ABSTRACT
In this study, two halophilic bacterial strains isolated from saline habitats in Argentina grew in the presence of gas oil. They were identified as Halomonas spp. and Nesterenkonia sp. by 16S ribosomal RNA sequencing. Chemotaxis towards gas oil was observed in Halomonas spp. by using swimming assays.
Subject(s)
Chemotaxis/drug effects , Halomonas/drug effects , Micrococcaceae/drug effects , Petroleum , Argentina , Biodegradation, Environmental , Halomonas/growth & development , Halomonas/isolation & purification , Hydrocarbons , Micrococcaceae/growth & development , Micrococcaceae/isolation & purification , Ribotyping , Salt Tolerance , Seawater/microbiology , Species Specificity , Water Microbiology , Water Pollutants, ChemicalABSTRACT
In this study, two halophilic bacterial strains isolated from saline habitats in Argentina grew in the presence of gas oil. They were identified as Halomonas spp. and Nesterenkonia sp. by 16S ribosomal RNA sequencing. Chemotaxis towards gas oil was observed in Halomonas spp. by using swimming assays.
En el presente trabajo se aislaron dos cepas bacterianas halofílicas a partir de muestras obtenidas en ambientes salinos de Argentina, que crecieron en presencia de gasoil como única fuente de carbono. Las cepas aisladas se identificaron como Halomonas spp. y Nesterenkonia sp. mediante secuenciación del gen del ARN ribosomal 16S. En ensayos de swimming, las cepas del genero Halomonas spp. mostraron una respuesta quimiotáctica hacia el gas oil.
Subject(s)
Chemotaxis/drug effects , Halomonas/drug effects , Micrococcaceae/drug effects , Petroleum , Argentina , Biodegradation, Environmental , Hydrocarbons , Halomonas/growth & development , Halomonas/isolation & purification , Micrococcaceae/growth & development , Micrococcaceae/isolation & purification , Ribotyping , Salt Tolerance , Species Specificity , Seawater/microbiology , Water Microbiology , Water Pollutants, ChemicalABSTRACT
Marine biofilms are a virtually untapped source of bioactive molecules that may find application as novel antifoulants in the marine paint industry. This study aimed at determining the potential of marine biofilm bacteria to produce novel biomolecules with potential application as natural antifoulants. Nine representative strains were isolated from a range of surfaces and were grown in YEB medium and harvested during the late exponential growth phase. Bacterial biomass and spent culture medium were extracted with ethanol and ethyl acetate, respectively. Extracts were assayed for their antifouling activity using two tests: (1) antimicrobial well diffusion test against a common fouling bacterium, Halomonas marina, and (2) anti-crustacean activity test using Artemia salina. Our results showed that none of the ethanolic extracts (bacterial biomass) were active in either test. In contrast, most of the organic extracts had antimicrobial activity (88%) and were toxic towards A. salina (67%). Sequencing of full 16 S ribosomal DNA analysis showed that the isolates were related to Bacillus mojavensis and Bacillus firmus. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) profiling of ethyl acetate extracts of culture supernatants showed that these species produce the bioactive lipopeptides surfactin A, mycosubtilin and bacillomycin D.