ABSTRACT
The succession of some markers on the chromosomes of Actinomadura carminata was shown with the method of successive mutagenesis based on the preferable effect of the mutagen on DNA in the site of replication. The spore germination was synchronized by selection of the spores of the definite size and maintenance at a temperature of 0 degrees. The periods of formation of the highest numbers of morphological and auxotrophic mutants were compared with the time of DNA replication.
Subject(s)
Actinomycetales/radiation effects , Antibiotics, Antineoplastic/biosynthesis , Carubicin/biosynthesis , Mutation/radiation effects , Ultraviolet Rays , Cell Survival/radiation effects , Culture Media , Genetic Variation/drug effects , Genetics, Microbial/radiation effects , Molecular Biology/radiation effects , Spores, Bacterial/radiation effects , Time FactorsSubject(s)
DNA, Bacterial/radiation effects , Escherichia coli/radiation effects , Ultraviolet Rays , Bacillus subtilis/radiation effects , Chromosomes, Bacterial/radiation effects , DNA Nucleotidyltransferases/metabolism , DNA Repair , Deoxyribodipyrimidine Photo-Lyase/metabolism , Exonucleases/metabolism , Genetics, Microbial/radiation effects , Polynucleotide Ligases/metabolism , Recombination, GeneticSubject(s)
DNA/radiation effects , Thymine/radiation effects , Uracil/radiation effects , Bacillus subtilis/radiation effects , Bacteriophages/radiation effects , Bromouracil/radiation effects , DNA, Bacterial/radiation effects , DNA, Viral/radiation effects , Dose-Response Relationship, Radiation , Genetics, Microbial/radiation effects , Transformation, Genetic/radiation effects , Ultraviolet RaysSubject(s)
Genetics, Microbial , Radiation Effects , Actinomyces/drug effects , Actinomyces/metabolism , Actinomyces/radiation effects , Anti-Bacterial Agents/metabolism , Ethylenes/pharmacology , Genetics, Microbial/drug effects , Genetics, Microbial/radiation effects , Genotype , Hybridization, Genetic , Imines/pharmacology , Mutagens/pharmacology , Mutation/drug effects , Mutation/radiation effects , Penicillins/metabolism , Penicillium/metabolism , Recombination, Genetic , Sulfuric Acids/pharmacologySubject(s)
Chlortetracycline/pharmacology , Genetics, Microbial/drug effects , Mutation/drug effects , Nitrites/pharmacology , Radiation Effects , Streptomyces/drug effects , Streptomycin/pharmacology , Ultraviolet Rays , Chlortetracycline/biosynthesis , Chlortetracycline/metabolism , Chromatography, Paper , Culture Techniques , Demeclocycline/biosynthesis , Genetics, Microbial/radiation effects , Mutation/radiation effects , Streptomyces/radiation effects , Tetracycline/biosynthesisABSTRACT
Ultraviolet light (UV) impaired the capacity of L cells to support growth of encephalomyocarditis virus. The loss of capacity was partially restored by high multiplicity of infection (MOI). This phenomenon was not due to an increased probability of an infectious virus particle reaching a site of replication undamaged by UV, since UV-inactivated virus at high MOI induced restoration of the capacity to support multiplication of nonirradiated virus adsorbed at low MOI. Multiplicity reactivation of UV-irradiated virus did not play a role in this phenomenon since restoration of capacity took place without multiplication of the UV-irradiated restoring virus. The evidence indicates that restoration of capacity was not due to viral interactions involving genetic exchange. The ability to restore capacity was a property more radioresistant than infectivity, suggesting that the former is a function only of part of the viral genome.
