Subject(s)
Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Hodgkin Disease , Schistosoma mansoni , Schistosomiasis mansoni , Animals , Child , Cyclophosphamide/administration & dosage , Dacarbazine/administration & dosage , Hodgkin Disease/diagnosis , Hodgkin Disease/drug therapy , Hodgkin Disease/parasitology , Hodgkin Disease/pathology , Humans , Male , Prednisolone/administration & dosage , Schistosomiasis mansoni/diagnosis , Schistosomiasis mansoni/drug therapy , Schistosomiasis mansoni/pathology , Vincristine/administration & dosageSubject(s)
Bone Marrow Diseases/parasitology , Filariasis/parasitology , Hodgkin Disease/parasitology , Microfilariae/growth & development , Wuchereria bancrofti/growth & development , Adult , Animals , Humans , Male , Microfilariae/isolation & purification , Neck , Wuchereria bancrofti/isolation & purificationABSTRACT
The case of an HIV-positive man treated for acute toxoplasmosis with no traces of malignancy is reported. A second lymph node extirpation was performed after 5 months, which identified the presence of Hodgkin and Reed-Sternberg (HRS) cells. This case suggests that toxoplasmosis may cause changes in the regulation of surrounding cells and induce neoplastic proliferation.
Subject(s)
Hodgkin Disease/parasitology , Toxoplasmosis/complications , Adult , HIV Infections/complications , HIV Infections/parasitology , HIV Infections/pathology , Hodgkin Disease/pathology , Hodgkin Disease/virology , Humans , Male , Reed-Sternberg Cells/cytology , Toxoplasmosis/pathology , Toxoplasmosis/virologySubject(s)
Hodgkin Disease/parasitology , Strongyloides stercoralis , Strongyloidiasis , Adult , Animals , Humans , MaleABSTRACT
Using both traditional methods and broad-range 18S ribosomal DNA (rDNA) polymerase chain reaction, we examined 2 cases of lethal cestodiasis, in which the disease agent had been poorly identified or misidentified. In one case, involving a patient with AIDS, we identified the human dwarf tapeworm, Hymenolepis nana, as a cause of aberrant metastatic larval disease. In the second case with similar pathologic abnormalities, involving a patient with Hodgkin disease, we identified a larval cestode with a previously uncharacterized 18S rDNA sequence. A prior report of this case nearly 30 years ago, based on tissue examination, had suggested that the parasite was a sparganum.
Subject(s)
Acquired Immunodeficiency Syndrome/complications , Acquired Immunodeficiency Syndrome/immunology , Cestode Infections/complications , Cestode Infections/immunology , Hodgkin Disease/complications , Hodgkin Disease/immunology , Acquired Immunodeficiency Syndrome/parasitology , Animals , Cestoda/genetics , Cestoda/isolation & purification , DNA, Ribosomal/analysis , Fatal Outcome , Hodgkin Disease/parasitology , Humans , Male , Opportunistic Infections/complications , Opportunistic Infections/immunology , Opportunistic Infections/parasitology , Phylogeny , RNA, Ribosomal, 18S/analysisABSTRACT
AIM: To detect the presence of Toxoplasma gondii in lymph node tissue in patients with Toxoplasma infection. METHODS: T. gondii (RH strain) specific DNA fragment clones were obtained by using PCR and gene recombination technique. The DNA fragments used as hybridization probes were labelled with digoxigenin by random primer method. The technique of in situ hybridization (ISH) was used to detect T. g DNA in the lymph node sections. RESULTS: Four out of 120 samples T. g DNA were found positive, one with Hodgkin's disease (HD) (1/32), one with non-Hodgkin's lymphoma (NHL) (1/41) and 2 with chronic lymphadenitis (CL) (2/47). The total positive rate was 3.3%. It was demonstrated that this highly specific probe could detect 10 pg of the total RH strain T. g DNA. CONCLUSION: ISH was applicable in detecting pathogens in the lymph node tissues of individuals with Toxoplasma infection.
Subject(s)
DNA, Protozoan/analysis , Lymph Nodes/parasitology , Toxoplasma/isolation & purification , Toxoplasmosis/parasitology , Animals , Hodgkin Disease/parasitology , Humans , In Situ Hybridization , Lymphoma, Non-Hodgkin/parasitologyABSTRACT
In order to investigate the morbidity of toxoplasmic lymphadenitis, polymerase chain reaction (PCR) was used to detect DNA of Toxoplasma gondii within lymph nodes in 3 groups of 120 patients with different diseases. After extracting the DNA of each sample, PCR was employed to amplify toxoplasma DNA. The results showed that the amplification product of 210 bp was confirmed in 7 patients: 3 cases of Hodgkin's disease (HD), 2 cases of non-Hodgkin's lymphoma (NHL) and 2 cases of chronic lymphadenitis (CL). Each PCR product was then subjected to Southern blot hybridization. Besides the 7 cases proved by PCR, 1 case of CL was found positive. The positive percentages of HD, NHL and CL were 9.38% (3/32), 4.88% (2/41), and 6.38% (3/47), respectively. The total positive rate was 6.67% (8/120).
Subject(s)
DNA, Protozoan/isolation & purification , Lymph Nodes/parasitology , Lymphadenitis/parasitology , Toxoplasma/isolation & purification , Animals , Chronic Disease , Female , Hodgkin Disease/parasitology , Humans , Lymphoma, Non-Hodgkin/parasitology , Male , Polymerase Chain Reaction , Toxoplasmosis/parasitologyABSTRACT
Hodgkin's disease (HD) is a specific type of malignant lymphoma characteristic of local and general lymphadenectasis. Aquired toxoplasmosis (AT) is one kind of lymphoadenopathy without fever and fatigue. When the two diseases coexist, clinical and pathological misdiagnosis may be made. This is the first male case of toxoplasmosis and Hodgkin's disease in China, diagnosed by surgical removal of the major part of the cervical and supraclavicular masses, detection of blood anti-toxoplasma gondii antibody, PCR analysis of toxoplasma gondii DNA, and pathological, ultrastructural and immunohistochemical studies of the tumour tissues. The patient treated by radiation and chemotherapy was abated.
Subject(s)
Hodgkin Disease/parasitology , Toxoplasmosis/therapy , Adult , Combined Modality Therapy , Hodgkin Disease/therapy , Humans , MaleABSTRACT
The autopsy of a man who died of Hodgkin disease revealed that a peculiar metazoan parasite had proliferated and disseminated throughout his body. The parasite could not be identified; however, electron microscopical studies revealed that it had the structure of a flatworm. This, together with its shape and structure, convinced us that the parasite was an aberrant sparganum manifesting uncontrolled proliferation and dissemination.
