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1.
Curr Protoc Pharmacol ; 69: 14.34.1-14.34.35, 2015 Jun 01.
Article in English | MEDLINE | ID: mdl-26344212

ABSTRACT

Gastric adenocarcinoma is the fifth most common cancer and third most common cause of cancer-related death in the world. The majority of these cancers develop in genetically susceptible individuals who are chronically infected with the Gram-negative bacterium Helicobacter pylori. Often these individuals have also been exposed to certain environmental factors that increase susceptibility, such as dietary components. Murine models of Helicobacter-induced gastric cancer are valuable tools for investigating the mechanisms responsible for the stepwise pathological changes of chronic atrophic gastritis, intestinal metaplasia, dysplasia and gastric adenocarcinoma. Helicobacter felis colonization greatly accelerates the development of gastric neoplasia in mice, and causes pathologies similar to those observed with Helicobacter pylori-associated gastric carcinogenesis in humans. These mouse models are therefore useful for investigating genetic and environmental factors that may be involved in the pathogenesis and treatment of gastric cancer. Detailed in these protocols are procedures for inducing Helicobacter-associated carcinogenesis in mice as well as the histological analysis and interpretation of gastric pathology in these animals.


Subject(s)
Adenocarcinoma/microbiology , Disease Models, Animal , Helicobacter Infections/microbiology , Helicobacter felis/pathogenicity , Helicobacter pylori/pathogenicity , Paraneoplastic Syndromes/microbiology , Stomach Neoplasms/microbiology , Adenocarcinoma/drug therapy , Adenocarcinoma/immunology , Adenocarcinoma/pathology , Animals , Anti-Bacterial Agents/therapeutic use , Antineoplastic Agents/therapeutic use , Carcinogenesis/drug effects , Carcinogenesis/immunology , Carcinogenesis/metabolism , Carcinogenesis/pathology , Gastric Mucosa/drug effects , Gastric Mucosa/immunology , Gastric Mucosa/microbiology , Gastric Mucosa/pathology , Helicobacter Infections/drug therapy , Helicobacter Infections/immunology , Helicobacter Infections/pathology , Helicobacter felis/drug effects , Helicobacter felis/immunology , Helicobacter pylori/drug effects , Helicobacter pylori/immunology , Humans , Mice , Paraneoplastic Syndromes/drug therapy , Paraneoplastic Syndromes/immunology , Paraneoplastic Syndromes/pathology , Stomach Neoplasms/drug therapy , Stomach Neoplasms/immunology , Stomach Neoplasms/pathology
2.
Gastroenterology ; 145(5): 1045-54, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23860501

ABSTRACT

BACKGROUND & AIMS: Dual oxidases (DUOX) are conserved reduced nicotinamide adenine dinucleotide phosphate oxidases that produce H2O2 at the epithelial cell surface. The DUOX enzyme comprises the DUOX and DUOX maturation factor (DUOXA) subunits. Mammalian genomes encode 2 DUOX isoenzymes (DUOX1/DUOXA1 and DUOX2/DUOXA2). Expression of these genes is up-regulated during bacterial infections and chronic inflammatory diseases of the luminal gastrointestinal tract. The roles of DUOX in cellular interactions with microbes have not been determined in higher vertebrates. METHODS: Mice with disruptions of Duoxa1 and Duoxa2 genes (Duoxa(-/-) mice) and control mice were infected with Helicobacter felis to create a model of Helicobacter pylori infection--the most common human chronic infection. RESULTS: Infection with H. felis induced expression of Duox2 and Duoxa2 in the stomachs of wild-type mice, and DUOX protein specifically localized to the apical surface of epithelial cells. H. felis colonized the mucus layer in the stomachs of Duoxa(-/-) mice to a greater extent than in control mice. The increased colonization persisted into the chronic phase of infection and correlated with an increased, yet ineffective, inflammatory response. H. felis colonization also was increased in Duoxa(+/-) mice, compared with controls. We observed reduced expression of the H2O2-inducible katA gene in H. felis that colonized Duoxa(-/-) mice, compared with that found in controls (P = .0002), indicating that Duox causes oxidative stress in these bacteria. In vitro, induction of oxidative defense by H. felis failed to prevent a direct bacteriostatic effect at sustained levels of H2O2 as low as 30 µmol/L. CONCLUSIONS: Based on studies of Duoxa(-/-) mice, the DUOX enzyme complex prevents gastric colonization by H. felis and the inflammatory response. These findings indicate the nonredundant function of epithelial production of H2O2 in restricting microbial colonization.


