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1.
Molecules ; 24(14)2019 Jul 23.
Article in English | MEDLINE | ID: mdl-31340590

ABSTRACT

Honey maturity is an important factor in evaluating the quality of honey. We established a method for the identification of natural mature acacia honey with eighteen physicochemical parameters combined with chemometric analysis. The analysis of variance showed significant differences between mature and immature acacia honey in physicochemical parameters. The principal component analysis explained 82.64% of the variance among samples, and indicated that total phenolic content, total protein content, and total sugar (glucose, fructose, sucrose) were the major variables. The cluster analysis and orthogonal partial least squares-discriminant analysis demonstrated that samples were grouped in relation to the maturity coinciding with the results of the principal component analysis. Meanwhile, the 35 test samples were classified with 100% accuracy with the method of multi-physicochemical parameters combined with chemometric analysis. All the results presented above proved the possibility of identifying mature acacia honey and immature acacia honey according to the chemometric analysis based on the multi-physicochemical parameters.


Subject(s)
Acacia/chemistry , Food Quality , Honey/analysis , Pollen/chemistry , Analysis of Variance , Animals , Bees/physiology , China , Chromatography, High Pressure Liquid , Fructose/classification , Fructose/isolation & purification , Glucose/classification , Glucose/isolation & purification , Humans , Least-Squares Analysis , Phenols/classification , Phenols/isolation & purification , Principal Component Analysis , Sucrose/classification , Sucrose/isolation & purification
2.
Braz. j. pharm. sci ; 48(4): 601-607, Oct.-Dec. 2012. ilus, tab
Article in English | LILACS | ID: lil-665856

ABSTRACT

Aqueous extracts of Zingiber officinale rhizomes were studied to evaluate their antidiabetic effects on protein glycation and on the diffusion of glucose in vitro in the present study. Zingiber officinale rhizome aqueous extract were examined at concentrations of 5, 10, 20 and 40 g/L. The antidiabetic effects were found to be dose-dependent. Antidiabetic potential of Zingiber officinale was mainly through inhibition of the glucose diffusion and to a limited extent by reducing the glycation. However, further studies are needed to determine in vitro effects of therapeutic potential by restraining postprandial glucose absorptions and plasma protein glycations in diabetic subjects.


Extratos aquosos de rizomas Zingiber officinale foram estudados para avaliar os seus efeitos antidiabéticos em glicação de proteínas e sobre a difusão de glicose in vitro, no presente estudo. Extratos aquosos de Zingiber officinale foram examinados nas concentrações de 5, 10, 20 e 40 g extrato de planta/L. Os efeitos antidiabéticos observados eram dependentes da dose. O potencial antidiabético de Zingiber officinale se verificou, principalmente, através da inibição da difusão de glicose e, em menor extensão, através da redução da glicação. Estudos adicionais são necessários para elucidar se efeitos in vitro representam potencial terapêutico, restringindo a absorção de glicose pós-prandial e a glicação de proteínas plasmáticas em indivíduos diabéticos.


Subject(s)
In Vitro Techniques/classification , Zingiber officinale , Hypoglycemic Agents/antagonists & inhibitors , Pharmacognosy/classification , Diabetes Mellitus , Glucose/classification , Hyperglycemia
3.
Rev. Ciênc. Méd. Biol. (Impr.) ; 3(1): 60-68, jan.-jun. 2004. tab
Article in Portuguese | LILACS, BBO - Dentistry | ID: lil-481922

ABSTRACT

O objetivo do presente estudo foi verificar se o tratamento periodontal não cirúrgico exercia influência sobre os elementos celulares do sangue, perfil lipídico e glicose de pacientes com periodontite crônica. Dez pacientes (49,7 ± 8,8 anos) foram submetidos, previamente ao tratamento periodontal e, trinta dias após o mesmo, à coleta de 10ml de sangue periférico, através dos quais foram avaliados elementos celulares das séries branca e vermelha, número de plaquetas, VHS, lipidograma e glicose. Os parâmetros clínicos utilizados foram: Índice de Placa (IP) de Silness e L÷e (1964), Índice Gengival (IG) de Loe (1967), Sangramento à Sondagem (SS), Profundidade de Bolsa à Sondagem (PBS) e Nível de Inserção à Sondagem (NI). Trinta dias após a conclusão do tratamento periodontal não cirúrgico, foi realizada reavaliação e constatada melhora significativa (P<0,001) de todos os parâmetros clínicos supracitados. A hemoglobina (P=0,03) e a glicose (P=0,02) sofreram redução significativa, enquanto o número de hemácias (P=0,06) e o hematócrito (P=0,08), apesar de reduzidos, não sofreram mudanças estatisticamente significativas. O colesterol total e o LDL apresentaram aumento, porém não significativo, após o tratamento (P=0,09 e P=0,06, respectivamente), enquanto os demais parâmetros não sofreram alterações significativas (P>0,1). Através destes resultados, concluiu-se que o tratamento periodontal não cirúrgico foi bem sucedido no controle da periodontite crônica, e que, após o mesmo, a hemoglobina e a glicose sofreram redução estatisticamente significativa, as hemßcias e o hematócrito apresentaram uma tendência à redução e o colesterol total e o LDL, uma tendência ao aumento.


Subject(s)
Humans , Male , Female , Blood Cell Count , Blood Cells , Erythrocytes/classification , Glucose/classification , Leukocytes/classification , Lipids/classification , Periodontitis/blood
4.
Biosens Bioelectron ; 19(3): 177-83, 2003 Nov 30.
Article in English | MEDLINE | ID: mdl-14611752

ABSTRACT

The direct electrochemistry of glucose oxidase (GOD) adsorbed on a colloidal gold modified carbon paste electrode was investigated. The adsorbed GOD displayed a pair of redox peaks with a formal potential of -(449+/-1) mV in 0.1 M pH 5.0 phosphate buffer solution. The response showed a surface-controlled electrode process with an electron transfer rate constant of (38.9+/-5.3)/s determined in the scan rate range from 10 to 100 mV/s. GOD adsorbed on gold colloid nanoparticles maintained its bioactivity and stability. The immobilized GOD could electrocatalyze the reduction of dissolved oxygen and resulted in a great increase of the reduction peak current. Upon the addition of glucose, the reduction peak current decreased, which could be used for glucose detection with a high sensitivity (8.4 microA/mM), a linear range from 0.04 to 0.28 mM and a detection limit of 0.01 mM at a signal-to-noise ratio of 3sigma. The sensor could exclude the interference of commonly coexisted uric and ascorbic acid.


Subject(s)
Biosensing Techniques/instrumentation , Blood Glucose/analysis , Electrochemistry/instrumentation , Glucose Oxidase/chemistry , Glucose/analysis , Glucose/classification , Gold/chemistry , Graphite/chemistry , Biosensing Techniques/methods , Coated Materials, Biocompatible/chemical synthesis , Coated Materials, Biocompatible/chemistry , Colloids/chemistry , Electrochemistry/methods , Electrodes , Electron Transport , Enzymes, Immobilized/chemistry , Equipment Failure Analysis , Humans , Indicators and Reagents , Ointments , Reproducibility of Results , Sensitivity and Specificity
5.
In. Casasbuenas, Jaime; Chalem, Fernando, ed. Compendio de terapeutica. s.l, Acta Medica Colombiana, jul. 1988. p.123-37, tab.
Monography in Spanish | LILACS | ID: lil-117082
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