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1.
Analyst ; 149(7): 1971-1975, 2024 Mar 25.
Article in English | MEDLINE | ID: mdl-38439614

ABSTRACT

Herein, we present toxicological assessments of carbon nanomaterials in HL-7702 cells, and it was found that reactive oxygen species (ROS) levels were elevated. Mass spectrometry results indicated that cysteine sulfhydryl of glutaredoxin-1 (GLRX1) was oxidized to sulfenic acids and sulfonic acids by excessive ROS, which broke the binding of GLRX1 to apoptosis signal-regulating kinase 1, causing the activation of the JNK/p38 signaling pathway and ultimately hepatocyte apoptosis. However, a lower level of ROS upregulated GLRX1 instead of sulfonation modification of its active sites. Highly expressed GLRX1 in turn enabled the removal of intracellular ROS, thereby exerting inconspicuous toxic effects on cells. Taken together, these findings emphasized that CNM-induced hepatotoxicity is attributable to oxidative modifications of GLRX1 arising from redox imbalance.


Subject(s)
Chemical and Drug Induced Liver Injury , Glutaredoxins , Humans , Reactive Oxygen Species/metabolism , Glutaredoxins/genetics , Glutaredoxins/metabolism , Glutaredoxins/pharmacology , Oxidation-Reduction , Apoptosis , Oxidative Stress
2.
Exp Eye Res ; 234: 109609, 2023 09.
Article in English | MEDLINE | ID: mdl-37541331

ABSTRACT

Glutaredoxin 2 (Grx2), a mitochondrial glutathione-dependent oxidoreductase, is crucial for maintaining redox homeostasis and cellular functions in the lens. The oxidative stress-induced epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) is related to posterior capsule opacification. In this study, we investigated the effects of Grx2 on oxidative stress-induced EMT in LECs during posterior capsule opacification. We found that Grx2 expression was substantially decreased during the EMT of LECs and in a mouse model of cataract surgery. Deletion of Grx2 aggravated the generation of reactive oxygen species, including those that are mitochondria-derived, and promoted the proliferation and EMT of the LECs. This was reversed by Grx2 overexpression. In vivo, proteomic liquid chromatography-mass spectrometry analysis showed that integrin-linked kinase (ILK) was significantly upregulated in the lens posterior capsule of a Grx2 knockout (KO) mouse model. Compared with that of the wild-type group, the expression of ILK and EMT markers was increased in the Grx2 KO group which was reversed in the Grx2 knock-in group. Inhibition of ILK partially blocked Grx2 knockdown-induced EMT and prevented the increased phosphorylation of Akt and GSK-3ß and the nuclear translocation of ß-catenin in the Grx2 KO group. Finally, inhibition of the Wnt/ß-catenin pathway partially blocked the Grx2 knockdown-induced EMT. In conclusion, we demonstrated that Grx2 protects LECs from oxidative stress-related EMT by regulating the ILK/Akt/GSK-3ß axis.


Subject(s)
Capsule Opacification , Lens, Crystalline , Animals , Mice , beta Catenin/metabolism , Capsule Opacification/metabolism , Epithelial Cells/metabolism , Epithelial-Mesenchymal Transition , Glutaredoxins/genetics , Glutaredoxins/metabolism , Glutaredoxins/pharmacology , Glycogen Synthase Kinase 3 beta/metabolism , Lens, Crystalline/metabolism , Mice, Knockout , Oxidative Stress , Proteomics , Proto-Oncogene Proteins c-akt/metabolism , Up-Regulation
3.
Toxicol Appl Pharmacol ; 473: 116599, 2023 08 15.
Article in English | MEDLINE | ID: mdl-37328116

