ABSTRACT
Enzyme/polymer/gold nanoparticle hybrids, called "nanozymes", were prepared and structurally analyzed by dynamic light scattering (DLS), ultraviolet-visible spectroscopy, and zeta-potential and transmission electron microscopy (TEM) measurements, which showed that the nanozyme particles were mainly composed of a single gold nanoparticle, on whose surface the enzyme and polymer were coimmobilized. This kind of structure resulted in the high dispersion stability of the nanozyme under various conditions, accompanied by improved thermal stability of the enzyme.
Subject(s)
Enzymes, Immobilized/chemistry , Glucose Dehydrogenases/chemistry , Glucose Dehydrogenases/metabolism , Gold/chemistry , Metal Nanoparticles/chemistry , Polymers/chemistry , Water/chemistry , Acinetobacter calcoaceticus/enzymology , Enzymes, Immobilized/metabolism , Enzymes, Immobilized/ultrastructure , Glucose Dehydrogenases/ultrastructure , Hydrogen-Ion Concentration , Kinetics , Metal Nanoparticles/ultrastructure , Microscopy, Electron, Transmission , Surface Plasmon ResonanceABSTRACT
Single crystals of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapour diffusion method and polyethylene glycol as a precipitant in the presence of NADP+ at pH 5.4. The crystals belong to the hexagonal space group P6122 or P6522, with unit cell dimensions a = b = 121.9 angstrom, c = 229.6 angstrom and with two molecules in the asymmetric unit.
