis. In accordance with this observation, expression of the BKT and CHY genes, which are part of the downstream section of the astaxanthin biosynthetic pathway, was significantly upregulated by blue LED irradiation compared with their expression under control white irradiation. Contrastingly, they were downregulated by red LED irradiation. Our transcriptome study provided molecular insights that highlighted the different of responses of H. pluvialis to red and blue irradiation, especially for astaxanthin biosynthesis.


Subject(s)
Chlorophyta/genetics , Chlorophyta/metabolism , Chlorophyta/radiation effects , Gene Expression Regulation, Plant/radiation effects , Light , Transcriptome , Biosynthetic Pathways/genetics , Biosynthetic Pathways/radiation effects , Cell Division/radiation effects , Chlorophyta/growth & development , Cluster Analysis , Color , Gene Expression Profiling , Gene Ontology , Genes, Plant/genetics , Genes, Plant/radiation effects , Industrial Microbiology , Lighting , Reactive Oxygen Species/metabolism , Sequence Analysis, RNA , Up-Regulation , Xanthophylls/biosynthesis , Xanthophylls/genetics
9.
Plant Mol Biol ; 87(3): 303-15, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25537647

ABSTRACT

Thymidine kinase catalyzes the first step in the nucleotide salvage pathway by transferring a phosphate group to a thymidine molecule. In mammals thymidine kinase supplies deoxyribonucleotides for DNA replication and DNA repair, and the expression of the gene is tightly regulated during the cell cycle. Although this gene is phylogenetically conserved in many taxa, its physiological function in plants remains unknown. The genome of the model plant Arabidopsis thaliana has two thymidine kinase genes (AtTK1a and AtTK1b) and microarray data suggest they might have redundant roles. In this study we analyzed the TK1a function by evaluating its expression pattern during development and in response to genotoxic stress. We also studied its role in DNA repair by the characterization of a mutant that contained the T-DNA insertion in the promoter region of the TK1a gene. We found that TK1a is expressed in most tissues during plant development and it was differentially induced by ultraviolet-C radiation because TK1b expression was unaffected. In the mutant, the T-DNA insertion caused a 40 % rise in transcript levels and enzyme activity in Arabidopsis seedlings compared to wild-type plants. This elevation was enough to confer tolerance to ultraviolet-C irradiation in dark conditions, as determined by root growth, and meristem length and structure. TK1a overexpression also provided tolerance to genotoxins that induce double-strand break. Our results suggest that thymidine kinase contributes to several DNA repair pathways by providing deoxythymidine triphosphate that serve as precursors for DNA repair and to balance deoxyribonucleotides pools.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Arabidopsis/enzymology , Arabidopsis/genetics , Thymidine Kinase/genetics , Thymidine Kinase/metabolism , Arabidopsis/radiation effects , Base Sequence , DNA Damage , DNA, Bacterial/genetics , DNA, Plant/genetics , Gene Expression Regulation, Developmental/radiation effects , Gene Expression Regulation, Enzymologic/radiation effects , Gene Expression Regulation, Plant/radiation effects , Genes, Plant/radiation effects , Molecular Sequence Data , Mutagenesis, Insertional , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , Plants, Genetically Modified/radiation effects , Promoter Regions, Genetic , Seedlings/enzymology , Seedlings/genetics , Seedlings/radiation effects , Ultraviolet Rays/adverse effects
10.
Gene ; 541(2): 82-91, 2014 May 15.
Article in English | MEDLINE | ID: mdl-24631263

ABSTRACT

In order to better understand the biological systems that are affected in response to cosmic ray (CR), we conducted weighted gene co-expression network analysis using the module detection method. By using the Pearson's correlation coefficient (PCC) value, we evaluated complex gene-gene functional interactions between 680 CR-responsive probes from integrated microarray data sets, which included large-scale transcriptional profiling of 1000 microarray samples. These probes were divided into 6 distinct modules that contained 20 enriched gene ontology (GO) functions, such as oxidoreductase activity, hydrolase activity, and response to stimulus and stress. In particular, modules 1 and 2 commonly showed enriched annotation categories such as oxidoreductase activity, including enriched cis-regulatory elements known as ROS-specific regulators. These results suggest that the ROS-mediated irradiation response pathway is affected by CR in modules 1 and 2. We found 243 ionizing radiation (IR)-responsive probes that exhibited similarities in expression patterns in various irradiation microarray data sets. The expression patterns of 6 randomly selected IR-responsive genes were evaluated by quantitative reverse transcription polymerase chain reaction following treatment with CR, gamma rays (GR), and ion beam (IB); similar patterns were observed among these genes under these 3 treatments. Moreover, we constructed subnetworks of IR-responsive genes and evaluated the expression levels of their neighboring genes following GR treatment; similar patterns were observed among them. These results of network-based analyses might provide a clue to understanding the complex biological system related to the CR response in plants.