ABSTRACT

Studies have indicated that glyphosate induces endocrine disruption and may adversely affect the male reproductive system. However, evidence of its effects on ovarian function is poorly understood so far, making further studies necessary on the mechanisms of the glyphosate toxicity in the female reproductive system. The aim of this work was to evaluate the effect of a subacute exposure (28 days) to the glyphosate-based formulation Roundup® (1.05, 10.5 and 105 µg/kg b.w. of glyphosate) on steroidogenesis, oxidative stress, systems involved in cell redox control and histopathological parameters in rat ovaries. Hence we quantify plasma estradiol and progesterone by chemiluminescence; non-protein thiol levels, TBARS, superoxide dismutase and catalase activity by spectrophotometry; gene expression of steroidogenic enzymes and redox systems by real-time PCR; and ovarian follicles by optical microscopy. Our results demonstrated that oral exposure increased progesterone levels and the mRNA expression of 3ß-hydroxysteroid dehydrogenase. Histopathological analysis revealed a decrease in the number of primary follicles and an increase in the number of corpus luteum in rats exposed to Roundup®. An imbalance of the oxidative status was also evidenced by decreasing the catalase activity at all groups exposed to the herbicide. Increased lipid peroxidation and gene expression of glutarredoxin and decreased of glutathione reductase were also observed. Our results indicate that Roundup® causes endocrine disruption of hormones related to female fertility and reproduction and changes the oxidative status by altering antioxidant activity, inducing lipid peroxidation, as well as changing the gene expression of the glutathione-glutarredoxin system in rat ovaries.


Subject(s)
Herbicides , Ovary , Rats , Male , Female , Animals , Progesterone , Catalase/genetics , Catalase/metabolism , Herbicides/toxicity , Glutaredoxins/pharmacology , Antioxidants/pharmacology , Glutathione/metabolism , Estradiol/pharmacology , Gene Expression , Glyphosate
4.
Metabolism ; 145: 155610, 2023 08.
Article in English | MEDLINE | ID: mdl-37277061

ABSTRACT

OBJECTIVE: Cholesterol gallstone disease (CGD) is closely related to cholesterol metabolic disorder. Glutaredoxin-1 (Glrx1) and Glrx1-related protein S-glutathionylation are increasingly being observed to drive various physiological and pathological processes, especially in metabolic diseases such as diabetes, obesity and fatty liver. However, Glrx1 has been minimally explored in cholesterol metabolism and gallstone disease. METHODS: We first investigated whether Glrx1 plays a role in gallstone formation in lithogenic diet-fed mice using immunoblotting and quantitative real-time PCR. Then a whole-body Glrx1-deficient (Glrx1-/-) mice and hepatic-specific Glrx1-overexpressing (AAV8-TBG-Glrx1) mice were generated, in which we analyzed the effects of Glrx1 on lipid metabolism upon LGD feeding. Quantitative proteomic analysis and immunoprecipitation (IP) of glutathionylated proteins were performed. RESULTS: We found that protein S-glutathionylation was markedly decreased and the deglutathionylating enzyme Glrx1 was greatly increased in the liver of lithogenic diet-fed mice. Glrx1-/- mice were protected from gallstone disease induced by a lithogenic diet because their biliary cholesterol and cholesterol saturation index (CSI) were reduced. Conversely, AAV8-TBG-Glrx1 mice showed greater gallstone progression with increased cholesterol secretion and CSI. Further studies showed that Glrx1-overexpressing greatly altered bile acid levels and/or composition to increase intestinal cholesterol absorption by upregulating Cyp8b1. In addition, liquid chromatography-mass spectrometry and IP analysis revealed that Glrx1 also affected the function of asialoglycoprotein receptor 1 (ASGR1) by mediating its deglutathionylation, thereby altering the expression of LXRα and controlling cholesterol secretion. CONCLUSION: Our findings present novel roles of Glrx1 and Glrx1-regulated protein S-glutathionylation in gallstone formation through the targeting of cholesterol metabolism. Our data advises Glrx1 significantly increased gallstone formation by simultaneously increase bile-acid-dependent cholesterol absorption and ASGR1- LXRα-dependent cholesterol efflux. Our work suggests the potential effects of inhibiting Glrx1 activity to treat cholelithiasis.


