ABSTRACT
Glucose 6-phosphate dehydrogenase (G6PD) deficiency facilitates human coronavirus infection due to glutathione depletion. G6PD deficiency may especially predispose to hemolysis upon coronavirus disease-2019 (COVID-19) infection when employing pro-oxidant therapy. However, glutathione depletion is reversible by N-acetylcysteine (NAC) administration. We describe a severe case of COVID-19 infection in a G6PD-deficient patient treated with hydroxychloroquine who benefited from intravenous (IV) NAC beyond reversal of hemolysis. NAC blocked hemolysis and elevation of liver enzymes, C-reactive protein (CRP), and ferritin and allowed removal from respirator and veno-venous extracorporeal membrane oxygenator and full recovery of the G6PD-deficient patient. NAC was also administered to 9 additional respirator-dependent COVID-19-infected patients without G6PD deficiency. NAC elicited clinical improvement and markedly reduced CRP in all patients and ferritin in 9/10 patients. NAC mechanism of action may involve the blockade of viral infection and the ensuing cytokine storm that warrant follow-up confirmatory studies in the setting of controlled clinical trials.
Subject(s)
Acetylcysteine/therapeutic use , Antioxidants/therapeutic use , Betacoronavirus/pathogenicity , Coronavirus Infections/drug therapy , Cytokine Release Syndrome/drug therapy , Glucosephosphate Dehydrogenase Deficiency/drug therapy , Pneumonia, Viral/drug therapy , Adult , Antirheumatic Agents/therapeutic use , Biomarkers/blood , C-Reactive Protein/metabolism , COVID-19 , Coronavirus Infections/blood , Coronavirus Infections/complications , Coronavirus Infections/virology , Cytokine Release Syndrome/blood , Cytokine Release Syndrome/complications , Cytokine Release Syndrome/virology , Drug Administration Schedule , Ferritins/blood , Fibrin Fibrinogen Degradation Products/metabolism , Glucosephosphate Dehydrogenase Deficiency/blood , Glucosephosphate Dehydrogenase Deficiency/complications , Glucosephosphate Dehydrogenase Deficiency/virology , Humans , Hydroxychloroquine/therapeutic use , Inflammation/prevention & control , Male , Pandemics , Pneumonia, Viral/blood , Pneumonia, Viral/complications , Pneumonia, Viral/virology , SARS-CoV-2 , Treatment OutcomeABSTRACT
We report a case of intravascular hemolysis and methemoglobinemia, precipitated by severe acute respiratory syndrome coronavirus 2 infection, in a patient with undiagnosed glucose-6-phosphate dehydrogenase deficiency. Clinicians should be aware of this complication of coronavirus disease as a cause of error in pulse oximetry and a potential risk for drug-induced hemolysis.
Subject(s)
Betacoronavirus , Coronavirus Infections/complications , Glucosephosphate Dehydrogenase Deficiency/virology , Methemoglobinemia/virology , Pneumonia, Viral/complications , COVID-19 , Coronavirus Infections/blood , Coronavirus Infections/virology , Glucosephosphate Dehydrogenase Deficiency/blood , Humans , Male , Methemoglobinemia/blood , Middle Aged , Pandemics , Pneumonia, Viral/blood , Pneumonia, Viral/virology , SARS-CoV-2ABSTRACT
Variations in the cellular microenvironment affect the host's susceptibility to pathogens. Using glucose-6-phosphate dehydrogenase (G6PD)-deficient fibroblasts as a model, this study demonstrated that the cellular redox status affects infectivity as well as the outcome of enterovirus 71 (EV71) infection. Compared with their normal counterparts, G6PD-deficient cells supported EV71 replication more efficiently and showed greater cytopathic effect and loss of viability. Mechanistically, viral infection led to increased oxidative stress, as indicated by increased dichlorofluorescein fluorescence and a diminished ratio of glutathione (GSH) to its disulfide form (GSSG), with the effect being greater in G6PD-deficient cells. Exogenous expression of active G6PD in the deficient cells, which increased the intracellular GSH:GSSG ratio, suppressed the generation of viral progeny. Consistent with this, treatment with N-acetylcysteine offered resistance to EV71 propagation and a cytoprotective effect on the infected cells. These findings support the notion that G6PD status, and thus redox balance, is an important determinant of enteroviral infection.
Subject(s)
Enterovirus A, Human/pathogenicity , Enterovirus Infections/enzymology , Enterovirus Infections/etiology , Glucosephosphate Dehydrogenase/metabolism , Antioxidants/pharmacology , Base Sequence , Cells, Cultured , Cytopathogenic Effect, Viral , DNA, Viral/genetics , Enterovirus A, Human/genetics , Enterovirus A, Human/physiology , Enterovirus Infections/prevention & control , Enterovirus Infections/virology , Glucosephosphate Dehydrogenase Deficiency/complications , Glucosephosphate Dehydrogenase Deficiency/metabolism , Glucosephosphate Dehydrogenase Deficiency/virology , Glutathione , Glutathione Disulfide/metabolism , Humans , Oxidative Stress , Viral Plaque Assay , Virulence , Virus ReplicationABSTRACT
The host cellular environment is a key determinant of pathogen infectivity. Viral gene expression and viral particle production of glucose-6-phosphate dehydrogenase (G6PD)-deficient and G6PD-knockdown cells were much higher than their counterparts when human coronavirus (HCoV) 229E was applied at 0.1 multiplicity of infection. These phenomena were correlated with increased oxidant production. Accordingly, ectopic expression of G6PD in G6PD-deficient cells or addition of antioxidant (such as alpha-lipoic acid) to G6PD-knockdown cells attenuated the increased susceptibility to HCoV 229E infection. All experimental data indicated that oxidative stress in host cells is an important factor in HCoV 229E infectivity.