Subject(s)
Cosmic Radiation , Gene Expression Regulation, Plant , Gene Regulatory Networks , Oryza/genetics , Down-Regulation , Gamma Rays , Gene Expression Profiling , Gene Expression Regulation, Plant/radiation effects , Genes, Plant/genetics , Genes, Plant/radiation effects , Nucleotide Motifs , Oligonucleotide Array Sequence Analysis , Organ Specificity , Oryza/physiology , Oryza/radiation effects , Stress, Physiological , Transcriptome , Up-Regulation
11.
Planta ; 238(6): 1147-56, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24043576

ABSTRACT

The development of highly inducible promoters is critical for designing effective transformation systems for transgenic analyses. In this study, we investigated the promoter of the light-inducible protein gene (LIP) of the marine alga Dunaliella sp. LIPs are homologs of the early light-induced proteins (ELIPs) of Arabidopsis thaliana. DNA sequence analysis revealed that the LIP promoter contains several light-responsive motifs. Constructs containing progressive truncations of the LIP promoter fused with a Renilla luciferase gene were introduced into Chlamydomonas reinhardtii to identify the light-responsive region in the promoter. Transcription from the LIP promoter was stimulated by high light (HL) in a light intensity-dependent manner. In contrast, oxidative stress induced by chemicals had little effect on the LIP promoter, which implies that the LIP promoter is exclusively induced by high light. Truncation of the promoter to a -100 base pair (bp) region abrogated light inducibility, which suggests the presence of a negative cis-regulatory element upstream of the -100 bp fragment. The LIP promoter can be utilized in transgenic research to specifically select and propagate transgenic microalgae under high-light conditions.


Subject(s)
Chlamydomonas reinhardtii/genetics , Volvocida/genetics , 5' Untranslated Regions , Base Sequence , Chlamydomonas reinhardtii/radiation effects , Cloning, Molecular , DNA, Plant/genetics , Genes, Plant/radiation effects , Light , Plant Proteins/genetics , Promoter Regions, Genetic/radiation effects , Transformation, Genetic , Volvocida/radiation effects
12.
New Phytol ; 200(3): 861-874, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23845065

ABSTRACT

The effects of plant competition for light on the emission of plant volatile organic compounds (VOCs) were studied by investigating how different light qualities that occur in dense vegetation affect the emission of constitutive and methyl-jasmonate-induced VOCs. Arabidopsis thaliana Columbia (Col-0) plants and Pieris brassicae caterpillars were used as a biological system to study the effects of light quality manipulations on VOC emissions and attraction of herbivores. VOCs were analysed using gas chromatography-mass spectrometry and the effects of light quality, notably the red : far red light ratio (R : FR), on expression of genes associated with VOC production were studied using reverse transcriptase-quantitative PCR. The emissions of both constitutive and methyl-jasmonate-induced green leaf volatiles and terpenoids were partially suppressed under low R : FR and severe shading conditions. Accordingly, the VOC-based preference of neonates of the specialist lepidopteran herbivore P. brassicae was significantly affected by the R : FR ratio. We conclude that VOC-mediated interactions among plants and between plants and organisms at higher trophic levels probably depend on light alterations caused by nearby vegetation. Studies on plant-plant and plant-insect interactions through VOCs should take into account the light quality within dense stands when extrapolating to natural and agricultural field conditions.