Subject(s)
Gallstones , Animals , Mice , Bile Acids and Salts/metabolism , Cholesterol/metabolism , Gallstones/metabolism , Glutaredoxins/genetics , Glutaredoxins/metabolism , Glutaredoxins/pharmacology , Lipid Metabolism/genetics , Liver/metabolism , Mice, Inbred C57BL , Protein S/metabolism , Protein S/pharmacology , Proteomics
5.
Molecules ; 26(11)2021 Jun 01.
Article in English | MEDLINE | ID: mdl-34206041

ABSTRACT

Parkinson's disease (PD) is characterized mainly by the loss of dopaminergic neurons in the substantia nigra (SN) mediated via oxidative stress. Although glutaredoxin-1 (GLRX1) is known as one of the antioxidants involved in cell survival, the effects of GLRX1 on PD are still unclear. In this study, we investigated whether cell-permeable PEP-1-GLRX1 inhibits dopaminergic neuronal cell death induced by 1-methyl-4-phenylpyridinium (MPP+) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). We showed that PEP-1-GLRX1 protects cell death and DNA damage in MPP+-exposed SH-SY5Y cells via the inhibition of MAPK, Akt, and NF-κB activation and the regulation of apoptosis-related protein expression. Furthermore, we found that PEP-1-GLRX1 was delivered to the SN via the blood-brain barrier (BBB) and reduced the loss of dopaminergic neurons in the MPTP-induced PD model. These results indicate that PEP-1-GLRX1 markedly inhibited the loss of dopaminergic neurons in MPP+- and MPTP-induced cytotoxicity, suggesting that this fusion protein may represent a novel therapeutic agent against PD.


Subject(s)
Cysteamine/analogs & derivatives , Dopaminergic Neurons/cytology , Glutaredoxins/administration & dosage , MAP Kinase Signaling System/drug effects , Parkinson Disease/drug therapy , Peptides/chemistry , 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine/adverse effects , 1-Methyl-4-phenylpyridinium/adverse effects , Animals , Apoptosis/drug effects , Cell Line , Cysteamine/chemistry , Disease Models, Animal , Dopaminergic Neurons/drug effects , Dopaminergic Neurons/metabolism , Gene Expression Regulation/drug effects , Glutaredoxins/chemistry , Glutaredoxins/pharmacology , Humans , Male , Mice , Parkinson Disease/etiology , Parkinson Disease/metabolism , Substantia Nigra/chemistry
6.
BMB Rep ; 53(2): 106-111, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31964467

ABSTRACT

Glutaredoxin 1 (GLRX1) has been recognized as an important regulator of redox signaling. Although GLRX1 plays an essential role in cell survival as an antioxidant protein, the function of GLRX1 protein in inflammatory response is still under investigation. Therefore, we wanted to know whether transduced PEP-1-GLRX1 protein inhibits lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. In LPS-exposed Raw 264.7 cells, PEP-1-GLRX1 inhibited cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), activation of mitogen activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) expression levels. In a TPA-induced mouse-ear edema model, topically applied PEP-1-GLRX1 transduced into ear tissues and significantly ameliorated ear edema. Our data reveal that PEP-1-GLRX1 attenuates inflammation in vitro and in vivo, suggesting that PEP-1-GLRX1 may be a potential therapeutic protein for inflammatory diseases. [BMB Reports 2020; 53(2): 106-111].


Subject(s)
Anti-Inflammatory Agents/pharmacology , Cysteamine/analogs & derivatives , Glutaredoxins/pharmacology , Inflammation/metabolism , Mitogen-Activated Protein Kinases/antagonists & inhibitors , NF-kappa B/metabolism , Peptides , Animals , Cyclooxygenase 2/metabolism , Cyclooxygenase 2 Inhibitors/pharmacology , Edema/chemically induced , Edema/metabolism , Edema/therapy , Lipopolysaccharides/pharmacology , Male , Mice , Mice, Inbred ICR , Mitogen-Activated Protein Kinases/metabolism , Nitric Oxide Synthase Type II/antagonists & inhibitors , Nitric Oxide Synthase Type II/metabolism , Phosphorylation , RAW 264.7 Cells , Signal Transduction/drug effects , Tetradecanoylphorbol Acetate/pharmacology
7.
Mol Med Rep ; 18(2): 2216-2228, 2018 Aug.
Article in English | MEDLINE | ID: mdl-29916538

