ABSTRACT
An immunoperoxidase technique was used to compare the number of CD1a+ and factor XIIIa+ dendritic cells (DCs), and CD68+ Macrophages (M) in 30 gingival samples from subjects with clinically healthy periodontitium (HP) and 10 samples from subjects with drug-induced gingival enlargement (DIGE). Fewer CD1a+ and factor XIIIa+ DCs were found in areas with inflammatory infiltration (II) of the lamina propria (LP) in the group with immunosuppressed DIGE (IDIGE) compared to the group with HP. In the sulcular and junctional/pocket epithelia, the number of CD1a+ DCs was decreased in the group with IDIGE (p<0.05). There was a tendency toward a reduced number of CD1a+ DCs and CD68+ M in areas without inflammatory infiltrate of the LP in the group with IDIGE. The alterations in the number of antigen-presenting cells (APCs) may be the reason for the decreased periodontal inflammation and breakdown clinically observed in subjects who are immunosuppressed.
Subject(s)
Antigen-Presenting Cells/pathology , Gingival Hypertrophy/chemically induced , Immunosuppressive Agents/adverse effects , Adult , Antigen-Presenting Cells/immunology , Antigens, CD/analysis , Antigens, CD1/analysis , Antigens, Differentiation, Myelomonocytic/analysis , Dendritic Cells/immunology , Dendritic Cells/pathology , Epithelial Attachment/immunology , Epithelial Attachment/pathology , Epithelium/immunology , Epithelium/pathology , Factor XIIIa/analysis , Female , Gingival Crevicular Fluid/immunology , Gingival Hypertrophy/immunology , Humans , Immunoenzyme Techniques , Langerhans Cells/immunology , Langerhans Cells/pathology , Macrophages/immunology , Macrophages/pathology , Male , Middle Aged , Periodontal Pocket/immunology , Periodontal Pocket/pathology , Periodontium/immunology , Periodontium/pathologyABSTRACT
The aim of this study was to evaluate, clinically and histologically, the effect of Cyclosporin-A (CyA) on the progression of the periodontal breakdown in the domestic ferret, using the ligature induced periodontitis model. At the start of the experiment (day 0), silk ligatures were placed at the gingival margin level of experimental teeth. The contralateral teeth served as non-ligated control teeth. Clinical measurements included gingival index, probing pocket depth (PPD), probing attachment level (PAL) and gingival overgrowth (GO) and they were performed on days 0, 14 and 28. Cyclosporin-A, 10 mg/kg/d, was given subcutaneously from day 0 to 21, while in the last week there was a reduction of this dose to 5 mg/kg/d. Blood samples were taken on days 0, 14 and 28. On day 28 the animals were sacrificed. Histological sections were prepared for light microscopy. The histometric measurements performed were: (1) the distance between cemento-enamel junction and the alveolar bone crest and (2) loss of connective tissue attachment. The number of sections with root resorption areas was observed. Cell counts were taken in 4 different areas. The results showed, on days 14 and 28, a significant increase of the mean values of PPD and PAL at experimental teeth as compared to the control teeth. GO was present on experimental teeth on days 14 and 28. On day 28, the control teeth presented a very small increase in GO, mainly at the buccal sites of P4 and M1. The histometric results showed a significant loss of attachment and bone resorption in the experimental teeth. Root resorption was found in experimental teeth only. The predominant inflammatory cell in the 4 experimental areas was polymorphonuclears.(ABSTRACT TRUNCATED AT 250 WORDS)