Subject(s)
Acetates/metabolism , Arabidopsis/metabolism , Butterflies , Cyclopentanes/metabolism , Herbivory , Light , Oxylipins/metabolism , Plant Diseases , Volatile Organic Compounds/metabolism , Animals , Arabidopsis/genetics , Arabidopsis/radiation effects , Darkness , Gas Chromatography-Mass Spectrometry , Gene Expression/radiation effects , Genes, Plant/radiation effects , Plant Diseases/genetics , Plant Leaves/metabolism , Plant Leaves/radiation effects , Terpenes/metabolism
13.
Gene ; 520(2): 139-47, 2013 May 15.
Article in English | MEDLINE | ID: mdl-23454621

ABSTRACT

We used 2D-PAGE to isolate a light-induced protein (AL-A) that is expressed abundantly in light-growth alfalfa sprouts. The seven amino acids of the N-terminal region of the protein were identified, and we searched for the protein in GenBank using the BLAST program. The results of the homology analysis showed that the amino acid sequence of the isolated protein is most similar to one from a pea plastocyanin. To identify the protein, we amplified and sequenced the DNA fragment encoding AL-A from genomic alfalfa DNA. We found that the AL-A gene was highly homologous (90%) to the sequences from the pea plastocyanin via multiple alignments, and the deduced protein precursor was predicted to be chloroplast-specific via the ChloroP computer program. The protein was named alfalfa-plastocyanin (AL-P). It was characterized as being a light-inducible protein, and RT-PCR analysis showed that AL-P mRNA transcription only occurred in the leaves of the alfalfa plant and the alfalfa seedlings growth in lighted conditions. PCR was also used to amplify the DNA fragment encoding the AL-P promoter (AL-Pp) from genomic alfalfa DNA. PlantCARE analysis of the promoter sequence indicated that both a typical TATA box and a CAAT box were located in the promoter sequence, and some of the cis-elements that are responsible for light responsiveness were also identified within this promoter region. The AL-P gene promoter fused to the ß-glucuronidase (GUS) reporter gene has been examined for expression in transgenic alfalfa seedlings. Our findings have a potential application in plant genetic engineering; the AL-Pp may be used to drive the expression of heterologous genes in transgenic alfalfa plants.


Subject(s)
Gene Expression Regulation, Plant/radiation effects , Light , Medicago sativa/genetics , Plant Proteins/genetics , Plant Proteins/isolation & purification , Promoter Regions, Genetic/genetics , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Genes, Plant/radiation effects , Medicago sativa/chemistry , Medicago sativa/radiation effects , Molecular Sequence Data , Photosynthetic Reaction Center Complex Proteins/genetics , Photosynthetic Reaction Center Complex Proteins/isolation & purification , Photosynthetic Reaction Center Complex Proteins/metabolism , Plant Proteins/radiation effects , Seedlings/chemistry , Seedlings/genetics , Sequence Homology , Substrate Specificity/genetics
14.
Plant Sci ; 195: 106-19, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22921004

ABSTRACT

Plants exposed to ionising radiation (IR) have to face direct and indirect (oxidative stress) deleterious effects whose intensity depends on the dose applied and led to differential genome regulation. Transcriptomic analyses were conducted with CATMA microarray technology on Arabidopsis thaliana plantlets, 2 and 26h after exposure to the IR doses 10Gy and 40Gy. 10Gy treatment seemed to enhance antioxidative compound biosynthetic pathways whereas the 40Gy dose up-regulated ROS-scavenging enzyme genes. Transcriptomic data also highlighted a differential regulation of chloroplast constituent genes depending on the IR dose, 10Gy stimulating and 40Gy down-regulating. This probable 40Gy decrease of photosynthesis could help for the limitation of ROS production and may be coupled with programmed cell death (PCD)/senescence phenomena. Comparisons with previous transcriptomic studies on plants exposed to a 100Gy dose revealed 60 dose-dependent up-regulated genes, including notably cell cycle checkpoints to allow DNA repairing phenomena. Furthermore, the alteration of some cellular structure related gene expression corroborated a probable mitotic arrest after 40Gy. Finally, numerous heat-shock protein and chaperonin genes, known to protect proteins against stress-dependent dysfunction, were up-regulated after IR exposure.