ABSTRACT

Oxidative stress is known to be a primary risk factor for neuronal diseases. Glutaredoxin (GLRX)­1, a redox­regulator of the thioredoxin superfamily, is known to exhibit an important role in cell survival via various cellular functions. However, the precise roles of GLRX1 in brain ischemia are still not fully understood. The present study investigated whether transduced PEP­1­GLRX1 protein has protective effects against oxidative stress in cells and in an animal model. Transduced PEP­1­GLRX1 protein increased HT­22 cell viability under oxidative stress and this fusion protein significantly reduced intracellular reactive oxygen species and levels of DNA damage. In addition, PEP­1­GLRX1 protein regulated RAC­a serine/threonine­protein kinase and mitogen­activated protein kinase signaling, in addition to apoptotic signaling including B cell lymphoma (Bcl)­2, Bcl­2 associated X, apoptosis regulator, pro­caspase­9 and p53 expression levels. In an ischemic animal model, it was verified that PEP­1­GLRX1 transduced into the Cornu Ammonis 1 region of the animal brain, where it markedly protected against ischemic injury. These results indicate that PEP­1­GLRX1 attenuates neuronal cell death resulting from oxidative stress in vitro and in vivo. Therefore, PEP­1­GLRX1 may exhibit a beneficial role in the treatment of neuronal disorders, including ischemic injury.


Subject(s)
Cysteamine/analogs & derivatives , Glutaredoxins/pharmacology , Hippocampus/metabolism , MAP Kinase Signaling System/drug effects , Neurons/metabolism , Oxidative Stress/drug effects , Peptides/pharmacology , Animals , Brain Ischemia/drug therapy , Brain Ischemia/metabolism , Brain Ischemia/pathology , Cell Line , Cysteamine/pharmacology , Hippocampus/pathology , Mice , Neurons/pathology
8.
Exp Gerontol ; 104: 86-97, 2018 04.
Article in English | MEDLINE | ID: mdl-29414672

ABSTRACT

Glutaredoxin (Grx) is a polypeptide with low molecular weight, which has been extracted from buckwheat and has been suggested to have multiple functions revolving around oxidative stress responses and cell signaling. Here, we report the antioxidant activity of recombinant buckwheat Grx (rbGrx) to reduce aging effects in Caenorhabditis elegans (C. elegans) as well as the mechanism involved. Our results showed that rbGrx beneficially affected the health span of C. elegans, including pharyngeal-pumping rate, locomotion, and lipofuscin accumulation. Furthermore, stress assay showed that rbGrx could extend the lifespan under both oxidative and heat stress. Further studies indicated that the longevity-extending effects of rbGrx could be attributed to its in vitro and in vivo antioxidant activities. After treatment with rbGrx, SOD activity, CAT activity, GSH content, and GSH/GSSG ratio were increased, while MDA content was decreased, which led to low intracellular levels of reactive oxygen species in C. elegans. Moreover, rbGrx up-regulated hsf-1 mRNA level and could not expand the lifespan of the hsf-1 mutant C. elegans (sy441); however, this had no effect on the transcription of daf-16 and skn-1 and could expand the lifespan of both daf-16 and skn-1 mutants. These results suggested dependency of the rbGrx effect on the heat shock transcription factor (HSF-1) and independency on both DAF-16 and SKN-1. In summary, our results demonstrated the anti-aging activity of rbGrx, which increased resistance to cellular stress and improved the health span of C. elegans. These results are very important for the use of rbGrx in anti-aging research.


Subject(s)
Glutaredoxins/pharmacology , Longevity/drug effects , Animals , Antioxidants/physiology , Caenorhabditis elegans , Oxidative Stress/physiology , Recombinant Proteins/pharmacology , Up-Regulation
9.
Oxid Med Cell Longev ; 2017: 4162465, 2017.
Article in English | MEDLINE | ID: mdl-28706574

