ABSTRACT
(-)-Securinine (SE) is a major alkaloid found in plant Securinega suffruticosa, which has a wide range of pharmacological activities including anticancer, anti-parasitic and central nervous system stimulating effects, etc. To aid the pharmacological study of SE, we developed an LC-MS/MS method for quantitative determination of SE in mouse plasma. In this method, plasma samples were first prepared with salting-out assisted liquid-liquid extraction using cold acetonitrile (-20°C) and 2.00 M ammonium acetate. Separation of SE and the internal standard (IS) from sample matrix was achieved on a Gemini Nx C18 column using 40% acetonitrile and 60% 10.0mM ammonium acetate at a flow rate of 0.200 mL min(-1). Quantification of SE was accomplished with positive electrospray ionization tandem mass spectrometry using mass transitions m/z 218.1â84.1 for SE, and m/z 204.1â70.2 for the IS. This method has a lower limit of quantitation (LLOQ) of 0.600 ng mL(-1) and a linear calibration range up to 600 ng mL(-1) in mouse plasma. The intra- and inter-run accuracy (%RE) and precision (%CV) were ≤ ± 6% and 6%, respectively. The IS normalized matrix factors from six lots of plasma matrices ranged 0.92-1.07, and the recoveries of plasma SE were 99-109%. The validated method has been applied to the measurement of SE in plasma samples of a mouse study.
Subject(s)
Azepines/blood , Chromatography, Reverse-Phase/methods , Heterocyclic Compounds, Bridged-Ring/blood , Lactones/blood , Piperidines/blood , Tandem Mass Spectrometry/methods , Animals , Azepines/chemistry , Azepines/isolation & purification , Azepines/pharmacokinetics , Heterocyclic Compounds, Bridged-Ring/chemistry , Heterocyclic Compounds, Bridged-Ring/isolation & purification , Heterocyclic Compounds, Bridged-Ring/pharmacokinetics , Lactones/chemistry , Lactones/isolation & purification , Lactones/pharmacokinetics , Linear Models , Liquid-Liquid Extraction , Mice , Mice, Inbred BALB C , Piperidines/chemistry , Piperidines/isolation & purification , Piperidines/pharmacokinetics , Reproducibility of Results , Sensitivity and Specificity , Solubility , Spectrometry, Mass, Electrospray Ionization/methodsABSTRACT
The synthesis and biological testing of a novel series of nonpeptide vasopressin receptor antagonists, containing a bridged bicyclic nucleus, are reported. Variation of substituents (R(1)-R(3)) in general formula 3, and the configuration of the stereocenter, resulted in potent V(2)-selective (e.g., 5) and balanced dual V(1a)/V(2) (e.g., 10) compounds. Data from receptor binding, cell-based functional, and in vivo assays are presented [corrected]