Subject(s)
Gastric Mucosa/metabolism , Gastritis/prevention & control , Helicobacter Infections/prevention & control , Helicobacter felis , Hydrogen Peroxide/metabolism , Membrane Proteins/metabolism , Nerve Tissue Proteins/metabolism , Nuclear Proteins/metabolism , Animals , Disease Models, Animal , Dose-Response Relationship, Drug , Epithelium/metabolism , Epithelium/microbiology , Female , Gastric Mucosa/microbiology , Gastritis/metabolism , Helicobacter Infections/metabolism , Helicobacter felis/drug effects , Hydrogen Peroxide/pharmacology , In Vitro Techniques , Membrane Proteins/deficiency , Membrane Proteins/genetics , Mice , Mice, Knockout , Nerve Tissue Proteins/deficiency , Nerve Tissue Proteins/genetics , Nuclear Proteins/deficiency , Nuclear Proteins/genetics , Stomach/microbiology , Up-Regulation
3.
Int J Antimicrob Agents ; 33(5): 473-8, 2009 May.
Article in English | MEDLINE | ID: mdl-19157800

ABSTRACT

Helicobacter infection, one of the most common bacterial infections in man worldwide, is a type 1 carcinogen and the most important risk factor for gastric cancer. Helicobacter pylori bacterial factors, components of the host genetics and immune response, dietary cofactors and decreased acid secretion resulting in bacterial overgrowth are all considered important factors for induction of gastric cancer. Components found in green tea have been shown to inhibit bacterial growth, including the growth of Helicobacter spp. In this study, we assessed the bactericidal and/or bacteriostatic effect of green tea against Helicobacter felis and H. pylori in vitro and evaluated the effects of green tea on the development of Helicobacter-induced gastritis in an animal model. Our data clearly demonstrate profound growth effects of green tea against Helicobacter and, importantly, demonstrate that green tea consumption can prevent gastric mucosal inflammation if ingested prior to exposure to Helicobacter infection. Research in the area of natural food compounds and their effects on various disease states has gained increased acceptance in the past several years. Components within natural remedies such as green tea could be further used for prevention and treatment of Helicobacter-induced gastritis in humans.


Subject(s)
Anti-Bacterial Agents/therapeutic use , Helicobacter Infections/prevention & control , Helicobacter felis/drug effects , Helicobacter pylori/drug effects , Plant Extracts/therapeutic use , Tea/chemistry , Animals , Anti-Bacterial Agents/pharmacology , Gastric Mucosa/pathology , Gastritis/prevention & control , Humans , Male , Mice , Plant Extracts/pharmacology
4.
Helicobacter ; 11(5): 460-8, 2006 Oct.
Article in English | MEDLINE | ID: mdl-16961809

ABSTRACT

BACKGROUND: Helicobacter pylori is a causative agent of gastric and duodenal ulcers and gastric cancer. Its urease enzyme allows survival in acid conditions and drives bacterial intracellular metabolism. We aimed to investigate the role of urease in determining the intragastric distribution of Helicobacter species in vivo. MATERIALS AND METHODS: The C57BL/6 mouse model of gastritis was used for infection with Helicobacter felis (CS1) or H. pylori (SS1). Urease-modulating compounds urea and/or fluorofamide (urease inhibitor) were administered to mice over 7 days. Concurrent gastric acid inhibition by omeprazole was also examined. Bacterial distribution in the antrum, body, antrum/body, and body/cardia transitional zones was graded "blindly" by histologic evaluation. Bacterial colony counts on corresponding tissue were also conducted. RESULTS: Urease inhibition by fluorofamide decreased H. pylori survival in most gastric regions (p < .05); however, there were no marked changes to H. felis colonization after this treatment. There was a consistent trend for decreased antral colonization, and an increase in antrum/body transitional zone and body colonization with excess 5% or 6% (w/v) urea treatment. Significant reductions of both Helicobacter species were observed with the co-treatment of urea and fluorofamide (p < .05). Collateral treatment with omeprazole did not alter H. pylori colonization patterns caused by urea/fluorofamide. CONCLUSIONS: Urease perturbations affect colonization patterns of Helicobacter species. Combined urea and fluorofamide treatment reduced the density of both Helicobacter species in our infection model.