ABSTRACT

The general disruption of redox signaling following an ischemia-reperfusion episode has been proposed as a crucial component in neuronal death and consequently brain damage. Thioredoxin (Trx) family proteins control redox reactions and ensure protein regulation via specific, oxidative posttranslational modifications as part of cellular signaling processes. Trx proteins function in the manifestation, progression, and recovery following hypoxic/ischemic damage. Here, we analyzed the neuroprotective effects of postinjury, exogenous administration of Grx2 and Trx1 in a neonatal hypoxia/ischemia model. P7 Sprague-Dawley rats were subjected to right common carotid ligation or sham surgery, followed by an exposure to nitrogen. 1 h later, animals were injected i.p. with saline solution, 10 mg/kg recombinant Grx2 or Trx1, and euthanized 72 h postinjury. Results showed that Grx2 administration, and to some extent Trx1, attenuated part of the neuronal damage associated with a perinatal hypoxic/ischemic damage, such as glutamate excitotoxicity, axonal integrity, and astrogliosis. Moreover, these treatments also prevented some of the consequences of the induced neural injury, such as the delay of neurobehavioral development. To our knowledge, this is the first study demonstrating neuroprotective effects of recombinant Trx proteins on the outcome of neonatal hypoxia/ischemia, implying clinical potential as neuroprotective agents that might counteract neonatal hypoxia/ischemia injury.


Subject(s)
Asphyxia/complications , Glutaredoxins/therapeutic use , Hypoxia-Ischemia, Brain/metabolism , Neurons/pathology , Animals , Animals, Newborn , Disease Models, Animal , Glutaredoxins/administration & dosage , Glutaredoxins/pharmacology , Hypoxia-Ischemia, Brain/pathology , Male , Rats
10.
Free Radic Biol Med ; 51(8): 1501-11, 2011 Oct 15.
Article in English | MEDLINE | ID: mdl-21810465

ABSTRACT

Glutathionylation as a posttranslational modification of proteins is becoming increasingly recognized, but its role in many diseases has not been demonstrated. Oxidative stress and alterations in calcium homeostasis are associated with the development of cardiac hypertrophy. Because the cardiac L-type Ca(2+) channel can be persistently activated after exposure to H(2)O(2), the aim of this study was to determine whether alterations in channel function were associated with glutathionylation of the α(1C) subunit (Ca(v)1.2) channel protein. Immunoblot analysis indicated that Ca(v)1.2 protein is significantly glutathionylated after exposure to H(2)O(2) and glutathione in vitro and after ischemia-reperfusion injury. L-type Ca(2+) channel macroscopic current and intracellular calcium were significantly increased in myocytes after exposure to oxidized glutathione and reversed by glutaredoxin. The increase in current correlated with an increase in open probability of the channel assessed as changes in single-channel activity after exposing the human long N-terminal Ca(v)1.2 to H(2)O(2) or oxidized glutathione. We also demonstrate that the Ca(v)1.2 channel is significantly glutathionylated in ischemic human heart. We conclude that oxidative stress is associated with an increase in glutathionylation of the Ca(v)1.2 channel protein. We suggest that the associated constitutive activity contributes to the development of pathology in ischemic heart disease.


Subject(s)
Calcium Channels, L-Type/metabolism , Glutathione/metabolism , Heart/physiology , Myocytes, Cardiac/metabolism , Reperfusion Injury/metabolism , Animals , Calcium/metabolism , Cells, Cultured , Disease Models, Animal , Electric Conductivity , Glutaredoxins/pharmacology , Glutathione/chemistry , Guinea Pigs , Heart/drug effects , Humans , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/pathology , Oxidative Stress/drug effects , Protein Processing, Post-Translational/drug effects , Protein Processing, Post-Translational/physiology , Reperfusion Injury/pathology , Reperfusion Injury/physiopathology
11.
J Biol Chem ; 285(49): 38641-8, 2010 Dec 03.
Article in English | MEDLINE | ID: mdl-20926382