Subject(s)
Antioxidants/metabolism , Arabidopsis Proteins/genetics , Arabidopsis/genetics , Gene Expression/radiation effects , Genes, Plant/radiation effects , Radiation, Ionizing , Transcriptome/genetics , Apoptosis/genetics , Apoptosis/radiation effects , Arabidopsis/metabolism , Arabidopsis/radiation effects , Arabidopsis Proteins/metabolism , Cell Cycle/genetics , Cell Cycle/radiation effects , Cellular Senescence/genetics , Cellular Senescence/radiation effects , Chaperonins/genetics , Chaperonins/metabolism , Chloroplasts/genetics , DNA Repair/genetics , DNA Repair/radiation effects , Enzymes/genetics , Enzymes/metabolism , Genome, Plant/radiation effects , Heat-Shock Proteins/genetics , Heat-Shock Proteins/metabolism , Metabolic Networks and Pathways/genetics , Metabolic Networks and Pathways/radiation effects , Mitosis/genetics , Mitosis/radiation effects , Oligonucleotide Array Sequence Analysis/methods , Oxidative Stress/genetics , Oxidative Stress/radiation effects , Photosynthesis/radiation effects , Reactive Oxygen Species/metabolism , Transcriptome/radiation effects
15.
Mutat Res ; 735(1-2): 19-31, 2012 Jul 01.
Article in English | MEDLINE | ID: mdl-22579628

ABSTRACT

Linear energy transfer (LET) is an important parameter to be considered in heavy-ion mutagenesis. However, in plants, no quantitative data are available on the molecular nature of the mutations induced with high-LET radiation above 101-124keVµm(-1). In this study, we irradiated dry seeds of Arabidopsis thaliana with Ar and C ions with an LET of 290keVµm(-1). We analyzed the DNA alterations caused by the higher-LET radiation. Mutants were identified from the M(2) pools. In total, 14 and 13 mutated genes, including bin2, egy1, gl1, gl2, hy1, hy3-5, ttg1, and var2, were identified in the plants derived from Ar- and C-ions irradiation, respectively. In the mutants from both irradiations, deletion was the most frequent type of mutation; 13 of the 14 mutated genes from the Ar ion-irradiated plants and 11 of the 13 mutated genes from the C ion-irradiated plants harbored deletions. Analysis of junction regions generated by the 2 types of irradiation suggested that alternative non-homologous end-joining was the predominant pathway of repair of break points. Among the deletions, the proportion of large deletions (>100bp) was about 54% for Ar-ion irradiation and about 64% for C-ion irradiation. Both current results and previously reported data revealed that the proportions of the large deletions induced by 290-keVµm(-1) radiations were higher than those of the large deletions induced by lower-LET radiations (6% for 22.5-30.0keVµm(-1) and 27% for 101-124keVµm(-1)). Therefore, the 290keVµm(-1) heavy-ion beams can effectively induce large deletions and will prove useful as novel mutagens for plant breeding and analysis of gene functions, particularly tandemly arrayed genes.


Subject(s)
Arabidopsis/radiation effects , Argon , Carbon , Genes, Plant/radiation effects , Heavy Ions/adverse effects , Linear Energy Transfer , Mutation/radiation effects , Base Sequence , DNA, Plant/genetics , Seeds/genetics , Seeds/radiation effects , Sequence Deletion
16.
Mutat Res ; 734(1-2): 56-61, 2012 Jun 01.
Article in English | MEDLINE | ID: mdl-22445891

ABSTRACT

The culm length of two semidwarf rice mutants (PKOS1, HyKOS1) obtained from low-energy N-ion beam bombardments of dehusked Thai jasmine rice (Oryza sativa L. cv. KDML 105) seeds showed 25.7% and 21.5% height reductions and one spindly rice mutant (TKOS4) showed 21.4% increase in comparison with that of the KDML 105 control. A cDNA-RAPD analysis identified differential gene expression in internode tissues of the rice mutants. Two genes identified from the cDNA-RAPD were OsSPY and 14-3-3, possibly associated with stem height variations of the semidwarf and spindly mutants, respectively. The OsSPY gene encoded the SPY protein which is considered to be a negative regulator of gibberellin (GA). On the other hand, the 14-3-3 encoded a signaling protein which can bind and prevent the RSG (repression of shoot growth) protein function as a transcriptional repressor of the kaurene oxidase (KO) gene in the GA biosynthetic pathway. Expression analysis of OsSPY, 14-3-3, RSG, KO, and SLR1 was confirmed in rice internode tissues during the reproductive stage of the plants by semi-quantitative RT-PCR technique. The expression analysis showed a clear increase of the levels of OsSPY transcripts in PKOS1 and HyKOS1 tissue samples compared to that of the KDML 105 and TKOS4 samples at the age of 50-60 days which were at the ages of internode elongation. The 14-3-3 expression had the highest increase in the TKOS4 samples compared to those in KDML 105, PKOS1 and HyKOS1 samples. The expression analysis of RSG and KO showed an increase in TKOS4 samples compared to that of the KDML 105 and that of the two semidwarf mutants. These results indicate that changes of OsSPY and 14-3-3 expression could affect internode elongation and cause the phenotypic changes of semidwarf and spindly rice mutants, respectively.