Subject(s)
Anti-Bacterial Agents/therapeutic use , Benzamides/therapeutic use , Gastric Mucosa/microbiology , Helicobacter Infections/drug therapy , Helicobacter pylori/drug effects , Omeprazole/therapeutic use , Urea/therapeutic use , Urease/antagonists & inhibitors , Animals , Anti-Ulcer Agents/administration & dosage , Anti-Ulcer Agents/therapeutic use , Benzamides/administration & dosage , Enzyme Inhibitors/administration & dosage , Enzyme Inhibitors/therapeutic use , Female , Gastric Mucosa/drug effects , Helicobacter Infections/microbiology , Helicobacter felis/drug effects , Helicobacter felis/enzymology , Helicobacter pylori/enzymology , Hydrogen-Ion Concentration , Mice , Mice, Inbred BALB C , Models, Animal , Urea/administration & dosage , Urease/metabolism
5.
Antimicrob Agents Chemother ; 49(7): 2997-3000, 2005 Jul.
Article in English | MEDLINE | ID: mdl-15980383

ABSTRACT

The susceptibilities of Helicobacter felis (15 strains), H. bizzozeronii (7 strains), and H. salomonis (3 strains) to 10 antimicrobial agents were investigated by determination of the MIC using the agar dilution method. No consistent differences were noticed between the different Helicobacter species, which were all highly susceptible to ampicillin, clarithromycin, tetracycline, tylosin, enrofloxacin, gentamicin, and neomycin, as demonstrated by low MICs. Higher MICs were obtained for lincomycin (up to 8 microg/ml) and spectinomycin (up to 4 microg/ml). Two H. felis strains showed a MIC of 16 microg/ml for metronidazole, suggesting acquired resistance to this antimicrobial agent.


Subject(s)
Anti-Bacterial Agents/pharmacology , Cat Diseases/microbiology , Dog Diseases/microbiology , Helicobacter Infections/microbiology , Helicobacter felis/drug effects , Helicobacter/drug effects , Animals , Cats , Dogs , Drug Resistance, Bacterial , Helicobacter/classification , Humans , Metronidazole/pharmacology , Microbial Sensitivity Tests/methods , Microbial Sensitivity Tests/standards
6.
Dig Dis Sci ; 49(11-12): 1853-61, 2004.
Article in English | MEDLINE | ID: mdl-15628716

ABSTRACT

N-Acetylcysteine (NAC), being both a mucolytic agent and a thiol-containing antioxidant, may affect the establishment and maintenance of H. pylori infection within the gastric mucus layer and mucosa. Agar and broth dilution susceptibility tests determined the MIC of H. pylori strain SSI to NAC. H. pylori load in SSI strain-infected C57BL mice was determined as colony forming units per gram of gastric tissue. Gastritis assessment was scored and gastric surface hydrophobicity was determined by contact angle measurement. MICs of NAC were 5 to 10 and 10 to 15 mg/ml using the agar dilution and broth dilution methods, respectively. NAC (120 mg per day for 14 days) reduced the H. pylori load in mice by almost 1 log compared with sham treatment. Pretreatment with NAC (40 mg/day) also significantly reduced the H. pylori load but did not prevent H. pylori colonization. Both H. pylori infection and NAC reduced the surface hydrophobicity of murine gastric mucosa. No significant differences were observed in the gastritis scores of H. felis- or H. pylori-infected mice receiving either NAC or sham treatments. This study demonstrates that NAC inhibits the growth of H. pylori in both agar and broth susceptibility tests and in H. pylori-infected mice. NAC did not alter the severity of H. pylori- or H. felis-induced gastritis.


Subject(s)
Acetylcysteine/therapeutic use , Antioxidants/therapeutic use , Expectorants/therapeutic use , Helicobacter Infections/drug therapy , Helicobacter pylori/drug effects , Animals , Drug Resistance, Bacterial , Female , Gastric Mucosa/drug effects , Gastric Mucosa/microbiology , Gastritis/drug therapy , Gastritis/microbiology , Helicobacter felis/drug effects , Hydrophobic and Hydrophilic Interactions , Mice , Mice, Inbred C57BL , Time Factors
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