ABSTRACT

The K(ATP) channel is an important player in vascular tone regulation. Its opening and closure lead to vasodilation and vasoconstriction, respectively. Such functions may be disrupted in oxidative stress seen in a variety of cardiovascular diseases, while the underlying mechanism remains unclear. Here, we demonstrated that S-glutathionylation was a modulation mechanism underlying oxidant-mediated vascular K(ATP) channel regulation. An exposure of isolated mesenteric rings to hydrogen peroxide (H(2)O(2)) impaired the K(ATP) channel-mediated vascular dilation. In whole-cell recordings and inside-out patches, H(2)O(2) or diamide caused a strong inhibition of the vascular K(ATP) channel (Kir6.1/SUR2B) in the presence, but not in the absence, of glutathione (GSH). Similar channel inhibition was seen with oxidized glutathione (GSSG) and thiol-modulating reagents. The oxidant-mediated channel inhibition was reversed by the reducing agent dithiothreitol (DTT) and the specific deglutathionylation reagent glutaredoxin-1 (Grx1). Consistent with S-glutathionylation, streptavidin pull-down assays with biotinylated glutathione ethyl ester (BioGEE) showed incorporation of GSH to the Kir6.1 subunit in the presence of H(2)O(2). These results suggest that S-glutathionylation is an important mechanism for the vascular K(ATP) channel modulation in oxidative stress.


Subject(s)
Glutathione Disulfide/metabolism , Mesenteric Arteries/metabolism , Oxidative Stress , Potassium Channels, Inwardly Rectifying/metabolism , Animals , Cardiovascular Diseases/metabolism , Dithiothreitol/pharmacology , Glutaredoxins/metabolism , Glutaredoxins/pharmacology , Glutathione Disulfide/pharmacology , Humans , Hydrogen Peroxide/pharmacology , KATP Channels , Male , Oxidants/pharmacology , Rats , Rats, Sprague-Dawley , Vasodilation/drug effects , Vasodilation/physiology
12.
Am J Physiol Lung Cell Mol Physiol ; 299(2): L192-203, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20472709

ABSTRACT

Glutaredoxin 1 (Glrx1) is a small dithiol protein that regulates the cellular redox state and redox-dependent signaling pathways via modulation of protein glutathionylation. IkappaB kinase (IKK), an essential enzyme for NF-kappaB activation, can be subjected to S-glutathionylation leading to alteration of its activity. However, the role of Glrx1 in cigarette smoke (CS)-induced lung inflammation and chromatin modifications are not known. We hypothesized that Glrx1 regulates the CS-induced lung inflammation and chromatin modifications via differential regulation of IKKs by S-glutathionylation in mouse lung. Glrx1 knockout (KO) and wild-type (WT) mice were exposed to CS for 3 days and determined the role of Glrx1 in regulation of proinflammatory response in the lung. Neutrophil influx in bronchoalveolar lavage fluid and proinflammatory cytokine release in lung were increased in Glrx1 KO mice compared with WT mice exposed to CS, which was associated with augmented nuclear translocation of RelA/p65 and its phospho-acetylation. Interestingly, phosphorylated and total levels of IKKalpha, but not total and phosphorylated IKKbeta levels, were increased in lungs of Glrx1 KO mice compared with WT mice exposed to CS. Ablation of Glrx1 leads to increased CS-induced IKKbeta glutathionylation rendering it inactive, whereas IKKalpha was activated resulting in increased phospho-acetylation of histone H3 in mouse lung. Thus, targeted disruption of Glrx1 regulates the lung proinflammatory response via histone acetylation specifically by activation of IKKalpha in response to CS exposure. Overall, our study suggests that S-glutathionylation and phosphorylation of IKKalpha plays an important role in histone acetylation on proinflammatory gene promoters and NF-kappaB-mediated abnormal and sustained lung inflammation in pathogenesis of chronic inflammatory lung diseases.


Subject(s)
Glutaredoxins/pharmacology , Histones/metabolism , NF-kappa B/metabolism , Smoking/adverse effects , Acetylation , Animals , Bronchoalveolar Lavage Fluid/cytology , Glutaredoxins/biosynthesis , Glutaredoxins/deficiency , Glutathione/metabolism , I-kappa B Kinase/metabolism , Mice , Mice, Knockout , Pneumonia/etiology , Pneumonia/pathology , Pneumonia/prevention & control
13.
Int J Biochem Cell Biol ; 41(6): 1269-75, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19038358