Subject(s)
14-3-3 Proteins/genetics , Genes, Plant , Oryza/growth & development , Oryza/genetics , Genes, Plant/radiation effects , Gibberellins/genetics , Heavy Ions , Mutation , Oryza/radiation effects , Plant Stems/genetics , Plants, Genetically Modified , Repressor Proteins/genetics
17.
Gene ; 486(1-2): 56-64, 2011 Oct 15.
Article in English | MEDLINE | ID: mdl-21787852

ABSTRACT

To obtain an overall view on gene expression during the early stage (24 h) of tomato fruit in response to postharvest UV-C irradiation (4 kJ/m(2)), we performed a microarray analysis by using Affymetrix Tomato Genechip. The results showed that 274 and 403 genes were up- or down-regulated, respectively, more than two folds in postharvest tomato fruit irradiated with UV-C as compared with that in control fruit. The up-regulated genes mainly involve in signal transduction, defense response and metabolism. Conversely, genes related to cell wall disassembly, photosynthesis and lipid metabolism were generally down-regulated. These results opened ways to probe into the molecular mechanisms of the effects of postharvest UV-C irradiation on increased disease resistance, delayed softening, better quality maintenance and prolonged postharvest life in tomato fruit.


Subject(s)
Food Irradiation , Genes, Plant/radiation effects , Solanum lycopersicum/genetics , Solanum lycopersicum/radiation effects , Cell Wall/genetics , DNA Primers/genetics , DNA, Plant/genetics , Ethylenes/biosynthesis , Fruit/genetics , Fruit/metabolism , Fruit/radiation effects , Gene Expression/radiation effects , Gene Expression Profiling , Lipid Metabolism/genetics , Solanum lycopersicum/metabolism , Oligonucleotide Array Sequence Analysis , Polymerase Chain Reaction , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Plant/genetics , RNA, Plant/metabolism , Ultraviolet Rays
18.
Indian J Exp Biol ; 49(6): 429-37, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21702222

ABSTRACT

In the present paper, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) was used to examine and identify differentially expressed genes in Capsicum annuum exposed to UV-B irradiation. Around 4000 transcript derived fragments (TDFs) were visualized and in total 183 TDFs were isolated, sequenced and analyzed by Blast 2 go. Among these TDFs, 84 of them showed homology to known genes. There were 43 TDFs showing up-regulated expression, 24 TDFs showing down-regulated expression and 29 TDFs showing both up-regulated and down-regulated expression, respectively. Some of these TDFs were found to be in response/related to UV-B stress, including carbonic anhydrase, calcium-dependent protein, thionin-like protein, bzip protein and so on. In particular, chlorophyll a/b binding protein (Capcab) responding to UV-B stress was cloned. It was concluded that Capcab could play a protective role in plant anti-UV-B and maintaining photosynthetic rate under UV-B stress.


