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Rev Latinoam Microbiol ; 35(3): 259-65, 1993.
Article in Spanish | MEDLINE | ID: mdl-8047728

ABSTRACT

Three extracts from Saccharomyces cerevisiae were obtained by salting out with ammonium sulfate, these were I-G, EFRL-II-G and III-G. Fraction EFRL-II-G showed the highest photoreactivating activity on DNA str2000 irradiated with far UV light. However, the same fraction did not reactivate DNA str2000 previously inactivated by near UV irradiation. We think that the inactivation by near-UV was not due to photochemically-formed pyrimidine dimers. Decrease in spontaneous mutation frequency of cells transformed with DNA str2000 irradiated with near-UV light, was the same with the DNA treated with active or heat inactivated EFRL-II-G, therefore we may conclude that DNA lesions responsible for the effect are difference to pyrimidine dimers.


Subject(s)
DNA Damage , DNA Repair , DNA, Bacterial/radiation effects , Haemophilus influenzae/genetics , Transformation, Bacterial/radiation effects , Ultraviolet Rays , Deoxyribodipyrimidine Photo-Lyase/isolation & purification , Deoxyribodipyrimidine Photo-Lyase/metabolism , Fungal Proteins/isolation & purification , Fungal Proteins/metabolism , Haemophilus influenzae/radiation effects , Mutagenesis , Pyrimidine Dimers/chemistry , Saccharomyces cerevisiae/enzymology
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