ABSTRACT

Reduction of intramolecular disulfides in the HIV-1 envelope protein gp120 occurs after its binding to the CD4 receptor. Protein disulfide isomerase (PDI) catalyzes the disulfide reduction in vitro and inhibition of this enzyme blocks viral entry. PDI belongs to the thioredoxin protein superfamily that also includes human glutaredoxin-1 (Grx1). Grx1 is secreted from cells and the protein has also been found within the HIV-1 virion. We show that Grx1 efficiently catalyzes gp120, and CD4 disulfide reduction in vitro, even at low plasma levels of glutathione. Grx1 catalyzes the reduction of two disulfide bridges in gp120 in a similar manner as PDI. Purified anti-Grx1 antibodies were shown to inhibit the Grx1 activity in vitro and block HIV-1 replication in cultured peripheral blood mononuclear cells. Also, the polyanion PRO2000, that was previously shown to prevent HIV entry, inhibits the Grx1- and PDI-dependent reduction of gp120 disulfides. Our findings suggest that Grx1 activity is important for HIV-1 entry and that Grx1 and the gp120 intramolecular disulfides are novel pharmacological targets for rational drug development.


Subject(s)
CD4 Antigens/metabolism , Glutaredoxins/metabolism , HIV Envelope Protein gp120/metabolism , HIV-1/physiology , Virus Replication/physiology , Animals , Catalysis , Cattle , Disulfides/metabolism , Glutaredoxins/antagonists & inhibitors , Glutaredoxins/pharmacology , Glutathione/metabolism , HIV-1/metabolism , Humans , Naphthalenesulfonates/pharmacology , Oxidation-Reduction , Polymers/pharmacology , Protein Disulfide-Isomerases/metabolism , Recombinant Proteins/pharmacology , Virus Internalization/drug effects , Virus Replication/drug effects
14.
Invest Ophthalmol Vis Sci ; 49(10): 4497-505, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18586881

ABSTRACT

PURPOSE: To examine the physiological function of the thioltransferase (TTase)/glutathione (GSH) system in the lens using TTase knockout mouse (TTase(-/-)) lens epithelial cells (LECs) as a model. METHODS: Primary LEC cultures were obtained from wild-type (TTase(+/+)) and TTase(-/-) mice. Characterization and validation of the cells were determined by immunoblotting for TTase and alpha-crystallin proteins and by immunohistochemistry for glutathionylated proteins. Cell proliferation was examined by 3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium and BrdU analysis, and cell apoptosis after H(2)O(2) stress was assessed by fluorescence-activated cell sorter analysis. Reloading of TTase protein into the TTase(-/-) cells was achieved with reagent. RESULTS: Primary LEC cultures obtained from wild-type (TTase(+/+)) and TTase(-/-) mice were characterized and found to contain lens-specific alpha-crystallin protein. Western blot analysis confirmed the absence of TTase protein in the TTase(-/-) cells and its presence in the wild-type cells. TTase(-/-) LECs had significantly lower levels of glutathione (GSH) and protein thiols with extensive elevation of glutathionylated proteins, and they exhibited less resistance to oxidative stress than did TTase(+/+) cells. These cells were less viable and more apoptotic, and they had a reduced ability to remove H(2)O(2) after challenge with low levels of H(2)O(2). Reloading of purified TTase into the TTase(-/-) cells restored the antioxidant function in TTase(-/-) cells to a near normal state. CONCLUSIONS: These findings confirm the importance of TTase in regulating redox homeostasis and suggest a new physiological function in controlling cell proliferation in the lens epithelial cells.


Subject(s)
Cell Proliferation , Glutaredoxins/physiology , Lens, Crystalline/cytology , Oxidative Stress , Animals , Apoptosis , Blotting, Northern , Blotting, Western , Cell Survival , Cells, Cultured , Cytosol , Epithelial Cells/cytology , Epithelial Cells/metabolism , Flow Cytometry , Glutaredoxins/pharmacology , Glutathione/physiology , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , Hydrogen Peroxide/toxicity , Lens, Crystalline/metabolism , Mice , Mice, Inbred C57BL , Mice, Knockout , Recombinant Proteins/pharmacology , alpha-Crystallin A Chain/metabolism
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