Subject(s)
Capsicum/genetics , Capsicum/radiation effects , Base Sequence , DNA, Plant/genetics , Gene Expression/radiation effects , Genes, Plant/radiation effects , Plant Proteins/genetics , Radiation Tolerance/genetics , Stress, Physiological/genetics , Stress, Physiological/radiation effects , Ultraviolet Rays
19.
Biochem J ; 415(1): 11-9, 2008 Oct 01.
Article in English | MEDLINE | ID: mdl-18778247

ABSTRACT

In higher plants, light is crucial for regulation of nitrate uptake, translocation and assimilation into organic compounds. Part of this metabolism is tightly coupled to photosynthesis because the enzymes involved, nitrite reductase and glutamate synthase, are localized to the chloroplasts and receive reducing power from photosynthetic electron transport. However, important enzymes in nitrate acquisition and reduction are localized to cellular compartments other than chloroplasts and are also up-regulated by light, i.e. transporters in cell and organellar membranes and nitrate reductase in the cytosol. This review describes the different light-dependent signalling cascades regulating nitrate metabolism at the transcriptional as well as post-transcriptional level, and how reactions in different compartments of the cell are co-ordinated. Essential players in this network are phytochrome and HY5 (long hypocotyls 5)/HYH (HY5 homologue)-dependent signalling pathways, the energy-related AMPK (AMP-activated protein kinase) protein kinase homologue SNRK1 (sucrose non-fermenting kinase 1-related kinase), chloroplastic thioredoxins and the prokaryotically originated PII protein. A complex light-dependent network of regulation emerges, which appears to be necessary for optimal nitrogen assimilation and for avoiding the accumulation of toxic intermediates and side products, such as nitrite and reactive oxygen compounds.


Subject(s)
Nitrates/metabolism , Phytochrome/physiology , Plant Physiological Phenomena/radiation effects , Signal Transduction/radiation effects , AMP-Activated Protein Kinases , Arabidopsis/physiology , Arabidopsis Proteins/physiology , Basic-Leucine Zipper Transcription Factors/physiology , Carrier Proteins/physiology , Chloroplast Thioredoxins/metabolism , Chloroplasts/metabolism , Circadian Rhythm , DNA-Binding Proteins , Dicarboxylic Acid Transporters/physiology , Genes, Plant/radiation effects , Glutamate-Ammonia Ligase/physiology , Light , Multienzyme Complexes/physiology , Nitrate Reductase/physiology , Nitrite Reductases/physiology , Nitrites/metabolism , Nuclear Proteins/physiology , PII Nitrogen Regulatory Proteins/physiology , Phosphoprotein Phosphatases/physiology , Protein Serine-Threonine Kinases/physiology
20.
BMC Syst Biol ; 2: 16, 2008 Feb 04.
Article in English | MEDLINE | ID: mdl-18248680

ABSTRACT

BACKGROUND: One of the eminent opportunities afforded by modern genomic technologies is the potential to provide a mechanistic understanding of the processes by which genetic change translates to phenotypic variation and the resultant appearance of distinct physiological traits. Indeed much progress has been made in this area, particularly in biomedicine where functional genomic information can be used to determine the physiological state (e.g., diagnosis) and predict phenotypic outcome (e.g., patient survival). Ecology currently lacks an analogous approach where genomic information can be used to diagnose the presence of a given physiological state (e.g., stress response) and then predict likely phenotypic outcomes (e.g., stress duration and tolerance, fitness). RESULTS: Here, we demonstrate that a compendium of genomic signatures can be used to classify the plant abiotic stress phenotype in Arabidopsis according to the architecture of the transcriptome, and then be linked with gene coexpression network analysis to determine the underlying genes governing the phenotypic response. Using this approach, we confirm the existence of known stress responsive pathways and marker genes, report a common abiotic stress responsive transcriptome and relate phenotypic classification to stress duration. CONCLUSION: Linking genomic signatures to gene coexpression analysis provides a unique method of relating an observed plant phenotype to changes in gene expression that underlie that phenotype. Such information is critical to current and future investigations in plant biology and, in particular, to evolutionary ecology, where a mechanistic understanding of adaptive physiological responses to abiotic stress can provide researchers with a tool of great predictive value in understanding species and population level adaptation to climate change.


Subject(s)
Arabidopsis/genetics , Environment , Gene Expression Profiling , Gene Expression Regulation, Plant , Genes, Plant/genetics , Arabidopsis/drug effects , Disasters , Gene Expression Regulation, Plant/drug effects , Gene Expression Regulation, Plant/radiation effects , Gene Regulatory Networks/drug effects , Gene Regulatory Networks/radiation effects , Genes, Plant/drug effects , Genes, Plant/radiation effects , Osmosis , Phenotype , Salts/pharmacology , Temperature , Ultraviolet Rays
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